# SGPT (ALT) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method # Swemed SGPT (ALT) Liquid Stable Reagent Kit 50 ml The **Swemed SGPT (ALT) Liquid Stable Reagent Kit 50 ml** is a professional clinical biochemistry reagent intended for the quantitative determination of **Alanine Aminotransferase (ALT), also known as SGPT**, using the **Modified IFCC kinetic method**. Swemed’s official product literature describes the assay as a kinetic UV method in which ALT catalyses transfer of an amino group from L-alanine to α-ketoglutarate, forming pyruvate and L-glutamate. The pyruvate is then reduced by lactate dehydrogenase with simultaneous oxidation of NADH to NAD+, and the resulting **decrease in absorbance at 340 nm** is proportional to ALT activity. SGPT / ALT is one of the most commonly used enzymes in **liver function testing** and is generally considered more liver-associated than SGOT / AST. * * * ## Product at a Glance Product Detail Information Product Name SGPT / ALT Liquid Stable Reagent Kit Brand Swemed Diagnostics Pack Size **50 ml** Reagent R1 **40 ml** Reagent R2 **10 ml** Product Type Clinical Biochemistry Reagent Test Parameter SGPT / ALT Full Name Alanine Aminotransferase Method Modified IFCC Measurement Mode Kinetic UV Reaction Direction Decreasing Wavelength 340 nm Temperature 37°C Specimen Serum / Plasma Working Reagent Ratio 4 parts R1 + 1 part R2 Reagent Volume 1000 µl Sample Volume 100 µl Delay Time 60 seconds Reaction Time 180 seconds Reading Interval 60 seconds Calculation Factor 1746 Linearity Up to 300 U/L Storage 2–8°C Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India The assay parameters above are taken from the Swemed SGPT product insert; the **50 ml pack configuration with R1 40 ml + R2 10 ml** is the pack you are listing on Shopify. # Key Features - **Swemed Diagnostics SGPT / ALT Reagent Kit** - Total pack size: **50 ml** - **R1: 40 ml** - **R2: 10 ml** - **Modified IFCC kinetic method** - UV measurement at **340 nm** - Liquid stable two-reagent system - Suitable for professional SGPT / ALT activity measurement - Working reagent prepared in a **4:1 R1:R2 ratio** - Working reagent stability: **7 days at 15–25°C** - Working reagent stability: **4 weeks at 2–8°C** - Linearity up to **300 U/L** - Suitable for routine liver-function testing - For **professional in vitro diagnostic use only** # Key Benefits ## Modified IFCC Method The Swemed SGPT assay uses a **Modified IFCC kinetic method**, providing a standardised enzymatic approach for routine ALT activity measurement. ## Liquid Stable Reagent System The 50 ml kit contains **R1 40 ml + R2 10 ml**, giving the exact **4:1 reagent ratio** required for working-reagent preparation. ## Useful Working Reagent Stability The manufacturer states that prepared working reagent remains stable for: - **7 days at 15–25°C** - **4 weeks at 2–8°C** ## Suitable for Routine Liver Function Testing ALT / SGPT is commonly measured together with: - SGOT / AST - Bilirubin Total - Bilirubin Direct - Alkaline Phosphatase - Albumin - Total Protein - GGT where applicable ## Convenient 50 ml Pack The **40 ml R1 + 10 ml R2** configuration provides a compact pack for laboratories with routine or moderate SGPT testing volumes. # Intended Use The **Swemed SGPT / ALT Liquid Stable Reagent Kit** is intended for quantitative determination of **Alanine Aminotransferase activity in serum/plasma** using a Modified IFCC kinetic UV method. This product is intended for **professional in vitro diagnostic use only**. # Test Principle ALT catalyses: **L-Alanine + α-Ketoglutarate → L-Glutamate + Pyruvate** The pyruvate formed is then reduced by lactate dehydrogenase: **Pyruvate + NADH + H+ → L-Lactate + NAD+** The resulting **decrease in NADH absorbance at 340 nm** is directly proportional to SGPT / ALT activity in the sample. # Reagent Composition Component Manufacturer Specification Reagent 1 Tris Buffer pH 7.8 – 5 g/L Reagent 1 L-Alanine – 37.5 g/L Reagent 1 LDH – 4 kU/L Reagent 2 α-Ketoglutarate – 3.4 g/L Reagent 2 NADH – 1.1 g/L # Reagent Preparation Prepare the working reagent by mixing: **4 parts Reagent R1 + 1 part Reagent R2** For this 50 ml kit: **R1 40 ml + R2 10 ml** Swemed also states that **0.8 ml R1 + 0.2 ml R2** may be used directly instead of 1 ml pre-mixed working reagent during the assay. The working reagent should have an absorbance above **1.0 against distilled water at 340 nm**. # Working Reagent Stability Storage Condition Stability 15–25°C 7 days 2–8°C 4 weeks # Technical Specifications Test Parameter Manufacturer Specification Parameter SGPT / ALT Method Modified IFCC Mode Kinetic Wavelength 340 nm Unit IU/L Temperature 37°C Reaction Slope Decreasing Reagent Volume 1000 µl Sample Volume 100 µl Delay Time 60 seconds Reaction Time 180 seconds Reading Interval 60 seconds Blanking Water Blank Factor 1746 Linearity 300 U/L # Manual Assay Procedure Component Macro Method Semi-Micro Method R1 + R2 800 µl + 200 µl 400 µl + 100 µl Sample 100 µl 50 µl Mix well, read initial absorbance after **1 minute**, then read at 1-minute intervals for 3 minutes. Calculate the mean absorbance change per minute. ### Calculation **SGPT / ALT (U/L) = ΔA/min × 