# Urea Berthelot Reagent Kit 2 × 50 ml – Berthelot Method # Swemed Urea Berthelot Reagent Kit 2 × 50 ml The **Swemed Urea Berthelot Reagent Kit 2 × 50 ml** is a professional clinical biochemistry reagent intended for the **photometric quantitative determination of urea in serum, plasma and urine** using the established **Berthelot Method**. In this method, urease hydrolyses urea to produce ammonia. The ammonia subsequently reacts with a phenolic chromogen and hypochlorite to form a **green-coloured complex**. The intensity of the colour produced is directly proportional to the concentration of urea in the specimen. Swemed's current catalogue lists **Urea (Berthelot)** in **2 × 50 ml and 2 × 100 ml presentations**, with the Berthelot Method and a listed shelf life of 24 months. The **2 × 50 ml variant** is suitable for pathology laboratories, diagnostic centres, hospital laboratories and clinical chemistry facilities using compatible **open-system fully automatic or semi-automatic biochemistry analysers**. * * * # Product at a Glance Product Detail Information Product Name Urea Berthelot Reagent Kit Brand Swemed Diagnostics Pack Size **2 × 50 ml** Product Category Clinical Biochemistry Reagent Test Parameter Urea Method **Berthelot Method** Measurement Photometric / Colorimetric Mode **Endpoint** Wavelength **578 nm** Temperature **37°C** Standard Concentration **50 mg/dL** Reaction Direction Increasing Sample Volume **10 µl** Quick-Reference Reagent Volume **1000 µl** Linearity **Up to 300 mg/dL** Specimens Serum, Plasma, Urine Plasma Types Heparin / EDTA Reagent Preparation Ready to Use Storage **2–8°C** Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India Swemed specifies endpoint measurement at **578 nm**, a 50 mg/dL standard, 10 µl sample volume and linearity up to 300 mg/dL. * * * # Kit Components The Swemed Urea Berthelot system includes: Component Description R1 Buffer Reagent R2 Enzyme Reagent R3 Chromogen Reagent Standard Urea Berthelot Standard IFU Instructions for Use Swemed lists the **2 × 50 ml configuration as reference UB50** in its technical insert. * * * # Key Features - **Swemed Diagnostics Urea Berthelot Reagent Kit** - Pack size: **2 × 50 ml** - Established **Berthelot Method** - Endpoint colorimetric assay - Measurement wavelength: **578 nm** - Standard concentration: **50 mg/dL** - Reagents and standard supplied **ready to use** - Suitable for serum and plasma - Can also be used for urine according to manufacturer procedure - Linearity up to **300 mg/dL** - Suitable for compatible open-system semi-auto analysers - Suitable for compatible open-system fully automatic analysers - Reagents stored at **2–8°C** - For **professional in vitro diagnostic use only** * * * # Key Benefits ## Established Berthelot Chemistry The assay uses urease followed by the Berthelot colour reaction, providing a conventional enzymatic-colorimetric method for routine urea measurement. ## Ready-to-Use Reagents Swemed states that the **reagents and standard are ready to use**, reducing reagent-preparation steps during routine testing. ## Suitable for Renal Profile Testing Urea is one of the most frequently requested renal biochemistry parameters and is commonly tested alongside: - Creatinine - Uric Acid - Sodium - Potassium - Chloride - Calcium - Phosphorus - eGFR-related renal assessment ## Open-System Flexibility The reagent can be programmed on compatible **open-system fully automatic and semi-automatic analysers** that support endpoint photometric testing at 578 nm, subject to application validation by the laboratory. ## Useful Measuring Range The manufacturer states linearity up to **300 mg/dL**, suitable for routine clinical chemistry applications. * * * # Compatible with Open-System Biochemistry Analysers The **Swemed Urea Berthelot Reagent** is suitable for compatible programmable open-system analysers. Analyser Parameter Swemed Setting Method Endpoint Wavelength **578 nm** Temperature **37°C** Unit mg/dL Standard Concentration **50 mg/dL** Reaction Direction Increasing Sample Volume **10 µl** Reagent Volume **1000 µl** quick-reference setting Blank Reagent Blank Linearity **300 mg/dL** ### Suitable Analyser Types - Open-system semi-automatic biochemistry analysers - Open-system fully automatic biochemistry analysers - Programmable clinical chemistry systems supporting the required endpoint method **Analyser compatibility must be confirmed and the reagent application validated by the laboratory before reporting patient results.