# HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml # Swemed HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml The **Swemed HDL Cholesterol Reagent Kit** is a professional **direct enzymatic liquid biochemistry reagent** intended for the quantitative determination of **HDL Cholesterol (HDL-C)** in serum and plasma. The assay uses a **two-reagent direct enzymatic method** designed to selectively measure HDL cholesterol without requiring a separate precipitation step. It is intended for use in pathology laboratories, diagnostic centres, hospitals and clinical biochemistry laboratories performing routine lipid profile testing. HDL cholesterol is an important component of lipid-profile assessment. High-density lipoproteins participate in cholesterol transport, and HDL-C measurement is commonly used together with total cholesterol, triglycerides and LDL cholesterol in cardiovascular-risk evaluation. * * * # Product at a Glance Product Detail Information Product Name HDL Cholesterol Reagent Kit Brand Swemed Diagnostics Reagent Type Direct Enzymatic Liquid Test Parameter HDL Cholesterol / HDL-C Assay Format Two-Reagent System Test Type Direct Enzymatic / Photometric Measurement Mode Endpoint Reaction Direction Increasing Specimen Serum, EDTA Plasma, Heparin Plasma Wavelength 630 nm Reaction Temperature 37°C Manual Incubation 10 minutes at room temperature Sample Volume 10 µl Reagent 1 Volume 800 µl Reagent 2 Volume 200 µl Linearity Up to 180 mg/dL Reagent Preparation Ready to Use Calibrator Lyophilized Human Serum Storage 2–8°C Intended Use Professional In Vitro Diagnostic Use Seller Microsidd India Website microsidd.com Location Bangalore / Bengaluru, Karnataka, India * * * # Kit Components The Swemed Direct HDL Cholesterol system consists of: Component Description Reagent 1 Enzyme Reagent Reagent 2 Developer Reagent Calibrator Lyophilized Human Serum The reagents are supplied **ready to use**. The calibrator is reconstituted with the exact volume of deionized water stated on its label, mixed thoroughly and allowed to stand at room temperature for approximately 30 minutes before use. * * * # Key Features - **Swemed Diagnostics HDL Cholesterol Reagent** - Direct enzymatic liquid method - Designed for quantitative **HDL-C determination** - Two-reagent assay format - No separate precipitation step required - Suitable for serum and plasma - Compatible with **serum, EDTA plasma and heparin plasma** - Reagents supplied **ready to use** - Endpoint measurement - Wavelength: **630 nm** - Sample requirement: **10 µl** - Linearity up to **180 mg/dL** - Suitable for routine lipid-profile testing - Professional **in vitro diagnostic use only** - Suitable for pathology labs, hospitals and diagnostic centres * * * # Key Benefits ## Direct HDL Cholesterol Measurement The assay uses selective detergent chemistry and enzymatic reactions to enable **direct determination of HDL cholesterol** without a manual lipoprotein precipitation procedure. ## Ready-to-Use Reagents Reagent 1 and Reagent 2 are supplied ready for laboratory use, helping simplify routine HDL testing workflows. ## Low Sample Volume The assay uses only **10 µl of sample**, making it suitable for routine clinical chemistry workflows where efficient specimen utilisation is important. ## Suitable for Routine Lipid Profiles HDL-C is commonly measured together with: - Total Cholesterol - Triglycerides - LDL Cholesterol - VLDL calculations or related lipid parameters This makes the reagent highly relevant for laboratories performing routine lipid-profile investigations. ## Broad Laboratory Application Suitable for pathology laboratories, hospital laboratories, diagnostic centres and other professional clinical chemistry facilities. * * * # Intended Use The **Swemed HDL Cholesterol Direct Enzymatic Liquid Reagent** is intended for quantitative determination of **HDL cholesterol in serum and plasma**. The product is intended for use by trained laboratory professionals and for **in vitro diagnostic use only**. * * * # Test Principle The method uses a **two-reagent system** based on selective detergent and enzymatic reactions. In the first reagent, non-HDL unesterified cholesterol undergoes an enzyme reaction. The hydrogen peroxide produced during this stage is consumed through a peroxidase reaction, yielding a colourless product. The second reagent contains a detergent that selectively solubilizes HDL, together with cholesterol esterase and a chromogenic coupler. This produces a coloured reaction proportional to the HDL cholesterol concentration present in the specimen. * * * # Technical