The Swemed Urea Berthelot Reagent Kit 2 × 50 ml is a professional clinical biochemistry reagent intended for the photometric quantitative determination of urea in serum, plasma and urine using the established Berthelot Method.
In this method, urease hydrolyses urea to produce ammonia. The ammonia subsequently reacts with a phenolic chromogen and hypochlorite to form a green-coloured complex. The intensity of the colour produced is directly proportional to the concentration of urea in the specimen.
Swemed's current catalogue lists Urea (Berthelot) in 2 × 50 ml and 2 × 100 ml presentations, with the Berthelot Method and a listed shelf life of 24 months.
The 2 × 50 ml variant is suitable for pathology laboratories, diagnostic centres, hospital laboratories and clinical chemistry facilities using compatible open-system fully automatic or semi-automatic biochemistry analysers.
| Product Detail | Information |
|---|---|
| Product Name | Urea Berthelot Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Product Category | Clinical Biochemistry Reagent |
| Test Parameter | Urea |
| Method | Berthelot Method |
| Measurement | Photometric / Colorimetric |
| Mode | Endpoint |
| Wavelength | 578 nm |
| Temperature | 37°C |
| Standard Concentration | 50 mg/dL |
| Reaction Direction | Increasing |
| Sample Volume | 10 µl |
| Quick-Reference Reagent Volume | 1000 µl |
| Linearity | Up to 300 mg/dL |
| Specimens | Serum, Plasma, Urine |
| Plasma Types | Heparin / EDTA |
| Reagent Preparation | Ready to Use |
| Storage | 2–8°C |
| Analyser Compatibility | Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
Swemed specifies endpoint measurement at 578 nm, a 50 mg/dL standard, 10 µl sample volume and linearity up to 300 mg/dL.
The Swemed Urea Berthelot system includes:
| Component | Description |
|---|---|
| R1 | Buffer Reagent |
| R2 | Enzyme Reagent |
| R3 | Chromogen Reagent |
| Standard | Urea Berthelot Standard |
| IFU | Instructions for Use |
Swemed lists the 2 × 50 ml configuration as reference UB50 in its technical insert.
The assay uses urease followed by the Berthelot colour reaction, providing a conventional enzymatic-colorimetric method for routine urea measurement.
Swemed states that the reagents and standard are ready to use, reducing reagent-preparation steps during routine testing.
Urea is one of the most frequently requested renal biochemistry parameters and is commonly tested alongside:
The reagent can be programmed on compatible open-system fully automatic and semi-automatic analysers that support endpoint photometric testing at 578 nm, subject to application validation by the laboratory.
The manufacturer states linearity up to 300 mg/dL, suitable for routine clinical chemistry applications.
The Swemed Urea Berthelot Reagent is suitable for compatible programmable open-system analysers.
| Analyser Parameter | Swemed Setting |
|---|---|
| Method | Endpoint |
| Wavelength | 578 nm |
| Temperature | 37°C |
| Unit | mg/dL |
| Standard Concentration | 50 mg/dL |
| Reaction Direction | Increasing |
| Sample Volume | 10 µl |
| Reagent Volume | 1000 µl quick-reference setting |
| Blank | Reagent Blank |
| Linearity | 300 mg/dL |
Analyser compatibility must be confirmed and the reagent application validated by the laboratory before reporting patient results.
The Swemed Urea Berthelot Reagent Kit is intended for the photometric quantitative determination of urea in serum, plasma and urine.
Swemed specifically lists:
The product is intended for professional in vitro diagnostic use only.
Urease first hydrolyses urea:
Urea + H₂O → Ammonia + CO₂
The ammonia produced subsequently reacts with a phenolic chromogen and hypochlorite, producing a green-coloured complex.
