Biolab Rapid Malaria Stain Kit (JSB I & II) is a professional laboratory staining system designed for rapid staining of peripheral blood smears for microscopic demonstration of malaria parasites and blood-cell morphology.
The system uses polychromated Methylene Blue and Eosin to differentially stain basophilic and acidophilic cellular components, helping laboratory professionals visualise blood cells and haemoparasites during microscopy.
The principal stain pack contains:
The Biolab manufacturer insert also lists an Easyfix Peripheral Smear Fixative – 50 mL within the staining system. Verify the exact contents of the current supplied pack before ordering if the fixative is specifically required.
Brand: Biolab Diagnostics
Product: Rapid Malaria Stain Kit – JSB I & II
Pack Size: 2 × 500 mL main stain reagents
Method: Rapid JSB Blood Parasite Staining
Application: Malaria Parasite Microscopy
Intended User: Professional Laboratory Personnel
Biolab Rapid Malaria Stain is primarily designed for staining peripheral blood smears for microscopic examination of malaria parasites.
Professional applications include:
The manufacturer insert specifically describes microscopic appearance for malaria parasites including Plasmodium vivax trophozoites and Plasmodium falciparum trophozoites / gametocytes.
The staining principle is based on the differential affinity of polychromated Methylene Blue and Eosin for cellular structures.
Biolab states that the stains react specifically with:
This produces colour contrast that allows malaria parasites and blood-cell structures to be visualised microscopically.
The manufacturer insert lists:
The manufacturer insert lists:
The Biolab insert also lists Easyfix Peripheral Smear Fixative – 50 mL.
Because commercial pack configurations can change, laboratories requiring the fixative should verify the current sealed-pack contents before ordering.
Biolab specifies that the reagents are ready to use.
No dilution or working-reagent preparation is described for the JSB I and JSB II stains.
This simplifies routine laboratory staining and helps reduce reagent-preparation variability.
The manufacturer insert describes the use of peripheral blood samples prepared on clean glass slides.
Blood samples may be obtained during febrile episodes when malaria is clinically suspected.
Biolab also states that EDTA-anticoagulated blood may be used without substantial change in staining results.
A thin peripheral blood film may be prepared by spreading a small drop of blood with a fine-edged spreader.
The smear should be:
Fixed smears may be stored for a longer period, although Biolab recommends staining as soon as reasonably possible.
According to the current manufacturer insert:
Biolab notes that staining time may be increased or decreased depending on the individual staining result.
Thick smears are useful where a greater volume of blood is examined microscopically.
The Biolab procedure includes a dehaemoglobinisation step for thick smears before staining.
Always follow the current pack insert supplied with the actual reagent lot, particularly for smear fixation and thick-smear processing.
| Structure | Manufacturer-Described Appearance |
|---|---|
| Leucocyte Nuclei | Varying shades of dark blue |
| Leucocyte Cytoplasm | Pale or light blue |
| Red Blood Cells | Pale reddish |
| Malaria Parasites | Varying shades of blue |
| Schüffner's Dots | Dark bluish-red |
| Platelets | Pale or dark blue |
The Biolab IFU also describes bulk staining using staining tanks / slide holders.
This may be useful for:
The exact bulk-staining sequence and timings should follow the current Biolab pack insert.
The manufacturer insert states that the reagents are stable when stored at approximately 25–35°C for up to 60 months, subject to the expiry printed on the actual supplied product.
Important storage precautions include:
Biolab also notes that JSB stain may become exhausted after prolonged use, particularly after staining a large number of smears.
If staining becomes progressively lighter, the reagent should be replaced according to laboratory QC practice.
The manufacturer recommends periodically checking stain performance using:
Laboratories should maintain appropriate staining controls and documented QC procedures.
Stain performance should be reviewed whenever:
Microscopic malaria examination depends on more than the staining reagent alone.
