# Total Protein Reagent Kit 2 × 50 ml – Biuret Method # Swemed Total Protein Reagent Kit 2 × 50 ml The **Swemed Total Protein Reagent Kit 2 × 50 ml** is a professional clinical biochemistry reagent intended for the **photometric quantitative determination of total protein in serum and plasma** using the **Biuret Method**. Swemed lists the 2 × 50 ml pack as reference **TPR100**, with a second 2 × 100 ml presentation also available. In the Biuret reaction, proteins in an alkaline medium bind with cupric ions to form a **blue-violet coloured complex**. The intensity of the colour produced is directly proportional to the amount of protein present in the sample. The reagent is supplied as a **ready-to-use liquid reagent**, together with a Total Protein standard. It is suitable for routine use in pathology laboratories, hospitals, diagnostic centres and clinical chemistry laboratories. * * * # Product at a Glance Product Detail Information Product Name Total Protein Reagent Kit Brand Swemed Diagnostics Pack Size **2 × 50 ml** Manufacturer Reference **TPR100** Product Type Clinical Biochemistry Reagent Test Parameter Total Protein Method **Biuret Method** Measurement Photometric / Colorimetric Mode Endpoint Specimen Serum, Heparin Plasma, EDTA Plasma Wavelength **546 nm** Standard Concentration **4 g/dL** Reagent Volume **1000 µl** Sample Volume **20 µl** Incubation **5 minutes at room temperature** Reaction Direction Increasing Linearity **Up to 12 g/dL** Reagent Preparation Ready to Use Reagent Storage **15–30°C / Room Temperature** Standard Storage **2–8°C** Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India The analytical parameters above are taken from the Swemed Total Protein product literature. * * * # Kit Contents For the **2 × 50 ml Swemed Total Protein Kit**, the manufacturer lists: Component Quantity Total Protein Reagent **2 × 50 ml** Total Protein Standard **1 vial** Instructions for Use **1** The supplied standard has a concentration of **4 g/dL**. * * * # Key Features - **Swemed Diagnostics Total Protein Reagent Kit** - Pack size: **2 × 50 ml** - Reference: **TPR100** - Uses the established **Biuret Method** - Ready-to-use reagent and standard - Photometric endpoint assay - Recommended wavelength: **546 nm** - Standard concentration: **4 g/dL** - Suitable specimens: serum, heparin plasma and EDTA plasma - Linearity up to **12 g/dL** - Suitable for routine clinical chemistry - Suitable for open-system fully automatic analysers after application programming and validation - Suitable for open-system semi-automatic analysers after programming and validation - For **professional in vitro diagnostic use only** * * * # Compatible with Open-System Biochemistry Analysers The **Swemed Total Protein Reagent Kit** can be used with **open-system fully automatic and semi-automatic biochemistry analysers** that allow user programming of the required assay parameters. Typical analyser requirements include: Analyser Parameter Swemed Total Protein Setting Method Endpoint Wavelength 546 nm Reaction Increasing Reagent Volume 1000 µl Sample Volume 20 µl Standard 4 g/dL Incubation 5 minutes Blank Reagent Blank Unit g/dL The laboratory should confirm that its analyser supports these parameters and should validate the application according to the analyser manufacturer's instructions and the current Swemed IFU before reporting patient results. * * * # Key Benefits ## Ready-to-Use Reagent Swemed states that both the **reagent and standard are ready to use**, helping laboratories reduce reagent-preparation steps during routine testing. ## Established Biuret Method The Biuret method is widely used for routine total protein determination because proteins form a coloured complex with cupric ions in alkaline conditions. ## Suitable for Open Systems The defined endpoint settings make the reagent practical for laboratories using programmable **open-system semi-automatic and fully automatic biochemistry analysers**. ## Broad Routine Laboratory Application Total protein is frequently measured as part of: - Liver function profiles - General clinical chemistry panels - Nutritional assessment - Protein-status evaluation - Renal and systemic investigations ## Convenient 2 × 50 ml Pack The **2 × 50 ml pack** is practical for laboratories requiring a compact professional reagent configuration. * * * # Intended Use The **Swemed Total Protein Reagent Kit** is intended for the photometric quantitative determination of **total protein in serum and plasma** using the Biuret Method. Swemed lists the following suitable specimens: - Serum - Heparin plasma - EDTA plasma This product is intended for **professional in vitro diagnostic use only**. * * * # Test Principle Proteins contain peptide bonds that react with **cupric ions in an alkaline medium**. This reaction forms a **blue-violet copper-protein complex**. The