# Urea UV Reagent Kit – 25 ml, 50 ml & 100 ml | GLDH Fixed-Time Method # Swemed Urea UV Reagent Kit The **Swemed Urea UV Reagent Kit** is a professional clinical biochemistry reagent intended for the **photometric quantitative determination of urea in serum, plasma and urine** using the **GLDH enzymatic method**. The assay is performed as a **fixed-time UV method at 340 nm**, where urea is first hydrolysed by urease to ammonia, and the ammonia then reacts with α-ketoglutarate in a reaction catalysed by **glutamate dehydrogenase (GLDH)**. During this reaction, NADH is oxidised to NAD+, producing a decrease in absorbance proportional to the amount of urea present in the sample. For your Microsidd Shopify listing, the product is available in **25 ml, 50 ml and 100 ml pack-size variants**. The reagent is suitable for compatible **open-system fully automatic and semi-automatic biochemistry analysers** that permit programming of fixed-time applications and the required wavelength, volumes and timing parameters. * * * ## Available Pack Sizes Variant Pack Size Swemed Urea UV **25 ml** Swemed Urea UV **50 ml** Swemed Urea UV **100 ml** * * * # Product at a Glance Product Detail Information Product Name Urea UV Reagent Kit Brand Swemed Diagnostics Available Pack Sizes **25 ml, 50 ml, 100 ml** Test Parameter Urea Method **GLDH Method** Assay Type UV Enzymatic Mode **Fixed Time** Wavelength **340 nm** Temperature **37°C** Standard Concentration **50 mg/dL** Reaction Direction Decreasing Reagent Volume **1000 µl** Sample Volume **10 µl** Delay Time **30 seconds** Reaction Time **60 seconds** Linearity **Up to 300 mg/dL** Specimen Serum / Plasma / Fresh Urine Working Reagent Ratio **4 parts R1 + 1 part R2** Storage **2–8°C** Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India The assay settings above are taken from the current Swemed Urea UV technical insert. * * * # Key Features - **Swemed Diagnostics Urea UV Reagent** - Available in **25 ml, 50 ml and 100 ml variants** - **GLDH enzymatic method** - Fixed-time UV assay - Measurement at **340 nm** - Standard concentration: **50 mg/dL** - Sample volume: **10 µl** - Reagent volume: **1000 µl** - Delay time: **30 seconds** - Reaction time: **60 seconds** - Linearity up to **300 mg/dL** - Working reagent prepared at **4 parts R1 + 1 part R2** - Suitable for serum and compatible plasma - Suitable for fresh urine after dilution - Compatible with open-system semi-automatic analysers - Compatible with open-system fully automatic analysers - For **professional in vitro diagnostic use only** * * * # Key Benefits ## GLDH UV Method The GLDH method provides a direct enzymatic UV approach to urea measurement and is widely used in modern clinical chemistry systems. ## Fixed-Time Measurement The Swemed Urea UV assay uses a defined **30-second delay and 60-second reaction interval**, supporting straightforward programming on compatible open-system analysers. ## Convenient Pack Choices With **25 ml, 50 ml and 100 ml variants**, laboratories can choose the pack size that best matches their daily workload and reagent turnover. ## Open-System Flexibility The reagent can be programmed on compatible **open-system fully automatic and semi-automatic analysers**, subject to validation by the laboratory. ## Useful for Renal Profiles Urea UV is commonly used together with: - Creatinine - Uric Acid - Sodium - Potassium - Chloride - Calcium - Phosphorus - Other renal chemistry parameters * * * # Compatible with Open-System Biochemistry Analysers The **Swemed Urea UV Reagent** is suitable for compatible open-system analysers that allow third-party reagent programming. Analyser Parameter Swemed Urea UV Setting Mode **Fixed Time** Wavelength **340 nm** Temperature **37°C** Standard Concentration **50 mg/dL** Reaction Direction Decreasing Reagent Volume **1000 µl** Sample Volume **10 µl** Delay Time **30 sec** Reaction Time **60 sec** Blank Water Blank Linearity **300 mg/dL** Unit mg/dL These are the quick-reference parameters provided in the current Swemed insert. ### Suitable Analyser Types - Open-system semi-automatic biochemistry analysers - Open-system fully automatic chemistry analysers - Programmable chemistry platforms supporting 340 nm UV fixed-time measurement **Compatibility and application performance should be validated by the laboratory before routine patient testing.