Swemed Diagnostics

Uric Acid Reagent Kit – 25 ml, 50 ml & 100 ml | Uricase / PAP Method

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SKU: Uric Acid25
Pack Size: 25ml
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Swemed Uric Acid Reagent Kit

The Swemed Uric Acid Reagent Kit is a professional clinical biochemistry reagent intended for the quantitative determination of uric acid in serum, plasma and fresh urine using the Uricase / PAP enzymatic method.

Swemed's technical literature describes an enzymatic reaction in which uricase converts uric acid to allantoin and hydrogen peroxide. The hydrogen peroxide subsequently reacts with a phenolic compound and 4-aminoantipyrine in the presence of peroxidase to form a red quinoneimine dye. The intensity of the resulting colour is directly proportional to the amount of uric acid present in the specimen.

For the Microsidd Shopify listing, this product is offered in convenient 25 ml, 50 ml and 100 ml pack-size options.

Swemed's current catalogue lists Uric Acid 25 ml and 2 × 50 ml under product code UA 724, while the technical insert also documents 2 × 25 ml and 2 × 50 ml configurations. This supports laboratory procurement across 25 ml, 50 ml and 100 ml total reagent quantities depending on the supplied configuration.

Also available as part of the Swemed RFT Reagent Combo Kit


Available Pack Sizes

Variant Reagent Configuration
25 ml Compact 25 ml presentation
50 ml 2 × 25 ml configuration
100 ml 2 × 50 ml configuration

Swemed's technical insert identifies UA50 for 2 × 25 ml and UA100 for 2 × 50 ml, while its current catalogue also lists a 25 ml presentation under product code UA 724.

Product at a Glance

Product Detail Information
Product Name Uric Acid Reagent Kit
Brand Swemed Diagnostics
Available Pack Sizes 25 ml / 50 ml / 100 ml
Test Parameter Uric Acid
Method Uricase / PAP
Assay Type Enzymatic Colorimetric
Mode Endpoint
Wavelength 505 nm
Temperature 37°C
Standard Concentration 5 mg/dL
Reaction Direction Increasing
Reagent Volume 1000 µl
Sample Volume 50 µl
Incubation 10 minutes at 37°C
Measuring Range 1–25 mg/dL
Linearity Up to 25 mg/dL
Specimens Serum / Heparin Plasma / EDTA Plasma / Fresh Urine
Reagent Preparation Ready to Use
Storage 2–8°C
Freezing Do Not Freeze
Intended Use Professional In Vitro Diagnostic Use
Seller Microsidd India
Website microsidd.com
Location Bangalore / Bengaluru, Karnataka, India

The analytical parameters above are specified in the Swemed Uric Acid technical insert.

Key Features

  • Swemed Diagnostics Uric Acid Reagent Kit
  • Available in 25 ml, 50 ml and 100 ml
  • Established Uricase / PAP enzymatic method
  • Ready-to-use reagent
  • Ready-to-use uric acid standard
  • Endpoint colorimetric assay
  • Recommended wavelength: 505 nm
  • Standard concentration: 5 mg/dL
  • Reagent volume: 1000 µl
  • Sample volume: 50 µl
  • Incubation: 10 minutes at 37°C
  • Measuring range: 1–25 mg/dL
  • Linearity up to 25 mg/dL
  • Suitable for serum and compatible plasma
  • Suitable for fresh urine
  • Suitable for compatible open-system semi-auto analysers
  • Suitable for compatible open-system fully automatic analysers
  • For professional in vitro diagnostic use only

Key Benefits

Enzymatic Uricase Method

The assay uses uricase and peroxidase-based chemistry, providing an established enzymatic method for quantitative uric acid measurement.

Ready-to-Use Reagent

Swemed states that both the reagent and standard are ready to use, simplifying routine laboratory workflow.

Multiple Pack Choices

The availability of 25 ml, 50 ml and 100 ml total reagent options allows laboratories to choose according to testing volume, reagent turnover and workload.

Useful for Renal and Metabolic Profiles

Uric acid is frequently included alongside:

  • Urea
  • Creatinine
  • Sodium
  • Potassium
  • Chloride
  • Calcium
  • Other renal and metabolic parameters

Open-System Compatibility

The assay parameters can be programmed on compatible open-system semi-automatic and fully automatic biochemistry analysers, subject to validation by the laboratory.

Intended Use

The Swemed Uric Acid Reagent Kit is intended for quantitative measurement of uric acid in professional clinical chemistry laboratories.

The manufacturer technical insert lists:

  • Serum
  • Heparin plasma
  • EDTA plasma
  • Fresh urine

Swemed's online biochemistry catalogue also identifies Uric Acid as a Uricase/PAP method assay for serum and urine.

This product is for professional in vitro diagnostic use only.