1746** # Measuring Range and Linearity The assay is linear up to **300 U/L**. If activity exceeds the stated range, Swemed recommends dilution **1 + 9 with 0.9% NaCl** and multiplication of the obtained result by **10**. # Specimen Requirements The manufacturer lists: - Serum - Plasma Fresh, clear, non-haemolysed serum from fasting patients is recommended. The insert also advises against using blood-collection devices lubricated with glycerin. # Specimen Stability According to the Swemed insert: Storage Condition Stability 15–30°C 3 days 2–8°C 7 days −20°C 30 days # Interference Information The manufacturer reports negligible interference under the evaluated conditions from: - Bilirubin up to **30 mg/dL** - Haemoglobin up to **400 mg/dL** # Manufacturer Reference Range Group Manufacturer Reference Women < 42 U/L Men < 32 U/L Each laboratory should establish or validate its own reference interval according to its patient population and analyser system. # Why SGPT / ALT Testing Is Important ALT is present in several tissues but is found in especially high concentrations in the **liver and kidneys**, and Swemed notes that ALT is generally considered more liver-specific than AST. SGPT / ALT testing may form part of investigations related to: - Hepatitis - Liver inflammation - Cirrhosis - Drug-induced liver injury - Metabolic liver disease - Routine liver function testing - Monitoring of selected systemic conditions ALT results should always be interpreted with clinical findings and related liver-function parameters. # SGPT / ALT in a Liver Function Profile Test Laboratory Relevance SGPT / ALT Liver-associated transaminase SGOT / AST Hepatic and extra-hepatic transaminase Bilirubin Total Bilirubin metabolism Bilirubin Direct Conjugated bilirubin assessment Alkaline Phosphatase Hepatobiliary and bone-associated enzyme Albumin Liver synthetic function Total Protein Protein status GGT Hepatobiliary enzyme where included # Storage and Handling Store the reagents: - At **2–8°C** - Protected from light - Protected from contamination - **Do not freeze** - Use only within the labelled shelf life Do not use reagent if the initial absorbance at **340 nm falls below 0.800**, or if the reagent fails to meet stated performance criteria. # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine ALT / SGPT testing Diagnostic Centres Liver function investigations Hospital Laboratories Clinical chemistry testing Gastroenterology Centres Liver enzyme assessment Hepatology Centres Liver function monitoring Clinical Chemistry Laboratories Routine enzyme testing Medical Institutions Diagnostic and teaching laboratories Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted # Useful Tips Before Ordering - Confirm that **SGPT / ALT** is the required parameter. - Verify the pack size: **50 ml**. - Confirm the configuration: **R1 40 ml + R2 10 ml**. - Ensure analyser support for **340 nm kinetic measurement**. - Confirm the laboratory uses a **Modified IFCC method**. - Verify analyser compatibility. - Store reagents at **2–8°C**. - Do not freeze. - Follow the **4:1 R1:R2 ratio**. - Follow current quality-control and calibration procedures. # Buy Swemed SGPT Reagent in Bangalore Microsidd India supplies **liver-function reagents, clinical chemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka**. The **Swemed SGPT / ALT Liquid Stable Reagent Kit 50 ml** with **R1 40 ml + R2 10 ml** is suitable for pathology laboratories, diagnostic centres, hospitals, liver clinics and professional clinical chemistry laboratories. Customers can order through **microsidd.com**, subject to stock availability and serviceability. # Search Relevance This product is relevant for buyers searching for: - SGPT reagent supplier in Bangalore - ALT reagent supplier Bengaluru - Swemed SGPT reagent Bangalore - SGPT reagent 50 ml - SGPT R1 40 ml R2 10 ml - ALT Modified IFCC reagent India - Liver function reagent supplier Bangalore - SGPT kinetic reagent supplier Karnataka - Clinical chemistry reagent supplier near me - Pathology lab reagent supplier Bangalore - SGPT test reagent online India - Swemed Diagnostics reagent supplier India # Product Summary ### What is Swemed SGPT Liquid Stable Reagent Kit 50 ml? The **Swemed SGPT / ALT Liquid Stable Reagent Kit 50 ml** is a professional clinical chemistry reagent used for quantitative ALT activity measurement by a **Modified IFCC kinetic UV method**. ### What is included in the 50 ml pack? - **R1: 40 ml** - **R2: 10 ml** ### What wavelength is used? The assay uses **340 nm**. ### What is the sample volume? The macro method uses **100 µl sample**; the semi-micro method uses **50 µl**. ### What is the assay factor? The factor is **1746**. ### What is the assay linearity? The stated linearity is **300 U/L**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the working reagent stable? - **7 days at 15–25°C** - **4 weeks at 2–8°C** ### Where can Swemed SGPT reagent be purchased? It can be ordered from **Microsidd India through microsidd.com**, subject to availability. # Entity Information Entity Details Product SGPT / ALT Reagent Kit Brand Swemed Diagnostics Pack Size 50 ml R1 40 ml R2 10 ml Analyte Alanine Aminotransferase Common