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Benedict’s Reagent Solution for Reducing Sugar Test | 500ml & 5L

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SKU: benedict's reagent Solution 500ml
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Benedicts solution reagent
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Benedict’s Reagent Solution for Reducing Sugar Testing – 500ml & 5L

Benedict’s Reagent Solution, also known as Benedict’s Solution or Benedict’s qualitative reagent, is a laboratory reagent used for the qualitative detection of reducing sugars in suitable laboratory samples.

The reagent contains a combination of copper sulphate, sodium citrate and sodium carbonate. When used under appropriate heated alkaline conditions, reducing sugars can reduce copper(II) ions and produce a visible colour change and copper(I) oxide precipitate.

Microsidd India supplies Benedict’s Reagent Solution in 500ml and 5000ml (5 litre) packs for laboratories, educational institutions, research facilities and other professional laboratory users.

Available Pack Sizes: 500ml | 5000ml / 5L
Product Type: Laboratory Reagent
Test Type: Qualitative reducing sugar test
Country of Origin: India
Intended Use: Laboratory use


What Is Benedict’s Reagent Used For?

Benedict’s reagent is primarily used to determine whether a suitable sample contains reducing sugars.

Reducing sugars are carbohydrates capable of participating in a reduction reaction with copper ions under the test conditions. Common reducing sugars include glucose, fructose, galactose, lactose and some other carbohydrates.

Primary Uses & Applications

  • Qualitative detection of reducing sugars
  • Carbohydrate identification experiments
  • Biochemistry laboratory practicals
  • Educational laboratory demonstrations
  • School and college chemistry or biology laboratory experiments
  • Research laboratory applications
  • Teaching laboratories
  • Routine laboratory procedures where Benedict’s qualitative reaction is specified

Important: Suitability for a particular sample, testing protocol or diagnostic procedure should always be confirmed against the laboratory SOP, product label and applicable testing method before use.


How Does Benedict’s Reagent Work?

Benedict’s reagent contains copper(II) ions supplied by copper sulphate in an alkaline environment created by sodium carbonate. Sodium citrate helps keep the copper ions in solution.

When a reducing sugar is present and the reaction mixture is heated under suitable conditions, the reducing sugar reduces copper(II) ions (Cu²⁺) to copper(I) ions (Cu⁺).

This results in the formation of insoluble copper(I) oxide (Cu₂O), which can produce a visible coloured precipitate.

Typical Colour Reaction

Depending on the test method and amount of reducing sugar present, the reaction may progress from the original blue reagent towards shades such as:

  • Green
  • Yellow
  • Orange
  • Orange-red
  • Brick red

The colour reaction should be interpreted according to the validated laboratory method being followed. Benedict’s qualitative test should not be treated as a precise quantitative measurement unless an appropriately validated quantitative procedure is being used.


How to Use Benedict’s Reagent Solution

The exact test procedure can vary according to the laboratory method, sample type and SOP being followed.

General Laboratory Procedure

  1. Use a clean and dry laboratory test tube.
  2. Add the required quantity of Benedict’s Reagent according to your laboratory SOP.
  3. Add the specified quantity of the test sample.
  4. Mix carefully.
  5. Heat the reaction mixture using the prescribed laboratory heating method, commonly a controlled hot-water or boiling-water bath where applicable.
  6. Observe any colour change or precipitate formation.
  7. Interpret the result according to the validated test method or laboratory SOP.

Do not rely on a generic internet procedure when working under an accredited, clinical, research or institutional laboratory protocol. Always follow the laboratory’s validated procedure and the instructions supplied with the reagent.


Benedict’s Reagent Composition & Principle

A conventional Benedict’s qualitative reagent is based primarily on:

  • Copper sulphate: provides copper(II) ions for the oxidation-reduction reaction.
  • Sodium carbonate: provides the alkaline environment required for the reaction.
  • Sodium citrate: complexes with copper ions and helps maintain them in solution under alkaline conditions.

The exact formulation or concentration of a commercially supplied reagent should be confirmed from the product label, manufacturer information or accompanying documentation where required.