intensity of the colour formed is proportional to the concentration of total protein in the specimen. * * * # Reagent Composition Component Manufacturer Specification Copper Sulphate 2 g/L Potassium Iodide 5 g/L Sodium Hydroxide 8 g/L Total Protein Standard 4 g/dL * * * # Technical Specifications Test Parameter Manufacturer Specification Parameter Total Protein Method Biuret Mode Endpoint Wavelength **546 nm** Unit **g/dL** Temperature 37°C Standard Concentration **4 g/dL** Reaction Slope Increasing Reagent Volume **1000 µl** Sample Volume **20 µl** Incubation Time **5 minutes at room temperature** Blanking Reagent Blank Linearity **12 g/dL** * * * # Assay Procedure Summary Tube Reagent Standard / Sample Blank 1000 µl — Standard 1000 µl 20 µl Standard Test 1000 µl 20 µl Sample Mix and incubate for **5 minutes at room temperature**, then read absorbance against the reagent blank at **546 nm**. ### Calculation **Total Protein (g/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration** The supplied standard concentration is **4 g/dL**. * * * # Specimen Requirements Swemed lists: - Serum - Heparin plasma - EDTA plasma Fresh, clear, **non-haemolysed serum from fasting patients** is recommended. The manufacturer warns that haemolysis may cause falsely elevated total protein results because released haemoglobin increases measured protein and background colour. Samples containing **bromsulfophthalein (BSP)** may also produce falsely elevated results. * * * # Specimen Stability According to the Swemed product literature: Storage Condition Stability Serum / Plasma at 2–8°C **6 days** Samples should be protected from bacterial contamination and evaporation. * * * # Measuring Range and Linearity The Swemed Total Protein assay is linear up to **12 g/dL**. For results above 12 g/dL, the manufacturer instructs laboratories to dilute the sample **1:1 with 0.9% NaCl solution**, repeat the assay and multiply the result by **2**. The manufacturer also states that the Biuret procedure is **not sufficiently sensitive at very low protein concentrations below 1 g/dL**, and this kit should **not be used for urine or cerebrospinal fluid protein testing**. * * * # Manufacturer Reference Range Specimen Manufacturer Reference Range Serum / Plasma **6.2–8.5 g/dL** Each laboratory should verify whether the manufacturer reference interval is suitable for its own patient population and analyser system. * * * # Storage and Stability The manufacturer states: - Reagent: stable at **15–30°C / room temperature** - Standard: store at **2–8°C** - Protect from light - Protect from contamination - **Do not freeze** - Bring standard and specimens to room temperature before use Signs of possible reagent deterioration include: - Visible particles - Turbidity - Reagent blank absorbance at 546 nm above **0.22** * * * # Why Total Protein Testing Is Important Proteins are essential structural and functional components of the body and are involved in: - Muscle structure - Enzymes - Hormones - Transport functions - Maintenance of oncotic pressure - Distribution of water between blood and tissues Changes in total protein concentration may be associated with conditions such as dehydration, impaired protein synthesis, malnutrition, protein loss and increased protein breakdown. Total protein results should always be interpreted together with the patient's clinical history and related tests. * * * # Total Protein in a Clinical Chemistry Profile Related Parameter Laboratory Relevance Total Protein Overall serum protein concentration Albumin Major circulating plasma protein Globulin Often calculated from Total Protein – Albumin A/G Ratio Albumin-to-globulin relationship Bilirubin Liver-related assessment SGOT / AST Liver and tissue enzyme SGPT / ALT Liver-associated enzyme Alkaline Phosphatase Hepatobiliary / bone-related enzyme Creatinine Renal-function assessment Urea Renal and protein-metabolism assessment * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine total protein testing Diagnostic Centres Clinical chemistry testing Hospital Laboratories Inpatient and outpatient investigations Liver / Gastroenterology Centres Liver-function profiles Nutrition Clinics with Laboratories Protein-status assessment Medical Institutions Diagnostic and teaching laboratory use Clinical Chemistry Laboratories Routine biochemical testing Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted * * * # Useful Tips Before Ordering - Confirm that **Total Protein – Biuret Method** is required. - Verify the pack size: **2 × 50 ml**. - Confirm that your analyser is an **open system** if you plan to programme the reagent manually. - Ensure the analyser supports **546 nm endpoint testing**. - Verify the laboratory's programmed reagent and sample volumes. - Confirm the supplied standard concentration: **4 g/dL**. - Use serum, heparin plasma or