Biolab Rapid Thalassemia is a professional NESTROFT – Naked Eye Single Tube Red Cell Osmotic Fragility Test designed as a screening aid for thalassemia carrier detection.
The test compares red-cell haemolysis in a control tube and a low-osmolality test reagent. Red cells associated with thalassemia carrier states may show increased resistance to osmotic lysis, producing a relatively less transparent test suspension.
This listing is for the 50-test pack.
Brand: Biolab Diagnostics
Product: Rapid Thalassemia – NESTROFT Reagent
Catalogue No.: BC 250
Pack Size: 50 Tests
Method: Naked Eye Single Tube Red Cell Osmotic Fragility Test
Result Type: Qualitative visual screening
Intended User: Professional laboratory personnel
Biolab Rapid Thalassemia NESTROFT is intended as a screening tool for thalassemia carrier detection in professional laboratory settings.
Typical applications include:
The test should not be used as a standalone confirmatory diagnosis of beta-thalassemia trait.
NESTROFT is based on the osmotic fragility behaviour of red blood cells.
According to Biolab, red cells associated with elevated HbA2 / thalassemia carrier patterns show a reduced tendency to lyse in a low-molarity buffered sodium chloride solution.
As a result:
The test result is visually compared against the control using the supplied Rapid Thalassemia Reader.
For the 50-test configuration, Biolab's literature lists:
The 100 supplied tubes support the control-and-test format, with two tubes used per patient test.
Biolab describes the reagents as ready to use.
The manufacturer states that no prior patient preparation is required.
Suitable samples include:
Biolab also notes that accuracy may improve when testing packed red cells obtained after allowing the blood sample to stand and collecting RBC sediment from the bottom.
Samples may be stored for approximately one week at 4–8°C when testing is delayed, according to the manufacturer literature.
Follow the current Biolab IFU supplied with the actual kit.
The NESTROFT workflow uses two tubes:
The exact reagent and sample volumes should follow the current lot-specific IFU because the procedure diagram in Biolab's scanned literature is not fully legible in text extraction.
The TEST tube appears relatively transparent, similar to or clearer than the control pattern according to the manufacturer interpretation guide.
The TEST tube remains relatively less transparent / more turbid than the CTRL tube when viewed in the Rapid Thalassemia Reader.
This reflects reduced osmotic lysis of the red cells.
A positive or doubtful NESTROFT result must be confirmed by an appropriate confirmatory method such as Hb electrophoresis or another validated haemoglobin-analysis technique.
In the study reported in Biolab's IFU, the manufacturer lists the following performance:
These values relate to the manufacturer-reported study and should not be interpreted as guaranteed performance in every population or laboratory setting.
Biolab recommends that testing be performed with known:
Controls should preferably have a similar haemoglobin concentration to the patient samples.
Each laboratory should establish its own QC acceptance criteria and corrective-action procedure.
NESTROFT is a screening test and not a definitive haemoglobinopathy diagnostic method.
Possible limitations include:
Clinical decisions should not be based solely on NESTROFT.
| Feature | Biolab NESTROFT | Hb Electrophoresis / HPLC |
|---|---|---|
| Purpose | Initial screening | Confirmatory / haemoglobin characterisation |
| Result | Qualitative | Analytical / more specific |
| Equipment | Simple reader / tubes | Dedicated instrument required |
| Use in Population Screening | Well suited as preliminary screening | Generally used for confirmation / detailed analysis |
| Final Diagnosis | No | Used as part of confirmatory evaluation |
According to the Biolab IFU:
The actual current pack label and IFU take precedence over any online description.
| Product Name | Rapid Thalassemia – NESTROFT Reagent |
| Brand | Biolab Diagnostics |
| Catalogue No. | BC 250 |
| Pack Size | 50 Tests |
| Method | NESTROFT / Naked Eye Single Tube Red Cell Osmotic Fragility Test |
| Specimen | Fresh Finger-Prick Blood / EDTA Whole Blood |
| Result Type | Qualitative Visual Result |
| Interpretation Time | Approx. 30 Minutes |
| R1 Control | 100 mL |
| R2 Test Reagent | 100 mL |
| Test Tubes + Caps | 100 Nos. for control + test tubes |
| Reader | Rapid Thalassemia Reader Included |
| Storage | Room Temperature, Protected from Light; Do Not Freeze |
| Intended User | Professional Laboratory Personnel |
Thalassemia screening products can differ in method, reagent concentration, pack size, accessory configuration and interpretation procedure.
Microsidd clearly identifies this product as Biolab Rapid Thalassemia NESTROFT Reagent – BC 250 – 50 Tests.
| Buying Consideration | Microsidd India | General Online Marketplace | Typical Offline Store |
|---|---|---|---|
| Exact Method | NESTROFT clearly identified | May simply say “thalassemia test” | Depends on staff expertise |
| Pack Size | 50 tests clearly stated | Seller dependent | Depends on local stock |
| Accessories | Control, test reagent, tubes, droppers & reader explained | May not be fully described | Requires physical inspection |
| Clinical Limitation | Confirmatory Hb testing clearly recommended | Listing quality varies | Dependent on staff guidance |
| GST Invoice | Available for eligible orders | Seller dependent | Store dependent |
| Bengaluru Presence | RT Nagar, Bengaluru | Seller may be elsewhere | Depends on local supplier |
| India-Wide Ordering | Available to serviceable locations | Platform dependent | Usually local purchase |
Microsidd India supplies haematology reagents, thalassemia screening products, laboratory diagnostic kits and related professional-use consumables from RT Nagar, Bengaluru.