Product Features

  • Ready-to-use liquid laboratory reagent
  • Suitable for qualitative reducing sugar testing
  • Convenient alternative to preparing Benedict’s reagent in the laboratory
  • Suitable for laboratory and educational applications
  • Available in multiple pack sizes
  • 500ml pack suitable for routine or lower-volume requirements
  • 5 litre pack suitable for laboratories with larger consumption
  • Made in India

Product Specifications

Product Name Benedict’s Reagent Solution
Also Known As Benedict’s Solution / Benedict’s Qualitative Reagent
Product Type Laboratory Reagent
Primary Application Qualitative detection of reducing sugars
Form Liquid solution
Available Pack Sizes 500ml and 5000ml / 5L
Major Reagent Components Copper sulphate, sodium citrate and sodium carbonate
Intended Users Laboratories, educational institutions, research facilities and trained laboratory personnel
Country of Origin India

Benefits of Ready-to-Use Benedict’s Solution

  • Convenient: eliminates routine preparation of the reagent from individual chemicals.
  • Time saving: suitable for laboratories performing repeated teaching or qualitative testing procedures.
  • Multiple pack sizes: select 500ml for regular requirements or 5L for higher-volume consumption.
  • Easy procurement: order online through Microsidd.com.
  • Laboratory focused: suitable for professional and educational laboratory environments.

Who Can Buy Benedict’s Reagent?

Benedict’s Reagent Solution may be useful for:

  • Diagnostic laboratories where an approved SOP specifies the reagent
  • Pathology and clinical laboratory professionals
  • Schools and colleges
  • Biochemistry laboratories
  • Chemistry laboratories
  • Research institutions
  • Training laboratories
  • Universities
  • Laboratory supply dealers
  • Institutional procurement departments

Where to Buy Benedict’s Reagent Solution in India?

You can buy Benedict’s Reagent Solution online from Microsidd India through Microsidd.com.

Customers looking for Benedict’s reagent in Bangalore or Bengaluru can order online or contact our Bengaluru location regarding product availability and eligible local purchase or pickup options.

Microsidd India
09, 2nd Floor, 14th Cross
RT Nagar, Bengaluru – 560032
Karnataka, India
Phone / WhatsApp: +91 63667 72288
Website: microsidd.com

View Microsidd India – Bengaluru Location & Local Medical/Laboratory Supply Information

You can also explore other laboratory products and reagents available from Microsidd India.


Why Buy Benedict’s Reagent from Microsidd India?

Customers buying laboratory reagents often have three choices: a specialist supplier such as Microsidd India, a general online marketplace, or an offline laboratory supply shop.

Buying Factor Microsidd India General Online Store / Marketplace Offline Store
Laboratory Product Focus Wide laboratory, diagnostic and consumable catalogue Varies by seller Depends on individual store
Pack Selection 500ml and 5L options where available May vary by listing Limited to available stock
Product Information Detailed product-specific information and laboratory-use guidance Often seller dependent May require in-store enquiry
Online Ordering Available directly from Microsidd.com Usually available May not be available
Bengaluru Local Support RT Nagar, Bengaluru location Marketplace dependent Depends on shop location
Related Laboratory Products Reagents, consumables, blood collection supplies and laboratory equipment available in one catalogue May involve multiple sellers Stock varies

Storage & Handling

  • Store according to the storage instructions printed on the supplied product label.
  • Keep the container tightly closed when not in use.
  • Protect the reagent from contamination.
  • Use clean laboratory glassware and dispensing equipment.
  • Do not interchange caps or dispensing accessories between reagent containers.
  • Observe appropriate laboratory chemical-handling procedures.
  • Refer to the applicable Safety Data Sheet where required.
  • Do not use beyond the labelled expiry date.

Always treat the storage conditions stated on the supplied label or manufacturer documentation as authoritative for the specific batch received.


Frequently Asked Questions – Benedict’s Reagent

1. What is Benedict’s Reagent Solution used for?

Benedict’s Reagent is commonly used for the qualitative detection of reducing sugars in suitable laboratory samples.

