The Swemed Cholesterol Reagent Kit is a professional clinical biochemistry reagent intended for photometric quantitative determination of cholesterol in serum and plasma using the CHOD/POD enzymatic method.
The assay is based on enzymatic conversion of cholesterol esters to free cholesterol, oxidation of cholesterol to produce hydrogen peroxide, and subsequent formation of a coloured quinoneimine complex through the action of peroxidase. The intensity of the colour formed is directly proportional to the amount of cholesterol present in the specimen.
This reagent is intended for professional in vitro diagnostic use only and is suitable for routine use in pathology laboratories, diagnostic centres, hospital laboratories and clinical biochemistry facilities.
| Product Detail | Information |
|---|---|
| Product Name | Cholesterol Reagent Kit |
| Brand | Swemed Diagnostics |
| Method | CHOD/POD Method |
| Product Type | Biochemistry Reagent |
| Test Parameter | Total Cholesterol |
| Test Type | Photometric / Enzymatic |
| Measurement Mode | Endpoint |
| Specimen | Serum, Heparin Plasma, EDTA Plasma |
| Wavelength | 505 nm |
| Standard Concentration | 200 mg/dl |
| Reagent Volume | 1000 µl |
| Sample Volume | 10 µl |
| Incubation | 10 minutes at 37°C |
| Linearity | Up to 700 mg/dl |
| Reagent Preparation | Ready to Use |
| Storage | 2–8°C |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The technical assay specifications above are based on the Swemed Diagnostics product literature.
The Swemed literature lists the following kit configurations:
| Reference | Cholesterol Reagent | Standard |
|---|---|---|
| CHOL100 | 2 × 50 ml | 1 |
| CHOL200 | 2 × 100 ml | 1 |
The reagent uses a widely established enzymatic approach involving cholesterol esterase, cholesterol oxidase and peroxidase, making it suitable for routine total cholesterol testing.
The Swemed product insert states that both the reagent and standard are supplied ready to use, helping reduce preparation steps in routine laboratory workflows.
The assay has a stated linearity of 700 mg/dl, allowing routine testing across a broad cholesterol concentration range.
Total cholesterol is commonly evaluated as part of a broader lipid profile alongside triglycerides, HDL cholesterol and LDL cholesterol.
Suitable for trained professionals working in clinical chemistry laboratories with appropriate calibration, quality control and analyser procedures.
The Swemed Cholesterol Reagent Kit is intended for photometric quantitative determination of cholesterol in serum and plasma using the CHOD/POD method.
The manufacturer lists the following suitable specimens:
Cholesterol esterase hydrolyses cholesterol esters to free cholesterol.
The free cholesterol is then oxidised by cholesterol oxidase, producing hydrogen peroxide.
Hydrogen peroxide reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to form a red-coloured quinoneimine dye complex.
The intensity of the colour produced is directly proportional to the cholesterol concentration in the specimen.
| Component | Manufacturer Specification |
|---|---|
| Buffer | PIPES Buffer, pH 7.0 |
| Cholesterol Esterase | 300 U/L |
| Cholesterol Oxidase | 500 U/L |
| Peroxidase | 2 kU/L |
| Cholesterol Standard | 200 mg/dl |
| Test Parameter | Specification |
|---|---|
| Parameter | Cholesterol |
| Method | CHOD/POD |
| Mode | Endpoint |
| Wavelength | 505 nm |
| Unit | mg/dl |
| Temperature | 37°C |
| Standard Concentration | 200 mg/dl |
| Reaction Slope | Increasing |
| Reagent Volume | 1000 µl |
| Sample / Standard Volume | 10 µl |
| Incubation Time | 10 minutes at 37°C |
| Blanking | Reagent Blank |
| Linearity | 700 mg/dl |
| Tube | Reagent | Sample / Standard |
|---|---|---|
| Blank | 1000 µl | — |
| Standard | 1000 µl | 10 µl Standard |
| Test | 1000 µl | 10 µl Sample |
Mix and incubate for 10 minutes at 37°C, then read the absorbance against the reagent blank at 505 nm.
Laboratories should always follow their validated analyser settings and the current manufacturer package insert.
The manufacturer recommends fresh, clear, non-haemolysed serum from fasting patients for cholesterol estimation.
Suitable specimens include:
The insert notes that fluoride and oxalate anticoagulants may cause falsely elevated values.
According to the Swemed literature, cholesterol in serum or plasma is stable for approximately:
| Storage Condition | Stability |
|---|---|
| 20–25°C | 2 days |
| 2–8°C | 7 days |
| −20°C | 3 months |
The assay is stated to be linear up to 700 mg/dl.
For samples exceeding this concentration, the manufacturer describes dilution 1 + 4 with 0.9% NaCl solution, followed by multiplication of the result by 5.
The manufacturer reports no significant interference under the evaluated conditions from:
The insert also advises against using haemolysed or lipemic serum.
| Total Cholesterol | Interpretation |
|---|---|
| < 200 mg/dl | Desirable |
| 200–240 mg/dl | Borderline High Risk |
| > 240 mg/dl | High Risk |
The manufacturer also advises that each laboratory should verify whether the stated reference ranges are appropriate for its own patient population.
The kit components should be stored:
The reagent and standard should be brought to room temperature before use.
Signs of possible reagent deterioration include:
Cholesterol is an important lipid associated with cell membrane formation, steroid synthesis and lipid transport through lipoproteins.
