# SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method # Swemed SGOT (AST) Liquid Stable Reagent Kit 50 ml The **Swemed SGOT (AST) Liquid Stable Reagent Kit 50 ml** is a professional clinical biochemistry reagent intended for quantitative determination of **Aspartate Aminotransferase (AST), also known as SGOT**, using the **Modified IFCC kinetic method**. Swemed’s official SGOT literature describes the assay as a kinetic UV method in which AST catalyses transfer of an amino group from L-aspartate to α-ketoglutarate, producing oxaloacetate and L-glutamate. The oxaloacetate then reacts with NADH in the presence of malate dehydrogenase, and the resulting **decrease in absorbance at 340 nm** is proportional to SGOT/AST activity in the sample. SGOT/AST is an important enzyme found in tissues including the **liver, heart, skeletal muscle and kidneys**. Its measurement is commonly included in **liver function testing and broader clinical chemistry enzyme panels**. This product is intended for **professional in vitro diagnostic use only** in pathology laboratories, diagnostic centres, hospitals and clinical chemistry laboratories. * * * ## Product at a Glance Product Detail Information Product Name SGOT / AST Liquid Stable Reagent Kit Brand Swemed Diagnostics Pack Size 50 ml Product Type Clinical Biochemistry Reagent Test Parameter SGOT / AST Full Name Aspartate Aminotransferase Method Modified IFCC Measurement Mode Kinetic UV Reaction Direction Decreasing Wavelength 340 nm Temperature 37°C Specimen Serum Swemed Website Listing Serum / Plasma (EDTA / Heparin) Reagent System R1 + R2 Working Reagent Ratio 4 parts R1 + 1 part R2 Reagent Volume 1000 µl Sample Volume 100 µl Delay Time 60 seconds Reaction Time 180 seconds Reading Interval 60 seconds Calculation Factor 1746 Linearity Up to 300 U/L Storage 2–8°C Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India The technical assay parameters above are taken from Swemed’s official SGOT product insert. * * * # Key Features - **Swemed Diagnostics SGOT / AST Reagent Kit** - Pack size: **50 ml** - **Modified IFCC kinetic method** - UV measurement at **340 nm** - Liquid stable two-reagent system - Suitable for routine AST / SGOT activity measurement - Working reagent prepared using **4 parts R1 + 1 part R2** - Working reagent stability: **7 days at 15–25°C** - Working reagent stability: **4 weeks at 2–8°C** - Linearity up to **300 U/L** - Suitable for professional liver and enzyme-profile testing - For **in vitro diagnostic use only** * * * # Key Benefits ## Modified IFCC Method The Swemed SGOT assay uses a **Modified IFCC kinetic method**, providing a standardised enzymatic approach for routine AST activity measurement. ## Liquid Stable Reagent System The product uses a two-reagent system designed for routine laboratory use and working-reagent preparation. ## Useful Working Reagent Stability The manufacturer states that prepared working reagent remains stable for: - **7 days at 15–25°C** - **4 weeks at 2–8°C** ## Suitable for Routine Liver Function Testing SGOT / AST is commonly measured together with SGPT / ALT, bilirubin, alkaline phosphatase, albumin and total protein as part of **liver function assessment**. ## Professional Laboratory Application The method is suitable for trained laboratory personnel using compatible semi-automated or automated chemistry systems after analyser validation. * * * # Intended Use The **Swemed SGOT / AST Reagent Kit** is intended for quantitative determination of **Aspartate Aminotransferase activity** in professional clinical chemistry laboratories. The official Swemed insert specifies **serum** as the specimen and recommends fresh, clear, non-haemolysed serum. Swemed’s biochemistry listing also associates SGOT with serum/plasma including EDTA and heparin plasma, so the actual specimen type should follow the current kit insert supplied with the product and the laboratory’s validated method. * * * # Test Principle SGOT / AST catalyses the following reaction: **L-Aspartate + α-Ketoglutarate → L-Glutamate + Oxaloacetate** The oxaloacetate formed then reacts with NADH in the presence of **malate dehydrogenase (MDH)**: **Oxaloacetate + NADH + H+ → L-Malate + NAD+** The rate at which NADH is oxidised to NAD+ is measured as a **decrease in absorbance at 340 nm**, and this rate is proportional to AST activity in the sample. * * * # Reagent Composition Component Manufacturer Specification