Swemed Diagnostics

Magnesium Electrolyte Reagent Kit 2 × 50 ml – Xylidyl Blue / EGTA Method

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SKU: Magnesium 100ml
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Swemed Magnesium Electrolyte Reagent Kit 2 × 50 ml

The Swemed Magnesium Electrolyte Reagent Kit 2 × 50 ml is a professional clinical biochemistry reagent intended for the quantitative determination of magnesium in serum and plasma using the Xylidyl Blue method.

Swemed’s official literature states that magnesium ions react with Xylidyl Blue under alkaline conditions to form a purple-red chelate complex. The intensity of the colour produced is directly proportional to the magnesium concentration in the specimen.

Swemed’s current catalogue lists Magnesium 2 × 50 ml, product code MAG 717, with EGTA as the stated method classification and a 24-month shelf life.

This product is intended for professional pathology laboratories, diagnostic centres, hospitals and clinical chemistry laboratories, including compatible open-system fully automatic and semi-automatic biochemistry analysers after method programming and laboratory validation.

Product at a Glance

Product Detail Information
Product Name Magnesium Electrolyte Reagent Kit
Brand Swemed Diagnostics
Pack Size 2 × 50 ml
Product Code MAG 717
Test Parameter Magnesium
Method Xylidyl Blue / EGTA Method
Assay Mode Endpoint
Wavelength 505 nm
Temperature 37°C
Unit mg/dL
Standard Concentration 2.0 mg/dL
Reaction Direction Increasing
Reagent Volume 1000 µl
Sample Volume 10 µl
Incubation 10 minutes at room temperature
Measuring Range 1–5.0 mg/dL
Linearity Up to 5.0 mg/dL
Specimen Serum / Heparin Plasma
Reagent Preparation Ready to Use
Reagent Storage 18–30°C
Freezing Do Not Freeze
Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers
Intended Use Professional In Vitro Diagnostic Use
Seller Microsidd India
Website microsidd.com
Location Bangalore / Bengaluru, Karnataka, India

The assay parameters above are provided in Swemed’s official Magnesium insert.

Key Features

  • Swemed Diagnostics Magnesium Reagent Kit
  • Pack size: 2 × 50 ml
  • Product code: MAG 717
  • Xylidyl Blue / EGTA method
  • Ready-to-use reagent
  • Ready-to-use magnesium standard
  • Endpoint colorimetric assay
  • Measurement wavelength: 505 nm
  • Standard concentration: 2.0 mg/dL
  • Sample volume: 10 µl
  • Reagent volume: 1000 µl
  • Incubation: 10 minutes at room temperature
  • Measuring range: 1–5.0 mg/dL
  • Linearity up to 5.0 mg/dL
  • Suitable for serum and heparin plasma
  • Compatible with open-system semi-auto analysers
  • Compatible with open-system fully automatic analysers
  • For professional in vitro diagnostic use only

Why Magnesium Testing Is Important

Magnesium is a major intracellular cation and plays an important role in:

  • Enzyme activation
  • Protein synthesis
  • Amino-acid activation
  • Neuromuscular function
  • Electrolyte balance
  • Renal and metabolic physiology

Swemed notes that increased magnesium levels may occur in conditions such as dehydration, Addison’s disease and uraemia, while decreased levels may be seen in malabsorption, chronic renal disease, chronic alcoholism, hyperthyroidism, pancreatitis and during treatment of diabetic coma.

Magnesium is commonly evaluated together with:

  • Sodium
  • Potassium
  • Chloride
  • Calcium
  • Phosphorus
  • Urea
  • Creatinine

Test Principle

Magnesium ions react with Xylidyl Blue in an alkaline medium to form a coloured magnesium-Xylidyl Blue complex.

The intensity of the purple-red colour formed is proportional to the magnesium concentration in the specimen.

Reagent Composition

Component Manufacturer Specification
Tris Buffer pH 10 24.2 g/L
Xylidyl Blue 50.3 mg/L
Potassium Carbonate 10.3 g/L
EGTA 19.0 g/L
Sodium Azide 500 mg/L
Magnesium Standard 2.0 mg/dL

 

Reagent Preparation

Swemed states that:

The reagent and standard are ready to use.

