The Swemed HDL Cholesterol Reagent Kit is a professional direct enzymatic liquid biochemistry reagent intended for the quantitative determination of HDL Cholesterol (HDL-C) in serum and plasma.
The assay uses a two-reagent direct enzymatic method designed to selectively measure HDL cholesterol without requiring a separate precipitation step. It is intended for use in pathology laboratories, diagnostic centres, hospitals and clinical biochemistry laboratories performing routine lipid profile testing.
HDL cholesterol is an important component of lipid-profile assessment. High-density lipoproteins participate in cholesterol transport, and HDL-C measurement is commonly used together with total cholesterol, triglycerides and LDL cholesterol in cardiovascular-risk evaluation.
| Product Detail | Information |
|---|---|
| Product Name | HDL Cholesterol Reagent Kit |
| Brand | Swemed Diagnostics |
| Reagent Type | Direct Enzymatic Liquid |
| Test Parameter | HDL Cholesterol / HDL-C |
| Assay Format | Two-Reagent System |
| Test Type | Direct Enzymatic / Photometric |
| Measurement Mode | Endpoint |
| Reaction Direction | Increasing |
| Specimen | Serum, EDTA Plasma, Heparin Plasma |
| Wavelength | 630 nm |
| Reaction Temperature | 37°C |
| Manual Incubation | 10 minutes at room temperature |
| Sample Volume | 10 µl |
| Reagent 1 Volume | 800 µl |
| Reagent 2 Volume | 200 µl |
| Linearity | Up to 180 mg/dL |
| Reagent Preparation | Ready to Use |
| Calibrator | Lyophilized Human Serum |
| Storage | 2–8°C |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The Swemed Direct HDL Cholesterol system consists of:
| Component | Description |
|---|---|
| Reagent 1 | Enzyme Reagent |
| Reagent 2 | Developer Reagent |
| Calibrator | Lyophilized Human Serum |
The reagents are supplied ready to use. The calibrator is reconstituted with the exact volume of deionized water stated on its label, mixed thoroughly and allowed to stand at room temperature for approximately 30 minutes before use.
The assay uses selective detergent chemistry and enzymatic reactions to enable direct determination of HDL cholesterol without a manual lipoprotein precipitation procedure.
Reagent 1 and Reagent 2 are supplied ready for laboratory use, helping simplify routine HDL testing workflows.
The assay uses only 10 µl of sample, making it suitable for routine clinical chemistry workflows where efficient specimen utilisation is important.
HDL-C is commonly measured together with:
This makes the reagent highly relevant for laboratories performing routine lipid-profile investigations.
Suitable for pathology laboratories, hospital laboratories, diagnostic centres and other professional clinical chemistry facilities.
The Swemed HDL Cholesterol Direct Enzymatic Liquid Reagent is intended for quantitative determination of HDL cholesterol in serum and plasma.
The product is intended for use by trained laboratory professionals and for in vitro diagnostic use only.
The method uses a two-reagent system based on selective detergent and enzymatic reactions.
In the first reagent, non-HDL unesterified cholesterol undergoes an enzyme reaction. The hydrogen peroxide produced during this stage is consumed through a peroxidase reaction, yielding a colourless product.
The second reagent contains a detergent that selectively solubilizes HDL, together with cholesterol esterase and a chromogenic coupler. This produces a coloured reaction proportional to the HDL cholesterol concentration present in the specimen.
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | HDL Cholesterol |
| Method | Direct Enzymatic |
| Assay Format | Two Reagents |
| Mode | Endpoint |
| Wavelength | 630 nm |
| Reaction Temperature | 37°C |
| Reaction Direction | Increasing |
| Sample Volume | 10 µl |
| Reagent 1 Volume | 800 µl |
| Reagent 2 Volume | 200 µl |
| Delay | 5 seconds |
| Manual Incubation | 10 minutes at room temperature |
| Linearity | 180 mg/dL |
| Addition | Blank | Calibrator | Test |
|---|---|---|---|
| HDL Reagent 1 | 800 µl | 800 µl | 800 µl |
| HDL Reagent 2 | 200 µl | 200 µl | 200 µl |
| Calibrator | — | 10 µl | — |
| Sample | — | — | 10 µl |
Mix and incubate for 10 minutes at room temperature, then measure the absorbance of the sample and calibrator against the reagent blank at 630 nm.
