# LDH Reagent Kit 25 ml – Modified IFCC / Optimized DGKC Kinetic Method # Swemed LDH Reagent Kit 25 ml The **Swemed LDH Reagent Kit 25 ml** is a professional **clinical biochemistry reagent** intended for quantitative determination of **Lactate Dehydrogenase (LDH) activity** in serum using a kinetic UV method. Swemed’s current catalogue describes LDH under its cardiac reagent range as an **Optimized DGKC method** based on the **pyruvate-to-lactate reaction**, with liquid-stable ready-to-use reagents, high working-reagent stability and a convenient **25 ml pack size**. The current Swemed LDH product literature labels the assay as a **Modified IFCC Method**. Both describe the same pyruvate-to-lactate kinetic LDH principle. The test measures the rate at which **NADH is oxidized to NAD+** during the LDH-catalysed conversion of pyruvate to lactate. The resulting decrease in absorbance is proportional to the LDH activity present in the specimen. This product is intended for **professional in vitro diagnostic use only** in pathology laboratories, hospital laboratories, diagnostic centres and clinical chemistry facilities. * * * ## Product at a Glance Product Detail Information Product Name LDH Reagent Kit Brand Swemed Diagnostics Pack Size 25 ml Reference LDH25 Product Type Clinical Biochemistry Reagent Test Parameter Lactate Dehydrogenase / LDH Method Modified IFCC / Optimized DGKC Kinetic Method Reaction Pyruvate → Lactate Measurement Type UV Kinetic Reaction Direction Decreasing Specimen Serum, Heparin Plasma Wavelength 340 nm Temperature 37°C Reagent Volume 1000 µl Sample Volume 25 µl Delay Time 60 seconds Reaction Time 180 seconds Reading Interval 60 seconds Calculation Factor 8199 Manufacturer Insert Linearity Up to 1000 U/L Storage 2–8°C Working Reagent R1 + R2 Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India The assay parameters above are from the official Swemed LDH product literature. * * * # Key Features - **Swemed Diagnostics LDH Reagent Kit** - Convenient **25 ml pack size** - Modified IFCC / Optimized DGKC kinetic method - Pyruvate-to-lactate reaction - UV kinetic measurement at **340 nm** - Suitable for serum and heparin plasma - Reaction performed at **37°C** - Ready-to-use R1 and R2 components - Working reagent prepared at **4 parts R1 + 1 part R2** - Working reagent stability stated as **1 week at 2–8°C** in the product insert - Suitable for routine clinical chemistry testing - For **professional in vitro diagnostic use only** * * * # Key Benefits ## Kinetic UV Measurement The Swemed LDH assay uses a kinetic UV method in which the decrease in NADH absorbance at **340 nm** is measured over time, providing a direct rate-based estimate of LDH enzyme activity. ## Defined Two-Reagent System The kit uses **R1 and R2**, which can be combined in a **4:1 ratio** to form the working reagent. The manufacturer also permits direct use of **0.8 ml R1 + 0.2 ml R2** during the assay. ## Convenient 25 ml Pack Swemed specifically lists a **25 ml LDH pack** for laboratories with routine or moderate LDH workloads. Its latest catalogue highlights this pack as a convenient size. ## Suitable for Routine Clinical Chemistry LDH measurement is used in a variety of laboratory investigations involving tissue injury and enzyme activity assessment. ## Specialist Laboratory Use The reagent is designed for trained laboratory personnel following validated analyser settings, internal quality control and current manufacturer instructions. * * * # Intended Use The **Swemed LDH Reagent Kit 25 ml** is intended for quantitative determination of **Lactate Dehydrogenase activity** in serum and compatible heparin plasma. The product insert specifically identifies: - Serum - Heparin plasma It also states that **EDTA and sodium citrate should not be used** because they may inhibit the assay. * * * # Test Principle LDH catalyses the reduction of pyruvate to lactate while NADH is oxidized to NAD+: **Pyruvate + NADH + H+ → Lactate + NAD+** The rate of decrease in absorbance caused by NADH oxidation is directly proportional to the amount of LDH activity present in the sample. * * * # Reagent Preparation Swemed instructs laboratories to prepare the working reagent by mixing: **4 parts Reagent R1 + 1 part Reagent R2** The manufacturer states that the prepared working reagent remains stable for **1 week at 2–8°C**. An alternative direct assay setup is: - **R1: 0.8 ml** - **R2: 0.2 ml** The combined reagent should have an absorbance **above 