This makes the product convenient for routine clinical chemistry workflows without reagent reconstitution.

Technical Specifications

Parameter Swemed Specification
Test Magnesium
Method Xylidyl Blue / EGTA
Mode Endpoint
Wavelength 505 nm
Unit mg/dL
Temperature 37°C
Standard Concentration 2.0 mg/dL
Reaction Slope Increasing
Reagent Volume 1000 µl
Sample Volume 10 µl
Incubation 10 minutes at room temperature
Blanking Reagent Blank
Measuring Range 1–5.0 mg/dL
Linearity 5.0 mg/dL

 

Assay Procedure Summary

Tube Reagent Standard / Sample
Blank 1000 µl
Standard 1000 µl 10 µl Standard
Test 1000 µl 10 µl Sample

Mix and incubate for:

10 minutes at room temperature

Then measure the absorbance of the sample and standard against the reagent blank at:

505 nm.

Calculation

Magnesium (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Concentration of Standard

Manufacturer standard:

2.0 mg/dL.

Compatible with Open-System Biochemistry Analysers

The Swemed Magnesium Reagent Kit can be used with compatible open-system analysers that allow the required endpoint application to be programmed.

Open-System Setting Magnesium Application
Mode Endpoint
Wavelength 505 nm
Temperature 37°C
Reagent Volume 1000 µl
Sample Volume 10 µl
Standard 2.0 mg/dL
Incubation 10 minutes at RT
Reaction Increasing
Blank Reagent Blank
Unit mg/dL

Suitable analyser categories include:

  • Open-system semi-automatic biochemistry analysers
  • Open-system fully automatic biochemistry analysers
  • Programmable chemistry systems supporting 505 nm endpoint measurement

The laboratory should verify analyser compatibility and validate the application before routine patient reporting.

Specimen Requirements

Swemed lists:

  • Serum
  • Heparin plasma

Fresh, clear, non-haemolysed serum is recommended.

This is particularly important because erythrocytes contain higher magnesium concentrations than serum, so haemolysis can produce falsely elevated magnesium results. Swemed advises that visibly haemolysed specimens should not be used.

Anticoagulants to Avoid

Swemed advises avoiding:

  • EDTA
  • Sodium fluoride
  • Oxalate

because these substances can interfere with the assay.

The manufacturer also notes that trace chelating agents such as EDTA, oxalate and citrate can prevent formation of the colour complex.

Specimen Stability

Specimen Stability
Serum / Plasma at 2–8°C 7 days

Contaminated specimens should be discarded.

Measuring Range and Linearity

The assay is designed to measure magnesium concentrations in the range:

1–5.0 mg/dL

The stated linearity is:

5.0 mg/dL.

For samples above the measuring range, Swemed recommends dilution 1 + 1 with 0.9% NaCl, repeating the test and multiplying the obtained result by 2.

Important Laboratory Precautions

Swemed specifically recommends:

  • Avoid visibly haemolysed specimens
  • Separate serum from the clot promptly
  • Avoid chelating agents such as EDTA, oxalate and citrate
  • Use clean glassware
  • Rinse glassware with 1% or 0.1 N HCl, followed by good-quality deionised water before testing

These precautions are especially important in magnesium testing because trace contamination or chelation can affect analytical performance.

Manufacturer Reference Range

Population Manufacturer Reference Range
Newborn 1.2–2.6 mg/dL
Children 1.5–2.3 mg/dL
Women 1.9–2.5 mg/dL
Men 1.8–2.6 mg/dL

Swemed recommends that each laboratory establish or verify its own reference interval for its patient population.

Storage and Stability

Store the reagent:

  • At 18–30°C
  • Protected from light
  • Protected from contamination
  • Do not freeze
  • Use only within the labelled expiry date

Swemed lists possible signs of deterioration as:

  • Reagent blank absorbance at 505 nm above 1.0
  • Visible particles
  • Turbidity

The current Swemed catalogue lists a 24-month shelf life for Magnesium when stored correctly.

