The SWEMED Alkaline Phosphatase Reagent Kit – 25 mL is a professional clinical biochemistry reagent intended for the quantitative determination of alkaline phosphatase (ALP) activity in human serum using the PNPP kinetic method.
The 25 mL kit is supplied as 20 mL of Buffer Reagent (R1) + 5 mL of Substrate Reagent (R2) and is designed for routine use in clinical laboratories, hospitals, diagnostic centres and pathology laboratories.
Product Name: Alkaline Phosphatase Reagent Kit
Brand: SWEMED
Manufacturer: SWEMED Biomedicals Pvt. Ltd.
Product Category: Laboratory Reagent / Clinical Chemistry Reagent
Subcategory: Biochemistry Reagent
Test Parameter: Alkaline Phosphatase (ALP)
Methodology: PNPP Kinetic Method
Product Code: ALP 702
Pack Reference: ALKP25
Pack Size: 25 mL
Pack Configuration: R1 20 mL + R2 5 mL
Sample Type: Serum
Measurement Type: Quantitative ALP Activity Determination
Reagent Type: Two-Reagent System
Intended Use: Professional in-vitro diagnostic laboratory testing
Suitable Users: Diagnostic laboratories, hospitals, pathology laboratories and clinical chemistry laboratories
Storage: 2–8°C for reagent kit, protect from light
Shelf Life: Manufacturer catalogue states 24 months
Seller: Microsidd India
The SWEMED Alkaline Phosphatase Reagent Kit is an in-vitro diagnostic reagent used for the quantitative measurement of alkaline phosphatase enzyme activity in human serum.
Alkaline phosphatase is an enzyme found in several tissues, with important concentrations in the liver, biliary tract and bone. Measurement of ALP is commonly included in routine clinical chemistry testing, especially in the assessment of hepatobiliary and bone-related conditions.
The SWEMED assay uses the PNPP kinetic method, in which alkaline phosphatase hydrolyses p-nitrophenyl phosphate (PNPP) under alkaline conditions to form p-nitrophenol. The increase in absorbance is proportional to the ALP activity in the sample.
The SWEMED Alkaline Phosphatase Reagent Kit is intended for:
ALP results should be interpreted alongside other clinical information and related laboratory parameters.
Uses a PNPP kinetic assay for quantitative determination of alkaline phosphatase activity.
The 25 mL kit contains:
R1 Buffer Reagent: 1 × 20 mL
R2 Substrate Reagent: 1 × 5 mL
Designed for use in professional laboratory environments performing routine biochemistry testing.
The working reagent is prepared by mixing 4 parts of R1 with 1 part of R2, according to the manufacturer’s IFU.
SWEMED states working-reagent stability of approximately:
5 days at 15–25°C
2 weeks at 2–8°C
SWEMED’s current catalogue lists 24 months shelf life for the Alkaline Phosphatase reagent kit.
The assay is based on the enzymatic hydrolysis of p-nitrophenyl phosphate (PNPP).
Under alkaline conditions:
PNPP + H₂O → p-Nitrophenol + Phosphate
This reaction is catalysed by alkaline phosphatase present in the patient sample.
The rate at which p-nitrophenol forms is measured by the analyser as an increase in absorbance. The rate of absorbance change is proportional to the ALP activity in the serum specimen.
This reagent is intended for trained laboratory professionals working in:
It is not intended for consumer home testing.
ALKP-R1 Buffer Reagent: 1 × 20 mL
ALKP-R2 Substrate Reagent: 1 × 5 mL
Instructions for Use: 1
Total reagent volume: 25 mL.
The reagent should be used according to the current SWEMED Instructions for Use and the operating requirements of the compatible biochemistry analyser.
The manufacturer specifies preparation of working reagent by mixing:
4 parts R1 + 1 part R2.
For routine laboratory use:
| Information | Details |
|---|---|
| Brand | SWEMED |
| Product | Alkaline Phosphatase Reagent Kit |
| Parameter | ALP |
| Method | PNPP Kinetic |
| Product Code | ALP 702 |
| Pack Reference | ALKP25 |
| Pack Size | 25 mL |
| R1 | 20 mL |
| R2 | 5 mL |
| Sample | Serum |
| Reagent System | Two-Reagent |
| Storage | 2–8°C |
| Shelf Life | 24 months, manufacturer-listed |
| Use | Professional IVD |
| Specification | Details |
|---|---|
| Product Name | Alkaline Phosphatase Reagent Kit |
| Brand | SWEMED |
| Manufacturer | SWEMED Biomedicals Pvt. Ltd. |
| Analyte | Alkaline Phosphatase |
| Methodology | PNPP Kinetic Method |
| Product Code | ALP 702 |
| Pack Reference | ALKP25 |
| Pack Configuration | R1 20 mL + R2 5 mL |
| Total Volume | 25 mL |
| Specimen | Serum |
| Measurement Type | Quantitative Enzyme Activity |
| Reagent Preparation | 4 parts R1 + 1 part R2 |
| Storage | 2–8°C |
| Working Reagent Stability | 5 days at 15–25°C / 2 weeks at 2–8°C |
| Product Type | Clinical Biochemistry IVD Reagent |
SWEMED specifies that the reagents are stable until the labelled expiry date when stored at 2–8°C, protected from light and contamination. The substrate reagent is particularly light- and temperature-sensitive.
For proper handling:
https://microsidd.com/collections/chemicals-liquids
https://microsidd.com/collections/bio-chemistry-reagents
SWEMED Albumin Reagent Kit 2 × 50 mL https://microsidd.com/products/swemed-albumin-reagent-kit-2x50ml
✓ SWEMED clinical biochemistry reagent
✓ PNPP kinetic methodology
✓ 25 mL professional laboratory pack
✓ R1 + R2 reagent system
✓ Suitable for routine ALP testing
✓ Designed for professional clinical chemistry laboratories
✓ Related SWEMED reagents available
✓ Microsidd India – Bengaluru
✓ Delivery to serviceable locations across India
It is a professional clinical chemistry reagent for the quantitative determination of alkaline phosphatase activity in human serum.
It uses the PNPP Kinetic Method.
The pack contains 25 mL total reagent.
It contains 20 mL R1 Buffer Reagent + 5 mL R2 Substrate Reagent.
SWEMED’s current catalogue lists ALP 702.
The IFU identifies the 25 mL configuration as ALKP25.
SWEMED’s IFU specifies serum.
The IFU specifically warns that EDTA, oxalate and fluoride inhibit alkaline phosphatase and are unsuitable anticoagulants for this assay.
Mix 4 parts R1 with 1 part R2.
SWEMED states approximately 5 days at 15–25°C and 2 weeks at 2–8°C.
Store at 2–8°C, protected from light and contamination. Do not freeze.
The current SWEMED catalogue states 24 months.
No. It is intended for professional in-vitro diagnostic laboratory use.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
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The SWEMED Alkaline Phosphatase Reagent Kit is intended for professional in-vitro diagnostic use.
Testing should be performed by trained laboratory personnel according to the manufacturer’s current Instructions for Use, compatible analyser specifications, laboratory SOPs and quality-control procedures.
ALP results should not be interpreted in isolation and should be considered together with other laboratory findings and appropriate clinical assessment.
| Specification name | Specification Value |
|---|---|
| Color pattern | White |
