The Swemed SGOT (AST) Liquid Stable Reagent Kit 50 ml is a professional clinical biochemistry reagent intended for quantitative determination of Aspartate Aminotransferase (AST), also known as SGOT, using the Modified IFCC kinetic method.
Swemed’s official SGOT literature describes the assay as a kinetic UV method in which AST catalyses transfer of an amino group from L-aspartate to α-ketoglutarate, producing oxaloacetate and L-glutamate. The oxaloacetate then reacts with NADH in the presence of malate dehydrogenase, and the resulting decrease in absorbance at 340 nm is proportional to SGOT/AST activity in the sample.
SGOT/AST is an important enzyme found in tissues including the liver, heart, skeletal muscle and kidneys. Its measurement is commonly included in liver function testing and broader clinical chemistry enzyme panels.
This product is intended for professional in vitro diagnostic use only in pathology laboratories, diagnostic centres, hospitals and clinical chemistry laboratories.
| Product Detail | Information |
|---|---|
| Product Name | SGOT / AST Liquid Stable Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 50 ml |
| Product Type | Clinical Biochemistry Reagent |
| Test Parameter | SGOT / AST |
| Full Name | Aspartate Aminotransferase |
| Method | Modified IFCC |
| Measurement Mode | Kinetic UV |
| Reaction Direction | Decreasing |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Specimen | Serum |
| Swemed Website Listing | Serum / Plasma (EDTA / Heparin) |
| Reagent System | R1 + R2 |
| Working Reagent Ratio | 4 parts R1 + 1 part R2 |
| Reagent Volume | 1000 µl |
| Sample Volume | 100 µl |
| Delay Time | 60 seconds |
| Reaction Time | 180 seconds |
| Reading Interval | 60 seconds |
| Calculation Factor | 1746 |
| Linearity | Up to 300 U/L |
| Storage | 2–8°C |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The technical assay parameters above are taken from Swemed’s official SGOT product insert.
The Swemed SGOT assay uses a Modified IFCC kinetic method, providing a standardised enzymatic approach for routine AST activity measurement.
The product uses a two-reagent system designed for routine laboratory use and working-reagent preparation.
The manufacturer states that prepared working reagent remains stable for:
SGOT / AST is commonly measured together with SGPT / ALT, bilirubin, alkaline phosphatase, albumin and total protein as part of liver function assessment.
The method is suitable for trained laboratory personnel using compatible semi-automated or automated chemistry systems after analyser validation.
The Swemed SGOT / AST Reagent Kit is intended for quantitative determination of Aspartate Aminotransferase activity in professional clinical chemistry laboratories.
The official Swemed insert specifies serum as the specimen and recommends fresh, clear, non-haemolysed serum. Swemed’s biochemistry listing also associates SGOT with serum/plasma including EDTA and heparin plasma, so the actual specimen type should follow the current kit insert supplied with the product and the laboratory’s validated method.
SGOT / AST catalyses the following reaction:
L-Aspartate + α-Ketoglutarate → L-Glutamate + Oxaloacetate
The oxaloacetate formed then reacts with NADH in the presence of malate dehydrogenase (MDH):
Oxaloacetate + NADH + H+ → L-Malate + NAD+
The rate at which NADH is oxidised to NAD+ is measured as a decrease in absorbance at 340 nm, and this rate is proportional to AST activity in the sample.
| Component | Manufacturer Specification |
|---|---|
| Reagent 1 | Tris Buffer pH 7.8 – 6 g/L |
| Reagent 1 | L-Aspartate – 30.5 g/L |
| Reagent 1 | LDH – 4 kU/L |
| Reagent 1 | MDH – 700 U/L |
| Reagent 2 | α-Ketoglutarate – 3.4 g/L |
| Reagent 2 | NADH – 1.1 g/L |
The manufacturer also states that the reagents contain sodium azide 0.95 g/L as preservative.
Prepare the working reagent by mixing:
4 parts Reagent R1 + 1 part Reagent R2
Alternatively, the Swemed insert states that 0.8 ml R1 + 0.2 ml R2 may be used directly instead of 1 ml of pre-mixed working reagent during the assay.