2. What does Benedict’s reagent detect?

It detects substances capable of reducing copper(II) ions under the alkaline heated conditions of the Benedict reaction, including many common reducing sugars.

3. Is Benedict’s reagent used for glucose testing?

Glucose is a reducing sugar and can react with Benedict’s reagent. However, Benedict’s qualitative reagent is not a substitute for a modern quantitative blood glucose measurement system or a validated clinical glucose analyser.

4. What colour is Benedict’s reagent before the reaction?

Conventional Benedict’s qualitative reagent is typically blue because of the copper(II) ions present in the solution.

5. What indicates a positive Benedict’s test?

Under the appropriate test conditions, development of a coloured precipitate or colour transition away from the original blue may indicate the presence of reducing substances. Interpretation must follow the laboratory method being used.

6. Does Benedict’s reagent require heating?

The conventional Benedict qualitative reaction normally requires heating under controlled laboratory conditions. Follow your validated SOP and product instructions.

7. Is Benedict’s reagent quantitative?

This listing is for Benedict’s reagent used primarily for qualitative testing. Colour intensity can vary with reducing-sugar concentration, but visual interpretation should not be considered a precise quantitative measurement unless a validated quantitative method is specifically being used.

8. What pack sizes are available?

This product is available in 500ml and 5000ml / 5 litre pack sizes.

9. Can schools and colleges buy Benedict’s reagent?

Yes. Benedict’s reagent is commonly used in educational chemistry, biology and biochemistry laboratory demonstrations, subject to the institution’s laboratory safety procedures.

10. Can laboratories buy Benedict’s reagent in bulk?

Yes. The 5 litre pack can be considered for higher-volume requirements. Institutional and bulk buyers may contact Microsidd India for current availability.

11. Where can I buy Benedict’s reagent in Bangalore?

Microsidd India supplies laboratory products from RT Nagar, Bengaluru. Customers can order through Microsidd.com or contact the Bengaluru location regarding current availability and eligible local purchase or pickup options.

12. Is Benedict’s reagent the same as Fehling’s solution?

No. Both can be used in reducing-sugar chemistry, but their formulations and preparation differ. Use the reagent specified by your laboratory method or SOP.


Important: Verify Before Ordering

Please verify the following before placing your order:

  • Product name
  • Required reagent
  • Required application
  • Laboratory method
  • Pack size
  • Quantity
  • Any required grade or specification
  • Compatibility with your laboratory SOP

Laboratory reagents with similar names may have different formulations, grades, concentrations or intended applications. Do not order solely on the basis of a generic reagent name if your laboratory protocol specifies a particular composition or specification.


No Return / No Exchange Policy

This product is non-returnable and non-exchangeable.

Please verify the product name, reagent, application, formulation where applicable, pack size, quantity and specifications before placing your order.

Genuine cases involving an incorrect product received will be handled separately according to Microsidd.com’s applicable policies and applicable law.

An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect or damaged product received.

When opening the shipment, carefully cut the sealing tape with a blade or suitable cutter; do not tear the package open, so that the packaging and product condition remain clearly visible in the continuous unboxing video.


Medical & Laboratory Disclaimer

This product is intended for laboratory use by appropriately trained personnel. Information on this page is provided for product identification and general educational purposes and should not replace the manufacturer’s instructions, laboratory SOP, validated test method, Safety Data Sheet or professional laboratory judgement.

Benedict’s Reagent Solution should not be used by consumers as a substitute for a validated medical diagnostic test, blood glucose meter, laboratory analyser or professional medical evaluation.

Any laboratory result that may influence diagnosis, treatment or clinical decision-making must be obtained and interpreted using an appropriately validated method by qualified healthcare or laboratory professionals.


Looking for laboratory reagents in Bengaluru or anywhere in India?
Explore Laboratory Needs at Microsidd India or visit our Microsidd India Bengaluru location page for local business and contact information.

Product specifications table
Specification name Specification Value
Chemical grade Laboratory
Color pattern Navy
Test method Lab analysis

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