The Swemed Urea UV Reagent Kit is a professional clinical biochemistry reagent intended for the photometric quantitative determination of urea in serum, plasma and urine using the GLDH enzymatic method.
The assay is performed as a fixed-time UV method at 340 nm, where urea is first hydrolysed by urease to ammonia, and the ammonia then reacts with α-ketoglutarate in a reaction catalysed by glutamate dehydrogenase (GLDH). During this reaction, NADH is oxidised to NAD+, producing a decrease in absorbance proportional to the amount of urea present in the sample.
For your Microsidd Shopify listing, the product is available in 25 ml, 50 ml and 100 ml pack-size variants.
The reagent is suitable for compatible open-system fully automatic and semi-automatic biochemistry analysers that permit programming of fixed-time applications and the required wavelength, volumes and timing parameters.
| Variant | Pack Size |
|---|---|
| Swemed Urea UV | 25 ml |
| Swemed Urea UV | 50 ml |
| Swemed Urea UV | 100 ml |
| Product Detail | Information |
|---|---|
| Product Name | Urea UV Reagent Kit |
| Brand | Swemed Diagnostics |
| Available Pack Sizes | 25 ml, 50 ml, 100 ml |
| Test Parameter | Urea |
| Method | GLDH Method |
| Assay Type | UV Enzymatic |
| Mode | Fixed Time |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Standard Concentration | 50 mg/dL |
| Reaction Direction | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 10 µl |
| Delay Time | 30 seconds |
| Reaction Time | 60 seconds |
| Linearity | Up to 300 mg/dL |
| Specimen | Serum / Plasma / Fresh Urine |
| Working Reagent Ratio | 4 parts R1 + 1 part R2 |
| Storage | 2–8°C |
| Analyser Compatibility | Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The assay settings above are taken from the current Swemed Urea UV technical insert.
The GLDH method provides a direct enzymatic UV approach to urea measurement and is widely used in modern clinical chemistry systems.
The Swemed Urea UV assay uses a defined 30-second delay and 60-second reaction interval, supporting straightforward programming on compatible open-system analysers.
With 25 ml, 50 ml and 100 ml variants, laboratories can choose the pack size that best matches their daily workload and reagent turnover.
The reagent can be programmed on compatible open-system fully automatic and semi-automatic analysers, subject to validation by the laboratory.
Urea UV is commonly used together with:
The Swemed Urea UV Reagent is suitable for compatible open-system analysers that allow third-party reagent programming.
| Analyser Parameter | Swemed Urea UV Setting |
|---|---|
| Mode | Fixed Time |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Standard Concentration | 50 mg/dL |
| Reaction Direction | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 10 µl |
| Delay Time | 30 sec |
| Reaction Time | 60 sec |
| Blank | Water Blank |
| Linearity | 300 mg/dL |
| Unit | mg/dL |
These are the quick-reference parameters provided in the current Swemed insert.
Compatibility and application performance should be validated by the laboratory before routine patient testing.
The Swemed Urea UV Reagent Kit is intended for the quantitative photometric determination of urea in serum, plasma or urine.
Swemed lists suitable specimen types including:
The product is intended exclusively for professional in vitro diagnostic use.
Urea is enzymatically hydrolysed by urease:
Urea + H₂O → Ammonium ions + CO₂
The resulting ammonium ions react with α-ketoglutarate and NADH in the presence of GLDH.
During this reaction, NADH is oxidised to NAD+, leading to a decrease in absorbance at 340 nm.
The magnitude of the absorbance change is proportional to the concentration of urea in the sample.
| Component | Manufacturer Specification |
|---|---|
| R1 – Tris Buffer | 9 g/L |
| R1 – Urease | 30 kU/L |
| R1 – GLDH | 4 kU/L |
| R1 – α-Ketoglutarate | 3.4 g/L |
| R2 – NADH | 1.1 g/L |
| Standard | Urea UV 50 mg/dL |
Prepare the working reagent by mixing:
4 parts R1 + 1 part R2
After mixing, Swemed instructs laboratories to leave the working reagent for at least 30 minutes at 15–25°C before use.
| Storage Condition | Stability |
|---|---|
| 15–25°C | 5 days |
| 2–8°C | 4 weeks |
Protect the prepared working reagent from light.
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | Urea UV |
| Method | GLDH |
| Mode | Fixed Time |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Unit | mg/dL |
| Standard | 50 mg/dL |
| Reaction Slope | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 10 µl |
| Delay Time | 30 sec |
| Reaction Time | 60 sec |
| Blanking | Water Blank |
| Linearity | 300 mg/dL |
| Component | Macro Method | Semi-Micro Method |
|---|---|---|
| R1 + R2 | 800 µl + 200 µl | 400 µl + 100 µl |
| Standard / Sample | 10 µl | 5 µl |
Mix and incubate for approximately 30 seconds at 37°C, then record the first absorbance reading.
After exactly another 60 seconds, record the second absorbance reading.
Urea (mg/dL) = ΔA Sample ÷ ΔA Standard × Concentration of Standard
Manufacturer standard:
50 mg/dL
Swemed recommends:
Important precautions include:
Fresh urine can be tested after dilution.
Swemed instructs laboratories to:
Dilute urine 1 + 49 with distilled water
Then:
Multiply the final result by 50.
| Storage Condition | Stability |
|---|---|
| 20–25°C | 2 days |
| 4–8°C | 7 days |
| −20°C | 1 month |
| Storage Condition | Stability |
|---|---|
| 20–25°C | 2 days |
| 2–8°C | 5 days |
| −20°C | 1 month |
The Swemed Urea UV assay is stated to measure approximately:
2.0–300 mg/dL
with linearity up to:
300 mg/dL.
