# LDH Reagent Kit 25 ml | Optimized DGKC Method # Swemed LDH Reagent Kit 25 ml The **Swemed LDH Reagent Kit 25 ml** is a professional clinical biochemistry reagent intended for the **quantitative determination of Lactate Dehydrogenase (LDH) activity** in suitable laboratory specimens. Swemed identifies its LDH assay as an **Optimized DGKC method** using the **pyruvate-to-lactate reaction**, with a kinetic measurement at **340 nm**. The reagent system is liquid stable, ready to use, and designed for routine professional clinical chemistry laboratories. This 25 ml pack is suitable for pathology laboratories, diagnostic centres, hospitals and clinical chemistry facilities using compatible **open-system semi-automatic and fully automatic biochemistry analysers**, subject to application programming and laboratory validation. # Product at a Glance Product Detail Information Product Name LDH Reagent Kit Brand Swemed Diagnostics Pack Size **25 ml** Test Parameter Lactate Dehydrogenase / LDH Method **Optimized DGKC** Assay Type **Kinetic UV** Reaction Pyruvate → Lactate Wavelength **340 nm** Temperature **37°C** Unit **U/L or IU/L** Reaction Direction **Decreasing** Reagent Volume **1000 µl** Sample Volume **25 µl** Delay Time **60 sec** Reaction Time **180 sec** Delta Time **60 sec** Factor **8199** Blanking Water Blank Manufacturer Insert Linearity **Up to 1000 U/L** Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Swemed's LDH insert specifies kinetic mode, 340 nm, 37°C, a factor of 8199, 1000 µl reagent, 25 µl sample, 60-second delay, 180-second reaction time and linearity to 1000 U/L. # Key Features - **Swemed Diagnostics LDH Reagent Kit** - Pack size: **25 ml** - Optimized DGKC methodology - Kinetic UV assay - Measures LDH activity through pyruvate-to-lactate reaction - Measurement wavelength: **340 nm** - Reaction direction: **Decreasing** - Sample volume: **25 µl** - Reagent volume: **1000 µl** - Factor: **8199** - Manufacturer insert linearity: **up to 1000 U/L** - Liquid-stable reagent system - Suitable for professional clinical chemistry laboratories - Compatible with suitable open-system semi-auto and fully automatic analysers - For professional in-vitro diagnostic use only Swemed's current catalogue additionally describes the LDH reagent as having **high working-reagent stability up to 30 days**, and lists a convenient **25 ml pack size**. # What is LDH? **Lactate Dehydrogenase (LDH)** is an intracellular enzyme involved in the reversible conversion between lactate and pyruvate. LDH is widely distributed in tissues including: - Heart - Liver - Skeletal muscle - Kidney - Blood cells - Other metabolically active tissues Because LDH occurs in many tissues, elevated LDH activity is **not disease-specific** and should always be interpreted with other laboratory findings and clinical information. # Test Principle The Swemed LDH method is based on the conversion of **pyruvate to lactate**. During the reaction, **NADH is oxidised to NAD+**. The decrease in NADH absorbance at **340 nm** is measured kinetically. The rate of decrease in absorbance is proportional to the LDH activity present in the sample. Swemed's catalogue specifically describes its LDH assay as an **Optimized DGKC method with pyruvate-to-lactate test reaction**. # Technical Specifications Parameter Swemed Specification Test LDH Method Optimized DGKC Mode Kinetic Wavelength **340 nm** Temperature **37°C** Unit **IU/L** Factor **8199** Reaction Slope **Decreasing** Reagent Volume **1000 µl** Sample Volume **25 µl** Delay Time **60 sec** Reaction Time **180 sec** Delta Time **60 sec** Blanking Water Blank Linearity **1000 U/L** # Assay Procedure Summary Swemed's LDH insert provides the following macro application: Component Volume Reagent R1 **800 µl** Reagent R2 **200 µl** Sample **25 µl** Mix well and read the initial absorbance after the specified delay, then continue absorbance readings at one-minute intervals. The average absorbance change per minute is used to calculate LDH activity. # Calculation For the Swemed application: **LDH activity = ΔA/min × Factor** Manufacturer factor: **8199** The exact analyser application should be confirmed using the current Swemed insert supplied with the reagent lot. # Measuring Range and Linearity Swemed's LDH insert states that the assay is linear up to: **1000 U/L** For specimens above the validated range, the manufacturer instructs dilution **1 + 5 with physiological saline**, repeating the test and multiplying the result by **6**. Swemed's newer product catalogue describes LDH as having high linearity up to **1200 IU/L**. Because manufacturer documents differ, laboratories should follow the **current lot-specific IFU supplied with the product** as the definitive specification. # Reference Range Swemed's LDH insert provides a serum reference range of: **230–460 U/L** The manufacturer also recommends that each laboratory establish or verify its own reference interval according to its patient population, analyser and analytical system. # Compatible with Open-System Biochemistry Analysers The **Swemed LDH Reagent Kit** can be used on compatible programmable open-system chemistry analysers that support kinetic UV measurement at **340 nm**. Suitable categories include: - Open-system semi-automatic biochemistry analysers - Open-system fully automatic biochemistry analysers - Programmable clinical chemistry platforms - UV-capable analysers supporting kinetic assays Typical application requirements include: Setting LDH Application Mode Kinetic Wavelength **340 nm** Temperature **37°C** Sample **25 µl** Reagent **1000 µl** Delay **60 sec** Reaction Time **180 sec** Reaction Direction Decreasing Factor **8199** The laboratory must validate the method on its own analyser before routine patient reporting. # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine enzyme testing Diagnostic Centres Clinical chemistry Hospital Laboratories LDH estimation Cardiology Laboratories Enzyme profile testing Hepatology Laboratories Clinical chemistry investigations Oncology Laboratories General biochemical monitoring Clinical