Safety and Precautions

The reagent contains sodium azide preservative and should be handled using routine laboratory safety precautions.

Professional users should:

  • Wear suitable gloves
  • Use eye/face protection
  • Avoid skin or mucous-membrane contact
  • Never pipette by mouth
  • Follow current Good Laboratory Practice
  • Dispose of reagent according to institutional procedures

Magnesium in an Electrolyte / Renal Profile

Parameter Laboratory Relevance
Magnesium Intracellular mineral and enzyme-related function
Sodium Extracellular fluid balance
Potassium Cardiac and intracellular electrolyte balance
Chloride Fluid and acid-base balance
Calcium Mineral and neuromuscular function
Phosphorus Bone and renal metabolism
Urea Renal and protein metabolism
Creatinine Renal filtration assessment

Who Can Buy This Product?

Buyer Type Typical Application
Pathology Laboratories Routine magnesium testing
Diagnostic Centres Electrolyte and metabolic testing
Hospital Laboratories Clinical chemistry investigations
Nephrology Centres Renal and electrolyte monitoring
Dialysis Centres Mineral and electrolyte testing
Endocrinology Centres Metabolic and electrolyte investigation
Clinical Chemistry Laboratories Routine magnesium estimation
Medical Institutions Teaching and diagnostic laboratory use
Healthcare Organisations Institutional procurement
Laboratory Distributors Professional supply where permitted

Useful Tips Before Ordering

  • Confirm that Magnesium – Xylidyl Blue / EGTA Method is required.
  • Verify the pack size: 2 × 50 ml.
  • Confirm analyser support for 505 nm endpoint measurement.
  • Verify that your analyser is an open system.
  • Confirm sample volume: 10 µl.
  • Confirm reagent volume: 1000 µl.
  • Verify the standard concentration: 2.0 mg/dL.
  • Avoid haemolysed samples.
  • Avoid EDTA, oxalate, citrate and sodium fluoride contamination.
  • Store reagent at 18–30°C.
  • Do not freeze.
  • Perform calibration and internal quality control before routine use.

Buy Swemed Magnesium Reagent in Bangalore

Microsidd India supplies electrolyte reagents, renal-profile reagents and clinical biochemistry products from Bangalore / Bengaluru, Karnataka.

The Swemed Magnesium Electrolyte Reagent Kit 2 × 50 ml is suitable for pathology laboratories, diagnostic centres, hospitals, nephrology facilities, dialysis centres and professional clinical chemistry laboratories using compatible open-system analysers.

The product can be ordered through microsidd.com, subject to stock availability and serviceability.

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Product Summary

What is Swemed Magnesium Reagent Kit?

The Swemed Magnesium Electrolyte Reagent Kit 2 × 50 ml is a ready-to-use professional clinical chemistry reagent for quantitative magnesium determination using the Xylidyl Blue / EGTA endpoint method.

What method is used?

Swemed’s product insert identifies the assay as the Xylidyl Blue Method, while the current catalogue classifies the product method as EGTA.

What wavelength is required?

505 nm.

What is the sample volume?

10 µl.

What is the reagent volume?

1000 µl.

What is the magnesium standard concentration?

2.0 mg/dL.

What is the measuring range?

1–5.0 mg/dL.

What is the linearity?

5.0 mg/dL.

Which specimens can be used?

Serum and heparin plasma.

Why should haemolysed samples be avoided?

Red blood cells contain more magnesium than serum, so haemolysis can cause falsely elevated results.

Is the reagent ready to use?

Yes. Swemed states that the reagent and standard are ready to use.

Can it be used on semi-automatic analysers?

Yes, on compatible open-system semi-automatic biochemistry analysers after method programming and validation.

Can it be used on fully automatic analysers?

Yes, on compatible open-system fully automatic biochemistry analysers that allow third-party reagent programming and support the required endpoint parameters.