Specifications Test Parameter Manufacturer Specification Parameter HDL Cholesterol Method Direct Enzymatic Assay Format Two Reagents Mode Endpoint Wavelength 630 nm Reaction Temperature 37°C Reaction Direction Increasing Sample Volume 10 µl Reagent 1 Volume 800 µl Reagent 2 Volume 200 µl Delay 5 seconds Manual Incubation 10 minutes at room temperature Linearity 180 mg/dL * * * # Manual Assay Procedure Addition Blank Calibrator Test HDL Reagent 1 800 µl 800 µl 800 µl HDL Reagent 2 200 µl 200 µl 200 µl Calibrator — 10 µl — Sample — — 10 µl Mix and incubate for **10 minutes at room temperature**, then measure the absorbance of the sample and calibrator against the reagent blank at **630 nm**. * * * # Specimen Requirements Suitable specimens include: - Fresh serum - EDTA plasma - Heparinized plasma Fresh serum is recommended. Serum should be removed from the blood clot as soon as possible. **Citrate-containing anticoagulants should not be used.** * * * # Specimen Stability The manufacturer states that samples are stable for approximately: Storage Condition Stability 2–8°C 7 days * * * # Measuring Range and Linearity The Swemed Direct HDL Cholesterol method is linear up to **180 mg/dL**. Samples with concentrations above this limit may be diluted **1:1**, with the resulting value multiplied by 2 according to the manufacturer procedure. * * * # Reagent Storage and Stability Store the HDL Cholesterol reagents at: **2–8°C** When stored correctly, the reagents remain stable until the expiration date stated on the reagent bottle and kit-box label. * * * # Why HDL Cholesterol Testing Is Important HDL cholesterol is one of the major lipoprotein-related parameters used in clinical lipid assessment. HDL particles participate in the transport of cholesterol in the bloodstream. HDL-C measurement is commonly used as part of cardiovascular risk assessment and lipid-profile interpretation. The Swemed insert describes HDL cholesterol measurement as a useful predictor in coronary heart disease evaluation. HDL-C testing may therefore form part of: - Routine lipid profiles - Cardiovascular-risk assessment - Dyslipidaemia investigation - Metabolic-health evaluation - Diabetes-related lipid monitoring - Preventive health screening - Follow-up of lipid-management programmes Individual results should always be interpreted by qualified healthcare professionals in conjunction with other lipid parameters and the patient's clinical information. * * * # HDL Cholesterol in a Lipid Profile Parameter Laboratory Relevance Total Cholesterol Measures overall circulating cholesterol HDL Cholesterol Measures cholesterol associated with high-density lipoproteins LDL Cholesterol Commonly used in atherogenic-risk evaluation Triglycerides Measures circulating triglyceride concentration VLDL Often estimated as part of lipid-profile interpretation * * * # Who Can Buy This Product? Buyer Type Typical Application Pathology Laboratories Routine HDL-C and lipid-profile testing Diagnostic Centres Cardiovascular and metabolic investigations Hospital Laboratories Clinical chemistry and lipid testing Clinics with Laboratory Facilities Routine biochemical screening Preventive Health Centres Lipid-profile investigations Medical Institutions Laboratory and teaching applications Healthcare Organisations Professional diagnostic procurement Laboratory Distributors Professional supply where permitted Clinical Chemistry Laboratories Routine HDL cholesterol testing * * * # Useful Tips Before Ordering - Verify that **Direct HDL Cholesterol** is the required test parameter. - Confirm compatibility with your biochemistry analyser. - Verify the required **630 nm wavelength** capability. - Confirm that the laboratory workflow supports a two-reagent HDL assay. - Ensure the required calibrator is supplied or available. - Verify storage facilities at **2–8°C**. - Use fresh serum or approved plasma specimens. - Do not use citrate plasma. - Follow the current Swemed assay instructions. - Maintain routine calibration and internal quality control. - Do not use reagent after the stated expiry date. * * * # Buy Swemed HDL Cholesterol Reagent in Bangalore Microsidd India supplies **biochemistry reagents, lipid-profile reagents and clinical laboratory products from Bangalore / Bengaluru, Karnataka**. The Swemed HDL Cholesterol Direct Enzymatic reagent is suitable for procurement by: - Pathology laboratories in Bangalore - Diagnostic centres in Bengaluru - Hospital laboratories - Preventive health centres - Clinical chemistry laboratories - Medical institutions - Professional healthcare buyers Customers outside Bangalore