# Phosphorus Reagent Kit 2 × 50 ml | UV-Phosphomolybdate Method # Swemed Phosphorus Reagent Kit 2 × 50 ml The **Swemed Phosphorus Reagent Kit 2 × 50 ml** is a professional clinical biochemistry reagent intended for the **quantitative determination of inorganic phosphorus in serum, heparin plasma and urine**. Swemed uses the **UV-Phosphomolybdate method**, in which inorganic phosphate reacts with ammonium molybdate in the presence of sulfuric acid to form non-reduced phosphomolybdate. The amount formed is directly proportional to the inorganic phosphate concentration and is measured by the increase in absorbance at **340 nm**. This product is suitable for professional pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and clinical chemistry facilities using compatible **open-system fully automatic and semi-automatic biochemistry analysers** after method programming and validation. # Product at a Glance Product Detail Information Product Name Phosphorus Reagent Kit Brand [Swemed Diagnostics](https://microsidd.com/collections/swemed-diagnostics "Buy Swemed diagnostics laboratory reagents at microsidd.com") Pack Size **2 × 50 ml** Test Parameter Inorganic Phosphorus / Phosphate Method **UV-Phosphomolybdate** Mode **Endpoint** Wavelength **340 nm** Temperature **37°C** Unit **mg/dL** Standard Concentration **5 mg/dL** Reaction Direction Increasing Reagent Volume **1000 µl** Sample Volume **20 µl** Incubation **10 minutes at room temperature** Linearity **Up to 12 mg/dL** Specimens Serum / Heparin Plasma / Urine Reagent Preparation Ready to Use Storage **18–30°C** Freezing Do Not Freeze Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India Swemed's quick-reference section specifies endpoint mode, 340 nm, 37°C, a 5 mg/dL standard, 1000 µl reagent, 20 µl sample and linearity to 12 mg/dL. # Key Features - **Swemed Diagnostics Phosphorus Reagent Kit** - Pack size: **2 × 50 ml** - **UV-Phosphomolybdate method** - Endpoint photometric assay - Measurement at **340 nm** - Standard concentration: **5 mg/dL** - Reagent volume: **1000 µl** - Sample volume: **20 µl** - Incubation: **10 minutes at room temperature** - Linearity up to **12 mg/dL** - Ready-to-use reagent and standard - Suitable for serum - Suitable for heparin plasma - Suitable for urine - Compatible with open-system semi-auto analysers - Compatible with open-system fully automatic analysers - For **professional in vitro diagnostic use only** # Why Phosphorus Testing Is Important Phosphorus is an essential mineral involved in: - Bone and mineral metabolism - Energy transfer and cellular metabolism - Carbohydrate metabolism - Renal physiology - Acid-base and phosphate balance - Formation of phospholipids and other cellular compounds Swemed notes that phosphorus is mainly associated with calcium in bone, with a smaller portion present as inorganic phosphorus or phosphate esters. The manufacturer literature also notes that abnormal phosphorus levels may be seen in disorders involving parathyroid function, renal failure, bone disease and vitamin D metabolism. Phosphorus is commonly assessed together with: - Calcium - Magnesium - Urea - Creatinine - Sodium - Potassium - Chloride - Other renal and mineral-profile parameters # Test Principle Inorganic phosphate reacts with **ammonium molybdate in the presence of sulfuric acid** to form **non-reduced phosphomolybdate**. The amount of phosphomolybdate formed is directly proportional to the phosphorus concentration in the specimen. The assay is measured by the **increase in absorbance at 340 nm**. # Reagent Preparation Swemed states that: **The reagent and standard are ready to use.** No routine reagent reconstitution is required before performing the assay according to the manufacturer's procedure. # Technical Specifications Parameter Swemed Specification Test Phosphorus Method UV-Phosphomolybdate Mode Endpoint Wavelength **340 nm** Unit **mg/dL** Temperature **37°C** Standard Concentration **5 mg/dL** Reaction Slope Increasing Reagent Volume **1000 µl** Sample Volume **20 µl** Incubation **10 minutes at RT** Blanking Reagent Blank Linearity **12 mg/dL** # Assay Procedure Summary Tube Reagent Standard / Sample Blank 1000 µl — Standard 1000 µl 20 µl Standard Test 1000 µl 20 µl Sample Mix and incubate for: **10 minutes at room temperature** Then read the absorbance of the standard and sample against the reagent blank at: **340 nm**. # Calculation **Phosphorus (mg/dL) = Absorbance of Sample ÷ Absorbance of Standard × Standard Concentration** Manufacturer standard concentration: **5 mg/dL**. # Measuring Range and Linearity Swemed states that the reaction is linear up to: **12 mg/dL** For specimens above this range, the manufacturer recommends dilution **1 + 4 with physiological saline**, repeating the assay and multiplying the obtained result by **5**. # Compatible with Open-System Biochemistry Analysers The **Swemed Phosphorus Reagent Kit** can be used with compatible programmable open-system analysers where the required assay settings can be entered and validated. Open-System Setting Phosphorus Application Mode Endpoint