Biolab Rapid AFB Stain Kit (Cold Method) is a professional bacteriology staining reagent kit designed for Acid-Fast Bacilli (AFB) staining in microbiology, pathology and diagnostic laboratory workflows.
The system uses a cold staining method, meaning the procedure does not require the heating step associated with conventional hot Ziehl-Neelsen staining.
The kit contains two ready-to-use staining reagents:
Brand: Biolab Diagnostics
Product: Rapid AFB Stain Kit
Method: Cold Method / No Heating
Pack Size: 2 × 100 mL
Application: Professional AFB / Mycobacterial Microscopy
AFB staining is a microscopy staining technique used to help identify organisms that retain certain dyes even after exposure to an acidic decolourising solution.
These organisms are referred to as acid-fast bacilli.
The technique is commonly used in professional microbiology laboratories as part of the microscopic evaluation of specimens where acid-fast organisms are being investigated.
Important: an AFB stain is a laboratory screening / microscopy aid and does not by itself establish a final microbiological diagnosis.
A key advantage of the Biolab Rapid AFB system is the cold staining method.
Conventional Ziehl-Neelsen techniques may involve heating during primary staining. Biolab's Rapid AFB system is formulated to support dye penetration without that heating step.
This can help laboratories:
The complete staining procedure and timing must follow the current Biolab IFU supplied with the kit.
According to the manufacturer's product information, the primary red stain contains a pararosaniline-based dye system with phenol and detergent.
The detergent assists stain penetration into organisms that are otherwise difficult to stain.
Acid-fast bacilli retain the red dye during subsequent decolourisation / counterstaining, while the background develops a contrasting green colour.
Under appropriate microscopy conditions, acid-fast organisms may therefore appear red to pink against a greenish background.
Interpretation must always follow the laboratory's validated microscopy procedure and the current manufacturer IFU.
| Component | Pack | Role |
|---|---|---|
| Rapid-AFB Red Stain | 100 mL | Primary AFB staining reagent |
| Rapid-AFB Green Stain | 100 mL | Combined counterstaining / decolourising system according to manufacturer method |
The Biolab manufacturer listing describes the reagents as follows:
Both stains are described by the manufacturer as ready to use.
The current lot label and package insert should be treated as the definitive formulation reference.
| Feature | Biolab Rapid AFB Cold Method | Conventional Heated ZN Method |
|---|---|---|
| Primary Method | Cold method | Heated staining procedure |
| Heating | Not required | Commonly required |
| Primary Stain | Rapid-AFB Red Stain | Carbol fuchsin-based stain |
| Counterstain | Rapid-AFB Green Stain system | Method dependent |
| Workflow | Designed for rapid routine workflow | Traditional multi-step workflow |
The laboratory should use the method specified by its validated SOP, accreditation requirements and manufacturer documentation.
The exact staining sequence, contact times, washing steps and microscopy procedure must follow the current Biolab package insert.
A general professional workflow involves:
No heating should be added unless specifically directed by the current manufacturer IFU.
Under the manufacturer-described staining principle:
Actual microscopic interpretation depends on:
The Biolab Rapid AFB Stain Kit may be used in professional settings such as:
Use should be restricted to trained laboratory personnel following the laboratory SOP and current manufacturer instructions.
Biolab's manufacturer listing states that the Rapid AFB reagents are stable for approximately 24 months when stored correctly.
Recommended handling from the manufacturer listing includes:
A separate manufacturer-distributor listing describes the product as suitable for room-temperature storage.
The storage conditions printed on the current supplied Biolab pack / IFU take precedence.
| Product Name | Rapid AFB Stain Kit |
| Brand | Biolab Diagnostics |
| Method | Cold Method |
| Heating Required | No |
| Pack Size | 2 × 100 mL |
| Red Stain | 100 mL |
| Green Stain | 100 mL |
| Form | Ready-to-Use Liquid Reagents |
| Primary Application | AFB / Mycobacterial Microscopy Staining |
| Stability | Approx. 24 Months according to manufacturer listing; verify current pack expiry |
| Intended User | Professional Laboratory Personnel |
AFB staining reagents can differ in method, composition, pack configuration, heating requirement and staining procedure. Microsidd clearly identifies this listing as the Biolab Rapid AFB Stain Kit – Cold Method – 2 × 100 mL.
| Buying Factor | Microsidd India | General Online Marketplace | Typical Offline Store |
|---|---|---|---|
| Exact Method | Cold Method clearly stated | May be omitted | Depends on staff knowledge |
| Pack Configuration | 2 × 100 mL clearly identified | May vary by seller | Depends on available stock |
| Reagent Components | Red 100 mL + Green 100 mL explained | Often not fully described | Requires pack inspection |
| Technical Guidance | Method, storage and IFU verification highlighted | Listing dependent | Store dependent |
| GST Invoice | Available for eligible purchases | Seller dependent | Store dependent |
| Bengaluru Presence | RT Nagar, Bengaluru | Seller may be located elsewhere | Depends on local availability |
| India-Wide Ordering | Available to serviceable PIN codes | Platform / seller dependent | Usually local purchase only |
Microsidd India supplies laboratory reagents, bacteriology stains, diagnostic kits and laboratory consumables from RT Nagar, Bengaluru.