EDTA plasma. - Avoid haemolysed specimens. - Do not use this reagent for urine or CSF total protein testing. - Store reagent and standard according to manufacturer instructions. - Perform calibration and internal quality control before reporting patient results. * * * # Buy Swemed Total Protein Reagent in Bangalore Microsidd India supplies **clinical biochemistry reagents, liver-function reagents and laboratory products from Bangalore / Bengaluru, Karnataka**. The **Swemed Total Protein Reagent Kit 2 × 50 ml** is suitable for pathology laboratories, diagnostic centres, hospitals, clinical chemistry laboratories and institutional healthcare buyers. It can be ordered through **microsidd.com**, subject to stock availability and delivery serviceability. * * * # Search Relevance This product is relevant for customers searching for: - Total Protein reagent supplier in Bangalore - Swemed Total Protein Reagent Bangalore - Total Protein Biuret reagent Bengaluru - Total Protein reagent 2 × 50 ml - Biuret reagent supplier India - Total Protein reagent for semi auto analyser - Total Protein reagent for fully automatic analyser - Open system biochemistry reagent Bangalore - Clinical chemistry reagent supplier near me - Pathology lab reagent supplier Bangalore - Swemed Total Protein kit online India - Swemed Diagnostics reagent supplier India * * * # Product Summary ### What is Swemed Total Protein Reagent Kit 2 × 50 ml? The **Swemed Total Protein Reagent Kit 2 × 50 ml** is a ready-to-use professional clinical chemistry reagent used for photometric quantitative determination of total protein using the **Biuret Method**. ### Which specimens can be used? Swemed lists: - Serum - Heparin plasma - EDTA plasma ### What wavelength is used? The assay is performed at **546 nm**. ### What is the standard concentration? The supplied Total Protein standard concentration is **4 g/dL**. ### What is the sample volume? The manufacturer procedure uses **20 µl sample** with **1000 µl reagent**. ### What is the assay linearity? The stated linearity is **12 g/dL**. ### Is the reagent ready to use? Yes. Swemed states that the **reagent and standard are ready to use**. ### Is Swemed Total Protein compatible with fully automatic analysers? It can be used on **open-system fully automatic analysers** that allow programming of the required endpoint method, wavelength and reagent/sample volumes, subject to analyser compatibility and laboratory validation. ### Is it compatible with semi-automatic analysers? Yes, it is suitable for compatible **open-system semi-automatic biochemistry analysers** after appropriate programming and validation. ### Can this kit be used for urine protein? No. The manufacturer states that this Biuret assay is not suitable for low protein concentrations below 1 g/dL and should **not be used for urine or spinal fluid testing**. ### Where can it be purchased? The product can be ordered from **Microsidd India through microsidd.com**, subject to stock availability and serviceability. * * * # Entity Information Entity Details Product Total Protein Reagent Kit Brand Swemed Diagnostics Pack Size 2 × 50 ml Manufacturer Reference TPR100 Analyte Total Protein Method Biuret Test Type Photometric Endpoint Specimen Serum / Heparin Plasma / EDTA Plasma Wavelength 546 nm Category Laboratory Reagents Subcategory Biochemistry Reagents Clinical Category Liver Function / General Clinical Chemistry Analyser Compatibility Open-System Fully & Semi Automatic Biochemistry Analysers Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Hospital / Diagnostic Centre / B2B # Why Buy Swemed Total Protein Reagent from Microsidd.com? Laboratory reagents should be purchased with attention to much more than price. The laboratory needs confidence regarding the **correct method, pack size, standard concentration, analyser compatibility, reagent storage, expiry, product source and seller accountability**. Microsidd India has long-standing experience in the **IVD and diagnostic-products sector** and focuses specifically on laboratory, diagnostic and healthcare products. Professional buyers therefore purchase through an identifiable specialist diagnostic supplier rather than relying only on a general marketplace seller. * * * # Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic and laboratory focused Seller expertise varies Depends on dealer Product Understanding Better suited to reagent-specific enquiries Often general marketplace support Dealer dependent Seller Identity Direct purchase from Microsidd India Marketplace and seller may differ Usually locally identifiable Pack Verification 2 × 50 ml pack can be checked before ordering Similar listings may create confusion Depends on local stock Method Verification Biuret methodology can be confirmed Seller dependent Dealer dependent Analyser Compatibility Guidance Better suited