The intensity of the colour formed is directly proportional to the urea concentration in the specimen.
| Parameter | Manufacturer Specification |
|---|---|
| Test | Urea Berthelot |
| Method | Berthelot |
| Mode | Endpoint |
| Wavelength | 578 nm |
| Temperature | 37°C |
| Unit | mg/dL |
| Standard | 50 mg/dL |
| Reaction Slope | Increasing |
| Quick-Reference Reagent Volume | 1000 µl |
| Sample Volume | 10 µl |
| Blanking | Reagent Blank |
| Linearity | 300 mg/dL |
For the manual procedure, Swemed specifies:
| Component | Blank | Standard | Test |
|---|---|---|---|
| R1 | 500 µl | 500 µl | 500 µl |
| R2 | 50 µl | 50 µl | 50 µl |
| Standard | — | 10 µl | — |
| Sample | — | — | 10 µl |
Mix and incubate:
5 minutes at 37°C
or
10 minutes at room temperature
Then add:
| Component | Blank | Standard | Test |
|---|---|---|---|
| R3 | 500 µl | 500 µl | 500 µl |
Mix again and incubate for 5 minutes at 37°C or 10 minutes at room temperature, then measure absorbance against the blank at 578 nm.
Urea (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration
The manufacturer standard concentration is:
50 mg/dL
Swemed identifies the following acceptable specimens:
The manufacturer recommends using non-haemolysed serum or plasma.
Acceptable anticoagulants listed in the insert include:
Ammonium heparin should not be used, because ammonium ions may falsely increase the result.
The Swemed Urea Berthelot reagent can also be used for urine urea determination.
According to the manufacturer, urine should be:
Pre-diluted 1:50 using ammonium-free water before testing.
| Storage Condition | Stability |
|---|---|
| 15–25°C | 7 days |
| 2–8°C | 7 days |
| −20°C | 1 year |
| Storage Condition | Stability |
|---|---|
| 15–25°C | 2 days |
| 2–8°C | 7 days |
| −20°C | 1 month |
Swemed states that the Berthelot reaction is linear up to:
300 mg/dL
Samples above the linearity limit should be diluted 1 + 2 with physiological saline, repeated and the obtained result multiplied by 3.
The manufacturer notes:
Proper sample collection and laboratory handling are therefore important.
| Specimen | Swemed Reference |
|---|---|
| Serum / Plasma | 10–50 mg/dL |
| Urine – 24 hours | 20–35 g/24 h |
Reference intervals can vary between laboratories and populations. Each laboratory should establish or verify its own appropriate reference interval.
Swemed states that the reagent and standard should be:
Signs of reagent deterioration include:
The Swemed catalogue lists a 24-month shelf life for the Urea Berthelot product when properly stored.
Urea is an end product of protein metabolism. It is formed primarily in the liver from ammonia generated during amino-acid metabolism and is transported in the bloodstream to the kidneys for excretion.
Urea testing commonly forms part of:
Urea values should not be interpreted independently because concentration can be affected by renal function, hydration, dietary protein intake, liver function and other clinical factors.
| Test | Laboratory Relevance |
|---|---|
| Urea | Protein metabolism and renal excretion |
| Creatinine | Renal filtration assessment |
| Uric Acid | Purine metabolism |
| Sodium | Electrolyte and fluid balance |
| Potassium | Electrolyte and renal assessment |
| Chloride | Electrolyte / acid-base assessment |
| Calcium | Mineral metabolism |
| Phosphorus | Renal and mineral metabolism |
| Feature | Urea Berthelot | Urea UV |
|---|---|---|
| Method | Berthelot | GLDH |
| Measurement | Colorimetric Endpoint | UV Fixed-Time / Kinetic |
| Typical Wavelength | 578 nm | 340 nm |
| Swemed Berthelot Pack | 2 × 50 ml / 2 × 100 ml | Different pack configuration |
| Application | Routine colorimetric urea testing | UV-based analyser testing |
| Selection | Depends on analyser and laboratory method | Depends on analyser and laboratory method |
Swemed lists Berthelot and Urea UV as separate reagent products; customers should verify the required method before ordering.
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine urea testing |
| Diagnostic Centres | Renal profile testing |
| Hospital Laboratories | Inpatient and outpatient chemistry |
| Nephrology Centres | Renal-function investigations |
| Dialysis Centres | Renal biochemical assessment |
| Clinical Chemistry Laboratories | Routine urea estimation |
| Medical Institutions | Teaching and diagnostic laboratory use |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies renal-profile reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed Urea Berthelot Reagent Kit 2 × 50 ml is suitable for pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and professional clinical chemistry facilities.