Customers searching for Rapid AFB stain kit Bangalore, Biolab AFB stain Bengaluru, AFB cold method stain near me, mycobacteria stain RT Nagar, bacteriology stains Bangalore or Biolab Diagnostics supplier Karnataka can check current stock through Microsidd.com.
Laboratories requiring multiple packs or institutional quantities should confirm current batch, expiry and availability before ordering.
Business Name: Microsidd India
Address: #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India
Phone / WhatsApp: +91 63667 72288
Email: support@microsidd.com
Website: https://microsidd.com
Working Hours: Monday to Saturday, 10:00 AM – 6:00 PM IST
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Browse related products in Microsidd Laboratory Needs.
For Bengaluru ordering information, visit Microsidd India – Laboratory & Medical Supplies Bangalore.
The kit contains 2 × 100 mL bottles.
The manufacturer lists a Rapid-AFB Red Stain 100 mL and Rapid-AFB Green Stain 100 mL.
Yes. Biolab identifies the product as a Cold Method Rapid AFB Stain Kit.
No. The manufacturer describes the Rapid AFB method as no heating required.
It is intended for professional staining of smears for microscopic evaluation of acid-fast bacilli / mycobacteria.
No. A staining result is one laboratory finding and should not independently establish or exclude a clinical diagnosis.
Under the manufacturer's staining principle, acid-fast bacilli retain the red stain and may appear deep red to light red / pink against a greenish background.
Yes. Biolab describes both stain solutions as ready to use.
The manufacturer listing states approximately 24 months. Always follow the expiry printed on the actual supplied kit.
Biolab states that bottles should remain tightly capped and protected from bright light. A manufacturer-distributor listing describes room-temperature storage. Follow the current product label / IFU for the supplied batch.
Do not refrigerate unless the current Biolab label or IFU instructs you to do so. Follow the storage conditions printed on the actual supplied pack.
Specimen collection and preparation must follow the laboratory's validated AFB microscopy SOP and current manufacturer instructions. Microsidd does not recommend relying on generic ecommerce specimen descriptions.
Use the exact contact times stated in the current Biolab Rapid AFB package insert. Do not substitute timings from another AFB staining kit.
The manufacturer listing describes the method as compatible with many automatic staining systems, but the exact instrument and protocol should be validated by the laboratory before routine use.
For validated performance, use the complete Biolab Rapid AFB system unless your laboratory has formally validated an alternative reagent combination.
No. This product is intended for trained professional laboratory personnel.
No. Do not use reagents after the manufacturer-printed expiry date.
Biolab Rapid AFB Stain Kit can be ordered through Microsidd India, RT Nagar, Bengaluru, subject to current stock and batch availability.
Yes. Microsidd supplies laboratory and diagnostic products to serviceable locations across India, subject to stock and courier availability.
Biolab Rapid AFB Stain Kit is a professional laboratory staining reagent and should be used only by trained personnel according to the current manufacturer IFU and laboratory SOP.
An AFB microscopy result should not be interpreted in isolation.
Result quality may be affected by:
Clinical and microbiological decisions should consider other laboratory findings, clinical information and confirmatory investigations where appropriate.
Information on this page is intended for product identification and professional procurement and does not replace the Biolab package insert, laboratory SOP, biosafety procedures or professional interpretation.
This is a professional laboratory reagent and method-sensitive diagnostic product.
Please verify before ordering:
A correctly supplied kit cannot normally be returned or exchanged because the wrong staining method, reagent configuration or laboratory requirement was selected.
Laboratory reagents are expiry-sensitive, contamination-sensitive and storage-sensitive products and cannot normally be returned to saleable inventory once correctly supplied.
Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the Microsidd Refund, Return, Replacement & Cancellation Policy.
An original, continuous, unedited and uncut unboxing video is compulsory for claims involving an incorrect, missing or damaged product.
Begin recording before opening the shipment. Open the outer carton carefully by cutting the sealing tape with a blade or suitable cutter; do not tear the carton. Keep the courier label, sealed Biolab box, reagent bottles, batch, expiry and pack configuration continuously visible.
Product: Rapid AFB Stain Kit
Brand: Biolab Diagnostics
Method: Cold Method
Heating: Not Required
Pack Size: 2 × 100 mL
Components: Rapid-AFB Red Stain 100 mL + Rapid-AFB Green Stain 100 mL
Format: Ready-to-Use Liquid Reagents
Primary Application: Acid-Fast Bacilli / Mycobacterial Microscopy Staining
Stability: Approx. 24 Months according to manufacturer listing; verify current batch
Storage: Tight Capped, Protected from Bright Light; follow current label
Intended User: Professional Laboratory Personnel
Seller: Microsidd India
Seller Location: RT Nagar, Bengaluru, Karnataka, India