to open-system reagent queries Often limited Depends on dealer knowledge GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for laboratories and institutions Seller dependent Usually local Product Range Specialist diagnostic and laboratory catalogue Broad but not specialised Usually limited Customer Support Direct Microsidd support Marketplace plus seller layer Local support Accountability One identifiable specialist supplier Seller may vary between listings Depends on supplier India-Wide Supply Subject to serviceability Broad Usually regional * * * # Benefits of Buying from Microsidd.com Microsidd Advantage Benefit to Laboratory Buyers Diagnostic Product Specialisation Better understanding of professional laboratory requirements Long-Standing IVD Experience Familiarity with diagnostic and clinical chemistry products Direct Seller Accountability Purchase directly from Microsidd India Product Verification Pack, method and technical specifications can be checked Open-System Analyser Support Easier verification of programmable reagent parameters GST Invoice Useful for laboratories and institutional procurement Multi-Category Procurement Liver, renal, lipid, cardiac and other reagents can be sourced together Bangalore-Based Operations Convenient for Bengaluru and Karnataka laboratories India-Wide Supply Delivery to serviceable locations across India Direct Customer Support Order and product queries handled directly * * * # Frequently Asked Questions ### What is Swemed Total Protein Reagent used for? It is used for quantitative measurement of **total protein in serum and plasma** by the Biuret Method. ### What is the pack size? The pack size is **2 × 50 ml**. ### What method does the kit use? It uses the **Biuret Method**. ### What is the wavelength? The assay is measured at **546 nm**. ### What is the standard concentration? The standard is **4 g/dL**. ### Which specimen can be used? Serum, heparin plasma and EDTA plasma. ### What is the sample volume? The assay uses **20 µl sample**. ### What is the reagent volume? The manufacturer procedure uses **1000 µl reagent**. ### What is the incubation time? The assay requires **5 minutes at room temperature**. ### What is the linearity? The stated linearity is **12 g/dL**. ### Is the reagent ready to use? Yes. The reagent and standard are ready to use. ### Can it be used on a semi-auto biochemistry analyser? Yes, if the analyser is an **open system** and supports the required endpoint method, 546 nm wavelength and programmable reagent/sample settings. ### Can it be used on a fully automatic biochemistry analyser? Yes, for **open-system fully automatic analysers** that allow reagent application programming. The laboratory must validate the method before routine reporting. ### Can I use this reagent for urine total protein? No. Swemed states that the Biuret procedure should not be used for urine or spinal-fluid protein testing because it is not sensitive enough at very low protein levels. ### How should the reagent be stored? The reagent is stored at **15–30°C**, while the standard is stored at **2–8°C**. Do not freeze. ### Where can I buy Swemed Total Protein Reagent in Bangalore? The product can be ordered from **Microsidd India through microsidd.com**, subject to availability. ### Can laboratories outside Bangalore order it? Yes. Microsidd supplies laboratory and diagnostic products to serviceable locations across India. * * * # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. Total protein results should not be interpreted independently to diagnose or exclude liver disease, nutritional disorders, kidney disease or other medical conditions. Results should be correlated with albumin, globulin, liver-function tests, renal-function tests, clinical history and other relevant investigations. The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement. For open-system fully automatic and semi-automatic analysers, **analyser compatibility and application settings must be verified and validated by the laboratory before routine clinical use**. Specimen requirements, reagent preparation, analyser settings, standardisation, reference intervals, storage, stability and interpretation should always follow the current manufacturer instructions and validated laboratory procedures. * * * # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 500.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 500.00 INR | In stock | ## Images - Swemed Total Protein Reagent 2x50ml Biuret Method Microsidd - Swemed Total Protein Reagent 2x50ml Biuret Method Microsidd - Swemed Total Protein Reagent 2x50ml Biuret Method Microsidd - Swemed Total Protein Reagent 2x50ml Biuret Method Microsidd - Swemed Total Protein Reagent 2x50ml Biuret Method Microsidd --- > Source: [Microsidd India](https://microsidd.com/products/total-protein-reagent-kit) > Updated: 2026-09-12