The Swemed LDH Reagent Kit 25 ml is a professional clinical biochemistry reagent intended for the quantitative determination of Lactate Dehydrogenase (LDH) activity in suitable laboratory specimens.
Swemed identifies its LDH assay as an Optimized DGKC method using the pyruvate-to-lactate reaction, with a kinetic measurement at 340 nm. The reagent system is liquid stable, ready to use, and designed for routine professional clinical chemistry laboratories.
This 25 ml pack is suitable for pathology laboratories, diagnostic centres, hospitals and clinical chemistry facilities using compatible open-system semi-automatic and fully automatic biochemistry analysers, subject to application programming and laboratory validation.
| Product Detail | Information |
|---|---|
| Product Name | LDH Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 25 ml |
| Test Parameter | Lactate Dehydrogenase / LDH |
| Method | Optimized DGKC |
| Assay Type | Kinetic UV |
| Reaction | Pyruvate → Lactate |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Unit | U/L or IU/L |
| Reaction Direction | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 25 µl |
| Delay Time | 60 sec |
| Reaction Time | 180 sec |
| Delta Time | 60 sec |
| Factor | 8199 |
| Blanking | Water Blank |
| Manufacturer Insert Linearity | Up to 1000 U/L |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
Swemed's LDH insert specifies kinetic mode, 340 nm, 37°C, a factor of 8199, 1000 µl reagent, 25 µl sample, 60-second delay, 180-second reaction time and linearity to 1000 U/L.
Swemed's current catalogue additionally describes the LDH reagent as having high working-reagent stability up to 30 days, and lists a convenient 25 ml pack size.
Lactate Dehydrogenase (LDH) is an intracellular enzyme involved in the reversible conversion between lactate and pyruvate.
LDH is widely distributed in tissues including:
Because LDH occurs in many tissues, elevated LDH activity is not disease-specific and should always be interpreted with other laboratory findings and clinical information.
The Swemed LDH method is based on the conversion of pyruvate to lactate.
During the reaction, NADH is oxidised to NAD+. The decrease in NADH absorbance at 340 nm is measured kinetically.
The rate of decrease in absorbance is proportional to the LDH activity present in the sample.
Swemed's catalogue specifically describes its LDH assay as an Optimized DGKC method with pyruvate-to-lactate test reaction.
| Parameter | Swemed Specification |
|---|---|
| Test | LDH |
| Method | Optimized DGKC |
| Mode | Kinetic |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Unit | IU/L |
| Factor | 8199 |
| Reaction Slope | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 25 µl |
| Delay Time | 60 sec |
| Reaction Time | 180 sec |
| Delta Time | 60 sec |
| Blanking | Water Blank |
| Linearity | 1000 U/L |
Swemed's LDH insert provides the following macro application:
| Component | Volume |
|---|---|
| Reagent R1 | 800 µl |
| Reagent R2 | 200 µl |
| Sample | 25 µl |
Mix well and read the initial absorbance after the specified delay, then continue absorbance readings at one-minute intervals.
The average absorbance change per minute is used to calculate LDH activity.
For the Swemed application:
LDH activity = ΔA/min × Factor
Manufacturer factor:
8199
The exact analyser application should be confirmed using the current Swemed insert supplied with the reagent lot.
Swemed's LDH insert states that the assay is linear up to:
1000 U/L
For specimens above the validated range, the manufacturer instructs dilution 1 + 5 with physiological saline, repeating the test and multiplying the result by 6.
Swemed's newer product catalogue describes LDH as having high linearity up to 1200 IU/L. Because manufacturer documents differ, laboratories should follow the current lot-specific IFU supplied with the product as the definitive specification.
Swemed's LDH insert provides a serum reference range of:
230–460 U/L
The manufacturer also recommends that each laboratory establish or verify its own reference interval according to its patient population, analyser and analytical system.
The Swemed LDH Reagent Kit can be used on compatible programmable open-system chemistry analysers that support kinetic UV measurement at 340 nm.
Suitable categories include:
Typical application requirements include:
| Setting | LDH Application |
|---|---|
| Mode | Kinetic |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Sample | 25 µl |
| Reagent | 1000 µl |
| Delay | 60 sec |
| Reaction Time | 180 sec |
| Reaction Direction | Decreasing |
| Factor | 8199 |
The laboratory must validate the method on its own analyser before routine patient reporting.