can also order laboratory products through **microsidd.com** for delivery to serviceable locations across India. * * * # Local Search Relevance This product is relevant for buyers searching for: - HDL cholesterol reagent supplier in Bangalore - Direct HDL reagent Bengaluru - Swemed HDL Cholesterol Reagent Bangalore - HDL reagent kit India - HDL direct enzymatic reagent supplier - Lipid profile reagent supplier Bangalore - Clinical chemistry reagent supplier Bengaluru - Biochemistry reagent supplier Karnataka - Pathology lab reagent supplier near me - HDL cholesterol test reagent online India - Swemed Diagnostics reagent supplier India * * * # Product Summary ### What is Swemed HDL Cholesterol Reagent? The **Swemed HDL Cholesterol Direct Enzymatic Liquid Reagent** is a professional two-reagent clinical biochemistry assay for quantitative determination of HDL cholesterol in serum and plasma. ### What method does it use? It uses a **direct enzymatic two-reagent method** involving selective detergent chemistry to measure HDL cholesterol without a separate precipitation step. ### Which specimens can be used? Fresh serum, EDTA plasma and heparinized plasma are acceptable. Citrate-containing anticoagulants should not be used. ### What wavelength is required? The manufacturer specifies **630 nm**. ### What is the sample volume? The assay uses **10 µl of sample**. ### What is the linearity? The stated linearity is **180 mg/dL**. ### Are the reagents ready to use? Yes. Swemed states that the reagents are supplied **ready to use**. ### Where can Swemed HDL Cholesterol reagent be purchased? It can be ordered from **Microsidd India through microsidd.com**, subject to stock availability and delivery serviceability. * * * # GEO and Entity Information Entity Details Product HDL Cholesterol Reagent Brand Swemed Diagnostics Method Direct Enzymatic Assay Format Two-Reagent Liquid Analyte HDL Cholesterol / HDL-C Specimen Serum / EDTA Plasma / Heparin Plasma Category Laboratory Reagents Subcategory Biochemistry Reagents Clinical Category Lipid Profile Seller Microsidd India City Bangalore / Bengaluru State Karnataka Country India Website microsidd.com Buyer Type Laboratory / Healthcare / B2B # Why Buy Swemed HDL Cholesterol Reagent from Microsidd.com? Laboratory reagents require more careful procurement than ordinary consumer products. Buyers should verify the **correct analyte, method, reagent configuration, analyser compatibility, storage requirements, product source, expiry and seller accountability** before placing an order. Microsidd India has been associated with **IVD and diagnostic-product supply since 2000** and focuses on diagnostic, laboratory and healthcare products. This specialist background makes microsidd.com suitable for laboratories that prefer purchasing from an identifiable diagnostic supplier rather than an unrelated marketplace seller. Microsidd also maintains established and authorised manufacturer relationships across multiple diagnostic-product categories, providing professional buyers with a dependable procurement channel for recurring laboratory requirements. * * * # Microsidd.com vs Online Marketplaces vs Offline Suppliers Buying Factor Microsidd.com Large Online Marketplaces Offline / Local Suppliers Seller Specialisation Diagnostic, laboratory and healthcare focused Expertise varies by seller Depends on dealer Laboratory Product Knowledge Product-focused support Often general marketplace support Dealer dependent Seller Identity Direct purchase from Microsidd India Marketplace and actual seller may differ Usually identifiable locally Manufacturer Relationships Established diagnostic manufacturer relationships across multiple categories Depends on seller Varies Product Verification Method, analyte and specifications can be checked before ordering Similar listings may cause confusion Depends on available stock GST Invoice Available for eligible purchases Seller dependent May vary B2B Procurement Suitable for laboratories and institutions Seller dependent Commonly local Product Range Specialist diagnostic and laboratory catalogue Broad but not specialised Usually limited by local inventory Customer Support Direct Microsidd support Marketplace plus seller layer Local support Accountability One identifiable specialist supplier Sellers may vary Depends on supplier Online Convenience Direct ordering through microsidd.com Convenient May require store visit or phone ordering Geographic Reach India-wide subject to serviceability Broad Typically local or regional * * * # Benefits of Buying from Microsidd.com Microsidd Advantage Benefit