Rapitest HIV 1 / 2 Serum (Flow - Through) Test is a membrane based test, which is coated with recombinant antigens specific to HIV 1 antibodies at test region “1” and HIV 2 antibody specific antigens at test region “2”. The control Region “C” is a performance control. The reaction well is initially washed with a wash buffer in order to make the membrane ready for testing, then the serum / plasma specimen is added. The respective antigens coated in the membrane captures respective antibodies to HIV 1 / 2 in the serum / plasma, if the specimen has antibodies to HIV 1 / 2. The membrane is again washed to remove excess and unreacted antibodies in the membrane. Prior washing, Protein A conjugated gold sol reagent is added to visualize the presence of bound antigen & antibody complex. A final wash is done to clear the back ground left with pink bands. Bands at test region “1” & “2” and control region “C” is a positive result and absence of bands at test region “1” & “2” is a negative result. No band at region “C” indicates the test is “INVALID”
