# Uric Acid Reagent Kit – 25 ml, 50 ml & 100 ml | Uricase / PAP Method

# Swemed Uric Acid Reagent Kit

The **Swemed Uric Acid Reagent Kit** is a professional clinical biochemistry reagent intended for the **quantitative determination of uric acid in serum, plasma and fresh urine** using the **Uricase / PAP enzymatic method**.

Swemed's technical literature describes an enzymatic reaction in which **uricase converts uric acid to allantoin and hydrogen peroxide**. The hydrogen peroxide subsequently reacts with a phenolic compound and 4-aminoantipyrine in the presence of peroxidase to form a red quinoneimine dye. The intensity of the resulting colour is directly proportional to the amount of uric acid present in the specimen.

For the Microsidd Shopify listing, this product is offered in convenient **25 ml, 50 ml and 100 ml pack-size options**.

Swemed's current catalogue lists **Uric Acid 25 ml and 2 × 50 ml** under product code **UA 724**, while the technical insert also documents **2 × 25 ml and 2 × 50 ml** configurations. This supports laboratory procurement across 25 ml, 50 ml and 100 ml total reagent quantities depending on the supplied configuration.

[**Also available as part of the Swemed RFT Reagent Combo Kit**](https://microsidd.com/products/rft-reagent-combo-kit-urea-uv-creatinine-uric-acid "Also available as part of the Swemed RFT Reagent Combo Kit")

* * *

# Available Pack Sizes

Variant

Reagent Configuration

**25 ml**

Compact 25 ml presentation

**50 ml**

**2 × 25 ml** configuration

**100 ml**

**2 × 50 ml** configuration

Swemed's technical insert identifies **UA50** for 2 × 25 ml and **UA100** for 2 × 50 ml, while its current catalogue also lists a 25 ml presentation under product code UA 724.

# Product at a Glance

Product Detail

Information

Product Name

Uric Acid Reagent Kit

Brand

Swemed Diagnostics

Available Pack Sizes

**25 ml / 50 ml / 100 ml**

Test Parameter

Uric Acid

Method

**Uricase / PAP**

Assay Type

Enzymatic Colorimetric

Mode

**Endpoint**

Wavelength

**505 nm**

Temperature

**37°C**

Standard Concentration

**5 mg/dL**

Reaction Direction

Increasing

Reagent Volume

**1000 µl**

Sample Volume

**50 µl**

Incubation

**10 minutes at 37°C**

Measuring Range

**1–25 mg/dL**

Linearity

**Up to 25 mg/dL**

Specimens

Serum / Heparin Plasma / EDTA Plasma / Fresh Urine

Reagent Preparation

Ready to Use

Storage

**2–8°C**

Freezing

Do Not Freeze

Intended Use

Professional In Vitro Diagnostic Use

Seller

Microsidd India

Website

microsidd.com

Location

Bangalore / Bengaluru, Karnataka, India

The analytical parameters above are specified in the Swemed Uric Acid technical insert.

# Key Features

-   **Swemed Diagnostics Uric Acid Reagent Kit**
-   Available in **25 ml, 50 ml and 100 ml**
-   Established **Uricase / PAP enzymatic method**
-   Ready-to-use reagent
-   Ready-to-use uric acid standard
-   Endpoint colorimetric assay
-   Recommended wavelength: **505 nm**
-   Standard concentration: **5 mg/dL**
-   Reagent volume: **1000 µl**
-   Sample volume: **50 µl**
-   Incubation: **10 minutes at 37°C**
-   Measuring range: **1–25 mg/dL**
-   Linearity up to **25 mg/dL**
-   Suitable for serum and compatible plasma
-   Suitable for fresh urine
-   Suitable for compatible open-system semi-auto analysers
-   Suitable for compatible open-system fully automatic analysers
-   For **professional in vitro diagnostic use only**

# Key Benefits

## Enzymatic Uricase Method

The assay uses **uricase and peroxidase-based chemistry**, providing an established enzymatic method for quantitative uric acid measurement.

## Ready-to-Use Reagent

Swemed states that both the **reagent and standard are ready to use**, simplifying routine laboratory workflow.

## Multiple Pack Choices

The availability of **25 ml, 50 ml and 100 ml total reagent options** allows laboratories to choose according to testing volume, reagent turnover and workload.