Reagent 1 Tris Buffer pH 7.8 – 6 g/L Reagent 1 L-Aspartate – 30.5 g/L Reagent 1 LDH – 4 kU/L Reagent 1 MDH – 700 U/L Reagent 2 α-Ketoglutarate – 3.4 g/L Reagent 2 NADH – 1.1 g/L The manufacturer also states that the reagents contain **sodium azide 0.95 g/L as preservative**. * * * # Reagent Preparation Prepare the working reagent by mixing: **4 parts Reagent R1 + 1 part Reagent R2** Alternatively, the Swemed insert states that **0.8 ml R1 + 0.2 ml R2** may be used directly instead of 1 ml of pre-mixed working reagent during the assay. The working reagent should have an absorbance **above 1.0 against distilled water at 340 nm**. Reagent falling below this level should not be used. * * * # Working Reagent Stability Storage Condition Stability 15–25°C 7 days 2–8°C 4 weeks * * * # Technical Specifications Test Parameter Manufacturer Specification Parameter SGOT / AST Method Modified IFCC Mode Kinetic Wavelength 340 nm Unit IU/L Temperature 37°C Reaction Slope Decreasing Reagent Volume 1000 µl Sample Volume 100 µl Delay Time 60 seconds Reaction Time 180 seconds Reading Interval 60 seconds Blanking Water Blank Factor 1746 Linearity 300 U/L These values are listed in Swemed’s quick-reference section. * * * # Manual Assay Procedure Component Macro Method Semi-Micro Method Reagent R1 + R2 800 µl + 200 µl 400 µl + 100 µl Sample 100 µl 50 µl Mix well and read the initial absorbance after **1 minute**. Continue readings at approximately **1-minute intervals for 3 minutes**, then calculate the mean absorbance change per minute. ### Calculation **SGOT / AST (U/L) = ΔA/min × 1746** * * * # Measuring Range and Linearity The manufacturer states a linearity of **300 U/L**. When the reaction rate exceeds the stated measuring range, Swemed describes dilution of the sample **1 + 9 with 0.9% NaCl**, followed by multiplication of the obtained result by **10**. * * * # Specimen Requirements The official product insert recommends: - **Fresh serum** - Clear sample - Non-haemolysed specimen This is particularly important because **red blood cells contain AST**, and haemolysis may cause falsely elevated results. * * * # Specimen Stability According to the Swemed product literature, SGOT / AST in serum is reported stable for: Storage Condition Stability 15–30°C 4 days 2–8°C 10 days −20°C 2 weeks * * * # Interference Information The manufacturer reports that under the evaluated conditions: - Bilirubin up to **18 mg/dL** - Haemoglobin up to **300 mg/dL** had negligible effect on the assay. However, haemolysed samples should still be avoided because red cells contain AST and can produce misleadingly elevated results. * * * # Manufacturer Reference Range Group Manufacturer Reference Women < 31 U/L Men < 32 U/L Swemed also advises that each laboratory establish or validate reference intervals appropriate for its own patient population. * * * # Why SGOT / AST Testing Is Important AST is present in several tissues, particularly: - Liver - Cardiac muscle - Skeletal muscle - Kidneys AST activity may rise when cells in these tissues are injured. SGOT / AST testing may therefore form part of laboratory investigations related to: - Liver injury - Hepatitis - Cirrhosis - Muscle injury - Cardiac-related investigations - Acute pancreatitis - Renal disease - Routine liver-function profiles Because AST is **not liver-specific**, results should be interpreted together with other parameters such as ALT / SGPT, bilirubin, alkaline phosphatase, albumin and clinical findings. * * * # SGOT / AST in a Liver Function Profile Test Laboratory Relevance SGOT / AST Hepatic and extra-hepatic enzyme assessment SGPT / ALT More liver-associated transaminase Bilirubin Total Bilirubin metabolism Bilirubin Direct Conjugated bilirubin assessment Alkaline Phosphatase Hepatobiliary and bone-related enzyme Albumin Liver synthetic function / nutritional assessment Total Protein Protein status GGT Hepatobiliary enzyme where included * * * # Storage and Handling Swemed states that the reagents should be: - Stored at **2–8°C** - Protected from light - Protected from contamination - **Not frozen** - Used only within the stated shelf life The manufacturer also advises against using reagent if the initial absorbance at 340 nm falls below the stated acceptable level or if performance requirements are not met. * * * # Safety and Precautions Laboratory personnel should: - Wear suitable gloves - Use eye and face protection - Avoid skin and