The Swemed Total Protein Reagent Kit 2 × 50 ml is a professional clinical biochemistry reagent intended for the photometric quantitative determination of total protein in serum and plasma using the Biuret Method. Swemed lists the 2 × 50 ml pack as reference TPR100, with a second 2 × 100 ml presentation also available.
In the Biuret reaction, proteins in an alkaline medium bind with cupric ions to form a blue-violet coloured complex. The intensity of the colour produced is directly proportional to the amount of protein present in the sample.
The reagent is supplied as a ready-to-use liquid reagent, together with a Total Protein standard. It is suitable for routine use in pathology laboratories, hospitals, diagnostic centres and clinical chemistry laboratories.
| Product Detail | Information |
|---|---|
| Product Name | Total Protein Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Manufacturer Reference | TPR100 |
| Product Type | Clinical Biochemistry Reagent |
| Test Parameter | Total Protein |
| Method | Biuret Method |
| Measurement | Photometric / Colorimetric |
| Mode | Endpoint |
| Specimen | Serum, Heparin Plasma, EDTA Plasma |
| Wavelength | 546 nm |
| Standard Concentration | 4 g/dL |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Incubation | 5 minutes at room temperature |
| Reaction Direction | Increasing |
| Linearity | Up to 12 g/dL |
| Reagent Preparation | Ready to Use |
| Reagent Storage | 15–30°C / Room Temperature |
| Standard Storage | 2–8°C |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The analytical parameters above are taken from the Swemed Total Protein product literature.
For the 2 × 50 ml Swemed Total Protein Kit, the manufacturer lists:
| Component | Quantity |
|---|---|
| Total Protein Reagent | 2 × 50 ml |
| Total Protein Standard | 1 vial |
| Instructions for Use | 1 |
The supplied standard has a concentration of 4 g/dL.
The Swemed Total Protein Reagent Kit can be used with open-system fully automatic and semi-automatic biochemistry analysers that allow user programming of the required assay parameters.
Typical analyser requirements include:
| Analyser Parameter | Swemed Total Protein Setting |
|---|---|
| Method | Endpoint |
| Wavelength | 546 nm |
| Reaction | Increasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Standard | 4 g/dL |
| Incubation | 5 minutes |
| Blank | Reagent Blank |
| Unit | g/dL |
The laboratory should confirm that its analyser supports these parameters and should validate the application according to the analyser manufacturer's instructions and the current Swemed IFU before reporting patient results.
Swemed states that both the reagent and standard are ready to use, helping laboratories reduce reagent-preparation steps during routine testing.
The Biuret method is widely used for routine total protein determination because proteins form a coloured complex with cupric ions in alkaline conditions.
The defined endpoint settings make the reagent practical for laboratories using programmable open-system semi-automatic and fully automatic biochemistry analysers.
Total protein is frequently measured as part of:
The 2 × 50 ml pack is practical for laboratories requiring a compact professional reagent configuration.
The Swemed Total Protein Reagent Kit is intended for the photometric quantitative determination of total protein in serum and plasma using the Biuret Method.
Swemed lists the following suitable specimens:
This product is intended for professional in vitro diagnostic use only.
Proteins contain peptide bonds that react with cupric ions in an alkaline medium.
This reaction forms a blue-violet copper-protein complex.
The intensity of the colour formed is proportional to the concentration of total protein in the specimen.
| Component | Manufacturer Specification |
|---|---|
| Copper Sulphate | 2 g/L |
| Potassium Iodide | 5 g/L |
| Sodium Hydroxide | 8 g/L |
| Total Protein Standard | 4 g/dL |
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | Total Protein |
| Method | Biuret |
| Mode | Endpoint |
| Wavelength | 546 nm |
| Unit | g/dL |
| Temperature | 37°C |
| Standard Concentration | 4 g/dL |
| Reaction Slope | Increasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Incubation Time | 5 minutes at room temperature |
| Blanking | Reagent Blank |
| Linearity | 12 g/dL |
| Tube | Reagent | Standard / Sample |
|---|---|---|
| Blank | 1000 µl | — |
| Standard | 1000 µl | 20 µl Standard |
| Test | 1000 µl | 20 µl Sample |
Mix and incubate for 5 minutes at room temperature, then read absorbance against the reagent blank at 546 nm.
