BIOLAB Gram’s Iodine is a professional microbiology staining reagent supplied in a 100 mL pack for use as the iodine mordant in validated Gram staining procedures.
Gram staining is one of the most widely used differential staining methods in microbiology. Within the conventional staining sequence, Gram’s Iodine is applied after the primary crystal-violet stain and before the decolorisation step.
The iodine acts as a mordant, interacting with the primary dye within the stained bacterial cells so that subsequent decolorisation can produce the differential staining pattern used for microscopic classification.
Professional laboratory use only. Smear preparation, fixation, staining sequence, reagent exposure time, decolorisation, counterstaining, microscopy, quality control and interpretation must follow the current BIOLAB instructions and the laboratory's validated SOP.
| Product Name | Gram’s Iodine |
| Brand | BIOLAB Diagnostics |
| Pack Size | 100 mL |
| Product Category | Microbiology Staining Reagent |
| Primary Application | Gram Staining |
| Role in Gram Stain | Iodine Mordant |
| Used After | Primary Crystal Violet Staining |
| Used Before | Decolourisation Step |
| Specimen Format | Prepared Microbial / Bacterial Smear according to validated laboratory protocol |
| Use Environment | Professional Laboratory Use |
Gram’s Iodine is the mordant used in the traditional Gram-staining sequence.
The Gram-staining method uses a sequence of reagents to create a differential microscopic staining pattern between organisms that retain the primary dye complex after decolorisation and those that do not.
Gram’s Iodine is therefore not normally used as a standalone stain. It performs a specific intermediate function within the complete Gram-staining procedure.
After the bacterial smear has been treated with the primary crystal-violet stain, Gram’s Iodine is applied as the mordant.
The iodine interacts with the primary stain within the bacterial cells and helps form a dye–iodine complex.
The subsequent decolorisation stage then differentiates organisms according to how the stained cell structure responds to the validated Gram-staining procedure.
The final microscopic appearance is also influenced by:
| Step | Reagent / Process | General Function |
|---|---|---|
| 1 | Primary Stain | Crystal violet staining |
| 2 | Gram’s Iodine | Mordant step |
| 3 | Decolouriser | Differential decolourisation |
| 4 | Counterstain | Provides contrasting staining of appropriately decolourised organisms |
Important: The exact staining time and handling procedure should be taken from the current BIOLAB instructions or your laboratory's validated SOP rather than from a generic online protocol.
Gram staining is a differential microbiological staining technique.
When the complete procedure is performed correctly, organisms are differentiated according to their staining response after the primary stain, iodine mordant, decolourisation and counterstain sequence.
In conventional Gram staining:
Microscopic colour alone should not be used as the sole basis for organism identification or clinical diagnosis.
BIOLAB Gram’s Iodine is relevant to professional laboratory environments including:
Correct Gram staining begins with a properly prepared smear on a clean microscope slide.
Microsidd supplies Microscope Glass Slides for professional laboratory microscopy and specimen preparation.
You can also browse the Microscope Slides & Laboratory Glassware Collection for slides, cover slips and related laboratory products.
BIOLAB Gram’s Iodine forms part of Microsidd India's growing professional staining-reagent and laboratory chemical catalogue.
Browse:
This internal product structure enables laboratories to source staining reagents, slides and other professional laboratory supplies through the same specialist catalogue.
Store BIOLAB Gram’s Iodine according to the exact conditions printed on the current product label and manufacturer documentation.
Good laboratory practice includes:
If the current BIOLAB bottle label, package insert or Safety Data Sheet differs from general information displayed online, follow the current manufacturer documentation supplied with the reagent.
Microbiology staining reagents should be selected by verifying the exact stain, reagent stage, brand, pack size and laboratory application.
Microsidd India combines online ordering with a Bengaluru-based diagnostic and laboratory-products business and provides detailed product information intended to help professional buyers verify the correct reagent before ordering.
| Buying Consideration | Microsidd India | General Local Market / Marketplace |
|---|---|---|
| Exact Reagent Identification | BIOLAB Gram’s Iodine 100 mL clearly identified | Information varies by seller |
| Gram-Stain Stage | Clearly identified as the iodine mordant step | May be shown only as a generic stain |
| Pack-Size Clarity | 100 mL clearly stated | May vary between listings or variants |
| Laboratory Product Range | Related stains, reagents, slides and laboratory supplies available | Depends on individual seller |
| GST Invoice | Available for eligible purchases | Depends on seller |
| Bengaluru Presence | Physical business presence in RT Nagar, Bengaluru | Depends on seller/location |
| Institutional Procurement | Laboratory and bulk requirements may be supported subject to availability | Varies by seller |
| India-Wide Ordering | Delivery to serviceable locations across India | Depends on platform and seller |
Microsidd aims to provide enough information for professional laboratory buyers to verify the exact reagent before purchase rather than relying only on product images or abbreviated marketplace titles.
Microsidd India is a medical, diagnostic and laboratory-products supplier based in RT Nagar, Bengaluru.
Laboratories searching for Gram’s Iodine in Bangalore, BIOLAB Gram’s Iodine Bengaluru, Gram stain reagent supplier Bangalore, microbiology stains Bengaluru, Gram staining reagent near RT Nagar or professional laboratory reagents in Bangalore can check current availability through Microsidd.com.
