DENGUE IgG Microlisa test is an enzyme immunoassay based on "Capture ELISA". Anti-human IgG antibodies are coated onto microtiter wells. Specimens and controls are added to the microtiter wells and incubated. Antibodies to Dengue if present in the specimen, will bind to the Anti-human IgG antibodies adsorbed onto the surface of the wells. The plate is then washed to remove unbound material. Horseradish peroxidase (HRPO) conjugated dengue antigen (DEN-4) is added to each well. This dengue antigen conjugate will bind to Dengue specific IgG antibodies which is complexed with anti human IgG antibodies. Finally substrate solution containing chromogen and hydrogen peroxide is added to the wells and incubated. A blue colour will develop in proportion to the amount of dengue antibodies present in the specimen. The colour reaction is stopped by a stop solution. The enzyme substrate reaction is read by EIA reader for absorbance at a wavelength of 450 nm. If the sample does not contain Dengue IgG antibodies then enzyme conjugate will not bind and the solution in the wells will be either colourless or only a faint background colour develops.
| No. of Samples | Status | DENGUE IgG MICROLISA | ||
| Positive | Equivocal | Negative | ||
| 75 | Dengue Positive | 74 | 1 | 0 |
| 5740 | Dengue Negative | 2 | 2 | 5736 |
PERFORMANCE CHARACTERISTICS (IN HOUSE EVALUATION) OF DENGUE IgG ELISA
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