Total Protein (g/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration
The supplied standard concentration is 4 g/dL.
Swemed lists:
Fresh, clear, non-haemolysed serum from fasting patients is recommended.
The manufacturer warns that haemolysis may cause falsely elevated total protein results because released haemoglobin increases measured protein and background colour. Samples containing bromsulfophthalein (BSP) may also produce falsely elevated results.
According to the Swemed product literature:
| Storage Condition | Stability |
|---|---|
| Serum / Plasma at 2–8°C | 6 days |
Samples should be protected from bacterial contamination and evaporation.
The Swemed Total Protein assay is linear up to 12 g/dL.
For results above 12 g/dL, the manufacturer instructs laboratories to dilute the sample 1:1 with 0.9% NaCl solution, repeat the assay and multiply the result by 2.
The manufacturer also states that the Biuret procedure is not sufficiently sensitive at very low protein concentrations below 1 g/dL, and this kit should not be used for urine or cerebrospinal fluid protein testing.
| Specimen | Manufacturer Reference Range |
|---|---|
| Serum / Plasma | 6.2–8.5 g/dL |
Each laboratory should verify whether the manufacturer reference interval is suitable for its own patient population and analyser system.
The manufacturer states:
Signs of possible reagent deterioration include:
Proteins are essential structural and functional components of the body and are involved in:
Changes in total protein concentration may be associated with conditions such as dehydration, impaired protein synthesis, malnutrition, protein loss and increased protein breakdown.
Total protein results should always be interpreted together with the patient's clinical history and related tests.
| Related Parameter | Laboratory Relevance |
|---|---|
| Total Protein | Overall serum protein concentration |
| Albumin | Major circulating plasma protein |
| Globulin | Often calculated from Total Protein – Albumin |
| A/G Ratio | Albumin-to-globulin relationship |
| Bilirubin | Liver-related assessment |
| SGOT / AST | Liver and tissue enzyme |
| SGPT / ALT | Liver-associated enzyme |
| Alkaline Phosphatase | Hepatobiliary / bone-related enzyme |
| Creatinine | Renal-function assessment |
| Urea | Renal and protein-metabolism assessment |
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine total protein testing |
| Diagnostic Centres | Clinical chemistry testing |
| Hospital Laboratories | Inpatient and outpatient investigations |
| Liver / Gastroenterology Centres | Liver-function profiles |
| Nutrition Clinics with Laboratories | Protein-status assessment |
| Medical Institutions | Diagnostic and teaching laboratory use |
| Clinical Chemistry Laboratories | Routine biochemical testing |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies clinical biochemistry reagents, liver-function reagents and laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed Total Protein Reagent Kit 2 × 50 ml is suitable for pathology laboratories, diagnostic centres, hospitals, clinical chemistry laboratories and institutional healthcare buyers.
It can be ordered through microsidd.com, subject to stock availability and delivery serviceability.
This product is relevant for customers searching for:
The Swemed Total Protein Reagent Kit 2 × 50 ml is a ready-to-use professional clinical chemistry reagent used for photometric quantitative determination of total protein using the Biuret Method.
Swemed lists:
The assay is performed at 546 nm.
The supplied Total Protein standard concentration is 4 g/dL.
The manufacturer procedure uses 20 µl sample with 1000 µl reagent.
The stated linearity is 12 g/dL.
Yes. Swemed states that the reagent and standard are ready to use.
It can be used on open-system fully automatic analysers that allow programming of the required endpoint method, wavelength and reagent/sample volumes, subject to analyser compatibility and laboratory validation.
Yes, it is suitable for compatible open-system semi-automatic biochemistry analysers after appropriate programming and validation.
No. The manufacturer states that this Biuret assay is not suitable for low protein concentrations below 1 g/dL and should not be used for urine or spinal fluid testing.