** * * * # Intended Use The **Swemed Urea Berthelot Reagent Kit** is intended for the **photometric quantitative determination of urea in serum, plasma and urine**. Swemed specifically lists: - Serum - Heparin plasma - EDTA plasma - Urine The product is intended for **professional in vitro diagnostic use only**. * * * # Test Principle Urease first hydrolyses urea: **Urea + H₂O → Ammonia + CO₂** The ammonia produced subsequently reacts with a **phenolic chromogen and hypochlorite**, producing a green-coloured complex. The intensity of the colour formed is directly proportional to the urea concentration in the specimen. * * * # Technical Specifications Parameter Manufacturer Specification Test Urea Berthelot Method Berthelot Mode Endpoint Wavelength **578 nm** Temperature **37°C** Unit mg/dL Standard **50 mg/dL** Reaction Slope Increasing Quick-Reference Reagent Volume **1000 µl** Sample Volume **10 µl** Blanking Reagent Blank Linearity **300 mg/dL** * * * # Manufacturer Assay Procedure For the manual procedure, Swemed specifies: Component Blank Standard Test R1 500 µl 500 µl 500 µl R2 50 µl 50 µl 50 µl Standard — 10 µl — Sample — — 10 µl Mix and incubate: **5 minutes at 37°C** or **10 minutes at room temperature** Then add: Component Blank Standard Test R3 500 µl 500 µl 500 µl Mix again and incubate for **5 minutes at 37°C or 10 minutes at room temperature**, then measure absorbance against the blank at **578 nm**. * * * # Calculation **Urea (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration** The manufacturer standard concentration is: **50 mg/dL** * * * # Specimen Requirements Swemed identifies the following acceptable specimens: - Serum - Heparin plasma - EDTA plasma The manufacturer recommends using **non-haemolysed serum or plasma**. Acceptable anticoagulants listed in the insert include: - Heparin - EDTA - Fluoride **Ammonium heparin should not be used**, because ammonium ions may falsely increase the result. * * * # Urine Testing The Swemed Urea Berthelot reagent can also be used for urine urea determination. According to the manufacturer, urine should be: **Pre-diluted 1:50 using ammonium-free water before testing.** * * * # Specimen Stability ## Serum / Plasma Storage Condition Stability 15–25°C **7 days** 2–8°C **7 days** −20°C **1 year** ## Urine Storage Condition Stability 15–25°C **2 days** 2–8°C **7 days** −20°C **1 month** * * * # Measuring Range and Linearity Swemed states that the Berthelot reaction is linear up to: **300 mg/dL** Samples above the linearity limit should be diluted **1 + 2 with physiological saline**, repeated and the obtained result multiplied by **3**. * * * # Interference and Important Precautions The manufacturer notes: - **Ammonium heparin must not be used** - Ammonium ions may cause falsely elevated results - Amines may suppress Berthelot colour development - Thiols may interfere - Steroids may interfere - Ascorbic acid may suppress colour development Proper sample collection and laboratory handling are therefore important. * * * # Manufacturer Reference Range Specimen Swemed Reference Serum / Plasma **10–50 mg/dL** Urine – 24 hours **20–35 g/24 h** Reference intervals can vary between laboratories and populations. Each laboratory should establish or verify its own appropriate reference interval. * * * # Storage and Stability Swemed states that the reagent and standard should be: - Stored at **2–8°C** - Protected from light - Protected from contamination - **Not frozen** - Used only within the stated expiry date Signs of reagent deterioration include: - Visible particles - Turbidity - Reagent blank absorbance at 578 nm **greater than 0.25** The Swemed catalogue lists a **24-month shelf life** for the Urea Berthelot product when properly stored. * * * # Why Urea Testing Is Important Urea is an end product of **protein metabolism**. It is formed primarily in the liver from ammonia generated during amino-acid metabolism and is transported in the bloodstream to the kidneys for excretion. Urea testing commonly forms part of: - Renal function evaluation - Kidney disease assessment - Investigation of urinary obstruction - Hydration assessment - Protein-metabolism evaluation - Routine health screening - Monitoring of selected critically ill patients - Renal profile testing Urea values should not be interpreted independently because concentration can be affected by renal function, hydration, dietary protein intake, liver function and other clinical factors. * * * # Urea in a Renal Profile Test Laboratory Relevance Urea Protein metabolism and renal excretion Creatinine Renal filtration assessment Uric Acid Purine metabolism Sodium Electrolyte and fluid balance