The J. Mitra HCV TRI-DOT is a rapid, visual and qualitative in-vitro diagnostic test for the detection of antibodies to Hepatitis C Virus (HCV) in human serum or plasma.
The test uses Flow-Through Technology and incorporates purified HCV antigens from Core, NS3, NS4 and NS5 regions. It is designed for rapid professional screening, with results available in less than 3 minutes according to the manufacturer's current information.
Product Name: HCV TRI-DOT Test Kit
Brand: J. Mitra
Manufacturer: J. Mitra & Co. Pvt. Ltd.
Product Category: Rapid Diagnostic Test Kit
Subcategory: HCV / Hepatitis C Test Kit
Test Type: HCV Antibody Screening Test
Target: Antibodies to Hepatitis C Virus (HCV)
Sample Type: Human Serum / Plasma
Test Method: Flow-Through Immunofiltration
Antigens: Core, NS3, NS4 & NS5
Pack Size: 25 Tests
Result Time: Less than 3 minutes
Instrument Required: No dedicated instrument required
Test Format: Rapid Visual Test
Intended Use: In-vitro diagnostic screening
Storage: 2–8°C
SKU: TRIDOT_HCV_25TEST
Suitable Users: Diagnostic laboratories, hospitals, clinics, blood banks and trained healthcare/laboratory personnel
HCV TRI-DOT is a rapid visual test designed for the qualitative detection of antibodies to Hepatitis C Virus (HCV) in human serum or plasma.
J. Mitra describes the product as a 4th Generation HCV TRI-DOT, developed using a combination of modified HCV antigens from the Core, NS3, NS4 and NS5 regions to detect antibodies associated with HCV infection.
The test is intended for professional in-vitro diagnostic use and should be performed by appropriately trained personnel according to the manufacturer's instructions.
The HCV TRI-DOT provides a rapid qualitative screening result in less than 3 minutes according to the manufacturer's current product information.
The test uses Flow-Through Technology, incorporating washing steps during the procedure to help improve test specificity.
The device incorporates highly purified HCV antigens representing:
This antigen combination is designed to support detection of antibodies to HCV.
The manufacturer states that the test is designed for detection of all HCV subtypes.
An integrated Control (C) dot helps validate proper functioning of the device, reagent and procedural application.
The HCV TRI-DOT is a visual rapid test and does not require a dedicated analyser or instrument for reading the result.
HCV antigens are immobilized on a porous immunofiltration membrane.
When the patient's serum or plasma sample passes through the membrane, any HCV antibodies present in the sample can bind to the immobilized HCV antigens.
A subsequent washing step removes unbound proteins. Protein-A conjugate is then added and interacts with captured antibodies, producing visible coloured dots at the test regions.
The device contains a built-in Control (C) dot to indicate proper test functioning.
The test detects antibodies to Hepatitis C Virus in human serum or plasma.
The HCV antigens incorporated into the device represent the Core, NS3, NS4 and NS5 regions of HCV.
Important: HCV TRI-DOT is an antibody screening test. It does not directly detect HCV RNA.
HCV TRI-DOT is intended for qualitative in-vitro diagnostic detection of antibodies to HCV in human serum or plasma.
It is suitable for professional diagnostic environments such as:
The manufacturer states that HCV TRI-DOT has been approved for use in blood banks and has undergone evaluations by WHO and other reference institutions.
HCV TRI-DOT is a screening test.
A reactive result should not by itself be considered confirmation of active HCV infection. Appropriate follow-up and confirmatory testing should be performed according to applicable clinical and laboratory guidelines.
An antibody-based test may also be non-reactive during the early period following infection before detectable antibodies develop.
Therefore, test results should always be interpreted by qualified healthcare or laboratory professionals in conjunction with relevant clinical information.
J. Mitra itself explains that reactive screening results require confirmation using appropriate confirmatory methods.
Pack Size: 25 Tests
The exact components and quantities supplied with each pack should be verified against the current manufacturer packaging and accompanying instructions for use.
The test should be performed strictly according to the manufacturer's current instructions for use.
The general testing process involves:
Do not use generic testing volumes or procedural timings from third-party sources. Always follow the current J. Mitra IFU supplied with the product.
| Specification | Details |
|---|---|
| Product Name | HCV TRI-DOT |
| Brand | J. Mitra |
| Manufacturer | J. Mitra & Co. Pvt. Ltd. |
| Test Parameter | Hepatitis C Virus (HCV) Antibodies |
| Test Type | Rapid Qualitative Antibody Test |
| Generation | 4th Generation |
| Sample Type | Human Serum / Plasma |
| Technology | Flow-Through Technology |
| HCV Antigens | Core, NS3, NS4 & NS5 |
| Pack Size | 25 Tests |
| Result Time | Less than 3 minutes |
| Instrument Required | No dedicated instrument |
| Control | Built-in Quality Control Dot |
| Storage | 2–8°C |
| Product Type | In-Vitro Diagnostic Test |
| SKU | TRIDOT_HCV_25TEST |
The methodology and technical characteristics above are based on J. Mitra's current product information.
J. Mitra reports that HCV TRI-DOT has been evaluated by the World Health Organization (WHO), with reported evaluation performance of 100% sensitivity and 98.9% specificity. The manufacturer also reports evaluations by PATH USA, AIIMS New Delhi and CMC Vellore.