For a specific reagent or planned local pickup, checking stock before visiting is recommended because inventory availability can change.
Business Name: Microsidd India
Address: #09, Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India
Phone / WhatsApp: +91 63667 72288
Email: support@microsidd.com
Website: https://microsidd.com
Working Hours: Monday to Saturday, 10:00 AM – 6:00 PM IST
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For Bengaluru store and business details, visit Microsidd India – Medical & Laboratory Supplies Bangalore.
BIOLAB Gram’s Iodine is a professional microbiology reagent supplied for use in Gram-staining procedures.
This Microsidd listing is for 100 mL.
Gram’s Iodine is used as the mordant after the primary crystal-violet staining stage in a conventional Gram-staining sequence.
No. The primary stain in conventional Gram staining is crystal violet. Gram’s Iodine is the subsequent mordant reagent.
A mordant is a reagent used to help stabilise or strengthen the interaction between a stain and the material being stained. In Gram staining, iodine interacts with the primary crystal-violet stain before the decolourisation step.
It is generally applied after crystal violet and before the decolourising reagent. Follow the exact BIOLAB instructions or validated laboratory SOP for timing and washing.
In the conventional Gram-staining sequence, an appropriate decolourising reagent follows the iodine mordant stage, followed by a counterstain.
No. Differential classification requires the complete validated Gram-staining sequence and professional microscopic interpretation.
In a correctly performed conventional Gram stain, organisms retaining the primary dye–iodine complex typically appear purple to blue.
After appropriate decolourisation and counterstaining, organisms that do not retain the primary dye complex typically appear pink to red.
Gram-stain morphology provides useful preliminary laboratory information but does not by itself definitively identify every bacterial species. Additional culture, biochemical, molecular or other laboratory testing may be required.
Not for the standard Gram-staining procedure. Gram’s Iodine is intended to function after the primary stain within the validated multi-step workflow.
No. This listing is for BIOLAB Gram’s Iodine 100 mL only. Other Gram-staining reagents should be purchased separately unless explicitly stated otherwise.
No. The 100 mL Gram’s Iodine bottle is a standalone reagent listing unless the product title or pack contents specifically state otherwise.
No. Microscope slides are separate laboratory consumables and are available independently from Microsidd.
Do not apply a generic online timing automatically to this product. Follow the current BIOLAB instructions and the laboratory's validated SOP.
Follow the exact storage conditions printed on the current BIOLAB bottle label and accompanying manufacturer documentation. Keep the bottle tightly closed and protected from contamination.
No. Professional laboratory reagents should not be used beyond the manufacturer's labelled expiry date.
No. BIOLAB Gram’s Iodine is intended for professional laboratory use by appropriately trained personnel.
BIOLAB Gram’s Iodine 100 mL can be ordered through Microsidd.com subject to stock availability. Microsidd India is located in RT Nagar, Bengaluru.
Microsidd supplies laboratory products to serviceable locations across India subject to product availability, handling requirements and courier serviceability.
Bulk and institutional requirements may be supported subject to current inventory, quantity and applicable commercial terms.
BIOLAB Gram’s Iodine is intended for professional laboratory use by appropriately trained personnel.
The information on this page is provided for product identification, procurement and general educational purposes and does not replace:
Gram staining provides preliminary morphological and staining information and does not by itself establish a complete microbiological or clinical diagnosis.
Interpretation should be performed by appropriately trained personnel and correlated with culture, biochemical, molecular or other investigations where clinically or laboratorily appropriate.
If the current BIOLAB bottle label, package insert or Safety Data Sheet differs from general information displayed online, follow the current manufacturer documentation supplied with the reagent.
This laboratory reagent is non-returnable and non-exchangeable once correctly supplied.
Please verify before ordering:
Ordering the wrong reagent, staining component or pack size is not considered a product defect when the supplied product matches the confirmed order.
Laboratory reagents may be affected by contamination, storage conditions, temperature exposure and handling after delivery. Correctly supplied reagents therefore cannot normally be accepted back into saleable inventory.
Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or other remedies required under applicable law will be handled according to the Microsidd Refund, Return, Replacement & Cancellation Policy.
An original, continuous, unedited and uncut unboxing video is compulsory for delivery-related claims involving an incorrect, missing or damaged product.
The recording must begin before the parcel is opened. Open the carton carefully by cutting the sealing tape with a blade or appropriate cutter; do not tear open the carton. Keep the courier label, sealed parcel, product and condition continuously visible during opening.
Product: Gram’s Iodine
Brand: BIOLAB Diagnostics
Pack Size: 100 mL
Category: Microbiology Staining Reagent
Primary Application: Gram Staining
Role: Mordant after primary crystal-violet stain
Use: Professional Laboratory Use Only
Typical Users: Microbiology, Diagnostic, Pathology, Hospital, Teaching & Research Laboratories
Supplier: Microsidd India
Supplier Location: RT Nagar, Bengaluru, Karnataka, India
| Specification name | Specification Value |
|---|---|
| Chemical grade | Laboratory |
| Color pattern | Brown |
