The Swemed LDH Reagent Kit 25 ml is a professional clinical biochemistry reagent intended for quantitative determination of Lactate Dehydrogenase (LDH) activity in serum using a kinetic UV method.
Swemed’s current catalogue describes LDH under its cardiac reagent range as an Optimized DGKC method based on the pyruvate-to-lactate reaction, with liquid-stable ready-to-use reagents, high working-reagent stability and a convenient 25 ml pack size. The current Swemed LDH product literature labels the assay as a Modified IFCC Method. Both describe the same pyruvate-to-lactate kinetic LDH principle.
The test measures the rate at which NADH is oxidized to NAD+ during the LDH-catalysed conversion of pyruvate to lactate. The resulting decrease in absorbance is proportional to the LDH activity present in the specimen.
This product is intended for professional in vitro diagnostic use only in pathology laboratories, hospital laboratories, diagnostic centres and clinical chemistry facilities.
| Product Detail | Information |
|---|---|
| Product Name | LDH Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 25 ml |
| Reference | LDH25 |
| Product Type | Clinical Biochemistry Reagent |
| Test Parameter | Lactate Dehydrogenase / LDH |
| Method | Modified IFCC / Optimized DGKC Kinetic Method |
| Reaction | Pyruvate → Lactate |
| Measurement Type | UV Kinetic |
| Reaction Direction | Decreasing |
| Specimen | Serum, Heparin Plasma |
| Wavelength | 340 nm |
| Temperature | 37°C |
| Reagent Volume | 1000 µl |
| Sample Volume | 25 µl |
| Delay Time | 60 seconds |
| Reaction Time | 180 seconds |
| Reading Interval | 60 seconds |
| Calculation Factor | 8199 |
| Manufacturer Insert Linearity | Up to 1000 U/L |
| Storage | 2–8°C |
| Working Reagent | R1 + R2 |
| Intended Use | Professional In Vitro Diagnostic Use |
| Seller | Microsidd India |
| Website | microsidd.com |
| Location | Bangalore / Bengaluru, Karnataka, India |
The assay parameters above are from the official Swemed LDH product literature.
The Swemed LDH assay uses a kinetic UV method in which the decrease in NADH absorbance at 340 nm is measured over time, providing a direct rate-based estimate of LDH enzyme activity.
The kit uses R1 and R2, which can be combined in a 4:1 ratio to form the working reagent. The manufacturer also permits direct use of 0.8 ml R1 + 0.2 ml R2 during the assay.
Swemed specifically lists a 25 ml LDH pack for laboratories with routine or moderate LDH workloads. Its latest catalogue highlights this pack as a convenient size.
LDH measurement is used in a variety of laboratory investigations involving tissue injury and enzyme activity assessment.
The reagent is designed for trained laboratory personnel following validated analyser settings, internal quality control and current manufacturer instructions.
The Swemed LDH Reagent Kit 25 ml is intended for quantitative determination of Lactate Dehydrogenase activity in serum and compatible heparin plasma.
The product insert specifically identifies:
It also states that EDTA and sodium citrate should not be used because they may inhibit the assay.
LDH catalyses the reduction of pyruvate to lactate while NADH is oxidized to NAD+:
Pyruvate + NADH + H+ → Lactate + NAD+
The rate of decrease in absorbance caused by NADH oxidation is directly proportional to the amount of LDH activity present in the sample.
Swemed instructs laboratories to prepare the working reagent by mixing:
4 parts Reagent R1 + 1 part Reagent R2
The manufacturer states that the prepared working reagent remains stable for 1 week at 2–8°C.
An alternative direct assay setup is:
The combined reagent should have an absorbance above 1.0 against distilled water at 340 nm. If the absorbance is below this level, the reagent should be discarded.
| Component | Composition |
|---|---|
| Reagent 1 | Tris buffer pH 7.0 – 6 g/L |
| Reagent 1 | EDTA – 1.5 g/L |
| Reagent 1 | Sodium Pyruvate – 0.2 g/L |
| Reagent 1 | L-Alanine – 44.5 g/L |
| Reagent 2 | KH₂PO₄ – 0.9 g/L |
| Reagent 2 | K₂HPO₄ – 0.8 g/L |
The reagents also contain sodium azide as a preservative according to the manufacturer insert.
| Test Parameter | Manufacturer Specification |
|---|---|
| Parameter | LDH |
| Mode | Kinetic |
| Wavelength | 340 nm |
| Unit | IU/L |
| Temperature | 37°C |
| Reaction Slope | Decreasing |
| Reagent Volume | 1000 µl |
| Sample Volume | 25 µl |
| Reaction Time | 180 seconds |
| Delay Time | 60 seconds |
| Delta / Reading Time | 60 seconds |
| Blanking | Water Blank |
| Factor | 8199 |
| Linearity | 1000 U/L |
These quick-reference parameters are provided in the official Swemed LDH insert.
| Component | Macro Method | Semi-Micro Method |
|---|---|---|
| Reagent R1 + R2 | 800 + 200 µl | 400 + 100 µl |
| Sample | 25 µl | 12.5 µl |
Mix well and measure the initial absorbance after 1 minute, then repeat readings at approximately 1-minute intervals for 3 minutes. Calculate the mean absorbance change per minute.