The product can be ordered from Microsidd India through microsidd.com, subject to stock availability and serviceability.
| Entity | Details |
|---|---|
| Product | Total Protein Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Manufacturer Reference | TPR100 |
| Analyte | Total Protein |
| Method | Biuret |
| Test Type | Photometric Endpoint |
| Specimen | Serum / Heparin Plasma / EDTA Plasma |
| Wavelength | 546 nm |
| Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Clinical Category | Liver Function / General Clinical Chemistry |
| Analyser Compatibility | Open-System Fully & Semi Automatic Biochemistry Analysers |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Hospital / Diagnostic Centre / B2B |
Laboratory reagents should be purchased with attention to much more than price. The laboratory needs confidence regarding the correct method, pack size, standard concentration, analyser compatibility, reagent storage, expiry, product source and seller accountability.
Microsidd India has long-standing experience in the IVD and diagnostic-products sector and focuses specifically on laboratory, diagnostic and healthcare products. Professional buyers therefore purchase through an identifiable specialist diagnostic supplier rather than relying only on a general marketplace seller.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic and laboratory focused | Seller expertise varies | Depends on dealer |
| Product Understanding | Better suited to reagent-specific enquiries | Often general marketplace support | Dealer dependent |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and seller may differ | Usually locally identifiable |
| Pack Verification | 2 × 50 ml pack can be checked before ordering | Similar listings may create confusion | Depends on local stock |
| Method Verification | Biuret methodology can be confirmed | Seller dependent | Dealer dependent |
| Analyser Compatibility Guidance | Better suited to open-system reagent queries | Often limited | Depends on dealer knowledge |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for laboratories and institutions | Seller dependent | Usually local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but not specialised | Usually limited |
| Customer Support | Direct Microsidd support | Marketplace plus seller layer | Local support |
| Accountability | One identifiable specialist supplier | Seller may vary between listings | Depends on supplier |
| India-Wide Supply | Subject to serviceability | Broad | Usually regional |
| Microsidd Advantage | Benefit to Laboratory Buyers |
|---|---|
| Diagnostic Product Specialisation | Better understanding of professional laboratory requirements |
| Long-Standing IVD Experience | Familiarity with diagnostic and clinical chemistry products |
| Direct Seller Accountability | Purchase directly from Microsidd India |
| Product Verification | Pack, method and technical specifications can be checked |
| Open-System Analyser Support | Easier verification of programmable reagent parameters |
| GST Invoice | Useful for laboratories and institutional procurement |
| Multi-Category Procurement | Liver, renal, lipid, cardiac and other reagents can be sourced together |
| Bangalore-Based Operations | Convenient for Bengaluru and Karnataka laboratories |
| India-Wide Supply | Delivery to serviceable locations across India |
| Direct Customer Support | Order and product queries handled directly |
It is used for quantitative measurement of total protein in serum and plasma by the Biuret Method.
The pack size is 2 × 50 ml.
It uses the Biuret Method.
The assay is measured at 546 nm.
The standard is 4 g/dL.
Serum, heparin plasma and EDTA plasma.
The assay uses 20 µl sample.
The manufacturer procedure uses 1000 µl reagent.
The assay requires 5 minutes at room temperature.
The stated linearity is 12 g/dL.
Yes. The reagent and standard are ready to use.
Yes, if the analyser is an open system and supports the required endpoint method, 546 nm wavelength and programmable reagent/sample settings.
Yes, for open-system fully automatic analysers that allow reagent application programming. The laboratory must validate the method before routine reporting.
No. Swemed states that the Biuret procedure should not be used for urine or spinal-fluid protein testing because it is not sensitive enough at very low protein levels.
The reagent is stored at 15–30°C, while the standard is stored at 2–8°C. Do not freeze.
The product can be ordered from Microsidd India through microsidd.com, subject to availability.
Yes. Microsidd supplies laboratory and diagnostic products to serviceable locations across India.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
Total protein results should not be interpreted independently to diagnose or exclude liver disease, nutritional disorders, kidney disease or other medical conditions. Results should be correlated with albumin, globulin, liver-function tests, renal-function tests, clinical history and other relevant investigations.
The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.
For open-system fully automatic and semi-automatic analysers, analyser compatibility and application settings must be verified and validated by the laboratory before routine clinical use.
Specimen requirements, reagent preparation, analyser settings, standardisation, reference intervals, storage, stability and interpretation should always follow the current manufacturer instructions and validated laboratory procedures.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Reagent, Laboratory |
| Test method | Lab analysis |
| Test sample | Blood |