Potassium Electrolyte and renal assessment Chloride Electrolyte / acid-base assessment Calcium Mineral metabolism Phosphorus Renal and mineral metabolism * * * # Urea Berthelot vs Urea UV Feature Urea Berthelot Urea UV Method Berthelot GLDH Measurement Colorimetric Endpoint UV Fixed-Time / Kinetic Typical Wavelength **578 nm** **340 nm** Swemed Berthelot Pack 2 × 50 ml / 2 × 100 ml Different pack configuration Application Routine colorimetric urea testing UV-based analyser testing Selection Depends on analyser and laboratory method Depends on analyser and laboratory method Swemed lists Berthelot and Urea UV as separate reagent products; customers should verify the required method before ordering. * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine urea testing Diagnostic Centres Renal profile testing Hospital Laboratories Inpatient and outpatient chemistry Nephrology Centres Renal-function investigations Dialysis Centres Renal biochemical assessment Clinical Chemistry Laboratories Routine urea estimation Medical Institutions Teaching and diagnostic laboratory use Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted * * * # Useful Tips Before Ordering - Confirm that **Urea Berthelot** is required rather than Urea UV. - Verify the pack size: **2 × 50 ml**. - Confirm analyser support for **578 nm endpoint measurement**. - Verify that the analyser is an **open system** before programming third-party reagent. - Confirm the sample requirement and reagent settings. - Do not use ammonium-heparin plasma. - Avoid haemolysed specimens. - Verify the urine dilution procedure if testing urine. - Store the reagents at **2–8°C**. - Do not freeze. - Perform calibration and internal quality control before routine reporting. * * * # Buy Swemed Urea Berthelot Reagent in Bangalore Microsidd India supplies **renal-profile reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka**. The **Swemed Urea Berthelot Reagent Kit 2 × 50 ml** is suitable for pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and professional clinical chemistry facilities. The product can be ordered through **microsidd.com**, subject to stock availability and delivery serviceability. * * * # Search Relevance This product is relevant for buyers searching for: - Urea Berthelot reagent supplier in Bangalore - Swemed Urea Berthelot Bangalore - Urea reagent 2 × 50 ml - Berthelot urea reagent Bengaluru - Urea reagent for semi auto analyser - Urea reagent for fully automatic analyser - Open system urea reagent India - Renal profile reagent supplier Bangalore - Kidney function reagent supplier Bengaluru - Clinical chemistry reagent supplier near me - Pathology lab reagent supplier Bangalore - Swemed Diagnostics Urea reagent online India * * * # Product Summary ### What is Swemed Urea Berthelot Reagent Kit? The **Swemed Urea Berthelot Reagent Kit 2 × 50 ml** is a professional clinical chemistry reagent used for quantitative photometric measurement of urea using the **Berthelot Method**. ### What pack size is available? This Shopify listing is for: **2 × 50 ml** Swemed also lists a 2 × 100 ml variant. ### What method does it use? It uses the **Berthelot Method**. ### What wavelength is used? The assay is measured at: **578 nm** ### What is the sample volume? The manufacturer specifies: **10 µl** ### What standard concentration is used? **50 mg/dL** ### What is the assay linearity? **Up to 300 mg/dL** ### Which specimens are suitable? - Serum - Heparin plasma - EDTA plasma - Urine according to the manufacturer procedure ### Is it suitable for semi-automatic analysers? Yes, for compatible **open-system semi-automatic biochemistry analysers** after programming and laboratory validation. ### Is it suitable for fully automatic analysers? Yes, for compatible **open-system fully automatic analysers** that support the required endpoint application and permit third-party reagent programming. ### Where can it be purchased? The product can be ordered from **Microsidd India through microsidd.com**, subject to stock availability. * * * # Entity Information Entity Details Product Urea Berthelot Reagent Kit Brand Swemed Diagnostics Pack Size 2 × 50 ml Test Parameter Urea Method Berthelot Measurement Colorimetric Endpoint Wavelength 578 nm Specimen Serum / Plasma / Urine Clinical Category Renal Profile Product Category Laboratory Reagents Subcategory Biochemistry Reagents Analyser Compatibility Open-System Fully Automatic & Semi-Automatic Biochemistry Analysers Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Hospital / Diagnostic Centre / B2B * * * # Why Buy Swemed Urea Berthelot