The product can be ordered through microsidd.com, subject to stock availability and delivery serviceability.
This product is relevant for buyers searching for:
The Swemed Urea Berthelot Reagent Kit 2 × 50 ml is a professional clinical chemistry reagent used for quantitative photometric measurement of urea using the Berthelot Method.
This Shopify listing is for:
2 × 50 ml
Swemed also lists a 2 × 100 ml variant.
It uses the Berthelot Method.
The assay is measured at:
578 nm
The manufacturer specifies:
10 µl
50 mg/dL
Up to 300 mg/dL
Yes, for compatible open-system semi-automatic biochemistry analysers after programming and laboratory validation.
Yes, for compatible open-system fully automatic analysers that support the required endpoint application and permit third-party reagent programming.
The product can be ordered from Microsidd India through microsidd.com, subject to stock availability.
| Entity | Details |
|---|---|
| Product | Urea Berthelot Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Test Parameter | Urea |
| Method | Berthelot |
| Measurement | Colorimetric Endpoint |
| Wavelength | 578 nm |
| Specimen | Serum / Plasma / Urine |
| Clinical Category | Renal Profile |
| Product Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Analyser Compatibility | Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Hospital / Diagnostic Centre / B2B |
Clinical chemistry reagents require careful verification of the parameter, methodology, pack size, analyser compatibility, storage requirements, expiry and seller source before purchase.
Microsidd India has long-standing experience in the IVD and diagnostic-products sector and focuses on laboratory, diagnostic and healthcare products. This gives professional laboratories an identifiable specialist procurement source rather than relying exclusively on general marketplace listings.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic & laboratory focused | Seller expertise varies | Dealer dependent |
| Product Understanding | Suitable for reagent-specific enquiries | Often general support | Depends on supplier |
| Seller Identity | Direct Microsidd India purchase | Marketplace and seller may differ | Usually identifiable |
| Method Verification | Berthelot vs Urea UV can be checked | Similar listings may confuse buyers | Dealer dependent |
| Pack Verification | 2 × 50 ml can be confirmed | Listing accuracy varies | Depends on local stock |
| Open-System Guidance | Better suited to analyser-setting enquiries | Often limited | Dealer dependent |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for labs and institutions | Seller dependent | Usually local |
| Product Range | Specialist laboratory catalogue | Broad but not specialised | Usually limited |
| Customer Support | Direct Microsidd support | Marketplace + seller layer | Local support |
| Seller Accountability | Single identifiable specialist seller | Seller may vary | Supplier dependent |
| India-Wide Supply | Subject to serviceability | Broad | Usually regional |
It is used for quantitative determination of urea in serum, plasma and urine using the Berthelot Method.
2 × 50 ml
No. Berthelot is a colorimetric endpoint method, whereas Urea UV uses a GLDH-based UV method.
578 nm
10 µl
50 mg/dL
The assay is linear up to 300 mg/dL.
Yes. Swemed states that the reagent and standard are ready to use.
Yes. Swemed instructs laboratories to pre-dilute urine 1:50 with ammonium-free water before assay.
No. Ammonium heparin should not be used because ammonium ions may produce falsely elevated values.
At 2–8°C, protected from light and contamination. Do not freeze.
Yes, when the analyser is an open system and supports the required endpoint settings. The application should be validated before routine use.
Yes, on compatible open-system fully automatic biochemistry analysers that permit the required reagent programming.
No. It is for professional in vitro diagnostic use only.
It can be purchased from Microsidd India through microsidd.com, subject to availability.
The information on this product page is provided for general product identification, procurement and laboratory educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
Urea results should not be interpreted independently to diagnose or exclude kidney disease or any other medical condition. Clinical interpretation may require creatinine, electrolytes, urine investigations, eGFR, patient history and other relevant investigations.
The information provided by Microsidd India does not replace the current Swemed Diagnostics product insert, analyser instructions, laboratory SOPs, calibration requirements, quality-control procedures or professional medical judgement.
For open-system fully automatic and semi-automatic analysers, compatibility, reagent programming and application performance must be verified and validated by the laboratory before routine clinical reporting.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Reagent, Laboratory |