Chemistry Labs Kinetic UV enzyme assays Medical Institutions Teaching and diagnostic use Healthcare Organisations Institutional procurement Laboratory Distributors Professional reagent supply # Useful Tips Before Ordering - Confirm that you require **LDH – Optimized DGKC Method**. - Verify pack size: **25 ml**. - Confirm analyser support for **340 nm kinetic UV measurement**. - Verify that your analyser allows open-system reagent programming. - Confirm sample volume: **25 µl**. - Confirm reagent volume: **1000 µl**. - Verify the factor and timing parameters before use. - Confirm current linearity from the supplied package insert. - Maintain appropriate internal quality control. - Check expiry and storage requirements before routine use. # Buy Swemed LDH Reagent in Bangalore Microsidd India supplies **LDH reagents, cardiac enzyme reagents and clinical biochemistry products from Bangalore / Bengaluru, Karnataka**. The **Swemed LDH Reagent Kit 25 ml** is suitable for pathology laboratories, diagnostic centres, hospital laboratories and clinical chemistry facilities using compatible open-system analysers. 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The **Swemed LDH Reagent Kit 25 ml** is a professional clinical chemistry reagent used for quantitative measurement of Lactate Dehydrogenase activity. ### What method does it use? **Optimized DGKC Method.** ### What assay type is used? A **kinetic UV assay**. ### What wavelength is required? **340 nm.** ### What is the sample volume? **25 µl.** ### What is the reagent volume? **1000 µl.** ### What is the reaction direction? **Decreasing.** ### What is the factor? **8199.** ### What is the assay linearity? The manufacturer insert states **1000 U/L**, while the newer catalogue describes high linearity up to **1200 IU/L**. Follow the current lot-specific IFU for final application parameters. ### Can it be used on semi-automatic analysers? Yes, on compatible open-system semi-automatic biochemistry analysers after method programming and validation. ### Can it be used on fully automatic analysers? Yes, on compatible open-system fully automatic chemistry analysers supporting the required kinetic UV application. # Why Buy Swemed LDH Reagent from Microsidd.com? Laboratory reagents should be purchased only after confirming the **correct analyte, methodology, pack size, wavelength, analyser compatibility and assay parameters**. Microsidd India focuses on professional diagnostic, laboratory and IVD products and provides Swemed reagents through dedicated Swemed and Biochemistry collections, making related reagent procurement easier for laboratories. Buying Factor Microsidd.com General Online Marketplaces Offline Suppliers Diagnostic Specialisation Laboratory / IVD focused Seller expertise varies Dealer dependent Method Verification Optimized DGKC clearly identified Listing dependent Depends on dealer Pack Verification 25 ml clearly listed May vary Stock dependent Open-System Guidance Suitable for analyser-specific queries Often limited Depends on expertise Related Swemed Reagents Easy cross-navigation Often scattered Stock dependent GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for labs and institutions Seller dependent Usually suitable Seller Identity Microsidd India Marketplace seller may vary Usually identifiable # Frequently Asked Questions ### What is Swemed LDH Reagent used for? It is used for quantitative determination of **Lactate Dehydrogenase enzyme activity** in professional clinical chemistry laboratories. ### What method is used? **Optimized DGKC Method.** ### What is the pack size? **25 ml.** ### What wavelength is required? **340 nm.** ### What is the assay mode? **Kinetic.** ### What is the sample volume? **25 µl.** ### What is the reagent volume? **1000 µl.** ### What is the factor? **8199.** ### What is the reaction direction? **Decreasing.** ### What is the manufacturer-stated linearity? The technical insert states **1000 U/L**, while the current catalogue describes up to **1200 IU/L**. Use the current lot-specific manufacturer IFU as the definitive value. ### Can it be used on semi-auto analysers? Yes, on compatible **open-system semi-automatic biochemistry analysers** after programming and validation. ### Can it be used on fully automatic analysers? Yes, on compatible open-system fully automatic analysers supporting the required kinetic UV method. ### Is LDH specific to one disease? No. LDH is present in multiple tissues, so an abnormal value should be interpreted with other clinical and laboratory findings. ### Is this product for home use? No. It is intended for **professional in-vitro diagnostic laboratory use only**. # Medical and Laboratory Disclaimer The information on this page is provided for **product identification, laboratory procurement and professional educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. LDH is a non-specific enzyme marker and should not be interpreted independently to diagnose or exclude cardiac, hepatic, haematological, muscular, oncological or other disorders. For open-system fully automatic and semi-automatic analysers, **instrument compatibility, reagent application settings, quality control and analytical performance must be independently verified and validated by the laboratory before routine patient reporting**. Because manufacturer documentation currently shows a difference between the technical insert and newer catalogue for maximum linearity, the **current package insert supplied with the actual reagent lot should be treated as the definitive technical specification**. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 850.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 850.00 INR | In stock | ## Images - Swemed LDH Reagent 25ml Optimized DGKC Method Microsidd - Swemed LDH Reagent 25ml Optimized DGKC Method Microsidd - Swemed LDH Reagent 25ml Optimized DGKC Method Microsidd - Swemed LDH Reagent 25ml Optimized DGKC Method Microsidd - Swemed LDH Reagent 25ml Optimized DGKC Method Microsidd --- > Source: [Microsidd India](https://microsidd.com/products/swemed-ldh-reagent) > Updated: 2026-09-14