The working reagent should have an absorbance above 1.0 against distilled water at 340 nm. Reagent falling below this level should not be used.
| Storage Condition | Stability |
|---|---|
| 15–25°C | 7 days |
| 2–8°C | 4 weeks |
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | SGOT / AST |
| Method | Modified IFCC |
| Mode | Kinetic |
| Wavelength | 340 nm |
| Unit | IU/L |
| Temperature | 37°C |
| Reaction Slope | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 100 µl |
| Delay Time | 60 seconds |
| Reaction Time | 180 seconds |
| Reading Interval | 60 seconds |
| Blanking | Water Blank |
| Factor | 1746 |
| Linearity | 300 U/L |
These values are listed in Swemed’s quick-reference section.
| Component | Macro Method | Semi-Micro Method |
|---|---|---|
| Reagent R1 + R2 | 800 µl + 200 µl | 400 µl + 100 µl |
| Sample | 100 µl | 50 µl |
Mix well and read the initial absorbance after 1 minute. Continue readings at approximately 1-minute intervals for 3 minutes, then calculate the mean absorbance change per minute.
SGOT / AST (U/L) = ΔA/min × 1746
The manufacturer states a linearity of 300 U/L.
When the reaction rate exceeds the stated measuring range, Swemed describes dilution of the sample 1 + 9 with 0.9% NaCl, followed by multiplication of the obtained result by 10.
The official product insert recommends:
This is particularly important because red blood cells contain AST, and haemolysis may cause falsely elevated results.
According to the Swemed product literature, SGOT / AST in serum is reported stable for:
| Storage Condition | Stability |
|---|---|
| 15–30°C | 4 days |
| 2–8°C | 10 days |
| −20°C | 2 weeks |
The manufacturer reports that under the evaluated conditions:
had negligible effect on the assay.
However, haemolysed samples should still be avoided because red cells contain AST and can produce misleadingly elevated results.
| Group | Manufacturer Reference |
|---|---|
| Women | < 31 U/L |
| Men | < 32 U/L |
Swemed also advises that each laboratory establish or validate reference intervals appropriate for its own patient population.
AST is present in several tissues, particularly:
AST activity may rise when cells in these tissues are injured. SGOT / AST testing may therefore form part of laboratory investigations related to:
Because AST is not liver-specific, results should be interpreted together with other parameters such as ALT / SGPT, bilirubin, alkaline phosphatase, albumin and clinical findings.
| Test | Laboratory Relevance |
|---|---|
| SGOT / AST | Hepatic and extra-hepatic enzyme assessment |
| SGPT / ALT | More liver-associated transaminase |
| Bilirubin Total | Bilirubin metabolism |
| Bilirubin Direct | Conjugated bilirubin assessment |
| Alkaline Phosphatase | Hepatobiliary and bone-related enzyme |
| Albumin | Liver synthetic function / nutritional assessment |
| Total Protein | Protein status |
| GGT | Hepatobiliary enzyme where included |
Swemed states that the reagents should be:
The manufacturer also advises against using reagent if the initial absorbance at 340 nm falls below the stated acceptable level or if performance requirements are not met.
Laboratory personnel should:
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine AST / SGOT testing |
| Diagnostic Centres | Liver function testing |
| Hospital Laboratories | Clinical chemistry investigations |
| Gastroenterology Centres | Liver enzyme assessment |
| Hepatology Centres | Liver function monitoring |
| Clinical Chemistry Laboratories | Enzyme testing |
| Medical Institutions | Diagnostic and teaching laboratories |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies liver-function reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed SGOT / AST Liquid Stable Reagent Kit 50 ml is suitable for procurement by pathology laboratories, diagnostic centres, hospitals, gastroenterology centres, clinical chemistry laboratories and other professional healthcare buyers.
Customers can order through microsidd.com, subject to stock availability and delivery serviceability.
This product is relevant for buyers searching for:
The Swemed SGOT / AST Liquid Stable Reagent Kit 50 ml is a professional clinical chemistry reagent used for quantitative measurement of Aspartate Aminotransferase activity using a Modified IFCC kinetic UV method.
The assay is measured at 340 nm.
The macro method uses 100 µl sample, while the semi-micro method uses 50 µl.