## Useful for Renal and Metabolic Profiles

Uric acid is frequently included alongside:

-   Urea
-   Creatinine
-   Sodium
-   Potassium
-   Chloride
-   Calcium
-   Other renal and metabolic parameters

## Open-System Compatibility

The assay parameters can be programmed on compatible **open-system semi-automatic and fully automatic biochemistry analysers**, subject to validation by the laboratory.

# Intended Use

The **Swemed Uric Acid Reagent Kit** is intended for quantitative measurement of **uric acid in professional clinical chemistry laboratories**.

The manufacturer technical insert lists:

-   Serum
-   Heparin plasma
-   EDTA plasma
-   Fresh urine

Swemed's online biochemistry catalogue also identifies Uric Acid as a **Uricase/PAP method** assay for serum and urine.

This product is for **professional in vitro diagnostic use only**.

# Test Principle

The assay proceeds through two main enzymatic reactions.

First:

**Uric Acid + H₂O —Uricase→ Allantoin + H₂O₂**

The hydrogen peroxide formed then reacts with **4-aminoantipyrine and a phenolic compound in the presence of peroxidase** to produce a red quinoneimine dye.

The intensity of the colour formed is proportional to the amount of uric acid present in the sample.

# Reagent Composition

According to Swemed's product literature:

Component

Manufacturer Specification

Uricase

**200 U/L**

Peroxidase

**4 kU/L**

BSA

**1 g/L**

Triton X-100

**4 ml/L**

Uric Acid Standard

**5 mg/dL**

# Reagent Preparation

**The reagent and standard are ready to use.**

No routine reagent reconstitution is required before following the manufacturer assay procedure.

# Technical Specifications

Parameter

Swemed Specification

Test

Uric Acid

Method

Uricase / PAP

Mode

**Endpoint**

Wavelength

**505 nm**

Unit

**mg/dL**

Temperature

**37°C**

Standard

**5 mg/dL**

Reaction Slope

Increasing

Reagent Volume

**1000 µl**

Sample Volume

**50 µl**

Incubation

**10 minutes at 37°C**

Blanking

Reagent Blank

Measuring Range

**1–25 mg/dL**

Linearity

**25 mg/dL**

# Assay Procedure Summary

Tube

Reagent

Standard / Sample

Blank

1000 µl

—

Standard

1000 µl

50 µl Standard

Test

1000 µl

50 µl Sample

Mix and incubate for:

**10 minutes at 37°C**

Then read the absorbance against the reagent blank at:

**505 nm**.

# Calculation

**Uric Acid (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration**

Standard concentration:

**5 mg/dL**.

# Compatible with Open-System Biochemistry Analysers

The **Swemed Uric Acid Reagent Kit** can be used with compatible programmable open-system analysers where the required assay parameters can be entered and validated.

Open-System Setting

Uric Acid Application

Method

Uricase / PAP

Mode

Endpoint

Wavelength

**505 nm**

Temperature

**37°C**

Reagent Volume

**1000 µl**

Sample Volume

**50 µl**

Standard

**5 mg/dL**

Incubation

**10 minutes**

Reaction

Increasing

Blank

Reagent Blank

Unit

mg/dL

Suitable system categories include:

-   **Open-system semi-automatic biochemistry analysers**
-   **Open-system fully automatic biochemistry analysers**
-   Programmable clinical chemistry systems supporting 505 nm endpoint measurement

The laboratory should verify analyser compatibility and validate the reagent application before routine patient reporting.

# Specimen Requirements

Swemed lists the following suitable specimens:

-   **Serum**
-   **Heparin plasma**
-   **EDTA plasma**
-   **Fresh urine**

The manufacturer recommends using **fresh, clear, non-haemolysed serum from fasting patients** where appropriate.

Bacterial contamination should be avoided because it may affect uric acid concentration during storage.

# Specimen Stability

According to the Swemed technical insert:

Storage Condition

Stability

20–25°C

**2 days**

2–8°C

**3–5 days**

−20°C

**6 months**

Contaminated specimens should be discarded.

