Dengue NS1 Ag Elisa

DENGUE NS1 ELISA KIT

INTENDED USE

DENGUE NS1 Ag MICROLISA is designed for in vitro qualitative detection of Dengue NS1 antigen in human serum or plasma and is used as a screening test for testing of collected blood samples suspected for DENGUE. The kit detects all four subtypes; DEN1, DEN2, DEN3 & DEN4 of Dengue Virus.

SALIENT FEATURES

DENGUE NS1 Ag MICROLISA is a solid phase enzyme linked immunosorbent assay (ELISA) based on the "Direct Sandwich" principle. The microwells are coated with Anti-Dengue NS1 antibodies with high reactivity for Dengue NS1 antigen. The samples are added in the wells followed by addition of enzyme conjugate (monoclonal anti-dengue NS1 antibodies linked to Horseradish peroxidase (HPRO)). A sandwich complex is formed in the well wherein dengue NS1 (from serum sample) is ‘’trapped’’ or ‘’sandwiched’’ between the antibody and antibody HRPO conjugate. Unbound conjugate is then washed off with wash buffer. The amount of bound peroxidase is proportional to the concentration of dengue NS1 antigen present in the sample. Upon addition of the substrate buffer and chromogen, a blue colour develops. The intensity of developed blue colour is proportional to the concentration of dengue NS1 antigen in sample. To limit the enzyme-substrate reaction, stop solution is added and a yellow colour develops which is finally read at 450 nm spectrophotometrically.

ORDERING INFORMATION

PERFORMANCE CHARACTERISTICS (IN HOUSE EVALUATION) OF DENGUE NS1 Ag MICROLISA 

Sensitivity- 99.50% Specificity-100.00 %