The stated factor is 1746.
The manufacturer states a linearity of 300 U/L.
Mix 4 parts R1 with 1 part R2.
Swemed states 7 days at 15–25°C and 4 weeks at 2–8°C.
It can be purchased from Microsidd India through microsidd.com, subject to stock availability.
| Entity | Details |
|---|---|
| Product | SGOT / AST Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 50 ml |
| Analyte | Aspartate Aminotransferase |
| Common Name | SGOT / AST |
| Method | Modified IFCC |
| Measurement | Kinetic UV |
| Wavelength | 340 nm |
| Clinical Category | Liver Function / Clinical Chemistry |
| Product Category | Laboratory Reagents |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Hospital / Diagnostic Centre / B2B |
Clinical chemistry reagents should be purchased with careful attention to the correct analyte, method, reagent configuration, pack size, analyser compatibility, storage requirements, expiry and seller credibility.
Microsidd India has been involved in the IVD and diagnostic-product sector since 2000 and focuses on laboratory, diagnostic and healthcare supplies. Professional buyers therefore deal with an identifiable diagnostic-products specialist rather than depending only on general marketplace sellers.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic and laboratory focused | Seller expertise varies | Depends on dealer |
| Product Knowledge | Better suited to reagent-specific enquiries | Often general marketplace support | Dealer dependent |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and actual seller may differ | Usually locally identifiable |
| Method Verification | SGOT, Modified IFCC and pack can be verified before order | Similar listings may cause confusion | Depends on dealer knowledge |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for labs, hospitals and institutions | Seller dependent | Usually local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but not specialised | Usually limited by local inventory |
| Customer Support | Direct Microsidd assistance | Marketplace plus seller layer | Local support |
| Accountability | One identifiable specialist supplier | Seller may vary between listings | Depends on supplier |
| India-Wide Supply | Subject to serviceability | Broad | Usually regional |
| Microsidd Advantage | Benefit to Laboratory Buyers |
|---|---|
| Diagnostic Product Specialisation | Better suited to professional laboratory procurement |
| IVD Experience Since 2000 | Long-standing diagnostic-sector experience |
| Direct Seller Accountability | Purchase directly from Microsidd India |
| GST Invoice | Useful for laboratories and institutional buyers |
| Product Verification Support | Method, pack and specifications can be checked before purchase |
| Multi-Category Procurement | Liver, renal, lipid, cardiac and other reagents can be sourced together |
| Bangalore-Based Operations | Convenient for Bengaluru and Karnataka laboratories |
| India-Wide Supply | Delivery to serviceable locations across India |
| Direct Customer Support | Product and order queries handled directly by Microsidd |
It is intended for quantitative determination of AST / SGOT enzyme activity in professional clinical chemistry laboratories.
The pack size is 50 ml.
It uses a Modified IFCC kinetic method.
The assay uses 340 nm.
The assay is performed at 37°C.
The macro method uses 100 µl, and the semi-micro method uses 50 µl.
The manufacturer factor is 1746.
The manufacturer states 300 U/L.
Mix 4 parts R1 with 1 part R2.
The manufacturer states 7 days at 15–25°C and 4 weeks at 2–8°C.
It should be avoided because red blood cells contain AST and can produce falsely elevated results.
No. It is intended for professional in vitro diagnostic use only.
The laboratory should verify 340 nm capability, kinetic programming, reagent ratio, sample volume and validated application before ordering.
It can be ordered through Microsidd India at microsidd.com, subject to stock availability.
Microsidd India specialises in diagnostic and laboratory products and provides direct seller accountability, GST invoicing for eligible purchases, product-verification support and professional procurement convenience.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
AST / SGOT is not specific to a single organ. An elevated result should not be used independently to diagnose liver, cardiac, muscular or renal disease. Clinical interpretation should consider ALT / SGPT, bilirubin, alkaline phosphatase, symptoms, history and other relevant investigations.
The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.
Specimen requirements, reagent preparation, analyser settings, calibration, reference intervals, storage, stability and interpretation must always follow current manufacturer instructions and validated laboratory procedures.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Reagent, Laboratory |
| Test method | Lab analysis |
| Test sample | Blood |