If the result exceeds the linearity limit, the manufacturer recommends dilution 1:2 with 0.9% NaCl solution and multiplication of the obtained result by 3.
Swemed reports no significant interference under the evaluated conditions from:
| Interferent | Tested Level |
|---|---|
| Bilirubin | Up to 40 mg/dL |
| Haemoglobin | Up to 500 mg/dL |
| Ascorbate | Up to 30 mg/dL |
| Triglycerides | Up to 2000 mg/dL |
| Specimen | Manufacturer Reference |
|---|---|
| Serum / Plasma | 14–40 mg/dL |
| Urine | Up to 20 g/L |
Each laboratory should verify its own population-specific reference interval.
Store the reagents:
Swemed states that reagent deterioration may be indicated by visible particles, turbidity or inappropriate blank absorbance at 340 nm.
| Feature | Urea UV | Urea Berthelot |
|---|---|---|
| Method | GLDH | Berthelot |
| Measurement | UV Fixed Time | Colorimetric Endpoint |
| Wavelength | 340 nm | 578 nm |
| Reaction Direction | Decreasing | Increasing |
| Sample Volume | 10 µl | 10 µl |
| Standard | 50 mg/dL | 50 mg/dL |
| Linearity | 300 mg/dL | 300 mg/dL |
| Best For | UV-capable chemistry analysers | Colorimetric endpoint analysers |
Customers should select the method that matches their analyser setup and validated laboratory workflow. Swemed lists Urea UV and Urea Berthelot as separate products.
Urea is a major end product of protein metabolism. It is formed in the liver from ammonia produced during amino-acid breakdown and is transported to the kidneys for excretion.
Urea testing is commonly used in:
Urea results should be interpreted together with creatinine, electrolytes, clinical history and other relevant findings.
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine urea UV testing |
| Diagnostic Centres | Renal profile testing |
| Hospital Laboratories | Clinical chemistry testing |
| Nephrology Centres | Renal-function investigations |
| Dialysis Centres | Renal biochemical assessment |
| Clinical Chemistry Laboratories | Routine UV chemistry |
| Medical Institutions | Teaching and diagnostic use |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies renal-profile reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed Urea UV Reagent Kit is available on microsidd.com in 25 ml, 50 ml and 100 ml variants and is suitable for compatible open-system fully automatic and semi-automatic analysers.
The product is available for professional laboratory procurement, subject to stock availability and delivery serviceability.
Relevant search phrases include:
The Swemed Urea UV Reagent is a professional clinical biochemistry reagent for quantitative measurement of urea using the GLDH enzymatic fixed-time method at 340 nm.
GLDH Method.
340 nm.
10 µl.
1000 µl.
50 mg/dL.
Up to 300 mg/dL.
Mix 4 parts R1 with 1 part R2.
Yes, when used on compatible open-system semi-automatic analysers after programming and validation.
Yes, on compatible open-system fully automatic analysers supporting 340 nm fixed-time chemistry and third-party reagent programming.
| Entity | Details |
|---|---|
| Product | Urea UV Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Sizes | 25 ml / 50 ml / 100 ml |
| Analyte | Urea |
| Method | GLDH |
| Mode | Fixed Time |
| Wavelength | 340 nm |
| Specimen | Serum / Plasma / Urine |
| Clinical Category | Renal Profile |
| Product Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Analyser Compatibility | Open-System Fully Automatic & Semi-Automatic Analysers |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
Urea reagents need to be selected carefully because Urea UV and Urea Berthelot are different methodologies and analyser compatibility matters.
Microsidd India focuses on professional laboratory, diagnostic and healthcare products and has long-standing experience in the IVD sector. This helps laboratory buyers verify the correct parameter, method, pack size and open-system application before ordering.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic & laboratory focused | Seller expertise varies | Dealer dependent |
| Method Verification | Urea UV vs Berthelot can be checked | Similar listings may confuse buyers | Depends on supplier |
| Pack Variants | 25 ml / 50 ml / 100 ml listed clearly | Listing may vary | Stock dependent |
| Open-System Guidance | Suitable for analyser-specific queries | Often limited | Dealer dependent |
| Seller Identity | Direct Microsidd India purchase | Marketplace seller may vary | Usually identifiable |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for professional labs | Seller dependent | Usually local |
| Product Range | Specialist biochemistry catalogue | Broad but not specialised | Often limited |
| Direct Support | Microsidd support | Marketplace + third-party seller | Local support |
| India-Wide Supply | Subject to serviceability | Broad | Usually regional |
It is used for quantitative determination of urea in serum, plasma and urine using the GLDH method.
This Microsidd listing includes:
25 ml, 50 ml and 100 ml.
GLDH Method.
340 nm.
Fixed Time.
50 mg/dL.
10 µl.
60 seconds, after a 30-second delay.
The assay is linear up to 300 mg/dL.
Yes. Fresh urine should be diluted 1 + 49 with distilled water, and the final result multiplied by 50.
Yes, on compatible open-system semi-automatic biochemistry analysers after programming and validation.
Yes, on compatible open-system fully automatic analysers that support 340 nm fixed-time measurement and third-party reagent programming.
No. Urea UV uses GLDH and UV measurement at 340 nm, while Urea Berthelot is a colorimetric endpoint method at 578 nm.
The information on this page is provided for product identification, procurement and professional laboratory education only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
Urea results should not be interpreted independently to diagnose or exclude kidney disease or any other medical condition. Results should be correlated with creatinine, electrolytes, clinical history and other relevant investigations.
For open-system fully automatic and semi-automatic analysers, analyser compatibility, application settings, calibration and performance must be verified and validated by the laboratory before routine patient testing.
The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
