The Swemed Phosphorus Reagent Kit 2 × 50 ml is a professional clinical biochemistry reagent intended for the quantitative determination of inorganic phosphorus in serum, heparin plasma and urine.
Swemed uses the UV-Phosphomolybdate method, in which inorganic phosphate reacts with ammonium molybdate in the presence of sulfuric acid to form non-reduced phosphomolybdate. The amount formed is directly proportional to the inorganic phosphate concentration and is measured by the increase in absorbance at 340 nm.
This product is suitable for professional pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and clinical chemistry facilities using compatible open-system fully automatic and semi-automatic biochemistry analysers after method programming and validation.
| Product Detail | Information |
|---|---|
| Product Name | Phosphorus Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Test Parameter | Inorganic Phosphorus / Phosphate |
| Method | UV-Phosphomolybdate |
| Mode | Endpoint |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Unit | mg/dL |
| Standard Concentration | 5 mg/dL |
| Reaction Direction | Increasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Incubation | 10 minutes at room temperature |
| Linearity | Up to 12 mg/dL |
| Specimens | Serum / Heparin Plasma / Urine |
| Reagent Preparation | Ready to Use |
| Storage | 18–30°C |
| Freezing | Do Not Freeze |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
Swemed's quick-reference section specifies endpoint mode, 340 nm, 37°C, a 5 mg/dL standard, 1000 µl reagent, 20 µl sample and linearity to 12 mg/dL.
Phosphorus is an essential mineral involved in:
Swemed notes that phosphorus is mainly associated with calcium in bone, with a smaller portion present as inorganic phosphorus or phosphate esters. The manufacturer literature also notes that abnormal phosphorus levels may be seen in disorders involving parathyroid function, renal failure, bone disease and vitamin D metabolism.
Phosphorus is commonly assessed together with:
Inorganic phosphate reacts with ammonium molybdate in the presence of sulfuric acid to form non-reduced phosphomolybdate.
The amount of phosphomolybdate formed is directly proportional to the phosphorus concentration in the specimen.
The assay is measured by the increase in absorbance at 340 nm.
Swemed states that:
The reagent and standard are ready to use.
No routine reagent reconstitution is required before performing the assay according to the manufacturer's procedure.
| Parameter | Swemed Specification |
|---|---|
| Test | Phosphorus |
| Method | UV-Phosphomolybdate |
| Mode | Endpoint |
| Wavelength | 340 nm |
| Unit | mg/dL |
| Temperature | 37°C |
| Standard Concentration | 5 mg/dL |
| Reaction Slope | Increasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Incubation | 10 minutes at RT |
| Blanking | Reagent Blank |
| Linearity | 12 mg/dL |
| Tube | Reagent | Standard / Sample |
|---|---|---|
| Blank | 1000 µl | — |
| Standard | 1000 µl | 20 µl Standard |
| Test | 1000 µl | 20 µl Sample |
Mix and incubate for:
10 minutes at room temperature
Then read the absorbance of the standard and sample against the reagent blank at:
340 nm.
Phosphorus (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration
Manufacturer standard concentration:
5 mg/dL.
Swemed states that the reaction is linear up to:
12 mg/dL
For specimens above this range, the manufacturer recommends dilution 1 + 4 with physiological saline, repeating the assay and multiplying the obtained result by 5.
The Swemed Phosphorus Reagent Kit can be used with compatible programmable open-system analysers where the required assay settings can be entered and validated.
| Open-System Setting | Phosphorus Application |
|---|---|
| Mode | Endpoint |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Reagent Volume | 1000 µl |
| Sample Volume | 20 µl |
| Standard | 5 mg/dL |
| Incubation | 10 minutes at RT |
| Reaction | Increasing |
| Blank | Reagent Blank |
| Unit | mg/dL |
Suitable analyser categories include:
The laboratory should verify analyser compatibility and validate the method before routine patient reporting.
Swemed lists the following specimen types:
The manufacturer recommends fasting serum specimens where appropriate because meals can lower inorganic phosphate levels.
Swemed states that:
Heparin is the only acceptable anticoagulant.
The following should be avoided because they may lower or interfere with inorganic phosphate measurement:
Serum and plasma should be separated from erythrocytes as soon as possible.
Swemed notes that delayed separation may allow inorganic phosphate and phosphate esters to leak from red cells into the surrounding sample medium.
Swemed recommends collecting urine in an acid-washed, detergent-free container and acidifying the specimen with hydrochloric acid to pH below 3.
The insert states that urine should be diluted before testing.
For routine assay preparation, Swemed specifies:
Dilute urine 1:10 (1 + 9) with distilled water and multiply the final result by 10.
| Storage Condition | Stability |
|---|---|
| 15–25°C | 1 day |
| 4–8°C | 4 days |
| −20°C | 1 year |
| Storage Condition | Stability |
|---|---|
| 15–25°C | 2 days |
| 2–8°C | 6 months |
Haemolysis should be avoided because red blood cells contain high levels of inorganic phosphate.
The manufacturer states that:
may interfere with the assay.
| Population / Specimen | Swemed Reference Range |
|---|---|
| Adults, fasting serum | 2.7–4.5 mg/dL |
| Children under 12 years | 4.5–5.5 mg/dL |
| Children under 1 year | 4.5–6.7 mg/dL |
| Urine, 24 hours | 0.3–1.0 g/24 h |
Swemed recommends that each laboratory verify appropriate reference intervals for its own population and analyser system.