Total cholesterol measurement forms an important part of laboratory lipid assessment and may contribute to the evaluation of:
Clinical interpretation should always be performed together with other lipid parameters and relevant patient information.
| Parameter | Laboratory Relevance |
|---|---|
| Total Cholesterol | Overall circulating cholesterol |
| HDL Cholesterol | Commonly used in cardiovascular risk assessment |
| LDL Cholesterol | Commonly associated with atherogenic risk evaluation |
| Triglycerides | Assessment of circulating triglyceride levels |
| VLDL | Often estimated as part of lipid interpretation |
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine cholesterol testing |
| Diagnostic Centres | Lipid profile investigations |
| Hospital Laboratories | Clinical chemistry testing |
| Clinics with Laboratory Facilities | Routine biochemical screening |
| Medical Institutions | Laboratory procurement and teaching use where applicable |
| Healthcare Organisations | Professional diagnostic procurement |
| Laboratory Distributors | Professional supply where permitted |
| Clinical Biochemistry Laboratories | Routine lipid testing |
Microsidd India supplies biochemistry and clinical chemistry reagents from Bangalore / Bengaluru, Karnataka to pathology laboratories, diagnostic centres, hospitals and professional healthcare buyers.
The Swemed Cholesterol Reagent Kit is available for professional procurement through microsidd.com, with delivery across Bangalore, Karnataka and other serviceable locations in India.
The Swemed Cholesterol Reagent Kit is a ready-to-use professional clinical biochemistry reagent for quantitative photometric determination of total cholesterol using the CHOD/POD enzymatic method.
The manufacturer lists serum, heparin plasma and EDTA plasma.
The assay uses 505 nm.
The Cholesterol Standard concentration is 200 mg/dl.
The stated linearity is 700 mg/dl.
Yes. The manufacturer states that the reagent and standard are ready to use.
The product can be ordered through Microsidd India at microsidd.com, subject to stock availability and serviceability.
| Entity | Details |
|---|---|
| Product | Cholesterol Reagent Kit |
| Brand | Swemed Diagnostics |
| Method | CHOD/POD |
| Analyte | Total Cholesterol |
| Category | Laboratory Reagents |
| Subcategory | Biochemistry Reagents |
| Clinical Area | Lipid Profile / Clinical Chemistry |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | B2B / Laboratory / Healthcare |
Laboratory reagents should be purchased with attention to the exact brand, methodology, pack configuration, analyser compatibility, storage requirements, expiry and seller reliability.
Microsidd India has been involved in the IVD and diagnostic-product sector since 2000 and specialises in diagnostic, laboratory and healthcare products rather than operating as a general-purpose marketplace.
Microsidd also maintains established manufacturer and authorised brand relationships across multiple diagnostic categories, giving professional buyers a specialist procurement option with direct seller accountability.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Focus | Specialist diagnostic and laboratory supplier | Seller expertise varies | Depends on dealer |
| Product Understanding | Better suited to laboratory-product queries | Often general marketplace support | Varies considerably |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and seller may differ | Usually identifiable |
| Manufacturer Relationships | Established diagnostic manufacturer relationships across multiple categories | Seller dependent | Dealer dependent |
| Product Verification | Buyers can verify brand, method and pack before ordering | Similar listings may cause confusion | Possible if physical stock is available |
| GST Invoice | Available for eligible purchases | Depends on seller | Availability varies |
| B2B Procurement | Suitable for laboratories, hospitals and diagnostic centres | Seller dependent | Usually local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but less specialised | Limited to local stock |
| Customer Support | Direct Microsidd support | Marketplace plus seller layer | Local support |
| Accountability | One identifiable specialist supplier | Seller can vary | Depends on dealer |
| Online Convenience | Direct ordering through microsidd.com | Convenient | Often phone or store based |
| Geographic Reach | India-wide delivery subject to serviceability | Broad | Usually regional |
Microsidd India supplies products across multiple diagnostic categories, including:
Microsidd’s diagnostic-product experience helps laboratories source multiple requirements through one specialist seller rather than depending on unrelated marketplace vendors.
It uses the CHOD/POD enzymatic method for quantitative photometric cholesterol determination.
The manufacturer lists serum, heparin plasma and EDTA plasma.
Yes. The reagent and standard are supplied ready to use.
The assay is read at 505 nm.
The Cholesterol Standard concentration is 200 mg/dl.
The assay procedure specifies 10 minutes at 37°C.
The reagent is stated to be linear up to 700 mg/dl.
Store at 2–8°C, tightly closed, protected from light and contamination, and do not freeze.
The manufacturer states that fluoride and oxalate anticoagulants may cause falsely elevated cholesterol values.
No. This product is intended for professional in vitro diagnostic use only.
Laboratories should verify analyser compatibility, wavelength, method and validated application before ordering.
The product can be purchased through Microsidd India at microsidd.com, subject to availability.
Yes. Microsidd supplies serviceable locations across India subject to stock and courier coverage.
Microsidd India specialises in diagnostic and laboratory products and offers direct seller accountability, GST invoicing for eligible purchases, professional product support and access to a growing laboratory-product catalogue.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
The information provided by Microsidd India does not replace the official Swemed Diagnostics product insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.
Specimen requirements, methodology, reagent preparation, analyser settings, calibration, reference ranges, storage conditions, stability, quality-control requirements and interpretation should always follow the current manufacturer instructions.
Laboratory results must be interpreted by qualified healthcare professionals together with the patient’s clinical history and other relevant investigations.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Laboratory, Reagent |
| Color pattern | White |