Reagent from Microsidd.com? Clinical chemistry reagents require careful verification of the **parameter, methodology, pack size, analyser compatibility, storage requirements, expiry and seller source** before purchase. Microsidd India has long-standing experience in the IVD and diagnostic-products sector and focuses on laboratory, diagnostic and healthcare products. This gives professional laboratories an identifiable specialist procurement source rather than relying exclusively on general marketplace listings. ## Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic & laboratory focused Seller expertise varies Dealer dependent Product Understanding Suitable for reagent-specific enquiries Often general support Depends on supplier Seller Identity Direct Microsidd India purchase Marketplace and seller may differ Usually identifiable Method Verification Berthelot vs Urea UV can be checked Similar listings may confuse buyers Dealer dependent Pack Verification 2 × 50 ml can be confirmed Listing accuracy varies Depends on local stock Open-System Guidance Better suited to analyser-setting enquiries Often limited Dealer dependent GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for labs and institutions Seller dependent Usually local Product Range Specialist laboratory catalogue Broad but not specialised Usually limited Customer Support Direct Microsidd support Marketplace + seller layer Local support Seller Accountability Single identifiable specialist seller Seller may vary Supplier dependent India-Wide Supply Subject to serviceability Broad Usually regional # Frequently Asked Questions ### What is Swemed Urea Berthelot Reagent used for? It is used for quantitative determination of **urea in serum, plasma and urine** using the Berthelot Method. ### What is the pack size? **2 × 50 ml** ### Is Urea Berthelot the same as Urea UV? No. Berthelot is a **colorimetric endpoint method**, whereas Urea UV uses a GLDH-based UV method. ### What wavelength is required? **578 nm** ### What is the sample volume? **10 µl** ### What is the standard concentration? **50 mg/dL** ### What is the linearity? The assay is linear up to **300 mg/dL**. ### Are the reagents ready to use? Yes. Swemed states that the reagent and standard are **ready to use**. ### Can urine be tested? Yes. Swemed instructs laboratories to pre-dilute urine **1:50 with ammonium-free water** before assay. ### Can ammonium-heparin plasma be used? No. Ammonium heparin should not be used because ammonium ions may produce falsely elevated values. ### How should the reagent be stored? At **2–8°C**, protected from light and contamination. Do not freeze. ### Can it be used on a semi-auto analyser? Yes, when the analyser is an **open system** and supports the required endpoint settings. The application should be validated before routine use. ### Can it be used on a fully automatic analyser? Yes, on compatible **open-system fully automatic biochemistry analysers** that permit the required reagent programming. ### Is this suitable for home use? No. It is for **professional in vitro diagnostic use only**. ### Where can I buy Swemed Urea Berthelot Reagent in Bangalore? It can be purchased from **Microsidd India through microsidd.com**, subject to availability. * * * # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and laboratory educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. Urea results should not be interpreted independently to diagnose or exclude kidney disease or any other medical condition. Clinical interpretation may require creatinine, electrolytes, urine investigations, eGFR, patient history and other relevant investigations. The information provided by Microsidd India does not replace the current Swemed Diagnostics product insert, analyser instructions, laboratory SOPs, calibration requirements, quality-control procedures or professional medical judgement. For open-system fully automatic and semi-automatic analysers, **compatibility, reagent programming and application performance must be verified and validated by the laboratory before routine clinical reporting**. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 750.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 750.00 INR | In stock | ## Images - Buy Swemed Urea Berthelot Reagent in Bangalore - Buy Swemed Urea Berthelot Reagent from Microsidd.com - What is Swemed Urea Berthelot Reagent Kit - Swemed Diagnostics Urea reagent online India - Urea Berthelot reagent supplier in Bangalore --- > Source: [Microsidd India](https://microsidd.com/products/urea-berthelot-reagent-kit) > Updated: 2026-09-13