The Swemed SGPT (ALT) Liquid Stable Reagent Kit 50 ml is a professional clinical biochemistry reagent intended for the quantitative determination of Alanine Aminotransferase (ALT), also known as SGPT, using the Modified IFCC kinetic method.
Swemed’s official product literature describes the assay as a kinetic UV method in which ALT catalyses transfer of an amino group from L-alanine to α-ketoglutarate, forming pyruvate and L-glutamate. The pyruvate is then reduced by lactate dehydrogenase with simultaneous oxidation of NADH to NAD+, and the resulting decrease in absorbance at 340 nm is proportional to ALT activity.
SGPT / ALT is one of the most commonly used enzymes in liver function testing and is generally considered more liver-associated than SGOT / AST.
| Product Detail | Information |
|---|---|
| Product Name | SGPT / ALT Liquid Stable Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 50 ml |
| Reagent R1 | 40 ml |
| Reagent R2 | 10 ml |
| Product Type | Clinical Biochemistry Reagent |
| Test Parameter | SGPT / ALT |
| Full Name | Alanine Aminotransferase |
| Method | Modified IFCC |
| Measurement Mode | Kinetic UV |
| Reaction Direction | Decreasing |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Specimen | Serum / Plasma |
| Working Reagent Ratio | 4 parts R1 + 1 part R2 |
| Reagent Volume | 1000 µl |
| Sample Volume | 100 µl |
| Delay Time | 60 seconds |
| Reaction Time | 180 seconds |
| Reading Interval | 60 seconds |
| Calculation Factor | 1746 |
| Linearity | Up to 300 U/L |
| Storage | 2–8°C |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The assay parameters above are taken from the Swemed SGPT product insert; the 50 ml pack configuration with R1 40 ml + R2 10 ml is the pack you are listing on Shopify.
The Swemed SGPT assay uses a Modified IFCC kinetic method, providing a standardised enzymatic approach for routine ALT activity measurement.
The 50 ml kit contains R1 40 ml + R2 10 ml, giving the exact 4:1 reagent ratio required for working-reagent preparation.
The manufacturer states that prepared working reagent remains stable for:
ALT / SGPT is commonly measured together with:
The 40 ml R1 + 10 ml R2 configuration provides a compact pack for laboratories with routine or moderate SGPT testing volumes.
The Swemed SGPT / ALT Liquid Stable Reagent Kit is intended for quantitative determination of Alanine Aminotransferase activity in serum/plasma using a Modified IFCC kinetic UV method.
This product is intended for professional in vitro diagnostic use only.
ALT catalyses:
L-Alanine + α-Ketoglutarate → L-Glutamate + Pyruvate
The pyruvate formed is then reduced by lactate dehydrogenase:
Pyruvate + NADH + H+ → L-Lactate + NAD+
The resulting decrease in NADH absorbance at 340 nm is directly proportional to SGPT / ALT activity in the sample.
| Component | Manufacturer Specification |
|---|---|
| Reagent 1 | Tris Buffer pH 7.8 – 5 g/L |
| Reagent 1 | L-Alanine – 37.5 g/L |
| Reagent 1 | LDH – 4 kU/L |
| Reagent 2 | α-Ketoglutarate – 3.4 g/L |
| Reagent 2 | NADH – 1.1 g/L |
Prepare the working reagent by mixing:
4 parts Reagent R1 + 1 part Reagent R2
For this 50 ml kit:
R1 40 ml + R2 10 ml
Swemed also states that 0.8 ml R1 + 0.2 ml R2 may be used directly instead of 1 ml pre-mixed working reagent during the assay.
The working reagent should have an absorbance above 1.0 against distilled water at 340 nm.
| Storage Condition | Stability |
|---|---|
| 15–25°C | 7 days |
| 2–8°C | 4 weeks |
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | SGPT / ALT |
| Method | Modified IFCC |
| Mode | Kinetic |
| Wavelength | 340 nm |
| Unit | IU/L |
| Temperature | 37°C |
| Reaction Slope | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 100 µl |
| Delay Time | 60 seconds |
| Reaction Time | 180 seconds |
| Reading Interval | 60 seconds |
| Blanking | Water Blank |
| Factor | 1746 |
| Linearity | 300 U/L |
| Component | Macro Method | Semi-Micro Method |
|---|---|---|
| R1 + R2 | 800 µl + 200 µl | 400 µl + 100 µl |
| Sample | 100 µl | 50 µl |
Mix well, read initial absorbance after 1 minute, then read at 1-minute intervals for 3 minutes. Calculate the mean absorbance change per minute.
SGPT / ALT (U/L) = ΔA/min × 1746
The assay is linear up to 300 U/L.
If activity exceeds the stated range, Swemed recommends dilution 1 + 9 with 0.9% NaCl and multiplication of the obtained result by 10.
The manufacturer lists:
Fresh, clear, non-haemolysed serum from fasting patients is recommended. The insert also advises against using blood-collection devices lubricated with glycerin.
According to the Swemed insert:
| Storage Condition | Stability |
|---|---|
| 15–30°C | 3 days |
| 2–8°C | 7 days |
| −20°C | 30 days |
The manufacturer reports negligible interference under the evaluated conditions from:
| Group | Manufacturer Reference |
|---|---|
| Women | < 42 U/L |
| Men | < 32 U/L |
Each laboratory should establish or validate its own reference interval according to its patient population and analyser system.
ALT is present in several tissues but is found in especially high concentrations in the liver and kidneys, and Swemed notes that ALT is generally considered more liver-specific than AST.