The Biolab IFU specifically notes that accuracy also depends on:
A negative smear does not always exclude malaria, particularly when parasitaemia is low or specimen timing / preparation is suboptimal.
| Product Name | Rapid Malaria Stain Kit JSB I & II |
| Brand | Biolab Diagnostics |
| Main Pack Size | 2 × 500 mL |
| JSB I | 500 mL |
| JSB II | 500 mL |
| Fixative Listed in IFU | Easyfix Peripheral Smear Fixative – 50 mL; verify current supplied pack |
| Method | Rapid JSB Differential Staining |
| Primary Application | Malaria Parasite / Haemoparasite Microscopy |
| Specimen | Peripheral Blood Smear / EDTA Blood |
| Thin Smear JSB II | Approx. 3–5 Seconds |
| Thin Smear JSB I | Approx. 35–45 Seconds |
| Thick Smear JSB II | Approx. 5–6 Seconds |
| Thick Smear JSB I | Approx. 35–45 Seconds |
| Microscopy | Oil Immersion |
| Working Reagent | Ready to Use |
| Storage | Manufacturer IFU states approximately 25–35°C; follow actual pack label |
| Stability | Up to approximately 60 Months according to IFU; verify actual expiry |
| Intended User | Professional Laboratory Personnel |
| Feature | Biolab Rapid JSB I & II | Conventional Longer Staining Method |
|---|---|---|
| Workflow | Rapid stain sequence | Usually longer stain contact time |
| Thin Smear | Supported | Supported depending on method |
| Thick Smear | Supported | Supported depending on method |
| Reagent Preparation | Ready to use | Method dependent |
| Best Use | Rapid routine microscopy | Depends on laboratory SOP |
Malaria staining reagents can differ significantly in method, stain composition, staining sequence, timing, pack size and smear compatibility.
Microsidd clearly identifies this product as Biolab Rapid Malaria Stain Kit – JSB I & II – 2 × 500 mL.
| Buying Factor | Microsidd India | General Online Marketplace | Typical Offline Store |
|---|---|---|---|
| Exact Method | JSB I & II clearly stated | May be listed simply as malaria stain | Depends on staff familiarity |
| Pack Size | 2 × 500 mL clearly stated | May differ by seller | Depends on local stock |
| Procedure Information | Thin- and thick-smear applications explained | Listing detail varies | Usually dependent on staff knowledge |
| IFU Verification | Manufacturer instructions emphasised | Seller dependent | Store dependent |
| GST Invoice | Available for eligible orders | Seller dependent | Store dependent |
| Bengaluru Presence | RT Nagar, Bengaluru | Seller may be located elsewhere | Depends on local availability |
| India-Wide Ordering | Available to serviceable locations | Platform dependent | Usually local purchase |
Microsidd India supplies laboratory stains, malaria microscopy reagents, diagnostic reagents and professional laboratory consumables from RT Nagar, Bengaluru.
Customers searching for malaria stain kit Bangalore, JSB stain Bengaluru, Biolab Rapid Malaria Stain near me, blood parasite stain RT Nagar, malaria microscopy stain Bangalore, JSB I & II supplier Karnataka or Biolab Diagnostics supplier Bengaluru can check current availability through Microsidd.com.
Laboratories and institutions requiring multiple bottles should confirm current stock, batch and expiry before ordering.
Business Name: Microsidd India
Address: #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India
Phone / WhatsApp: +91 63667 72288
Email: support@microsidd.com
Website: https://microsidd.com
Working Hours: Monday to Saturday, 10:00 AM – 6:00 PM IST
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This listing is for Biolab Rapid Malaria Stain Kit – JSB I & II.
The main stain pack contains JSB I 500 mL + JSB II 500 mL.
The supplied Biolab IFU lists an Easyfix Peripheral Smear Fixative 50 mL. Because pack configurations may change, confirm the actual current commercial pack if the fixative is specifically required.
It is designed for rapid staining of peripheral blood smears for microscopic demonstration of malaria parasites and other blood-cell structures.
Yes. Biolab provides a dedicated thin-smear staining procedure.
Yes. The IFU also provides a procedure for dehaemoglobinised thick smears.
No. Biolab describes the JSB reagents as ready to use.