Wavelength **340 nm** Temperature **37°C** Reagent Volume **1000 µl** Sample Volume **20 µl** Standard **5 mg/dL** Incubation **10 minutes at RT** Reaction Increasing Blank Reagent Blank Unit mg/dL Suitable analyser categories include: - **Open-system semi-automatic biochemistry analysers** - **Open-system fully automatic biochemistry analysers** - Programmable clinical chemistry systems supporting 340 nm endpoint photometry The laboratory should verify analyser compatibility and validate the method before routine patient reporting. # Specimen Requirements Swemed lists the following specimen types: - **Serum** - **Heparin plasma** - **Urine** The manufacturer recommends fasting serum specimens where appropriate because meals can lower inorganic phosphate levels. # Important Anticoagulant Guidance Swemed states that: **Heparin is the only acceptable anticoagulant.** The following should be avoided because they may lower or interfere with inorganic phosphate measurement: - EDTA - Fluoride - Citrate # Serum / Plasma Handling Serum and plasma should be separated from erythrocytes as soon as possible. Swemed notes that delayed separation may allow inorganic phosphate and phosphate esters to leak from red cells into the surrounding sample medium. # Urine Specimen Preparation Swemed recommends collecting urine in an **acid-washed, detergent-free container** and acidifying the specimen with hydrochloric acid to **pH below 3**. The insert states that urine should be diluted before testing. For routine assay preparation, Swemed specifies: **Dilute urine 1:10 (1 + 9) with distilled water and multiply the final result by 10.** # Specimen Stability ## Serum / Plasma Storage Condition Stability 15–25°C **1 day** 4–8°C **4 days** −20°C **1 year** ## Urine Storage Condition Stability 15–25°C **2 days** 2–8°C **6 months** # Interfering Substances ## Haemolysis Haemolysis should be avoided because red blood cells contain high levels of inorganic phosphate. ## Anticoagulants The manufacturer states that: - EDTA - Citrate - Fluoride may interfere with the assay. # Manufacturer Reference Ranges Population / Specimen Swemed Reference Range Adults, fasting serum **2.7–4.5 mg/dL** Children under 12 years **4.5–5.5 mg/dL** Children under 1 year **4.5–6.7 mg/dL** Urine, 24 hours **0.3–1.0 g/24 h** Swemed recommends that each laboratory verify appropriate reference intervals for its own population and analyser system. # Storage and Stability Store the reagent: - At **18–30°C** - Protected from light - Protected from contamination - **Do not freeze** - Ensure the labelled shelf life remains valid - Bring reagent and specimens to room temperature before use where required # Safety and Precautions Swemed notes that the reagent contains **sodium azide at 0.95 g/L as preservative**. Professional users should: - Wear suitable gloves - Use eye / face protection - Avoid skin and mucous membrane contact - Never pipette by mouth - Follow Good Laboratory Practice - Dispose of reagents according to institutional procedures # Phosphorus in an Electrolyte & Mineral Profile Phosphorus fits naturally with the existing Swemed electrolyte and mineral reagent range. Parameter Laboratory Role Sodium Extracellular fluid balance Potassium Intracellular / cardiac electrolyte balance Chloride Fluid and acid-base balance Calcium Bone and neuromuscular physiology Magnesium Mineral and metabolic function **Phosphorus** Bone, renal and cellular energy metabolism This reagent is therefore an important component of the **Swemed Electrolyte & Mineral Reagent Combo**. # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine phosphorus estimation Diagnostic Centres Renal and mineral profiles Hospital Laboratories Clinical chemistry Nephrology Centres Renal and phosphate investigations Dialysis Centres Mineral and renal monitoring Endocrinology Laboratories Calcium-phosphorus assessment Clinical Chemistry Laboratories Routine biochemical testing Medical Institutions Teaching and diagnostic use Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply # Useful Tips Before Ordering - Confirm that **Phosphorus – UV-Phosphomolybdate Method** is required. - Verify the pack size: **2 × 50 ml**. - Confirm analyser support for **340 nm endpoint measurement**. - Verify that your analyser is an **open system**. - Confirm sample volume: **20 µl**. - Confirm reagent volume: **1000 µl**. - Verify the standard concentration: **5 mg/dL**. - Use heparin plasma where plasma is required. - Avoid EDTA, citrate and fluoride. - Avoid haemolysed specimens. - Store reagent at **18–30°C**. - Do not freeze. - Perform suitable standardisation and internal quality control before routine use. # Buy Swemed Phosphorus Reagent in Bangalore Microsidd India supplies **electrolyte, mineral-profile, renal-profile and clinical biochemistry reagents from Bangalore / Bengaluru, Karnataka**. The **Swemed Phosphorus Reagent Kit 2 × 50 ml** is suitable for pathology laboratories, diagnostic centres, hospitals, nephrology laboratories, dialysis centres and other professional clinical chemistry facilities. 