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine enzyme testing |
| Diagnostic Centres | Clinical chemistry |
| Hospital Laboratories | LDH estimation |
| Cardiology Laboratories | Enzyme profile testing |
| Hepatology Laboratories | Clinical chemistry investigations |
| Oncology Laboratories | General biochemical monitoring |
| Clinical Chemistry Labs | Kinetic UV enzyme assays |
| Medical Institutions | Teaching and diagnostic use |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional reagent supply |
Microsidd India supplies LDH reagents, cardiac enzyme reagents and clinical biochemistry products from Bangalore / Bengaluru, Karnataka.
The Swemed LDH Reagent Kit 25 ml is suitable for pathology laboratories, diagnostic centres, hospital laboratories and clinical chemistry facilities using compatible open-system analysers.
It can be ordered through microsidd.com, subject to stock availability and serviceability.
Explore other Swemed enzyme and clinical chemistry reagents including CK-MB, CK-NAC, SGOT and SGPT.
Relevant buyer searches include:
| Entity | Details |
|---|---|
| Product | LDH Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 25 ml |
| Analyte | Lactate Dehydrogenase / LDH |
| Method | Optimized DGKC |
| Assay Type | Kinetic UV |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Factor | 8199 |
| Linearity | Manufacturer insert: up to 1000 U/L |
| Clinical Category | Enzyme / Clinical Chemistry |
| Product Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Analyser Compatibility | Compatible Open-System Fully Automatic & Semi-Automatic Analysers |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
The Swemed LDH Reagent Kit 25 ml is a professional clinical chemistry reagent used for quantitative measurement of Lactate Dehydrogenase activity.
Optimized DGKC Method.
A kinetic UV assay.
340 nm.
25 µl.
1000 µl.
Decreasing.
8199.
The manufacturer insert states 1000 U/L, while the newer catalogue describes high linearity up to 1200 IU/L. Follow the current lot-specific IFU for final application parameters.
Yes, on compatible open-system semi-automatic biochemistry analysers after method programming and validation.
Yes, on compatible open-system fully automatic chemistry analysers supporting the required kinetic UV application.
Laboratory reagents should be purchased only after confirming the correct analyte, methodology, pack size, wavelength, analyser compatibility and assay parameters.
Microsidd India focuses on professional diagnostic, laboratory and IVD products and provides Swemed reagents through dedicated Swemed and Biochemistry collections, making related reagent procurement easier for laboratories.
| Buying Factor | Microsidd.com | General Online Marketplaces | Offline Suppliers |
|---|---|---|---|
| Diagnostic Specialisation | Laboratory / IVD focused | Seller expertise varies | Dealer dependent |
| Method Verification | Optimized DGKC clearly identified | Listing dependent | Depends on dealer |
| Pack Verification | 25 ml clearly listed | May vary | Stock dependent |
| Open-System Guidance | Suitable for analyser-specific queries | Often limited | Depends on expertise |
| Related Swemed Reagents | Easy cross-navigation | Often scattered | Stock dependent |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for labs and institutions | Seller dependent | Usually suitable |
| Seller Identity | Microsidd India | Marketplace seller may vary | Usually identifiable |
It is used for quantitative determination of Lactate Dehydrogenase enzyme activity in professional clinical chemistry laboratories.
Optimized DGKC Method.
25 ml.
340 nm.
Kinetic.
25 µl.
1000 µl.
8199.
Decreasing.
The technical insert states 1000 U/L, while the current catalogue describes up to 1200 IU/L. Use the current lot-specific manufacturer IFU as the definitive value.
Yes, on compatible open-system semi-automatic biochemistry analysers after programming and validation.
Yes, on compatible open-system fully automatic analysers supporting the required kinetic UV method.
No. LDH is present in multiple tissues, so an abnormal value should be interpreted with other clinical and laboratory findings.
No. It is intended for professional in-vitro diagnostic laboratory use only.
The information on this page is provided for product identification, laboratory procurement and professional educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
LDH is a non-specific enzyme marker and should not be interpreted independently to diagnose or exclude cardiac, hepatic, haematological, muscular, oncological or other disorders.
For open-system fully automatic and semi-automatic analysers, instrument compatibility, reagent application settings, quality control and analytical performance must be independently verified and validated by the laboratory before routine patient reporting.
Because manufacturer documentation currently shows a difference between the technical insert and newer catalogue for maximum linearity, the current package insert supplied with the actual reagent lot should be treated as the definitive technical specification.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Reagent, Laboratory |
| Color pattern | White |
| Test method | Lab analysis |
| Test sample | Blood |