LDH (U/L) = ΔA/min × 8199
Swemed recommends:
Important manufacturer precautions include:
The Swemed literature states:
| Specimen | Stability |
|---|---|
| Serum | Approximately 1–3 days at 2–8°C |
The manufacturer also notes that freezing can inactivate liver LDH isoenzymes, so specimen handling should follow the laboratory’s validated procedure.
The official LDH insert states that the method is linear up to 1000 U/L.
For samples exceeding this level, Swemed recommends dilution 1 + 5 with physiological saline, repeating the assay and multiplying the result by 6.
Swemed’s newer catalogue describes an updated LDH offering with high linearity up to 1200 IU/L and working-reagent stability up to 30 days. Because assay configuration may vary by production version, the current kit label and current package insert supplied with the 25 ml pack should be treated as the controlling specification.
| Specimen | Manufacturer Reference |
|---|---|
| Serum | 230–460 U/L |
Swemed recommends that each laboratory establish or verify its own reference interval for its patient population.
Lactate Dehydrogenase is present in many tissues, including:
LDH may become elevated in several conditions involving tissue damage or cell turnover. The manufacturer literature mentions associations with myocardial injury, pulmonary disease, hepatic disease, haemolytic anaemia, renal disease and muscular dystrophy.
LDH testing may therefore form part of laboratory evaluation related to:
LDH is not tissue-specific, so results should always be interpreted with other laboratory and clinical findings.
| Related Test | Laboratory Relevance |
|---|---|
| LDH | General tissue enzyme / tissue injury marker |
| AST / SGOT | Liver and muscle enzyme |
| ALT / SGPT | Liver-associated enzyme |
| CK-NAC | Muscle enzyme assessment |
| CK-MB | Cardiac-associated CK fraction |
| Bilirubin | Liver / haemolysis-related assessment |
| Creatinine | Renal profile |
| Urea | Renal profile |
Store the LDH reagents:
The reagents should be brought to the required working temperature before testing and should not be used beyond the labelled expiry date.
The manufacturer notes that the reagents contain sodium azide, which requires appropriate laboratory handling.
Laboratory personnel should:
| Buyer Type | Typical Application |
|---|---|
| Pathology Laboratories | Routine LDH testing |
| Diagnostic Centres | Clinical chemistry enzyme testing |
| Hospital Laboratories | Inpatient and outpatient biochemical testing |
| Cardiac Laboratories | Selected enzyme-profile testing |
| Liver / Gastroenterology Centres | Hepatic enzyme assessment |
| Clinical Chemistry Laboratories | Routine LDH measurement |
| Medical Institutions | Diagnostic and teaching laboratories |
| Healthcare Organisations | Institutional procurement |
| Laboratory Distributors | Professional supply where permitted |
Microsidd India supplies clinical chemistry, cardiac, liver and biochemistry reagents from Bangalore / Bengaluru, Karnataka to pathology laboratories, diagnostic centres, hospitals and institutional healthcare buyers.
The Swemed LDH Reagent Kit 25 ml can be ordered through microsidd.com, subject to stock availability and delivery serviceability.
This product is relevant for buyers searching for:
The Swemed LDH Reagent Kit 25 ml is a professional clinical biochemistry reagent used for quantitative measurement of Lactate Dehydrogenase activity by a kinetic UV method.
The current Swemed product literature identifies the assay as a Modified IFCC kinetic method, while the newer Swemed catalogue describes LDH as an Optimized DGKC pyruvate-to-lactate method.
The assay is measured at 340 nm.
The manufacturer lists serum and heparin plasma.
No. Swemed states that EDTA and sodium citrate may inhibit the assay and should not be used.
The macro procedure uses 25 µl sample.
The stated calculation factor is 8199.
The current product insert states 1000 U/L. The latest Swemed catalogue mentions an updated LDH formulation with linearity up to 1200 IU/L, so laboratories should follow the specification on the current supplied kit.
