# Alkaline Phosphatase Reagent Kit 25 mL | PNPP Kinetic Method

# SWEMED Alkaline Phosphatase Reagent Kit 25 mL | PNPP Kinetic Method

The **SWEMED Alkaline Phosphatase Reagent Kit – 25 mL** is a professional clinical biochemistry reagent intended for the **quantitative determination of alkaline phosphatase (ALP) activity in human serum** using the **PNPP kinetic method**.

The 25 mL kit is supplied as **20 mL of Buffer Reagent (R1) + 5 mL of Substrate Reagent (R2)** and is designed for routine use in clinical laboratories, hospitals, diagnostic centres and pathology laboratories.

# Product Information

**Product Name:** Alkaline Phosphatase Reagent Kit  
**Brand:** SWEMED  
**Manufacturer:** SWEMED Biomedicals Pvt. Ltd.  
**Product Category:** Laboratory Reagent / Clinical Chemistry Reagent  
**Subcategory:** Biochemistry Reagent  
**Test Parameter:** Alkaline Phosphatase (ALP)  
**Methodology:** PNPP Kinetic Method  
**Product Code:** ALP 702  
**Pack Reference:** ALKP25  
**Pack Size:** 25 mL  
**Pack Configuration:** R1 20 mL + R2 5 mL  
**Sample Type:** Serum  
**Measurement Type:** Quantitative ALP Activity Determination  
**Reagent Type:** Two-Reagent System  
**Intended Use:** Professional in-vitro diagnostic laboratory testing  
**Suitable Users:** Diagnostic laboratories, hospitals, pathology laboratories and clinical chemistry laboratories  
**Storage:** 2–8°C for reagent kit, protect from light  
**Shelf Life:** Manufacturer catalogue states 24 months  
**Seller:** Microsidd India

# What Is SWEMED Alkaline Phosphatase Reagent Kit?

The **SWEMED Alkaline Phosphatase Reagent Kit** is an in-vitro diagnostic reagent used for the **quantitative measurement of alkaline phosphatase enzyme activity in human serum**.

Alkaline phosphatase is an enzyme found in several tissues, with important concentrations in the **liver, biliary tract and bone**. Measurement of ALP is commonly included in routine clinical chemistry testing, especially in the assessment of hepatobiliary and bone-related conditions.

The SWEMED assay uses the **PNPP kinetic method**, in which alkaline phosphatase hydrolyses **p-nitrophenyl phosphate (PNPP)** under alkaline conditions to form p-nitrophenol. The increase in absorbance is proportional to the ALP activity in the sample.

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# Product Uses

The SWEMED Alkaline Phosphatase Reagent Kit is intended for:

-   Quantitative ALP activity measurement
-   Routine clinical chemistry testing
-   Liver function testing
-   Hepatobiliary assessment
-   Bone-related biochemical investigations
-   Hospital laboratory testing
-   Diagnostic laboratory testing
-   Pathology laboratory applications
-   Professional IVD use with compatible chemistry analysers

ALP results should be interpreted alongside other clinical information and related laboratory parameters.

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# Key Benefits & Features

### PNPP Kinetic Method

Uses a **PNPP kinetic assay** for quantitative determination of alkaline phosphatase activity.

### 25 mL Professional Pack

The 25 mL kit contains:

**R1 Buffer Reagent: 1 × 20 mL**  
**R2 Substrate Reagent: 1 × 5 mL**

### Suitable for Routine Clinical Chemistry

Designed for use in professional laboratory environments performing routine biochemistry testing.

### Two-Reagent System

The working reagent is prepared by mixing **4 parts of R1 with 1 part of R2**, according to the manufacturer’s IFU.

### Defined Working-Reagent Stability

SWEMED states working-reagent stability of approximately:

**5 days at 15–25°C**  
**2 weeks at 2–8°C**

### Manufacturer-Listed 24-Month Shelf Life

SWEMED’s current catalogue lists **24 months shelf life** for the Alkaline Phosphatase reagent kit.

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# How Does the SWEMED ALP Reagent Work?

The assay is based on the enzymatic hydrolysis of **p-nitrophenyl phosphate (PNPP)**.

Under alkaline conditions:

**PNPP + H₂O → p-Nitrophenol + Phosphate**

This reaction is catalysed by alkaline phosphatase present in the patient sample.

The rate at which p-nitrophenol forms is measured by the analyser as an increase in absorbance. The rate of absorbance change is proportional to the ALP activity in the serum specimen.

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# Who Is This Product For?

This reagent is intended for trained laboratory professionals working in:

-   Diagnostic laboratories
-   Pathology laboratories
-   Hospital laboratories
-   Clinical chemistry departments
-   Medical institutions
-   Healthcare laboratories
-   Other authorised IVD testing environments

It is **not intended for consumer home testing**.

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# What's Included?

### SWEMED Alkaline Phosphatase Kit – 25 mL

**ALKP-R1 Buffer Reagent:** 1 × 20 mL  
**ALKP-R2 Substrate Reagent:** 1 × 5 mL  
**Instructions for Use:** 1

Total reagent volume: **25 mL**.

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# How to Use

The reagent should be used according to the current **SWEMED Instructions for Use** and the operating requirements of the compatible biochemistry analyser.

The manufacturer specifies preparation of working reagent by mixing:

**4 parts R1 + 1 part R2**.

For routine laboratory use:

1.  Bring reagents and specimen to the required operating conditions.
2.  Prepare working reagent according to the SWEMED IFU.
3.  Load or dispense the working reagent and sample according to the analyser protocol.
4.  Perform kinetic absorbance measurement using the analyser’s validated ALP method parameters.
5.  Review results using the laboratory’s quality-control procedures.
6.  Record and report results according to laboratory SOPs.