Information

Entity Details
Product Magnesium Electrolyte Reagent Kit
Brand Swemed Diagnostics
Pack Size 2 × 50 ml
Product Code MAG 717
Analyte Magnesium
Method Xylidyl Blue / EGTA
Mode Endpoint
Wavelength 505 nm
Unit mg/dL
Specimen Serum / Heparin Plasma
Clinical Category Electrolytes / Renal / Metabolic Profile
Product Category Laboratory Reagents
Subcategory Biochemistry Reagents
Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Analysers
Seller Microsidd India
City Bangalore / Bengaluru
State Karnataka
Country India
Website microsidd.com

Why Buy Swemed Magnesium Reagent from Microsidd.com?

Electrolyte and mineral reagents should be purchased only after verifying the correct analyte, methodology, wavelength, sample type, pack size, storage requirements and analyser compatibility.

Microsidd India specialises in laboratory and diagnostic products and has long-standing experience in the IVD sector, helping professional buyers verify technical requirements before ordering.

Microsidd.com vs Online Marketplaces vs Offline Suppliers

Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers
Seller Specialisation Diagnostic & laboratory focused Seller expertise varies Dealer dependent
Method Verification Xylidyl Blue / EGTA can be confirmed Listing details may be limited Depends on dealer
Pack Verification 2 × 50 ml can be checked Pack listing may vary Stock dependent
Open-System Guidance Better suited to analyser-specific enquiries Often limited Dealer dependent
Product Support Technical parameters can be checked before ordering General support Depends on supplier
GST Invoice Available for eligible purchases Seller dependent May vary
B2B Procurement Suitable for labs and institutions Seller dependent Usually local
Product Range Specialist electrolyte and biochemistry catalogue Broad but not specialised Often limited
Customer Support Direct Microsidd support Marketplace + seller layer Local support
India-Wide Supply Subject to serviceability Broad Usually regional

Frequently Asked Questions

What is Swemed Magnesium Reagent used for?

It is used for quantitative determination of magnesium in serum and plasma using the Xylidyl Blue method.

What is the pack size?

2 × 50 ml.

What method does it use?

Xylidyl Blue / EGTA Method.

What wavelength is required?

505 nm.

What is the assay mode?

Endpoint.

What is the sample volume?

10 µl.

What is the reagent volume?

1000 µl.

What is the standard concentration?

2.0 mg/dL.

What is the measuring range?

1–5.0 mg/dL.

What is the linearity?

5.0 mg/dL.

Is the reagent ready to use?

Yes. Both the reagent and standard are ready to use.

Can haemolysed samples be used?

No. Swemed advises against visibly haemolysed specimens because erythrocytes contain high magnesium concentrations.

Which anticoagulants should be avoided?

EDTA, sodium fluoride and oxalate should be avoided. Trace citrate can also interfere because chelating agents prevent formation of the colour complex.

How should the reagent be stored?

At 18–30°C, protected from light and contamination. Do not freeze.

Can it be used on a semi-auto analyser?

Yes, on compatible open-system semi-automatic biochemistry analysers after programming and validation.

Can it be used on a fully automatic analyser?

Yes, on compatible open-system fully automatic analysers supporting the required endpoint application.

Is this for home testing?

No. It is intended for professional in vitro diagnostic use only.

Medical and Laboratory Disclaimer

The information on this product page is provided for general product identification, procurement and laboratory educational purposes only.

This product is intended for professional in vitro diagnostic use by trained laboratory personnel.

Magnesium results should not be interpreted independently to diagnose or exclude renal, endocrine, gastrointestinal, metabolic or electrolyte disorders. Results should be correlated with clinical history, calcium, potassium, renal-function tests and other relevant investigations.

Because haemolysis, chelating agents and glassware contamination can significantly affect magnesium measurement, correct specimen handling and laboratory technique are especially important.

For open-system fully automatic and semi-automatic analysers, instrument compatibility, application settings, calibration, quality control and analytical performance must be verified and validated by the laboratory before routine patient reporting.

The current Swemed package insert supplied with the actual product lot should always be treated as the definitive technical specification.

No Return / No Exchange Policy

This product is non-returnable and non-exchangeable.

Please verify the brand, model, compatibility, pack size and product specifications before placing your order.

For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.

Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.

An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.

Product specifications table
Specification name Specification Value
Chemical grade Laboratory, Reagent
Test method Lab analysis
Test sample Blood

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