It can be purchased from Microsidd India through microsidd.com, subject to stock availability.
| Entity | Details |
|---|---|
| Product | LDH Reagent Kit |
| Brand | Swemed Diagnostics |
| Pack Size | 25 ml |
| Analyte | Lactate Dehydrogenase |
| Method | Modified IFCC / Optimized DGKC |
| Reaction | Pyruvate to Lactate |
| Assay Type | UV Kinetic |
| Specimen | Serum / Heparin Plasma |
| Clinical Category | Cardiac / Clinical Chemistry |
| Product Category | Laboratory Reagents |
| Seller | Microsidd India |
| City | Bangalore / Bengaluru |
| State | Karnataka |
| Country | India |
| Website | microsidd.com |
| Buyer Type | Laboratory / Hospital / Diagnostic Centre / B2B |
LDH and other enzyme reagents require careful procurement because laboratories must verify the correct method, wavelength, reagent configuration, pack size, storage requirements, analyser compatibility and seller source before ordering.
Microsidd India has been involved in the IVD and diagnostic-product sector since 2000 and specialises in laboratory, diagnostic and healthcare products. Professional buyers therefore deal with an identifiable specialist seller rather than relying only on an unrelated third-party marketplace listing.
| Buying Factor | Microsidd.com | Large Online Marketplaces | Offline / Local Suppliers |
|---|---|---|---|
| Seller Specialisation | Diagnostic and laboratory focused | Seller expertise varies | Depends on individual dealer |
| Product Knowledge | Better suited to reagent-specific enquiries | Often general marketplace support | Dealer dependent |
| Seller Identity | Direct purchase from Microsidd India | Marketplace and seller may differ | Usually identifiable locally |
| Method Verification | LDH method and pack can be checked before order | Similar listings may cause confusion | Depends on supplier knowledge |
| GST Invoice | Available for eligible purchases | Seller dependent | May vary |
| B2B Procurement | Suitable for laboratories and institutions | Seller dependent | Often local |
| Product Range | Specialist diagnostic and laboratory catalogue | Broad but not specialised | Usually limited by local inventory |
| Customer Support | Direct Microsidd assistance | Marketplace plus seller layer | Local support |
| Accountability | One identifiable specialist supplier | Seller may vary by listing | Depends on supplier |
| India-Wide Access | Subject to serviceability | Broad | Usually regional |
| Microsidd Advantage | Benefit to Laboratory Buyers |
|---|---|
| Diagnostic Product Specialisation | Better suited to professional laboratory procurement |
| IVD Experience Since 2000 | Long-standing diagnostic-sector experience |
| Direct Seller Accountability | Purchase directly from Microsidd India |
| GST Invoice | Useful for laboratories and institutions |
| Product Verification Support | Method, pack and compatibility can be checked before purchase |
| Multi-Category Procurement | Cardiac, liver, renal and other reagents can be sourced together |
| Bangalore-Based Operations | Convenient for Bengaluru and Karnataka laboratories |
| India-Wide Supply | Delivery to serviceable locations across India |
| Direct Customer Support | Product and order-related queries handled directly |
It is intended for quantitative determination of Lactate Dehydrogenase activity in professional clinical chemistry laboratories.
The pack size is 25 ml.
The official product insert states Modified IFCC Method, while Swemed’s newer catalogue describes an Optimized DGKC pyruvate-to-lactate method.
The assay uses 340 nm.
Swemed lists serum and heparin plasma.
No. The manufacturer states that EDTA and sodium citrate may inhibit the assay.
The macro procedure uses 25 µl sample.
Mix 4 parts R1 with 1 part R2.
The current product insert states 1 week at 2–8°C.
The calculation factor is 8199.
The current insert states 1000 U/L.
No. It is intended for professional in vitro diagnostic use only.
The laboratory should verify 340 nm capability, kinetic programming, reaction temperature, sample volume and validated application before ordering.
The product can be ordered from Microsidd India through microsidd.com, subject to stock availability.
The information on this product page is provided for general product identification, procurement and educational purposes only.
This product is intended for professional in vitro diagnostic use by trained laboratory personnel.
LDH is a non-specific tissue enzyme and an elevated or reduced result must not be used independently to diagnose or exclude any particular disease. Interpretation should consider patient history, clinical findings and other relevant laboratory investigations.
The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.
Because Swemed’s current insert and newer catalogue show slightly different performance claims for LDH linearity and working stability, the current package insert supplied with the actual kit should always be followed as the definitive specification.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
For laboratory reagents, customers should specifically verify the test parameter, methodology, analyser compatibility, reagent configuration and pack size before ordering.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
| Specification name | Specification Value |
|---|---|
| Chemical grade | Laboratory, Reagent |