1.0 against distilled water at 340 nm**. If the absorbance is below this level, the reagent should be discarded. * * * # Reagent Composition Component Composition Reagent 1 Tris buffer pH 7.0 – 6 g/L Reagent 1 EDTA – 1.5 g/L Reagent 1 Sodium Pyruvate – 0.2 g/L Reagent 1 L-Alanine – 44.5 g/L Reagent 2 KH₂PO₄ – 0.9 g/L Reagent 2 K₂HPO₄ – 0.8 g/L The reagents also contain sodium azide as a preservative according to the manufacturer insert. * * * # Technical Specifications Test Parameter Manufacturer Specification Parameter LDH Mode Kinetic Wavelength 340 nm Unit IU/L Temperature 37°C Reaction Slope Decreasing Reagent Volume 1000 µl Sample Volume 25 µl Reaction Time 180 seconds Delay Time 60 seconds Delta / Reading Time 60 seconds Blanking Water Blank Factor 8199 Linearity 1000 U/L These quick-reference parameters are provided in the official Swemed LDH insert. * * * # Manual Assay Procedure Component Macro Method Semi-Micro Method Reagent R1 + R2 800 + 200 µl 400 + 100 µl Sample 25 µl 12.5 µl Mix well and measure the initial absorbance after **1 minute**, then repeat readings at approximately **1-minute intervals for 3 minutes**. Calculate the mean absorbance change per minute. ### Calculation **LDH (U/L) = ΔA/min × 8199** * * * # Specimen Requirements Swemed recommends: - Non-haemolysed serum - Heparin plasma Important manufacturer precautions include: - **Do not use haemolysed samples**, because red blood cells contain high concentrations of LDH. - **Do not use EDTA or sodium citrate plasma**, because they may inhibit the assay. - Avoid lipemic samples where possible. * * * # Specimen Stability The Swemed literature states: Specimen Stability Serum Approximately 1–3 days at 2–8°C The manufacturer also notes that **freezing can inactivate liver LDH isoenzymes**, so specimen handling should follow the laboratory’s validated procedure. * * * # Measuring Range and Linearity The official LDH insert states that the method is linear up to **1000 U/L**. For samples exceeding this level, Swemed recommends dilution **1 + 5 with physiological saline**, repeating the assay and multiplying the result by **6**. Swemed’s newer catalogue describes an updated LDH offering with **high linearity up to 1200 IU/L** and working-reagent stability up to 30 days. Because assay configuration may vary by production version, the **current kit label and current package insert supplied with the 25 ml pack should be treated as the controlling specification**. * * * # Manufacturer Reference Range Specimen Manufacturer Reference Serum 230–460 U/L Swemed recommends that each laboratory establish or verify its own reference interval for its patient population. * * * # Why LDH Testing Is Performed Lactate Dehydrogenase is present in many tissues, including: - Liver - Heart - Skeletal muscle - Kidney - Red blood cells LDH may become elevated in several conditions involving tissue damage or cell turnover. The manufacturer literature mentions associations with myocardial injury, pulmonary disease, hepatic disease, haemolytic anaemia, renal disease and muscular dystrophy. LDH testing may therefore form part of laboratory evaluation related to: - Tissue injury - Liver-related investigations - Muscle injury - Haemolysis-related assessment - Renal disease - Selected cardiac investigations - General enzyme-profile testing LDH is **not tissue-specific**, so results should always be interpreted with other laboratory and clinical findings. * * * # LDH in a Clinical Chemistry Profile Related Test Laboratory Relevance LDH General tissue enzyme / tissue injury marker AST / SGOT Liver and muscle enzyme ALT / SGPT Liver-associated enzyme CK-NAC Muscle enzyme assessment CK-MB Cardiac-associated CK fraction Bilirubin Liver / haemolysis-related assessment Creatinine Renal profile Urea Renal profile * * * # Storage and Stability Store the LDH reagents: - At **2–8°C** - Tightly closed - Protected from light - Protected from contamination - **Do not freeze** The reagents should be brought to the required working temperature before testing and should not be used beyond the labelled expiry date. * * * # Safety and Handling The manufacturer notes that the reagents contain **sodium azide**, which requires appropriate laboratory handling. Laboratory personnel should: - Wear suitable gloves - Use eye / face protection - Avoid ingestion or inhalation - Avoid direct skin and mucous-membrane contact - Follow current Good Laboratory Practice - Dispose of reagents according