For clarity, these are reported evaluation results under specific evaluation conditions and should not be presented as a blanket guarantee of performance for every specimen, population or testing circumstance.
J. Mitra's current 2026 brochure states a 24-month shelf life at 2–8°C, while the current Microsidd page still states 15 months. Because shelf-life information can change with product versions/batches, the safest customer-facing wording is to rely on the expiry printed on the supplied pack rather than hard-code 15 months.
HCV TRI-DOT is intended for professional diagnostic and laboratory environments.
It may be suitable for:
It is not positioned as a consumer home self-test.
✓ Genuine J. Mitra diagnostic product
✓ Professional HCV antibody screening test
✓ 25-test pack
✓ Rapid visual testing format
✓ Flow-Through Technology
✓ No dedicated analyser required
✓ Suitable for professional laboratory environments
✓ Microsidd India – Bengaluru
✓ Delivery to serviceable locations across India
HCV TRI-DOT is a rapid qualitative in-vitro diagnostic test for detecting antibodies to Hepatitis C Virus in human serum or plasma.
HCV TRI-DOT is manufactured by J. Mitra & Co. Pvt. Ltd., India.
It detects antibodies to Hepatitis C Virus (HCV).
The test is designed for human serum or plasma.
Yes. The manufacturer states that results are available in less than 3 minutes.
No dedicated instrument is required for the visual test.
The test incorporates antigens from Core, NS3, NS4 and NS5 regions of HCV.
J. Mitra states that HCV TRI-DOT is designed for detection of all HCV subtypes.
No. It is a screening test. A reactive result requires appropriate follow-up/confirmatory testing.
Not necessarily. Antibodies may not yet be detectable during the early period following infection. Clinical evaluation and appropriate follow-up testing should be considered when recent exposure is suspected.
No. It is a professional in-vitro diagnostic screening test intended for appropriate laboratory/healthcare use.
Store at 2–8°C, following the manufacturer's instructions and the storage conditions printed on the supplied product packaging.
This Microsidd listing is for a 25-test pack.
J. Mitra HCV TRI-DOT is a professional Anti-HCV antibody screening test designed for qualitative detection of antibodies to Hepatitis C Virus in the specimen types specified by the manufacturer.
The most important interpretation principle is:
Reactive Anti-HCV ≠ Current HCV Infection Confirmed
An Anti-HCV Reactive result indicates that antibodies to Hepatitis C Virus have been detected. This may represent:
Current HCV infection
Previous infection that has resolved
Previously treated HCV infection
Occasionally, a biologically false-reactive antibody result
Therefore, professional HCV screening should connect a Reactive antibody result to the appropriate HCV RNA testing pathway.
The next key question is whether HCV RNA is detectable.
Anti-HCV Reactive
→ HCV RNA / NAT
HCV RNA Detected
→ Current HCV infection is present
HCV RNA Not Detected
→ No current HCV infection in most situations
This distinction is essential because HCV antibodies may remain detectable even after spontaneous viral clearance or successful treatment.
HCV Rapid Test Kit Complete Guide
Learn how HCV antibody rapid tests work, what Reactive and Non-Reactive results mean, testing limitations and the role of professional follow-up.
HCV Antibody vs HCV RNA: Understanding Screening, Current Infection & Follow-Up
Understand why Anti-HCV antibody testing identifies exposure while HCV RNA testing determines whether current infection is present.
HCV Testing During Pregnancy & Mother-to-Child Transmission
Learn how maternal Anti-HCV and HCV RNA results are interpreted during pregnancy and why a Reactive maternal antibody result does not automatically establish current maternal infection.
Rapid Diagnostic Tests for Gynecology & Antenatal Clinics: Pregnancy, HIV, HBsAg, HCV & Syphilis
This professional guide connects pregnancy testing, HIV, HBsAg, HCV and syphilis screening with appropriate clinic workflow, interpretation and referral.
Rapid Test Do’s & Don’ts: HIV, HBsAg, HCV & Syphilis Sample Handling, Storage & Common Errors
Review specimen handling, kit storage, expiry checks, manufacturer-specific procedures, result timing, controls, documentation and common testing errors.
Professional-use note: HCV TRI-DOT detects Anti-HCV antibodies; it does not directly detect HCV RNA. A Reactive result should enter the appropriate diagnostic pathway for assessment of current infection. Always follow the exact current J. Mitra Instructions for Use for specimen requirements, reagent volumes, timings, storage and interpretation.
This product is non-returnable and non-exchangeable.
Please verify the brand, model, compatibility, pack size and product specifications before placing your order.
Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com's applicable policies and applicable law.
An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.
This product is an in-vitro diagnostic screening test intended for professional use.
HCV TRI-DOT detects antibodies to HCV and should not by itself be used to establish active HCV infection. Reactive results should be appropriately evaluated and confirmed according to applicable medical and laboratory guidelines.
This product does not replace professional medical advice, diagnosis or clinical evaluation.
| Specification name | Specification Value |
|---|---|
| Test method | At-home test (self-administered), Lab analysis, RDT |
| Test sample | Blood |