** * * * # Intended Use The **Swemed Urea UV Reagent Kit** is intended for the quantitative photometric determination of **urea in serum, plasma or urine**. Swemed lists suitable specimen types including: - Serum - Heparin plasma, excluding ammonium heparin - Fresh urine The product is intended exclusively for **professional in vitro diagnostic use**. * * * # Test Principle Urea is enzymatically hydrolysed by urease: **Urea + H₂O → Ammonium ions + CO₂** The resulting ammonium ions react with **α-ketoglutarate and NADH** in the presence of GLDH. During this reaction, **NADH is oxidised to NAD+**, leading to a decrease in absorbance at **340 nm**. The magnitude of the absorbance change is proportional to the concentration of urea in the sample. * * * # Reagent Composition Component Manufacturer Specification R1 – Tris Buffer 9 g/L R1 – Urease 30 kU/L R1 – GLDH 4 kU/L R1 – α-Ketoglutarate 3.4 g/L R2 – NADH 1.1 g/L Standard **Urea UV 50 mg/dL** * * * # Reagent Preparation Prepare the working reagent by mixing: **4 parts R1 + 1 part R2** After mixing, Swemed instructs laboratories to leave the working reagent for at least **30 minutes at 15–25°C before use**. * * * # Working Reagent Stability Storage Condition Stability 15–25°C **5 days** 2–8°C **4 weeks** Protect the prepared working reagent from light. * * * # Technical Specifications Test Parameter Manufacturer Specification Parameter Urea UV Method GLDH Mode Fixed Time Wavelength **340 nm** Temperature **37°C** Unit mg/dL Standard **50 mg/dL** Reaction Slope Decreasing Reagent Volume **1000 µl** Sample Volume **10 µl** Delay Time **30 sec** Reaction Time **60 sec** Blanking Water Blank Linearity **300 mg/dL** * * * # Manual Assay Procedure Component Macro Method Semi-Micro Method R1 + R2 800 µl + 200 µl 400 µl + 100 µl Standard / Sample 10 µl 5 µl Mix and incubate for approximately **30 seconds at 37°C**, then record the first absorbance reading. After exactly another **60 seconds**, record the second absorbance reading. * * * # Calculation **Urea (mg/dL) = ΔA Sample ÷ ΔA Standard × Concentration of Standard** Manufacturer standard: **50 mg/dL** ### Conversion Factors - **Urea (mg/dL) × 0.1665 = Urea (mmol/L)** - **Urea (mg/dL) × 0.467 = BUN (mg/dL)** - **BUN (mg/dL) × 2.14 = Urea (mg/dL)** * * * # Specimen Requirements Swemed recommends: - Fresh, clear, non-haemolysed serum - Suitable heparin plasma - Fresh urine Important precautions include: - **Do not use ammonium heparin** - Avoid contamination with ammonia or ammonium salts - The current insert also advises against collection with fluoride where it may interfere with the assay conditions. * * * # Urine Testing Fresh urine can be tested after dilution. Swemed instructs laboratories to: **Dilute urine 1 + 49 with distilled water** Then: **Multiply the final result by 50**. * * * # Specimen Stability ## Serum / Plasma Storage Condition Stability 20–25°C **2 days** 4–8°C **7 days** −20°C **1 month** ## Urine Storage Condition Stability 20–25°C **2 days** 2–8°C **5 days** −20°C **1 month** * * * # Measuring Range and Linearity The Swemed Urea UV assay is stated to measure approximately: **2.0–300 mg/dL** with linearity up to: **300 mg/dL**. If the result exceeds the linearity limit, the manufacturer recommends dilution **1:2 with 0.9% NaCl solution** and multiplication of the obtained result by **3**. * * * # Interference Information Swemed reports no significant interference under the evaluated conditions from: Interferent Tested Level Bilirubin Up to **40 mg/dL** Haemoglobin Up to **500 mg/dL** Ascorbate Up to **30 mg/dL** Triglycerides Up to **2000 mg/dL** * * * # Manufacturer Reference Range Specimen Manufacturer Reference Serum / Plasma **14–40 mg/dL** Urine Up to **20 g/L** Each laboratory should verify its own population-specific reference interval. * * * # Storage and Stability Store the reagents: - At **2–8°C** - Protected from light - Protected from contamination - **Do not freeze** - Bring reagents and specimens to room temperature before use when required by the procedure Swemed states that reagent deterioration may be indicated by visible particles, turbidity or inappropriate blank absorbance at 340 nm. * * * # Urea UV vs Urea Berthelot Feature Urea UV Urea Berthelot Method **GLDH** Berthelot Measurement **UV Fixed Time** Colorimetric Endpoint Wavelength **340 nm** 578 nm Reaction Direction Decreasing Increasing Sample Volume 10 µl 10 µl Standard 50 mg/dL 50 mg/dL Linearity 300 mg/dL 300 mg/dL Best For UV-capable chemistry analysers Colorimetric endpoint analysers Customers should select the method that matches their analyser setup and validated laboratory workflow. Swemed lists Urea UV and Urea Berthelot as separate products. * * * # Why Urea UV Testing Is Important Urea is a major end product of **protein metabolism**. It is formed in the liver from ammonia produced during amino-acid breakdown and is transported to the kidneys for excretion. Urea testing is commonly used in: - Renal-function evaluation - Kidney-disease assessment - Evaluation of urinary obstruction - Hydration-status assessment - General metabolic investigation - Routine health screening - Monitoring of selected acute and chronic conditions Urea results should be interpreted together with creatinine, electrolytes, clinical history and other relevant findings. * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine urea UV testing Diagnostic Centres Renal profile testing Hospital Laboratories Clinical chemistry testing Nephrology Centres Renal-function investigations Dialysis Centres Renal biochemical assessment Clinical Chemistry Laboratories Routine UV chemistry Medical Institutions Teaching and diagnostic use Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted * * * # Useful Tips Before Ordering - Confirm that **Urea UV / GLDH** is required rather than Urea Berthelot. - Select the correct pack size: **25 ml, 50 ml or 100 ml**. - Confirm your analyser supports **340 nm UV measurement**. - Verify the analyser can perform a **fixed-time assay**. - Confirm the programmed reagent and sample volumes. - Check the required **30-second delay and 60-second reaction time**. - Do not use ammonium-heparin plasma. - Use fresh, properly handled specimens. - Follow the 1:50 urine dilution where applicable. - Perform calibration and internal quality control before routine reporting. - Validate open-system analyser application before clinical use. * * * # Buy Swemed Urea UV Reagent in Bangalore Microsidd India supplies **renal-profile reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka**. The **Swemed Urea UV Reagent Kit** is available on microsidd.com in **25 ml, 50 ml and 100 ml variants** and is suitable for compatible open-system fully automatic and semi-automatic analysers. The product is available for professional laboratory procurement, subject to stock availability and delivery serviceability. * * * # Search Relevance Relevant search phrases include: - Urea UV reagent supplier in Bangalore - Swemed Urea UV Bangalore - Urea UV GLDH reagent Bengaluru - Urea reagent 25 ml - Urea UV reagent 50 ml - Urea UV reagent 100 ml - Urea reagent for semi auto analyser - Urea reagent for fully automatic analyser - Open system Urea UV reagent India - Renal profile reagent supplier Bangalore - Kidney function reagent supplier Bengaluru - Swemed diagnostics reagent supplier India - laboratory reagents sypplier in india * * * # Product Summary ### What is Swemed Urea UV Reagent? The **Swemed Urea UV Reagent** is a professional clinical biochemistry reagent for quantitative measurement of urea using the **GLDH enzymatic fixed-time method at 340 nm**. ### Which pack sizes are available? - **25 ml** - **50 ml** - **100 ml** ### What method does it use? **GLDH Method**. ### What wavelength is used? **340 nm**. ### What is the sample volume? **10 µl**. ### What is the reagent volume? **1000 µl**. ### What is the standard concentration? **50 mg/dL**. ### What is the assay linearity? **Up to 300 mg/dL**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the working reagent stable? - **5 days at 15–25°C** - **4 weeks at 2–8°C** ### Is it suitable for semi-auto analysers? Yes, when used on compatible **open-system semi-automatic analysers** after programming and validation. ### Is it suitable for fully automatic analysers? Yes, on compatible **open-system fully automatic analysers** supporting 340 nm fixed-time chemistry and third-party reagent programming. * * * # Entity Information Entity Details Product Urea UV Reagent Kit Brand Swemed Diagnostics Pack Sizes 25 