Test Principle

The assay proceeds through two main enzymatic reactions.

First:

Uric Acid + H₂O —Uricase→ Allantoin + H₂O₂

The hydrogen peroxide formed then reacts with 4-aminoantipyrine and a phenolic compound in the presence of peroxidase to produce a red quinoneimine dye.

The intensity of the colour formed is proportional to the amount of uric acid present in the sample.

Reagent Composition

According to Swemed's product literature:

Component Manufacturer Specification
Uricase 200 U/L
Peroxidase 4 kU/L
BSA 1 g/L
Triton X-100 4 ml/L
Uric Acid Standard 5 mg/dL

 

Reagent Preparation

The reagent and standard are ready to use.

No routine reagent reconstitution is required before following the manufacturer assay procedure.

Technical Specifications

Parameter Swemed Specification
Test Uric Acid
Method Uricase / PAP
Mode Endpoint
Wavelength 505 nm
Unit mg/dL
Temperature 37°C
Standard 5 mg/dL
Reaction Slope Increasing
Reagent Volume 1000 µl
Sample Volume 50 µl
Incubation 10 minutes at 37°C
Blanking Reagent Blank
Measuring Range 1–25 mg/dL
Linearity 25 mg/dL

 

Assay Procedure Summary

Tube Reagent Standard / Sample
Blank 1000 µl
Standard 1000 µl 50 µl Standard
Test 1000 µl 50 µl Sample

Mix and incubate for:

10 minutes at 37°C

Then read the absorbance against the reagent blank at:

505 nm.

Calculation

Uric Acid (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration

Standard concentration:

5 mg/dL.

Compatible with Open-System Biochemistry Analysers

The Swemed Uric Acid Reagent Kit can be used with compatible programmable open-system analysers where the required assay parameters can be entered and validated.

Open-System Setting Uric Acid Application
Method Uricase / PAP
Mode Endpoint
Wavelength 505 nm
Temperature 37°C
Reagent Volume 1000 µl
Sample Volume 50 µl
Standard 5 mg/dL
Incubation 10 minutes
Reaction Increasing
Blank Reagent Blank
Unit mg/dL

Suitable system categories include:

  • Open-system semi-automatic biochemistry analysers
  • Open-system fully automatic biochemistry analysers
  • Programmable clinical chemistry systems supporting 505 nm endpoint measurement

The laboratory should verify analyser compatibility and validate the reagent application before routine patient reporting.

Specimen Requirements

Swemed lists the following suitable specimens:

  • Serum
  • Heparin plasma
  • EDTA plasma
  • Fresh urine

The manufacturer recommends using fresh, clear, non-haemolysed serum from fasting patients where appropriate.

Bacterial contamination should be avoided because it may affect uric acid concentration during storage.

Specimen Stability

According to the Swemed technical insert:

Storage Condition Stability
20–25°C 2 days
2–8°C 3–5 days
−20°C 6 months

Contaminated specimens should be discarded.

Measuring Range and Linearity

The assay is designed for uric acid concentrations between:

1–25 mg/dL

The stated linearity is:

25 mg/dL.

If a result exceeds the measuring range, Swemed instructs laboratories to dilute the sample 1:1 with 0.9% NaCl solution, repeat the assay and multiply the obtained result by 2.

Interfering Substances

Swemed identifies several important analytical considerations.

Ascorbic Acid

Elevated ascorbic-acid concentrations may produce falsely depressed uric acid values.

Lipemia

Lipemic samples may cause falsely elevated results.

Haemoglobin

Haemoglobin up to approximately 100 mg/dL was reported to have negligible influence within the manufacturer's stated tolerance, while higher concentrations may produce falsely elevated uric acid values.

Bilirubin

Bilirubin up to 30 mg/dL was reported to have negligible interference under the evaluated assay conditions.

Manufacturer Reference Range

Group Swemed Reference Range
Men 3.4–7.0 mg/dL
Women 2.5–6.0 mg/dL

Swemed recommends that each laboratory establish or verify its own reference interval appropriate to its patient population.

Storage and Stability

Store the reagent:

  • At 2–8°C
  • Protected from light
  • Protected from contamination
  • Do not freeze
  • Use within the labelled expiry period
  • Bring reagents and specimens to room temperature as required before testing

The current Swemed catalogue lists 24-month stability for its Uric Acid reagent when stored correctly.

Signs of Reagent Deterioration

The manufacturer advises against using the reagent if:

  • It becomes visibly turbid
  • Obvious microbial growth is present
  • The reagent blank absorbance reaches 0.250 or higher at 505 nm

Safety and Precautions

Swemed states that the reagent contains sodium azide at 0.95 g/L as preservative.