# Measuring Range and Linearity

The assay is designed for uric acid concentrations between:

**1–25 mg/dL**

The stated linearity is:

**25 mg/dL**.

If a result exceeds the measuring range, Swemed instructs laboratories to dilute the sample **1:1 with 0.9% NaCl solution**, repeat the assay and multiply the obtained result by **2**.

# Interfering Substances

Swemed identifies several important analytical considerations.

### Ascorbic Acid

Elevated ascorbic-acid concentrations may produce **falsely depressed uric acid values**.

### Lipemia

Lipemic samples may cause **falsely elevated results**.

### Haemoglobin

Haemoglobin up to approximately **100 mg/dL** was reported to have negligible influence within the manufacturer's stated tolerance, while higher concentrations may produce falsely elevated uric acid values.

### Bilirubin

Bilirubin up to **30 mg/dL** was reported to have negligible interference under the evaluated assay conditions.

# Manufacturer Reference Range

Group

Swemed Reference Range

Men

**3.4–7.0 mg/dL**

Women

**2.5–6.0 mg/dL**

Swemed recommends that each laboratory establish or verify its own reference interval appropriate to its patient population.

# Storage and Stability

Store the reagent:

-   At **2–8°C**
-   Protected from light
-   Protected from contamination
-   **Do not freeze**
-   Use within the labelled expiry period
-   Bring reagents and specimens to room temperature as required before testing

The current Swemed catalogue lists **24-month stability** for its Uric Acid reagent when stored correctly.

# Signs of Reagent Deterioration

The manufacturer advises against using the reagent if:

-   It becomes visibly turbid
-   Obvious microbial growth is present
-   The reagent blank absorbance reaches **0.250 or higher at 505 nm**

# Safety and Precautions

Swemed states that the reagent contains **sodium azide at 0.95 g/L as preservative**.

Professional laboratory users should:

-   Wear suitable gloves
-   Use appropriate eye / face protection
-   Avoid skin and mucous-membrane contact
-   Never pipette by mouth
-   Follow Good Laboratory Practice
-   Handle reagent waste according to institutional procedures

# Why Uric Acid Testing Is Important

Uric acid is the final product of **purine metabolism** and is eliminated mainly through the kidneys.

Swemed notes that increased uric acid concentrations may occur in conditions including gout and impaired renal function, while reduced levels may occur in selected metabolic or hepatic disorders.

Uric acid testing is commonly used as part of:

-   Renal-function assessment
-   Hyperuricaemia investigation
-   Gout assessment
-   Metabolic profiles
-   Routine health screening
-   Monitoring of selected therapies
-   Kidney-function profiles
-   Clinical chemistry investigations

Laboratory results should always be interpreted in the appropriate clinical context.

# Uric Acid in an RFT / Renal Profile

Parameter

Laboratory Relevance

Uric Acid

Purine metabolism and renal excretion

Urea

Protein metabolism and renal excretion

Creatinine

Renal filtration-related biochemical marker

Sodium

Fluid and electrolyte balance

Potassium

Intracellular / renal electrolyte balance

Chloride

Fluid and acid-base balance

Calcium

Mineral metabolism

Magnesium

Mineral and metabolic balance

This product is therefore particularly suitable for linking internally to your upcoming **Swemed RFT Reagent Combo Kit**.

# Who Can Buy This Product?

Buyer Type

Typical Application

Pathology Laboratories

Routine uric acid estimation

Diagnostic Centres

Renal and metabolic profiles

Hospital Laboratories

Clinical chemistry

Nephrology Centres

Renal-profile investigations

Rheumatology Centres

Uric acid-related investigations

Gout Clinics

Hyperuricaemia assessment

Diabetes Centres

Metabolic and renal profiles

Clinical Chemistry Laboratories

Routine enzymatic testing

Medical Institutions

Teaching and diagnostic laboratory use

Laboratory Distributors

Professional procurement

# Useful Tips Before Ordering

-   Confirm that **Uric Acid – Uricase / PAP Method** is required.
-   Select the appropriate pack size: **25 ml, 50 ml or 100 ml**.
-   Confirm that your analyser supports **505 nm endpoint measurement**.
-   Verify that the analyser is an **open system** before programming third-party reagents.
-   Confirm sample volume: **50 µl**.
-   Confirm reagent volume: **1000 µl**.
-   Verify standard concentration: **5 mg/dL**.
-   Avoid contaminated specimens.
-   Evaluate haemolysis and lipemia before testing.
-   Store reagent at **2–8°C**.
-   Do not freeze.
-   Perform suitable calibration / standardisation and internal QC before routine use.