SGPT / ALT testing may form part of investigations related to:
ALT results should always be interpreted with clinical findings and related liver-function parameters.
| Test | Laboratory Relevance |
|---|---|
| SGPT / ALT | Liver-associated transaminase |
| SGOT / AST | Hepatic and extra-hepatic transaminase |
| Bilirubin Total | Bilirubin metabolism |
| Bilirubin Direct | Conjugated bilirubin assessment |
| Alkaline Phosphatase | Hepatobiliary and bone-associated enzyme |
| Albumin | Liver synthetic function |
| Total Protein | Protein status |
| GGT | Hepatobiliary enzyme where included |
Store the reagents:
Do not use reagent if the initial absorbance at 340 nm falls below 0.800, or if the reagent fails to meet stated performance criteria.
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine ALT / SGPT testing |
| Diagnostic Centres | Liver function investigations |
| Hospital Laboratories | Clinical chemistry testing |
| Gastroenterology Centres | Liver enzyme assessment |
| Hepatology Centres | Liver function monitoring |
| Clinical Chemistry Laboratories | Routine enzyme testing |
| Medical Institutions | Diagnostic and teaching laboratories |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies liver-function reagents, clinical chemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed SGPT / ALT Liquid Stable Reagent Kit 50 ml with R1 40 ml + R2 10 ml is suitable for pathology laboratories, diagnostic centres, hospitals, liver clinics and professional clinical chemistry laboratories.
Customers can order through microsidd.com, subject to stock availability and serviceability.
This product is relevant for buyers searching for:
The Swemed SGPT / ALT Liquid Stable Reagent Kit 50 ml is a professional clinical chemistry reagent used for quantitative ALT activity measurement by a Modified IFCC kinetic UV method.
The assay uses 340 nm.
The macro method uses 100 µl sample; the semi-micro method uses 50 µl.
The factor is 1746.
The stated linearity is 300 U/L.
Mix 4 parts R1 with 1 part R2.
It can be ordered from Microsidd India through microsidd.com, subject to availability.
| Entity | Details |
|---|---|
| Product | SGPT / ALT Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 50 ml |
| R1 | 40 ml |
| R2 | 10 ml |
| Analyte | Alanine Aminotransferase |
| Common Name | SGPT / ALT |
| Method | Modified IFCC |
| Measurement | Kinetic UV |
| Wavelength | 340 nm |
| Clinical Category | Liver Function / Clinical Chemistry |
| Product Category | Laboratory Reagents |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Hospital / Diagnostic Centre / B2B |
Clinical chemistry reagents should be purchased with careful attention to method, pack configuration, analyser compatibility, storage requirements, expiry and seller credibility.
Microsidd India has long-standing experience in the IVD and diagnostic-products sector and focuses on laboratory, diagnostic and healthcare products rather than operating as a general-purpose marketplace. Professional buyers therefore deal with an identifiable specialist seller for recurring laboratory procurement.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic and laboratory focused | Seller expertise varies | Depends on dealer |
| Product Knowledge | Better suited to reagent-specific queries | Often general marketplace support | Dealer dependent |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and seller may differ | Usually locally identifiable |
| Pack Verification | 50 ml, R1 40 ml + R2 10 ml can be verified before order | Similar listings may cause confusion | Depends on dealer knowledge |
| Method Verification | Modified IFCC can be confirmed | Seller dependent | Dealer dependent |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for labs, hospitals and institutions | Seller dependent | Usually local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but not specialised | Usually limited by local stock |
| Customer Support | Direct Microsidd support | Marketplace plus seller layer | Local support |
| Seller Accountability | One identifiable specialist supplier | Seller may change by listing | Depends on supplier |
| India-Wide Supply | Subject to serviceability | Broad | Usually regional |
| Microsidd Advantage | Benefit to Laboratory Buyers |
|---|---|
| Diagnostic Product Specialisation | Better suited to professional laboratory procurement |
| IVD Sector Experience | Greater familiarity with diagnostic products |
| Direct Seller Accountability | Purchase directly from Microsidd India |
| GST Invoice | Useful for professional buyers |
| Product Verification Support | Method and exact pack configuration can be checked |
| Multi-Category Procurement | Liver, renal, cardiac and lipid reagents can be sourced together |
| Bangalore-Based Operations | Convenient for Bengaluru and Karnataka laboratories |
| India-Wide Supply | Delivery to serviceable locations across India |
| Direct Customer Support | Product and order queries handled directly |
It is used for quantitative measurement of ALT / SGPT activity in professional clinical chemistry laboratories.
The pack size is 50 ml.
It uses a Modified IFCC kinetic method.
The assay uses 340 nm.
The assay is performed at 37°C.
The macro method uses 100 µl, while the semi-micro method uses 50 µl.
The factor is 1746.
The stated linearity is 300 U/L.
Mix 4 parts R1 with 1 part R2.
The manufacturer states 7 days at 15–25°C and 4 weeks at 2–8°C.
No. It is intended for professional in vitro diagnostic use only.
The laboratory should verify 340 nm capability, kinetic programming, reagent volumes and validated application before ordering.
It can be ordered through Microsidd India at microsidd.com, subject to stock availability.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
ALT / SGPT results should not be interpreted independently to diagnose or exclude liver disease. Clinical interpretation should consider SGOT / AST, bilirubin, alkaline phosphatase, symptoms, patient history and other relevant investigations.
The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory SOPs, calibration requirements, quality-control procedures or professional medical judgement.
Specimen requirements, reagent preparation, analyser settings, reference intervals, storage, stability and interpretation must always follow current manufacturer instructions and validated laboratory procedures.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