Professional laboratory users should:

  • Wear suitable gloves
  • Use appropriate eye / face protection
  • Avoid skin and mucous-membrane contact
  • Never pipette by mouth
  • Follow Good Laboratory Practice
  • Handle reagent waste according to institutional procedures

Why Uric Acid Testing Is Important

Uric acid is the final product of purine metabolism and is eliminated mainly through the kidneys.

Swemed notes that increased uric acid concentrations may occur in conditions including gout and impaired renal function, while reduced levels may occur in selected metabolic or hepatic disorders.

Uric acid testing is commonly used as part of:

  • Renal-function assessment
  • Hyperuricaemia investigation
  • Gout assessment
  • Metabolic profiles
  • Routine health screening
  • Monitoring of selected therapies
  • Kidney-function profiles
  • Clinical chemistry investigations

Laboratory results should always be interpreted in the appropriate clinical context.

Uric Acid in an RFT / Renal Profile

Parameter Laboratory Relevance
Uric Acid Purine metabolism and renal excretion
Urea Protein metabolism and renal excretion
Creatinine Renal filtration-related biochemical marker
Sodium Fluid and electrolyte balance
Potassium Intracellular / renal electrolyte balance
Chloride Fluid and acid-base balance
Calcium Mineral metabolism
Magnesium Mineral and metabolic balance

This product is therefore particularly suitable for linking internally to your upcoming Swemed RFT Reagent Combo Kit.

Who Can Buy This Product?

Buyer Type Typical Application
Pathology Laboratories Routine uric acid estimation
Diagnostic Centres Renal and metabolic profiles
Hospital Laboratories Clinical chemistry
Nephrology Centres Renal-profile investigations
Rheumatology Centres Uric acid-related investigations
Gout Clinics Hyperuricaemia assessment
Diabetes Centres Metabolic and renal profiles
Clinical Chemistry Laboratories Routine enzymatic testing
Medical Institutions Teaching and diagnostic laboratory use
Laboratory Distributors Professional procurement

Useful Tips Before Ordering

  • Confirm that Uric Acid – Uricase / PAP Method is required.
  • Select the appropriate pack size: 25 ml, 50 ml or 100 ml.
  • Confirm that your analyser supports 505 nm endpoint measurement.
  • Verify that the analyser is an open system before programming third-party reagents.
  • Confirm sample volume: 50 µl.
  • Confirm reagent volume: 1000 µl.
  • Verify standard concentration: 5 mg/dL.
  • Avoid contaminated specimens.
  • Evaluate haemolysis and lipemia before testing.
  • Store reagent at 2–8°C.
  • Do not freeze.
  • Perform suitable calibration / standardisation and internal QC before routine use.

Buy Swemed Uric Acid Reagent in Bangalore

Microsidd India supplies renal-function reagents, biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka.

The Swemed Uric Acid Reagent Kit is available in 25 ml, 50 ml and 100 ml total reagent options, making it suitable for laboratories with different testing volumes.

It can be ordered through microsidd.com, subject to stock availability and delivery serviceability.

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Product Summary

What is Swemed Uric Acid Reagent Kit?

The Swemed Uric Acid Reagent Kit is a professional clinical biochemistry reagent for quantitative uric acid determination using the Uricase / PAP enzymatic endpoint method.

Which pack sizes are available?

Microsidd lists:

  • 25 ml
  • 50 ml
  • 100 ml

The manufacturer literature documents a 25 ml presentation as well as 2 × 25 ml and 2 × 50 ml configurations across its current catalogue and technical insert.

What method is used?

Uricase / PAP Method.

What wavelength is required?

505 nm.

What is the sample volume?

50 µl.

What is the reagent volume?

1000 µl.

What is the standard concentration?

5 mg/dL.

What is the incubation time?

10 minutes at 37°C.

What is the measuring range?

1–25 mg/dL.

What is the linearity?

25 mg/dL.

Is the reagent ready to use?

Yes. Swemed states that both reagent and standard are ready to use.

Which specimens can be tested?

Serum, heparin plasma, EDTA plasma and fresh urine are listed in the manufacturer insert.

Is it compatible with semi-automatic analysers?

Yes, on compatible open-system semi-automatic biochemistry analysers after method programming and laboratory validation.

Is it compatible with fully automatic analysers?

Yes, on compatible open-system fully automatic biochemistry analysers that permit third-party reagent programming and support the required endpoint application.

Entity Information

Entity Details
Product Uric Acid Reagent Kit
Brand Swemed Diagnostics
Pack Sizes 25 ml / 50 ml / 100 ml
Manufacturer Product Code UA 724
Analyte Uric Acid
Method Uricase / PAP
Mode Endpoint
Wavelength 505 nm
Standard 5 mg/dL
Measuring Range 1–25 mg/dL
Clinical Category Renal / Metabolic Profile
Product Category Laboratory Reagents
Subcategory Biochemistry Reagents
Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Analysers
Seller Microsidd India
City Bangalore / Bengaluru
State Karnataka
Country India
Website microsidd.com

Why Buy Swemed Uric Acid Reagent from Microsidd.com?