to Laboratory Buyers Specialist Diagnostic Supplier Better alignment with professional laboratory requirements IVD Experience Since 2000 Long-standing diagnostic-product experience Manufacturer Relationships Established and authorised relationships across diagnostic categories Direct Seller Accountability Purchase directly from Microsidd India GST Invoice Suitable for professional and institutional procurement Product Verification Support Buyers can confirm specifications before ordering Multi-Category Procurement Reagents, diagnostic kits and consumables available together Bangalore-Based Operations Convenient for Bengaluru and Karnataka laboratories India-Wide Supply Useful for laboratories outside Bangalore Direct Customer Support Product and order queries handled by Microsidd * * * # Frequently Asked Questions ### What is Swemed HDL Cholesterol Reagent used for? It is intended for quantitative determination of **HDL cholesterol in serum and plasma** using a direct enzymatic method. ### Is this a direct HDL assay? Yes. The Swemed product uses a **direct enzymatic two-reagent method**. ### Which specimens are acceptable? Fresh serum, EDTA plasma and heparinized plasma are acceptable. ### Can citrate plasma be used? No. The manufacturer states that anticoagulants containing citrate should not be used. ### What wavelength does the assay require? The assay is measured at **630 nm**. ### What is the sample volume? The manufacturer specifies **10 µl**. ### How much reagent is used? The specified automated parameters are **800 µl Reagent 1 and 200 µl Reagent 2**. ### What is the assay linearity? The method is linear up to **180 mg/dL**. ### Are the reagents ready to use? Yes. The reagents are supplied ready to use. ### Does the calibrator need preparation? Yes. The lyophilized calibrator should be reconstituted using the exact volume of deionized water specified on its label and allowed to stand at room temperature for approximately 30 minutes. ### How should the reagent be stored? Store at **2–8°C** according to the manufacturer instructions. ### How long is the sample stable? The manufacturer states sample stability of **7 days at 2–8°C**. ### Is the product intended for home testing? No. It is intended for **professional in vitro diagnostic use only**. ### Can it be used on any biochemistry analyser? The laboratory should verify analyser compatibility, wavelength, reagent programming and validated application before ordering. ### Where can I buy Swemed HDL Cholesterol reagent in Bangalore? The product can be ordered from **Microsidd India through microsidd.com**, subject to availability. ### Can laboratories outside Bangalore buy from Microsidd.com? Yes. Microsidd supplies diagnostic and laboratory products to serviceable locations across India. * * * # Medical and Laboratory Disclaimer The information on this product page is provided for **general product identification, procurement and educational purposes only**. This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**. The information provided by Microsidd India does not replace the official Swemed Diagnostics product insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement. Specimen requirements, methodology, reagent preparation, analyser settings, calibration, reference intervals, storage, stability, quality-control requirements and interpretation should always follow the current manufacturer instructions. Laboratory results must be interpreted by qualified healthcare professionals together with the patient's clinical information and other relevant investigations. * * * # No Return / No Exchange Policy **This product is non-returnable and non-exchangeable.** Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**. For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering. Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law. **An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.** ## Details - **Price:** 1200.0 INR - **Vendor:** Swemed Diagnostics - **Type:** Biochemistry Reagents - **Tags:** Bio Chemistry Reagents, biochemistry ## Variants | Variant | Price | Available | |---------|-------|-----------| | Default Title | 1200.00 INR | In stock | ## Images - HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml - HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml - HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml - HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml - HDL Cholesterol Direct Enzymatic Liquid Reagent Kit 100ml --- > Source: [Microsidd India](https://microsidd.com/products/hdl-cholesterol-direct-reagent-kit) > Updated: 2026-09-11