Suitable specimens include:
Fresh serum is recommended. Serum should be removed from the blood clot as soon as possible.
Citrate-containing anticoagulants should not be used.
The manufacturer states that samples are stable for approximately:
| Storage Condition | Stability |
|---|---|
| 2–8°C | 7 days |
The Swemed Direct HDL Cholesterol method is linear up to 180 mg/dL.
Samples with concentrations above this limit may be diluted 1:1, with the resulting value multiplied by 2 according to the manufacturer procedure.
Store the HDL Cholesterol reagents at:
2–8°C
When stored correctly, the reagents remain stable until the expiration date stated on the reagent bottle and kit-box label.
HDL cholesterol is one of the major lipoprotein-related parameters used in clinical lipid assessment.
HDL particles participate in the transport of cholesterol in the bloodstream. HDL-C measurement is commonly used as part of cardiovascular risk assessment and lipid-profile interpretation. The Swemed insert describes HDL cholesterol measurement as a useful predictor in coronary heart disease evaluation.
HDL-C testing may therefore form part of:
Individual results should always be interpreted by qualified healthcare professionals in conjunction with other lipid parameters and the patient's clinical information.
| Parameter | Laboratory Relevance |
|---|---|
| Total Cholesterol | Measures overall circulating cholesterol |
| HDL Cholesterol | Measures cholesterol associated with high-density lipoproteins |
| LDL Cholesterol | Commonly used in atherogenic-risk evaluation |
| Triglycerides | Measures circulating triglyceride concentration |
| VLDL | Often estimated as part of lipid-profile interpretation |
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine HDL-C and lipid-profile testing |
| Diagnostic Centres | Cardiovascular and metabolic investigations |
| Hospital Laboratories | Clinical chemistry and lipid testing |
| Clinics with Laboratory Facilities | Routine biochemical screening |
| Preventive Health Centres | Lipid-profile investigations |
| Medical Institutions | Laboratory and teaching applications |
| Healthcare Organisations | Professional diagnostic procurement |
| Laboratory Distributors | Professional supply where permitted |
| Clinical Chemistry Laboratories | Routine HDL cholesterol testing |
Microsidd India supplies biochemistry reagents, lipid-profile reagents and clinical laboratory products from Bangalore / Bengaluru, Karnataka.
The Swemed HDL Cholesterol Direct Enzymatic reagent is suitable for procurement by:
Customers outside Bangalore can also order laboratory products through microsidd.com for delivery to serviceable locations across India.
This product is relevant for buyers searching for:
The Swemed HDL Cholesterol Direct Enzymatic Liquid Reagent is a professional two-reagent clinical biochemistry assay for quantitative determination of HDL cholesterol in serum and plasma.
It uses a direct enzymatic two-reagent method involving selective detergent chemistry to measure HDL cholesterol without a separate precipitation step.
Fresh serum, EDTA plasma and heparinized plasma are acceptable. Citrate-containing anticoagulants should not be used.
The manufacturer specifies 630 nm.
The assay uses 10 µl of sample.
The stated linearity is 180 mg/dL.
Yes. Swemed states that the reagents are supplied ready to use.
It can be ordered from Microsidd India through microsidd.com, subject to stock availability and delivery serviceability.
| Entity | Details |
|---|---|
| Product | HDL Cholesterol Reagent |
| Brand | Swemed Diagnostics |
| Method | Direct Enzymatic |
| Assay Format | Two-Reagent Liquid |
| Analyte | HDL Cholesterol / HDL-C |
| Specimen | Serum / EDTA Plasma / Heparin Plasma |
| Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Clinical Category | Lipid Profile |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Healthcare / B2B |
Laboratory reagents require more careful procurement than ordinary consumer products. Buyers should verify the correct analyte, method, reagent configuration, analyser compatibility, storage requirements, product source, expiry and seller accountability before placing an order.