mucous membrane contact - Avoid ingestion - Follow current Good Laboratory Practice - Handle sodium-azide-containing reagent appropriately - Dispose of reagents according to institutional procedures * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine AST / SGOT testing Diagnostic Centres Liver function testing Hospital Laboratories Clinical chemistry investigations Gastroenterology Centres Liver enzyme assessment Hepatology Centres Liver function monitoring Clinical Chemistry Laboratories Enzyme testing Medical Institutions Diagnostic and teaching laboratories Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted * * * # Useful Tips Before Ordering - Verify that **SGOT / AST** is the required test. - Confirm the pack size: **50 ml**. - Ensure analyser support for **340 nm kinetic measurement**. - Confirm the laboratory uses the **Modified IFCC method**. - Verify compatibility with your analyser. - Confirm suitable sample type. - Avoid haemolysed specimens. - Follow the **4:1 R1:R2 working reagent ratio**. - Store reagents at **2–8°C**. - Do not freeze. - Follow current manufacturer and laboratory quality-control procedures. * * * # Buy Swemed SGOT Reagent in Bangalore Microsidd India supplies **liver-function reagents, clinical biochemistry reagents and laboratory products from Bangalore / Bengaluru, Karnataka**. The **Swemed SGOT / AST Liquid Stable Reagent Kit 50 ml** is suitable for procurement by pathology laboratories, diagnostic centres, hospitals, gastroenterology centres, clinical chemistry laboratories and other professional healthcare buyers. Customers can order through **microsidd.com**, subject to stock availability and delivery serviceability. * * * # Search Relevance This product is relevant for buyers searching for: - SGOT reagent supplier in Bangalore - AST reagent supplier Bengaluru - Swemed SGOT reagent Bangalore - SGOT reagent 50 ml - AST Modified IFCC reagent India - Liver function reagent supplier Bangalore - SGOT kinetic reagent supplier Karnataka - Clinical chemistry reagent supplier near me - Pathology lab reagent supplier Bangalore - SGOT test reagent online India - Swemed Diagnostics reagent supplier India * * * # AI / LLM-Friendly Product Summary ### What is Swemed SGOT Liquid Stable Reagent Kit 50 ml? The **Swemed SGOT / AST Liquid Stable Reagent Kit 50 ml** is a professional clinical chemistry reagent used for quantitative measurement of Aspartate Aminotransferase activity using a **Modified IFCC kinetic UV method**. ### What wavelength is used? The assay is measured at **340 nm**. ### What is the sample volume? The macro method uses **100 µl sample**, while the semi-micro method uses **50 µl**. ### What is the calculation factor? The stated factor is **1746**. ### What is the assay linearity? The manufacturer states a linearity of **300 U/L**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the working reagent stable? Swemed states **7 days at 15–25°C** and **4 weeks at 2–8°C**. ### Where can Swemed SGOT reagent be purchased? It can be purchased from **Microsidd India through microsidd.com**, subject to stock availability. * * * # GEO and Entity Information Entity Details Product SGOT / AST Reagent Kit Brand Swemed Diagnostics Pack Size 50 ml Analyte Aspartate Aminotransferase Common Name SGOT / AST Method Modified IFCC Measurement Kinetic UV Wavelength 340 nm Clinical Category Liver Function / Clinical Chemistry Product Category Laboratory Reagents Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Hospital / Diagnostic Centre / B2B # Why Buy Swemed SGOT Reagent from Microsidd.com? Clinical chemistry reagents should be purchased with careful attention to the **correct analyte, method, reagent configuration, pack size, analyser compatibility, storage requirements, expiry and seller credibility**. Microsidd India has been involved in the **IVD and diagnostic-product sector since 2000** and focuses on laboratory, diagnostic and healthcare supplies. Professional buyers therefore deal with an identifiable diagnostic-products specialist rather than depending only on general marketplace sellers. ## Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic and laboratory focused Seller expertise varies Depends on dealer Product Knowledge Better suited to reagent-specific enquiries Often general marketplace support Dealer dependent Seller Identity Direct purchase from Microsidd India Marketplace and actual seller may differ Usually