Store the reagent:
Swemed notes that the reagent contains sodium azide at 0.95 g/L as preservative.
Professional users should:
Phosphorus fits naturally with the existing Swemed electrolyte and mineral reagent range.
| Parameter | Laboratory Role |
|---|---|
| Sodium | Extracellular fluid balance |
| Potassium | Intracellular / cardiac electrolyte balance |
| Chloride | Fluid and acid-base balance |
| Calcium | Bone and neuromuscular physiology |
| Magnesium | Mineral and metabolic function |
| Phosphorus | Bone, renal and cellular energy metabolism |
This reagent is therefore an important component of the Swemed Electrolyte & Mineral Reagent Combo.
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine phosphorus estimation |
| Diagnostic Centres | Renal and mineral profiles |
| Hospital Laboratories | Clinical chemistry |
| Nephrology Centres | Renal and phosphate investigations |
| Dialysis Centres | Mineral and renal monitoring |
| Endocrinology Laboratories | Calcium-phosphorus assessment |
| Clinical Chemistry Laboratories | Routine biochemical testing |
| Medical Institutions | Teaching and diagnostic use |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply |
Microsidd India supplies electrolyte, mineral-profile, renal-profile and clinical biochemistry reagents from Bangalore / Bengaluru, Karnataka.
The Swemed Phosphorus Reagent Kit 2 × 50 ml is suitable for pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and other professional clinical chemistry facilities.
It can be ordered through microsidd.com, subject to stock availability and delivery serviceability.
Also available as part of the Swemed Electrolyte & Mineral Reagent Combo containing Sodium, Potassium, Chloride, Calcium, Magnesium and Phosphorus.
Relevant buyer searches include:
| Entity | Details |
|---|---|
| Product | Phosphorus Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 2 × 50 ml |
| Analyte | Inorganic Phosphorus / Phosphate |
| Method | UV-Phosphomolybdate |
| Mode | Endpoint |
| Wavelength | 340 nm |
| Standard | 5 mg/dL |
| Linearity | 12 mg/dL |
| Clinical Category | Electrolyte / Mineral / Renal Profile |
| Product Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Analyser Compatibility | Open-System Fully Automatic & Semi-Automatic Analysers |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
The Swemed Phosphorus Reagent Kit 2 × 50 ml is a professional clinical chemistry reagent for quantitative measurement of inorganic phosphorus using the UV-Phosphomolybdate endpoint method.
340 nm.
20 µl.
1000 µl.
5 mg/dL.
10 minutes at room temperature.
Up to 12 mg/dL.
Serum, heparin plasma and urine.
EDTA, citrate and fluoride should be avoided because they can interfere with or lower inorganic phosphate measurement.
Yes. Swemed states that the reagent and standard are ready to use.
Yes, on compatible open-system semi-automatic biochemistry analysers after method programming and validation.
Yes, on compatible open-system fully automatic biochemistry analysers that permit third-party reagent programming and support 340 nm endpoint measurement.
Clinical chemistry reagents should be purchased only after confirming the correct parameter, methodology, pack size, analyser wavelength, specimen requirement, storage condition and seller source.
Microsidd India specialises in laboratory and diagnostic products and has long-standing experience in the IVD sector, helping professional buyers source related biochemistry reagents from one focused supplier.
| Buying Factor | Microsidd.com | General Online Marketplaces | Offline Suppliers |
|---|---|---|---|
| Diagnostic Specialisation | Laboratory / IVD focused | Seller expertise varies | Dealer dependent |
| Method Information | UV-Phosphomolybdate clearly specified | Listing dependent | Dealer dependent |
| Pack Verification | 2 × 50 ml clearly identified | May vary | Stock dependent |
| Open-System Guidance | Suitable for technical enquiries | Often limited | Depends on supplier |
| Related Reagents | Calcium, Magnesium, RFT, Electrolytes | Multiple unrelated listings | Stock dependent |
| GST Invoice | Available for eligible orders | Seller dependent | May vary |
| B2B Procurement | Suitable for laboratories | Seller dependent | Usually suitable |
| Seller Identity | Microsidd India | Marketplace seller may vary | Usually identifiable |
It is used for quantitative determination of inorganic phosphorus in serum, heparin plasma and urine.
UV-Phosphomolybdate Method.
2 × 50 ml.
340 nm.
Endpoint.
20 µl.
1000 µl.
5 mg/dL.
12 mg/dL.
Yes. Swemed provides a urine procedure and recommends appropriate acidification and dilution before analysis.
No. Swemed identifies heparin as the acceptable anticoagulant and advises avoiding EDTA, fluoride and citrate.
Store at 18–30°C, protect from light and contamination, and do not freeze.
Yes. It is the Phosphorus component of the Swemed Electrolyte & Mineral Reagent Combo together with Sodium, Potassium, Chloride, Calcium and Magnesium.
The information on this page is provided for product identification, professional laboratory procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
Phosphorus results should not be interpreted independently to diagnose or exclude renal, parathyroid, metabolic, bone or vitamin D-related disorders. Results should be correlated with calcium, magnesium, renal-function parameters, clinical history and other relevant investigations.
For open-system fully automatic and semi-automatic analysers, instrument compatibility, reagent programming, standardisation, quality control and analytical performance must be verified and validated by the laboratory before routine patient reporting.
The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Reagent, Laboratory |
| Color pattern | White |
| Product certifications & standards | CDSCO, ISO |
| Test method | Lab analysis |
| Test sample | Blood |