Customers searching for NESTROFT reagent Bangalore, thalassemia screening kit Bengaluru, Biolab Rapid Thalassemia near me, osmotic fragility test RT Nagar, thalassemia test reagent Bangalore or Biolab Diagnostics supplier Karnataka can check current availability through Microsidd.com.
Laboratories and screening programmes requiring multiple kits should confirm current stock, batch and expiry before ordering.
Business Name: Microsidd India
Address: #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India
Phone / WhatsApp: +91 63667 72288
Email: support@microsidd.com
Website: https://microsidd.com
Working Hours: Monday to Saturday, 10:00 AM – 6:00 PM IST
View Microsidd India on Google Maps
For Bengaluru ordering information, visit Microsidd India – Medical & Laboratory Supplies Bangalore.
NESTROFT stands for Naked Eye Single Tube Red Cell Osmotic Fragility Test.
It is used as a screening test to identify samples that may be associated with thalassemia carrier states.
This listing is for 50 tests.
Biolab lists the 50-test product as BC 250.
Fresh finger-prick blood or EDTA whole blood can be used according to the Biolab IFU.
No special prior patient preparation is specified by Biolab.
The result is observed approximately 30 minutes after sample mixing.
The TEST tube is compared visually with the CTRL tube using the Rapid Thalassemia Reader.
The TEST tube appears relatively less transparent / more turbid than the control.
No. Positive and doubtful results require confirmation using Hb electrophoresis, HPLC or another validated haemoglobin-analysis method.
No. It is a screening tool and does not provide definitive haemoglobin variant identification.
The system includes a Control Reagent R1 and Test Reagent R2.
Yes. The manufacturer literature lists 100 test tubes with caps for the 50-test control-and-test configuration.
Yes. One Rapid Thalassemia Reader is supplied.
Store at room temperature, protect from light, avoid contamination and do not freeze.
Biolab states that samples may be stored for approximately one week at 4–8°C if required.
Yes. Biolab recommends known positive and negative blood controls.
No. It is intended for trained professional laboratory personnel.
Biolab Rapid Thalassemia NESTROFT reagent can be ordered through Microsidd India, RT Nagar, Bengaluru, subject to stock availability.
Yes. Microsidd supplies professional laboratory and diagnostic products to serviceable locations across India.
Biolab Rapid Thalassemia NESTROFT is a professional screening test and should be performed only by trained laboratory personnel according to the current manufacturer IFU and laboratory SOP.
NESTROFT does not independently diagnose thalassemia or identify a specific haemoglobin variant.
Results may be influenced by:
Positive or doubtful screening results should be confirmed with an appropriate haemoglobin-analysis method such as electrophoresis or HPLC.
The information on this page is intended for product identification, procurement and educational reference and does not replace Biolab's official IFU, laboratory SOPs or professional medical interpretation.
This is a professional diagnostic reagent and method-sensitive laboratory consumable.
Please verify before ordering:
A correctly supplied kit cannot normally be returned or exchanged because the wrong method, reagent configuration, pack size or laboratory requirement was selected.
Laboratory reagents and diagnostic consumables are expiry-sensitive, storage-sensitive and contamination-sensitive and cannot normally be returned to saleable inventory once correctly supplied.
Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the Microsidd Refund, Return, Replacement & Cancellation Policy.
An original, continuous, unedited and uncut unboxing video is compulsory for claims involving an incorrect, missing or damaged product.
Begin recording before opening the parcel. Open the outer carton carefully by cutting the sealing tape with a blade or suitable cutter; do not tear the carton. Keep the courier label, sealed Biolab kit, reagents, tubes, reader, pack size, batch and expiry continuously visible.
Product: Rapid Thalassemia NESTROFT Reagent
Brand: Biolab Diagnostics
Catalogue No.: BC 250
Pack Size: 50 Tests
Method: Naked Eye Single Tube Red Cell Osmotic Fragility Test
Specimen: Fresh Finger-Prick / EDTA Whole Blood
Result: Qualitative Visual Transparency / Turbidity Comparison
Interpretation Time: Approx. 30 Minutes
Reagents: R1 Control 100 mL + R2 Test Reagent 100 mL
Accessories: Test Tubes, Caps, Droppers & Rapid Thalassemia Reader
Confirmation: Positive / Doubtful Results Require Hb Electrophoresis or Equivalent Confirmatory Testing
Storage: Room Temperature, Protected from Light, Do Not Freeze
Intended User: Professional Laboratory Personnel
Seller: Microsidd India
Seller Location: RT Nagar, Bengaluru, Karnataka, India
| Specification name | Specification Value |
|---|---|
| Test sample | Blood |