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# Quick Facts

Information

Details

**Brand**

SWEMED

**Product**

Alkaline Phosphatase Reagent Kit

**Parameter**

ALP

**Method**

PNPP Kinetic

**Product Code**

ALP 702

**Pack Reference**

ALKP25

**Pack Size**

25 mL

**R1**

20 mL

**R2**

5 mL

**Sample**

Serum

**Reagent System**

Two-Reagent

**Storage**

2–8°C

**Shelf Life**

24 months, manufacturer-listed

**Use**

Professional IVD

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# Technical Specifications

Specification

Details

**Product Name**

Alkaline Phosphatase Reagent Kit

**Brand**

SWEMED

**Manufacturer**

SWEMED Biomedicals Pvt. Ltd.

**Analyte**

Alkaline Phosphatase

**Methodology**

PNPP Kinetic Method

**Product Code**

ALP 702

**Pack Reference**

ALKP25

**Pack Configuration**

R1 20 mL + R2 5 mL

**Total Volume**

25 mL

**Specimen**

Serum

**Measurement Type**

Quantitative Enzyme Activity

**Reagent Preparation**

4 parts R1 + 1 part R2

**Storage**

2–8°C

**Working Reagent Stability**

5 days at 15–25°C / 2 weeks at 2–8°C

**Product Type**

Clinical Biochemistry IVD Reagent

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# Storage & Handling

SWEMED specifies that the reagents are stable until the labelled expiry date when stored at **2–8°C**, protected from light and contamination. The substrate reagent is particularly light- and temperature-sensitive.

For proper handling:

-   Store the kit at **2–8°C**
-   Protect the substrate reagent from light
-   Do not freeze the reagents
-   Keep reagent caps tightly closed
-   Avoid contamination
-   Bring reagents and specimens to appropriate operating temperature before testing
-   Use clean laboratory tubes and pipette tips
-   Follow current GLP and laboratory biosafety procedures
-   Do not use after the labelled expiry date

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### Related SWEMED Product

**SWEMED Albumin Reagent Kit 2 × 50 mL [https://microsidd.com/products/swemed-albumin-reagent-kit-2x50ml](https://microsidd.com/products/swemed-albumin-reagent-kit-2x50ml "Microsidd Albumin reagent kit")**

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# Why Buy SWEMED Alkaline Phosphatase Reagent from Microsidd?

✓ SWEMED clinical biochemistry reagent  
✓ PNPP kinetic methodology  
✓ 25 mL professional laboratory pack  
✓ R1 + R2 reagent system  
✓ Suitable for routine ALP testing  
✓ Designed for professional clinical chemistry laboratories  
✓ Related SWEMED reagents available  
✓ Microsidd India – Bengaluru  
✓ Delivery to serviceable locations across India

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# Frequently Asked Questions

### What is SWEMED Alkaline Phosphatase Reagent Kit?

It is a professional clinical chemistry reagent for the **quantitative determination of alkaline phosphatase activity in human serum**.

### Which method does it use?

It uses the **PNPP Kinetic Method**.

### What is the pack size?

The pack contains **25 mL total reagent**.

### How is the 25 mL pack configured?

It contains **20 mL R1 Buffer Reagent + 5 mL R2 Substrate Reagent**.

### What is the product code?

SWEMED’s current catalogue lists **ALP 702**.

### What is the pack reference?

The IFU identifies the 25 mL configuration as **ALKP25**.

### What sample is required?

SWEMED’s IFU specifies **serum**.

### Can EDTA plasma be used?

The IFU specifically warns that **EDTA, oxalate and fluoride inhibit alkaline phosphatase** and are unsuitable anticoagulants for this assay.

### How is the working reagent prepared?

Mix **4 parts R1 with 1 part R2**.

### How long is the prepared working reagent stable?

SWEMED states approximately **5 days at 15–25°C** and **2 weeks at 2–8°C**.

### How should the kit be stored?

Store at **2–8°C**, protected from light and contamination. Do not freeze.

### What is the manufacturer-listed shelf life?

The current SWEMED catalogue states **24 months**.

### Is this reagent for home testing?

No. It is intended for **professional in-vitro diagnostic laboratory use**.

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# No Return / No Exchange Policy

**This product is non-returnable and non-exchangeable.**

Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**.

For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering.

Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law.

**An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.**

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# Professional / IVD Disclaimer

The **SWEMED Alkaline Phosphatase Reagent Kit is intended for professional in-vitro diagnostic use**.

Testing should be performed by trained laboratory personnel according to the manufacturer’s current Instructions for Use, compatible analyser specifications, laboratory SOPs and quality-control procedures.

ALP results should not be interpreted in isolation and should be considered together with other laboratory findings and appropriate clinical assessment.

## Details

- **Price:** 499.0 INR
- **Vendor:** Swemed Diagnostics
- **Type:** Biochemistry Reagents
- **Tags:** Bio Chemistry Reagents, biochemistry

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| Default Title | 499.00 INR | In stock |

## Images

- Alkaline Phosphatase Reagent Kit 25 mL | PNPP Kinetic Method
- SWEMED Alkaline Phosphatase Reagent 25mL PNPP Method Microsidd
- SWEMED Alkaline Phosphatase Reagent 25mL PNPP Method Microsidd
- SWEMED Alkaline Phosphatase Reagent 25mL PNPP Method Microsidd
- SWEMED Alkaline Phosphatase Reagent 25mL PNPP Method Microsidd

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> Source: [Microsidd India](https://microsidd.com/products/swemed-alkaline-phosphatase-reagent-kit-25ml)
> Updated: 2026-09-11