Clinical chemistry reagents should be purchased only after verifying the correct parameter, methodology, pack configuration, wavelength, sample requirements, storage conditions and analyser compatibility.

Microsidd India specialises in laboratory and diagnostic products and has long-standing experience in the IVD sector, allowing professional laboratory buyers to purchase from an identifiable diagnostic-product supplier rather than relying only on generic marketplace listings.

Microsidd.com vs Online Marketplaces vs Offline Suppliers

Buying Factor Microsidd.com General Online Marketplaces Offline Suppliers
Diagnostic Specialisation Laboratory / IVD focused Seller expertise varies Dealer dependent
Pack Options 25 ml / 50 ml / 100 ml clearly organised Listings may vary Stock dependent
Method Verification Uricase / PAP clearly identified Seller dependent Dealer dependent
Analyser Guidance Better suited to open-system queries Often limited Depends on technical knowledge
Seller Identity Microsidd India Marketplace seller may vary Usually identifiable
GST Invoice Available for eligible orders Seller dependent May vary
RFT Integration Links naturally with Urea & Creatinine Usually separate listings Dealer dependent
Product Information Technical specifications available Listing dependent Often verbal
B2B Procurement Suitable for laboratories and institutions Seller dependent Usually suitable
India-Wide Supply Subject to serviceability Broad Often regional

Benefits of Buying from Microsidd.com

Microsidd Advantage Benefit to Laboratory Buyers
Diagnostic Product Specialisation Better understanding of laboratory procurement
Multiple Pack Sizes Choose according to laboratory workload
Technical Product Information Method and assay settings available before ordering
Direct Seller Accountability Purchase directly from Microsidd India
RFT Product Range Urea, Creatinine and Uric Acid can be sourced together
Open-System Focus Suitable for fully and semi-auto analyser users
GST Invoice Useful for institutional purchasing
Bangalore-Based Operations Convenient for Bengaluru / Karnataka laboratories
India-Wide Supply Delivery to serviceable locations
Direct Product Support Easier handling of product and order queries

Frequently Asked Questions

What is Swemed Uric Acid Reagent used for?

It is used for quantitative measurement of uric acid in serum, plasma and fresh urine using the enzymatic Uricase method.

What pack sizes are available?

Microsidd lists 25 ml, 50 ml and 100 ml variants.

What method is used?

Uricase / PAP.

What wavelength is required?

505 nm.

What is the assay mode?

Endpoint.

What is the reagent volume?

1000 µl.

What is the sample volume?

50 µl.

What is the standard concentration?

5 mg/dL.

How long is the incubation?

10 minutes at 37°C.

What is the linearity?

25 mg/dL.

Is the reagent ready to use?

Yes. The reagent and standard are ready to use.

Can fresh urine be used?

Yes. Fresh urine is listed as a suitable specimen in the Swemed product insert.

How should the reagent be stored?

At 2–8°C, protected from light and contamination. Do not freeze.

Can the reagent be used on a semi-auto analyser?

Yes, on compatible open-system semi-automatic biochemistry analysers after programming and validation.

Can it be used on a fully automatic analyser?

Yes, on compatible open-system fully automatic biochemistry analysers supporting the assay requirements and third-party reagent programming.

Is this product useful for an RFT panel?

Yes. Uric Acid is commonly used alongside Urea and Creatinine in renal and metabolic testing.

Is this suitable for home testing?

No. This reagent is intended for professional in vitro diagnostic laboratory use only.

Medical and Laboratory Disclaimer

The information on this page is provided for product identification, professional laboratory procurement and educational purposes only.

This product is intended for professional in vitro diagnostic use by trained laboratory personnel.

Uric acid results should not be interpreted independently to diagnose or exclude gout, kidney disease, metabolic disorders or other medical conditions. Results should be evaluated together with clinical history, Urea, Creatinine, renal-function parameters and other relevant investigations.

Specimen quality, lipemia, haemolysis, bilirubin and ascorbic acid may influence assay results under certain conditions.

For open-system fully automatic and semi-automatic analysers, instrument compatibility, assay programming, standardisation, calibration or standard use, quality control and analytical performance must be verified and validated by the laboratory before routine patient reporting.

The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification.

No Return / No Exchange Policy

This product is non-returnable and non-exchangeable.

Please verify the brand, model, compatibility, pack size and product specifications before placing your order.

For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.

Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.

An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.

Product specifications table
Specification name Specification Value
Chemical grade Reagent, Laboratory
Color pattern White
Product certifications & standards CDSCO, ISO
Test method Lab analysis
Test sample Blood

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