# Buy Swemed Uric Acid Reagent in Bangalore

Microsidd India supplies **renal-function reagents, biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka**.

The **Swemed Uric Acid Reagent Kit** is available in **25 ml, 50 ml and 100 ml total reagent options**, making it suitable for laboratories with different testing volumes.

It can be ordered through **microsidd.com**, subject to stock availability and delivery serviceability.

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# Product Summary

### What is Swemed Uric Acid Reagent Kit?

The **Swemed Uric Acid Reagent Kit** is a professional clinical biochemistry reagent for quantitative uric acid determination using the **Uricase / PAP enzymatic endpoint method**.

### Which pack sizes are available?

Microsidd lists:

-   **25 ml**
-   **50 ml**
-   **100 ml**

The manufacturer literature documents a 25 ml presentation as well as 2 × 25 ml and 2 × 50 ml configurations across its current catalogue and technical insert.

### What method is used?

**Uricase / PAP Method.**

### What wavelength is required?

**505 nm.**

### What is the sample volume?

**50 µl.**

### What is the reagent volume?

**1000 µl.**

### What is the standard concentration?

**5 mg/dL.**

### What is the incubation time?

**10 minutes at 37°C.**

### What is the measuring range?

**1–25 mg/dL.**

### What is the linearity?

**25 mg/dL.**

### Is the reagent ready to use?

Yes. Swemed states that both reagent and standard are **ready to use**.

### Which specimens can be tested?

Serum, heparin plasma, EDTA plasma and fresh urine are listed in the manufacturer insert.

### Is it compatible with semi-automatic analysers?

Yes, on compatible **open-system semi-automatic biochemistry analysers** after method programming and laboratory validation.

### Is it compatible with fully automatic analysers?

Yes, on compatible **open-system fully automatic biochemistry analysers** that permit third-party reagent programming and support the required endpoint application.

# Entity Information

Entity

Details

Product

Uric Acid Reagent Kit

Brand

Swemed Diagnostics

Pack Sizes

25 ml / 50 ml / 100 ml

Manufacturer Product Code

UA 724

Analyte

Uric Acid

Method

Uricase / PAP

Mode

Endpoint

Wavelength

505 nm

Standard

5 mg/dL

Measuring Range

1–25 mg/dL

Clinical Category

Renal / Metabolic Profile

Product Category

Laboratory Reagents

Subcategory

Biochemistry Reagents

Analyser Compatibility

Open-System Fully Automatic & Semi-Automatic Analysers

Seller

Microsidd India

City

Bangalore / Bengaluru

State

Karnataka

Country

India

Website

microsidd.com

  

# Why Buy Swemed Uric Acid Reagent from Microsidd.com?

Clinical chemistry reagents should be purchased only after verifying the **correct parameter, methodology, pack configuration, wavelength, sample requirements, storage conditions and analyser compatibility**.

Microsidd India specialises in laboratory and diagnostic products and has long-standing experience in the IVD sector, allowing professional laboratory buyers to purchase from an identifiable diagnostic-product supplier rather than relying only on generic marketplace listings.