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The **Swemed Phosphorus Reagent Kit 2 × 50 ml** is a professional clinical chemistry reagent for quantitative measurement of inorganic phosphorus using the **UV-Phosphomolybdate endpoint method**. ### What wavelength is used? **340 nm.** ### What is the sample volume? **20 µl.** ### What is the reagent volume? **1000 µl.** ### What is the standard concentration? **5 mg/dL.** ### What is the incubation time? **10 minutes at room temperature.** ### What is the linearity? **Up to 12 mg/dL.** ### Which specimens can be used? Serum, heparin plasma and urine. ### Which anticoagulants should be avoided? **EDTA, citrate and fluoride** should be avoided because they can interfere with or lower inorganic phosphate measurement. ### Is the reagent ready to use? Yes. Swemed states that the reagent and standard are ready to use. ### Can it be used on semi-automatic analysers? Yes, on compatible **open-system semi-automatic biochemistry analysers** after method programming and validation. ### Can it be used on fully automatic analysers? Yes, on compatible **open-system fully automatic biochemistry analysers** that permit third-party reagent programming and support 340 nm endpoint measurement. # Why Buy Swemed Phosphorus Reagent from Microsidd.com? Clinical chemistry reagents should be purchased only after confirming the **correct parameter, methodology, pack size, analyser wavelength, specimen requirement, storage condition and seller source**. Microsidd India specialises in laboratory and diagnostic products and has long-standing experience in the IVD sector, helping professional buyers source related biochemistry reagents from one focused supplier. Buying Factor Microsidd.com General Online Marketplaces Offline Suppliers Diagnostic Specialisation Laboratory / IVD focused Seller expertise varies Dealer dependent Method Information UV-Phosphomolybdate clearly specified Listing dependent Dealer dependent Pack Verification **2 × 50 ml** clearly identified May vary Stock dependent Open-System Guidance Suitable for technical enquiries Often limited Depends on supplier Related Reagents Calcium, Magnesium, RFT, Electrolytes Multiple unrelated listings Stock dependent GST Invoice Available for eligible orders Seller dependent May vary B2B Procurement Suitable for laboratories Seller dependent Usually suitable Seller Identity Microsidd India Marketplace seller may vary Usually identifiable # Frequently Asked Questions ### What is Swemed Phosphorus Reagent used for? It is used for quantitative determination of **inorganic phosphorus in serum, heparin plasma and urine**. ### What method is used? **UV-Phosphomolybdate Method.** ### What is the pack size? **2 × 50 ml.** ### What wavelength is required? **340 nm.** ### What is the assay mode? **Endpoint.** ### What is the sample volume? **20 µl.** ### What is the reagent volume? **1000 µl.** ### What is the standard concentration? **5 mg/dL.** ### What is the assay linearity? **12 mg/dL.** ### Can urine be tested? Yes. Swemed provides a urine procedure and recommends appropriate acidification and dilution before analysis. ### Can EDTA plasma be used? No. Swemed identifies **heparin as the acceptable anticoagulant** and advises avoiding EDTA, fluoride and citrate. ### How should the reagent be stored? Store at **18–30°C**, protect from light and contamination, and **do not freeze**. ### Is this reagent part of the Swemed Electrolyte & Mineral Combo? Yes. It is the **Phosphorus component** of the Swemed Electrolyte & Mineral Reagent Combo together with Sodium, Potassium, Chloride, Calcium and Magnesium. # Medical and Laboratory Disclaimer The information on this page is provided for **product identification, professional laboratory procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. Phosphorus results should not be interpreted independently to diagnose or exclude renal, parathyroid, metabolic, bone or vitamin D-related disorders. Results should be correlated with calcium, magnesium, renal-function parameters, clinical history and other relevant investigations. For open-system fully automatic and semi-automatic analysers, **instrument compatibility, reagent programming, standardisation, quality control and analytical performance must be verified and validated by the laboratory before routine patient reporting**. The current Swemed package insert supplied with the actual reagent lot should always be treated as the definitive technical specification. # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 950.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 950.00 INR | In stock | ## Images - Swemed Phosphorus Reagent 2x50ml | UV Molybdate Method | Microsidd - Swemed Phosphorus Reagent 2x50ml | UV Molybdate Method | Microsidd - Swemed Phosphorus Reagent 2x50ml | UV Molybdate Method | Microsidd - Swemed Phosphorus Reagent 2x50ml | UV Molybdate Method | Microsidd - Swemed Phosphorus Reagent 2x50ml | UV Molybdate Method | Microsidd --- > Source: [Microsidd India](https://microsidd.com/products/phosphorus-reagent-kit) > Updated: 2026-09-14