Microsidd India has been associated with IVD and diagnostic-product supply since 2000 and focuses on diagnostic, laboratory and healthcare products. This specialist background makes microsidd.com suitable for laboratories that prefer purchasing from an identifiable diagnostic supplier rather than an unrelated marketplace seller.
Microsidd also maintains established and authorised manufacturer relationships across multiple diagnostic-product categories, providing professional buyers with a dependable procurement channel for recurring laboratory requirements.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic, laboratory and healthcare focused | Expertise varies by seller | Depends on dealer |
| Laboratory Product Knowledge | Product-focused support | Often general marketplace support | Dealer dependent |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and actual seller may differ | Usually identifiable locally |
| Manufacturer Relationships | Established diagnostic manufacturer relationships across multiple categories | Depends on seller | Varies |
| Product Verification | Method, analyte and specifications can be checked before ordering | Similar listings may cause confusion | Depends on available stock |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for laboratories and institutions | Seller dependent | Commonly local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but not specialised | Usually limited by local inventory |
| Customer Support | Direct Microsidd support | Marketplace plus seller layer | Local support |
| Accountability | One identifiable specialist supplier | Sellers may vary | Depends on supplier |
| Online Convenience | Direct ordering through microsidd.com | Convenient | May require store visit or phone ordering |
| Geographic Reach | India-wide subject to serviceability | Broad | Typically local or regional |
| Microsidd Advantage | Benefit to Laboratory Buyers |
|---|---|
| Specialist Diagnostic Supplier | Better alignment with professional laboratory requirements |
| IVD Experience Since 2000 | Long-standing diagnostic-product experience |
| Manufacturer Relationships | Established and authorised relationships across diagnostic categories |
| Direct Seller Accountability | Purchase directly from Microsidd India |
| GST Invoice | Suitable for professional and institutional procurement |
| Product Verification Support | Buyers can confirm specifications before ordering |
| Multi-Category Procurement | Reagents, diagnostic kits and consumables available together |
| Bangalore-Based Operations | Convenient for Bengaluru and Karnataka laboratories |
| India-Wide Supply | Useful for laboratories outside Bangalore |
| Direct Customer Support | Product and order queries handled by Microsidd |
It is intended for quantitative determination of HDL cholesterol in serum and plasma using a direct enzymatic method.
Yes. The Swemed product uses a direct enzymatic two-reagent method.
Fresh serum, EDTA plasma and heparinized plasma are acceptable.
No. The manufacturer states that anticoagulants containing citrate should not be used.
The assay is measured at 630 nm.
The manufacturer specifies 10 µl.
The specified automated parameters are 800 µl Reagent 1 and 200 µl Reagent 2.
The method is linear up to 180 mg/dL.
Yes. The reagents are supplied ready to use.
Yes. The lyophilized calibrator should be reconstituted using the exact volume of deionized water specified on its label and allowed to stand at room temperature for approximately 30 minutes.
Store at 2–8°C according to the manufacturer instructions.
The manufacturer states sample stability of 7 days at 2–8°C.
No. It is intended for professional in vitro diagnostic use only.
The laboratory should verify analyser compatibility, wavelength, reagent programming and validated application before ordering.
The product can be ordered from Microsidd India through microsidd.com, subject to availability.
Yes. Microsidd supplies diagnostic and laboratory products to serviceable locations across India.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
The information provided by Microsidd India does not replace the official Swemed Diagnostics product insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.
Specimen requirements, methodology, reagent preparation, analyser settings, calibration, reference intervals, storage, stability, quality-control requirements and interpretation should always follow the current manufacturer instructions.
Laboratory results must be interpreted by qualified healthcare professionals together with the patient's clinical information and other relevant investigations.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