to institutional and applicable safety procedures * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine LDH testing Diagnostic Centres Clinical chemistry enzyme testing Hospital Laboratories Inpatient and outpatient biochemical testing Cardiac Laboratories Selected enzyme-profile testing Liver / Gastroenterology Centres Hepatic enzyme assessment Clinical Chemistry Laboratories Routine LDH measurement Medical Institutions Diagnostic and teaching laboratories Healthcare Organisations Institutional procurement Laboratory Distributors Professional supply where permitted * * * # Useful Tips Before Ordering - Confirm the required method is **LDH Modified IFCC / DGKC kinetic**. - Verify the **25 ml pack size**. - Confirm analyser support for **340 nm kinetic measurement**. - Check that the analyser can maintain **37°C**. - Verify the required sample volume and factor. - Ensure the specimen type is serum or heparin plasma. - Do not use EDTA or citrate plasma for this method. - Avoid haemolysed samples. - Confirm current reagent storage requirements. - Follow the current package insert supplied with the kit. * * * # Buy Swemed LDH Reagent in Bangalore Microsidd India supplies **clinical chemistry, cardiac, liver and biochemistry reagents from Bangalore / Bengaluru, Karnataka** to pathology laboratories, diagnostic centres, hospitals and institutional healthcare buyers. The **Swemed LDH Reagent Kit 25 ml** can be ordered through **microsidd.com**, subject to stock availability and delivery serviceability. * * * # Search Relevance This product is relevant for buyers searching for: - LDH reagent supplier in Bangalore - Swemed LDH Reagent Bangalore - LDH reagent kit 25 ml - Lactate Dehydrogenase reagent Bengaluru - LDH kinetic reagent India - LDH IFCC reagent supplier Bangalore - DGKC LDH reagent Karnataka - Clinical chemistry reagent supplier near me - Pathology lab reagent supplier Bangalore - Cardiac biochemistry reagent supplier India - Buy LDH reagent online India - Swemed Diagnostics reagent supplier India * * * # Product Summary ### What is Swemed LDH Reagent Kit 25 ml? The **Swemed LDH Reagent Kit 25 ml** is a professional clinical biochemistry reagent used for quantitative measurement of Lactate Dehydrogenase activity by a kinetic UV method. ### What method does it use? The current Swemed product literature identifies the assay as a **Modified IFCC kinetic method**, while the newer Swemed catalogue describes LDH as an **Optimized DGKC pyruvate-to-lactate method**. ### What wavelength is used? The assay is measured at **340 nm**. ### What specimen is suitable? The manufacturer lists **serum and heparin plasma**. ### Can EDTA plasma be used? No. Swemed states that **EDTA and sodium citrate may inhibit the assay** and should not be used. ### What is the sample volume? The macro procedure uses **25 µl sample**. ### What is the assay factor? The stated calculation factor is **8199**. ### What is the stated linearity? The current product insert states **1000 U/L**. The latest Swemed catalogue mentions an updated LDH formulation with linearity up to **1200 IU/L**, so laboratories should follow the specification on the current supplied kit. ### Where can Swemed LDH reagent be purchased? It can be purchased from **Microsidd India through microsidd.com**, subject to stock availability. * * * # Entity Information Entity Details Product LDH Reagent Kit Brand Swemed Diagnostics Pack Size 25 ml Analyte Lactate Dehydrogenase Method Modified IFCC / Optimized DGKC Reaction Pyruvate to Lactate Assay Type UV Kinetic Specimen Serum / Heparin Plasma Clinical Category Cardiac / Clinical Chemistry Product Category Laboratory Reagents Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Hospital / Diagnostic Centre / B2B # Why Buy Swemed LDH Reagent from Microsidd.com? LDH and other enzyme reagents require careful procurement because laboratories must verify the **correct method, wavelength, reagent configuration, pack size, storage requirements, analyser compatibility and seller source** before ordering. Microsidd India has been involved in the **IVD and diagnostic-product sector since 2000** and specialises in laboratory, diagnostic and healthcare products. Professional buyers therefore deal with an identifiable specialist seller rather than relying only on an unrelated third-party marketplace listing. ## Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic and laboratory focused Seller expertise varies Depends on individual