## Microsidd.com vs Online Marketplaces vs Offline Suppliers

Buying Factor

Microsidd.com

General Online Marketplaces

Offline Suppliers

Diagnostic Specialisation

**Laboratory / IVD focused**

Seller expertise varies

Dealer dependent

Pack Options

**25 ml / 50 ml / 100 ml clearly organised**

Listings may vary

Stock dependent

Method Verification

Uricase / PAP clearly identified

Seller dependent

Dealer dependent

Analyser Guidance

Better suited to open-system queries

Often limited

Depends on technical knowledge

Seller Identity

Microsidd India

Marketplace seller may vary

Usually identifiable

GST Invoice

Available for eligible orders

Seller dependent

May vary

RFT Integration

Links naturally with Urea & Creatinine

Usually separate listings

Dealer dependent

Product Information

Technical specifications available

Listing dependent

Often verbal

B2B Procurement

Suitable for laboratories and institutions

Seller dependent

Usually suitable

India-Wide Supply

Subject to serviceability

Broad

Often regional

# Benefits of Buying from Microsidd.com

Microsidd Advantage

Benefit to Laboratory Buyers

Diagnostic Product Specialisation

Better understanding of laboratory procurement

Multiple Pack Sizes

Choose according to laboratory workload

Technical Product Information

Method and assay settings available before ordering

Direct Seller Accountability

Purchase directly from Microsidd India

RFT Product Range

Urea, Creatinine and Uric Acid can be sourced together

Open-System Focus

Suitable for fully and semi-auto analyser users

GST Invoice

Useful for institutional purchasing

Bangalore-Based Operations

Convenient for Bengaluru / Karnataka laboratories

India-Wide Supply

Delivery to serviceable locations

Direct Product Support

Easier handling of product and order queries

# Frequently Asked Questions

### What is Swemed Uric Acid Reagent used for?

It is used for quantitative measurement of **uric acid in serum, plasma and fresh urine** using the enzymatic Uricase method.

### What pack sizes are available?

Microsidd lists **25 ml, 50 ml and 100 ml** variants.

### What method is used?

**Uricase / PAP.**

### What wavelength is required?

**505 nm.**

### What is the assay mode?

**Endpoint.**

### What is the reagent volume?

**1000 µl.**

### What is the sample volume?

**50 µl.**

### What is the standard concentration?

**5 mg/dL.**

### How long is the incubation?

**10 minutes at 37°C.**

### What is the linearity?

**25 mg/dL.**

### Is the reagent ready to use?

Yes. The reagent and standard are ready to use.

### Can fresh urine be used?

Yes. Fresh urine is listed as a suitable specimen in the Swemed product insert.

### How should the reagent be stored?

At **2–8°C**, protected from light and contamination. Do not freeze.

### Can the reagent be used on a semi-auto analyser?

Yes, on compatible **open-system semi-automatic biochemistry analysers** after programming and validation.

### Can it be used on a fully automatic analyser?

Yes, on compatible **open-system fully automatic biochemistry analysers** supporting the assay requirements and third-party reagent programming.

### Is this product useful for an RFT panel?

Yes. Uric Acid is commonly used alongside **Urea and Creatinine** in renal and metabolic testing.

### Is this suitable for home testing?

No. This reagent is intended for **professional in vitro diagnostic laboratory use only**.

# Medical and Laboratory Disclaimer

The information on this page is provided for **product identification, professional laboratory procurement and educational purposes only**.

This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**.

Uric acid results should not be interpreted independently to diagnose or exclude gout, kidney disease, metabolic disorders or other medical conditions. Results should be evaluated together with clinical history, Urea, Creatinine, renal-function parameters and other relevant investigations.

Specimen quality, lipemia, haemolysis, bilirubin and ascorbic acid may influence assay results under certain conditions.

For open-system fully automatic and semi-automatic analysers, **instrument compatibility, assay programming, standardisation, calibration or standard use, quality control and analytical performance must be verified and validated by the laboratory before routine patient reporting**.

The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification.

# No Return / No Exchange Policy

**This product is non-returnable and non-exchangeable.**

Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**.

For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering.

Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law.

**An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.**

## Details

- **Price:** 0.0 INR
- **Vendor:** Swemed Diagnostics
- **Type:** Biochemistry Reagents
- **Tags:** Bio Chemistry Reagents, biochemistry

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| 25ml | 0.00 INR | In stock |
| 50ml | 0.00 INR | In stock |
| 100ml | 0.00 INR | In stock |

## Images

- Uric Acid Reagent Kit – 25 ml, 50 ml & 100 ml | Uricase / PAP Method
- Swemed Uric Acid Reagent | 25ml 50ml 100ml | Uricase | Microsidd
- Swemed Uric Acid Reagent | 25ml 50ml 100ml | Uricase | Microsidd
- Swemed Uric Acid Reagent | 25ml 50ml 100ml | Uricase | Microsidd
- Swemed Uric Acid Reagent | 25ml 50ml 100ml | Uricase | Microsidd

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> Source: [Microsidd India](https://microsidd.com/products/uric-acid-reagent-kit)
> Updated: 2026-09-13