locally identifiable Method Verification SGOT, Modified IFCC and pack can be verified before order Similar listings may cause confusion Depends on dealer knowledge GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for labs, hospitals and institutions Seller dependent Usually local Product Range Specialist diagnostic and laboratory catalogue Broad but not specialised Usually limited by local inventory Customer Support Direct Microsidd assistance Marketplace plus seller layer Local support Accountability One identifiable specialist supplier Seller may vary between listings Depends on supplier India-Wide Supply Subject to serviceability Broad Usually regional * * * # Benefits of Buying from Microsidd.com Microsidd Advantage Benefit to Laboratory Buyers Diagnostic Product Specialisation Better suited to professional laboratory procurement IVD Experience Since 2000 Long-standing diagnostic-sector experience Direct Seller Accountability Purchase directly from Microsidd India GST Invoice Useful for laboratories and institutional buyers Product Verification Support Method, pack and specifications can be checked before purchase Multi-Category Procurement Liver, renal, lipid, cardiac and other reagents can be sourced together Bangalore-Based Operations Convenient for Bengaluru and Karnataka laboratories India-Wide Supply Delivery to serviceable locations across India Direct Customer Support Product and order queries handled directly by Microsidd * * * # Frequently Asked Questions ### What is Swemed SGOT Reagent used for? It is intended for quantitative determination of **AST / SGOT enzyme activity** in professional clinical chemistry laboratories. ### What is the pack size? The pack size is **50 ml**. ### What method does the Swemed SGOT Reagent use? It uses a **Modified IFCC kinetic method**. ### What wavelength is required? The assay uses **340 nm**. ### What temperature is used? The assay is performed at **37°C**. ### What is the sample volume? The macro method uses **100 µl**, and the semi-micro method uses **50 µl**. ### What is the assay factor? The manufacturer factor is **1746**. ### What is the linearity? The manufacturer states **300 U/L**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the prepared reagent stable? The manufacturer states **7 days at 15–25°C** and **4 weeks at 2–8°C**. ### Can haemolysed serum be used? It should be avoided because red blood cells contain AST and can produce falsely elevated results. ### Is this product for home testing? No. It is intended for **professional in vitro diagnostic use only**. ### Can it be used on any biochemistry analyser? The laboratory should verify **340 nm capability, kinetic programming, reagent ratio, sample volume and validated application** before ordering. ### Where can I buy Swemed SGOT Reagent in Bangalore? It can be ordered through **Microsidd India at microsidd.com**, subject to stock availability. ### Why buy from Microsidd.com? Microsidd India specialises in diagnostic and laboratory products and provides direct seller accountability, GST invoicing for eligible purchases, product-verification support and professional procurement convenience. * * * # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. AST / SGOT is not specific to a single organ. An elevated result should not be used independently to diagnose liver, cardiac, muscular or renal disease. Clinical interpretation should consider ALT / SGPT, bilirubin, alkaline phosphatase, symptoms, history and other relevant investigations. The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement. Specimen requirements, reagent preparation, analyser settings, calibration, reference intervals, storage, stability and interpretation must always follow current manufacturer instructions and validated laboratory procedures. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 950.0 INR - **Vendor:** Microsidd India ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 950.00 INR | In stock | ## Images - SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method - SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method - SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method - SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method - SGOT (AST) Liquid Stable Reagent Kit 50 ml – Modified IFCC Kinetic Method --- > Source: [Microsidd India](https://microsidd.com/products/sgot-ast-liquid-stable-reagent-kit) > Updated: 2026-09-12