Name SGPT / ALT Method Modified IFCC Measurement Kinetic UV Wavelength 340 nm Clinical Category Liver Function / Clinical Chemistry Product Category Laboratory Reagents Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Hospital / Diagnostic Centre / B2B # Why Buy Swemed SGPT Reagent from Microsidd.com? Clinical chemistry reagents should be purchased with careful attention to **method, pack configuration, analyser compatibility, storage requirements, expiry and seller credibility**. Microsidd India has long-standing experience in the **IVD and diagnostic-products sector** and focuses on laboratory, diagnostic and healthcare products rather than operating as a general-purpose marketplace. Professional buyers therefore deal with an identifiable specialist seller for recurring laboratory procurement. ## Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic and laboratory focused Seller expertise varies Depends on dealer Product Knowledge Better suited to reagent-specific queries Often general marketplace support Dealer dependent Seller Identity Direct purchase from Microsidd India Marketplace and seller may differ Usually locally identifiable Pack Verification **50 ml, R1 40 ml + R2 10 ml** can be verified before order Similar listings may cause confusion Depends on dealer knowledge Method Verification Modified IFCC can be confirmed Seller dependent Dealer dependent GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for labs, hospitals and institutions Seller dependent Usually local Product Range Specialist diagnostic and laboratory catalogue Broad but not specialised Usually limited by local stock Customer Support Direct Microsidd support Marketplace plus seller layer Local support Seller Accountability One identifiable specialist supplier Seller may change by listing Depends on supplier India-Wide Supply Subject to serviceability Broad Usually regional # Benefits of Buying from Microsidd.com Microsidd Advantage Benefit to Laboratory Buyers Diagnostic Product Specialisation Better suited to professional laboratory procurement IVD Sector Experience Greater familiarity with diagnostic products Direct Seller Accountability Purchase directly from Microsidd India GST Invoice Useful for professional buyers Product Verification Support Method and exact pack configuration can be checked Multi-Category Procurement Liver, renal, cardiac and lipid reagents can be sourced together Bangalore-Based Operations Convenient for Bengaluru and Karnataka laboratories India-Wide Supply Delivery to serviceable locations across India Direct Customer Support Product and order queries handled directly # Frequently Asked Questions ### What is Swemed SGPT Reagent used for? It is used for quantitative measurement of **ALT / SGPT activity** in professional clinical chemistry laboratories. ### What is the pack size? The pack size is **50 ml**. ### What is included in the pack? - **R1: 40 ml** - **R2: 10 ml** ### What method does Swemed SGPT use? It uses a **Modified IFCC kinetic method**. ### What wavelength is required? The assay uses **340 nm**. ### What temperature is used? The assay is performed at **37°C**. ### What is the sample volume? The macro method uses **100 µl**, while the semi-micro method uses **50 µl**. ### What is the assay factor? The factor is **1746**. ### What is the linearity? The stated linearity is **300 U/L**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the working reagent stable? The manufacturer states **7 days at 15–25°C** and **4 weeks at 2–8°C**. ### Is this product for home testing? No. It is intended for **professional in vitro diagnostic use only**. ### Can it be used on any biochemistry analyser? The laboratory should verify **340 nm capability, kinetic programming, reagent volumes and validated application** before ordering. ### Where can I buy Swemed SGPT Reagent in Bangalore? It can be ordered through **Microsidd India at microsidd.com**, subject to stock availability. # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. ALT / SGPT results should not be interpreted independently to diagnose or exclude liver disease. Clinical interpretation should consider SGOT / AST, bilirubin, alkaline phosphatase, symptoms, patient history and other relevant investigations. The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory SOPs, calibration requirements, quality-control procedures or professional medical judgement. Specimen requirements, reagent preparation, analyser settings, reference intervals, storage, stability and interpretation must always follow current manufacturer instructions and validated laboratory procedures. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 950.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 950.00 INR | In stock | ## Images - Swemed SGPT Reagent 50ml ALT Modified IFCC Method Microsidd 1 - Swemed SGPT Reagent 50ml ALT Modified IFCC Method Microsidd 1 - Swemed SGPT Reagent 50ml ALT Modified IFCC Method Microsidd 1 - Swemed SGPT Reagent 50ml ALT Modified IFCC Method Microsidd 1 - Swemed SGPT Reagent 50ml ALT Modified IFCC Method Microsidd 1 --- > Source: [Microsidd India](https://microsidd.com/products/sgpt-alt-liquid-stable-reagent-kit) > Updated: 2026-09-11