dealer Product Knowledge Better suited to reagent-specific enquiries Often general marketplace support Dealer dependent Seller Identity Direct purchase from Microsidd India Marketplace and seller may differ Usually identifiable locally Method Verification LDH method and pack can be checked before order Similar listings may cause confusion Depends on supplier knowledge GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for laboratories and institutions Seller dependent Often local Product Range Specialist diagnostic and laboratory catalogue Broad but not specialised Usually limited by local inventory Customer Support Direct Microsidd assistance Marketplace plus seller layer Local support Accountability One identifiable specialist supplier Seller may vary by listing Depends on supplier India-Wide Access Subject to serviceability Broad Usually regional * * * # Benefits of Buying from Microsidd.com Microsidd Advantage Benefit to Laboratory Buyers Diagnostic Product Specialisation Better suited to professional laboratory procurement IVD Experience Since 2000 Long-standing diagnostic-sector experience Direct Seller Accountability Purchase directly from Microsidd India GST Invoice Useful for laboratories and institutions Product Verification Support Method, pack and compatibility can be checked before purchase Multi-Category Procurement Cardiac, liver, renal and other reagents can be sourced together Bangalore-Based Operations Convenient for Bengaluru and Karnataka laboratories India-Wide Supply Delivery to serviceable locations across India Direct Customer Support Product and order-related queries handled directly * * * # Frequently Asked Questions ### What is Swemed LDH Reagent used for? It is intended for quantitative determination of **Lactate Dehydrogenase activity** in professional clinical chemistry laboratories. ### What is the pack size? The pack size is **25 ml**. ### What method is used? The official product insert states **Modified IFCC Method**, while Swemed’s newer catalogue describes an **Optimized DGKC pyruvate-to-lactate method**. ### What wavelength is required? The assay uses **340 nm**. ### What specimen is suitable? Swemed lists **serum and heparin plasma**. ### Can EDTA or citrate plasma be used? No. The manufacturer states that EDTA and sodium citrate may inhibit the assay. ### What is the sample volume? The macro procedure uses **25 µl sample**. ### How is the working reagent prepared? Mix **4 parts R1 with 1 part R2**. ### How long is the working reagent stable? The current product insert states **1 week at 2–8°C**. ### What is the assay factor? The calculation factor is **8199**. ### What is the assay linearity? The current insert states **1000 U/L**. ### Is this suitable for home testing? No. It is intended for **professional in vitro diagnostic use only**. ### Can it be used on any biochemistry analyser? The laboratory should verify **340 nm capability, kinetic programming, reaction temperature, sample volume and validated application** before ordering. ### Where can I buy Swemed LDH Reagent in Bangalore? The product can be ordered from **Microsidd India through microsidd.com**, subject to stock availability. * * * # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. LDH is a non-specific tissue enzyme and an elevated or reduced result must not be used independently to diagnose or exclude any particular disease. Interpretation should consider patient history, clinical findings and other relevant laboratory investigations. The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement. Because Swemed’s current insert and newer catalogue show slightly different performance claims for LDH linearity and working stability, the **current package insert supplied with the actual kit should always be followed as the definitive specification**. * * * # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 850.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 850.00 INR | In stock | ## Images - Swemed LDH Reagent Kit 25ml | Modified IFCC Kinetic Method | Microsidd - Swemed LDH Reagent Kit 25ml | Modified IFCC Kinetic Method Microsidd - LDH Reagent Kit 25 ml – Modified IFCC / Optimized DGKC Kinetic Method - LDH Reagent Kit 25 ml – Modified IFCC / Optimized DGKC Kinetic Method - LDH Reagent Kit 25 ml – Modified IFCC / Optimized DGKC Kinetic Method --- > Source: [Microsidd India](https://microsidd.com/products/ldh-reagent-kit-25-ml-modified-ifcc-optimized-dgkc-kinetic-method) > Updated: 2026-09-11