The system is based primarily on polychromated Methylene Blue and Eosin staining.
The manufacturer procedure uses approximately 3–5 seconds in JSB II followed by approximately 35–45 seconds in JSB I, excluding fixation, washing and drying.
The principal stain-contact steps can be completed rapidly, with the combined stain exposure around one minute or less under the manufacturer's procedure. Total workflow time also includes fixation, washing, drying and microscopy.
The Biolab method includes dehaemoglobinisation with distilled water before the rapid staining sequence.
Biolab describes malaria parasites as appearing in varying shades of blue under the specified stain.
The manufacturer describes red blood cells as pale reddish.
They are described as varying shades of dark blue.
Yes. Biolab states that EDTA-anticoagulated blood may be used without substantial change in staining results.
The stained smear is examined using a suitable laboratory microscope under oil immersion according to the manufacturer procedure.
Biolab recommends periodic checking using known positive and negative samples.
The supplied IFU states approximately 25–35°C. Keep reagents appropriately capped and follow the actual storage instructions printed on the current pack.
The manufacturer insert states up to approximately 60 months under prescribed conditions. Always use the printed expiry date on the supplied batch as the final reference.
No. Biolab advises replacing the stain if prolonged use produces progressively lighter staining.
No. Microscopy sensitivity depends on parasite density, specimen timing, smear quality, staining and examiner expertise. Clinical evaluation and repeat / alternative testing may be required.
No. This product is intended for trained professional laboratory personnel.
Biolab Rapid Malaria Stain JSB I & II can be ordered through Microsidd India in RT Nagar, Bengaluru, subject to current stock and batch availability.
Yes. Microsidd supplies professional laboratory products to serviceable locations across India, subject to stock and courier serviceability.
Biolab Rapid Malaria Stain JSB I & II is a professional laboratory staining reagent and should be used only by trained personnel according to the current manufacturer IFU, laboratory SOP, biosafety procedures and microscopy QC requirements.
A stained blood smear is one component of malaria laboratory investigation.
Result quality may be affected by:
A negative microscopy result does not automatically exclude malaria where clinical suspicion remains high.
Diagnostic interpretation and subsequent patient management should be performed by appropriately qualified healthcare and laboratory professionals.
This is a professional laboratory reagent and is method-, application-, storage- and expiry-sensitive.
Please verify before ordering:
A correctly supplied reagent cannot normally be returned or exchanged because a different staining method, pack configuration, application or laboratory SOP was required.
Laboratory reagents are storage-sensitive, expiry-sensitive and contamination-sensitive products and cannot normally be returned to saleable stock once correctly delivered.
Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the Microsidd Refund, Return, Replacement & Cancellation Policy.
An original, continuous, unedited and uncut unboxing video is compulsory for delivery-related claims involving an incorrect, missing or damaged product.
Begin recording before opening the parcel. Open the outer carton carefully by cutting the sealing tape with a blade or suitable cutter; do not tear the carton. Keep the courier label, sealed Biolab packaging, reagent bottles, pack configuration, batch and expiry continuously visible.
Product: Rapid Malaria Stain Kit
Brand: Biolab Diagnostics
Method: JSB I & II Rapid Differential Staining
Pack: JSB I 500 mL + JSB II 500 mL
IFU-Listed Fixative: Easyfix Peripheral Smear Fixative 50 mL; verify current supplied configuration
Primary Application: Malaria Parasite Microscopy
Specimen: Peripheral Blood Smears / EDTA Blood
Smear Types: Thin & Thick Blood Smears
Main Stain Chemistry: Polychromated Methylene Blue & Eosin
Preparation: Ready to Use
Microscopy: Oil Immersion
Storage: Manufacturer IFU states approximately 25–35°C; verify current pack
Intended User: Professional Laboratory Personnel
Seller: Microsidd India
Seller Location: RT Nagar, Bengaluru, Karnataka, India
| Specification name | Specification Value |
|---|---|
| Chemical grade | Laboratory |
| Color pattern | White |