ml / 50 ml / 100 ml Analyte Urea Method GLDH Mode Fixed Time Wavelength 340 nm Specimen Serum / Plasma / Urine Clinical Category Renal Profile Product Category Laboratory Reagents Subcategory Biochemistry Reagents Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Analysers Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com * * * # Why Buy Swemed Urea UV Reagent from Microsidd.com? Urea reagents need to be selected carefully because **Urea UV and Urea Berthelot are different methodologies** and analyser compatibility matters. Microsidd India focuses on professional laboratory, diagnostic and healthcare products and has long-standing experience in the IVD sector. This helps laboratory buyers verify the **correct parameter, method, pack size and open-system application** before ordering. ## Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic & laboratory focused Seller expertise varies Dealer dependent Method Verification Urea UV vs Berthelot can be checked Similar listings may confuse buyers Depends on supplier Pack Variants 25 ml / 50 ml / 100 ml listed clearly Listing may vary Stock dependent Open-System Guidance Suitable for analyser-specific queries Often limited Dealer dependent Seller Identity Direct Microsidd India purchase Marketplace seller may vary Usually identifiable GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for professional labs Seller dependent Usually local Product Range Specialist biochemistry catalogue Broad but not specialised Often limited Direct Support Microsidd support Marketplace + third-party seller Local support India-Wide Supply Subject to serviceability Broad Usually regional # Frequently Asked Questions ### What is Swemed Urea UV Reagent used for? It is used for quantitative determination of urea in **serum, plasma and urine** using the GLDH method. ### What pack sizes are available? This Microsidd listing includes: **25 ml, 50 ml and 100 ml**. ### What method does the reagent use? **GLDH Method**. ### What wavelength is used? **340 nm**. ### What is the assay mode? **Fixed Time**. ### What is the standard concentration? **50 mg/dL**. ### What is the sample volume? **10 µl**. ### What is the reaction time? **60 seconds**, after a **30-second delay**. ### What is the linearity? The assay is linear up to **300 mg/dL**. ### Can urine be tested? Yes. Fresh urine should be diluted **1 + 49 with distilled water**, and the final result multiplied by 50. ### Can it be used on semi-automatic analysers? Yes, on compatible open-system semi-automatic biochemistry analysers after programming and validation. ### Can it be used on fully automatic analysers? Yes, on compatible open-system fully automatic analysers that support 340 nm fixed-time measurement and third-party reagent programming. ### Is Urea UV the same as Urea Berthelot? No. Urea UV uses **GLDH and UV measurement at 340 nm**, while Urea Berthelot is a colorimetric endpoint method at 578 nm. * * * # Medical and Laboratory Disclaimer The information on this page is provided for **product identification, procurement and professional laboratory education only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. Urea results should not be interpreted independently to diagnose or exclude kidney disease or any other medical condition. Results should be correlated with creatinine, electrolytes, clinical history and other relevant investigations. For open-system fully automatic and semi-automatic analysers, **analyser compatibility, application settings, calibration and performance must be verified and validated by the laboratory before routine patient testing**. The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 500.0 INR - 2000.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | 25ml | 500.00 INR | In stock | | 50ml | 1000.00 INR | In stock | | 100ml | 2000.00 INR | In stock | ## Images - Swemed Urea UV Reagent | 25ml, 50ml, 100ml | GLDH Method | Microsidd - Swemed Urea UV Reagent | 25ml, 50ml, 100ml | GLDH Method | Microsidd - Buy Swemed Urea UV Reagent from Microsidd.com - Urea UV laboratory reagents supplier in Bangalore - Microsidd India supplies renal-profile reagents, clinical biochemistry reagents and laboratory products from Bangalore --- > Source: [Microsidd India](https://microsidd.com/products/urea-uv-reagent) > Updated: 2026-09-13