# CK-MB Reagent Kit 25 ml – Immunoinhibition Kinetic Method

# Swemed CK-MB Reagent Kit 25 ml

The **Swemed CK-MB Reagent Kit 25 ml** is a professional **cardiac biochemistry reagent** intended for measurement of **CK-MB activity in serum** using an **immunoinhibition kinetic method**.

The method measures creatine kinase activity in the presence of an antibody against the CK-M subunit. According to the Swemed product literature, this antibody inhibits CK-MM activity and half of the CK-MB activity while leaving the B-subunit activity available for measurement.

CK-MB is a cardiac-associated enzyme fraction that has historically been used in laboratory assessment of myocardial injury. Modern cardiac evaluation commonly includes additional biomarkers such as cardiac troponins, and CK-MB results should always be interpreted in the context of the complete clinical picture.

This reagent is intended for **professional in vitro diagnostic use only** in pathology laboratories, diagnostic centres, hospitals and clinical biochemistry laboratories.

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# Product at a Glance

Product Detail

Information

Product Name

CK-MB Reagent Kit

Brand

Swemed Diagnostics

Pack Size

25 ml

Product Type

Cardiac Biochemistry Reagent

Test Parameter

CK-MB

Method

Immunoinhibition

Measurement Type

Kinetic

Specimen

Serum

Wavelength

340 nm

Reaction Temperature

37°C

Reaction Slope

Increasing

Cuvette Path Length

1 cm

Blank

Water Blank

Total Reagent Volume

1000 µl

Sample Volume

50 µl

Delay Time

300 seconds

Reaction Time

120 seconds

Reading Interval

60 seconds

Factor

6752

Linearity

Up to 1500 U/L

Units

U/L

Seller

Microsidd India

Website

microsidd.com

Location

Bangalore / Bengaluru, Karnataka, India

The technical assay information above is taken from Swemed Diagnostics' CK-MB product literature.

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# Kit Components

The CK-MB system consists of:

Component

Description

Reagent R1

Ready to Use

Reagent R2

Ready to Use

Swemed states that both reagents are supplied **ready to use** and remain stable until the expiry date stated on the label when stored appropriately.

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# Key Features

-   **Swemed Diagnostics CK-MB Reagent Kit**
-   Convenient **25 ml pack size**
-   Immunoinhibition method
-   Kinetic assay
-   Designed for CK-MB activity measurement
-   Suitable specimen: **serum**
-   Measurement wavelength: **340 nm**
-   Reaction temperature: **37°C**
-   Total reagent volume: **1000 µl**
-   Sample volume: **50 µl**
-   Linearity up to **1500 U/L**
-   Reagents supplied ready to use
-   Suitable for professional cardiac biochemistry testing
-   Intended for pathology laboratories, hospitals and diagnostic centres
-   For **in vitro diagnostic use only**

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# Key Benefits

## Immunoinhibition-Based CK-MB Measurement

The Swemed CK-MB assay uses immunoinhibition to suppress CK-M activity, allowing measurement related to the B-subunit activity of CK-MB.

## Kinetic Measurement

The assay uses a kinetic measurement format with absorbance change monitored over time at **340 nm**.

## Ready-to-Use Reagents

R1 and R2 are supplied ready to use, helping simplify laboratory reagent preparation.

## Convenient 25 ml Pack

The 25 ml configuration is useful for laboratories with routine or moderate CK-MB testing volumes. Swemed's current catalogue also lists CK-MB as a cardiac reagent using an immunoinhibition kinetic method.

## Suitable for Cardiac Biochemistry Workflows

The reagent can be incorporated into professional laboratory cardiac-marker workflows alongside other relevant tests, subject to laboratory protocols and current clinical practice.

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# Intended Use

The **Swemed CK-MB Reagent Kit** is intended for professional laboratory measurement of CK-MB activity in **serum** using an immunoinhibition kinetic method.

It is intended for use by trained laboratory personnel and for **in vitro diagnostic use only**.

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# Test Principle

The assay measures creatine kinase activity in the presence of an antibody directed against the **CK-M monomer**.

According to the manufacturer:

-   CK-MM activity is inhibited.
-   Approximately half of CK-MB activity is inhibited.
-   The B-subunit activity associated with CK-MB remains measurable.
-   CK-BB activity is not inhibited by the antibody.

The resulting kinetic activity is measured photometrically.

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# Reagent Preparation

Swemed instructs laboratories to prepare the working reagent by mixing:

**4 parts Reagent R1 + 1 part Reagent R2**

The combined working reagent is stated to remain stable for approximately **2 weeks at 2–8°C**, protected from direct light.

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# Technical Specifications

Test Parameter

Manufacturer Specification

Parameter

CK-MB

Method

Immunoinhibition

Mode

Kinetic

Wavelength

340 nm

Cuvette

1 cm path length

Reaction Slope

Increasing

Temperature

37°C

Blank

Water Blank

Total Reagent Volume

1000 µl

Sample Volume

50 µl

Delay Time

300 seconds

Reaction Time

120 seconds

Interval Time

60 seconds

Factor

6752

Linearity

1500 U/L

Unit

U/L

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# Manual Assay Procedure

Component

Macro Method

Semi-Micro Method

Reagent R1 + R2

800 + 200 µl

400 + 100 µl

Sample

50 µl

25 µl

After adding the sample, mix and incubate for **300 seconds at 37°C**. Measure the change in absorbance every minute for **2 minutes**, then calculate the mean absorbance change per minute.

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# Specimen Requirements

The manufacturer identifies **serum as the specimen of choice**.

Swemed also notes that CK-MB activity decreases relatively quickly after collection, reporting approximately **10% loss of activity after 1 hour at 25°C**.

Proper sample collection, handling and timely analysis are therefore important.

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# Manufacturer Reference Value

Parameter

Manufacturer Reference

CK-MB

0–24 U/L

Each laboratory should validate reference intervals according to its own population, analyser configuration, method and laboratory policy.

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# Clinical Significance

CK-MB is an isoenzyme of creatine kinase that has historically been associated with cardiac muscle injury.

The Swemed insert states that CK-MB levels may rise within several hours following myocardial infarction, reach a peak later, and subsequently decline when no further myocardial injury occurs.

In current clinical practice, **cardiac troponins are generally the preferred biomarkers for diagnosing acute myocardial injury**, while CK-MB may still have a role in selected laboratory or clinical circumstances.

CK-MB results should never be interpreted in isolation.

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# CK-MB in a Cardiac Laboratory Profile

Test

Laboratory Relevance

CK-MB

Cardiac-associated creatine kinase fraction

Total CK / CK-NAC

Total creatine kinase activity

Troponin I / T

Major biomarkers of myocardial injury

LDH

Tissue injury-related enzyme

AST / SGOT

May be elevated in several tissue-injury conditions

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# Who Can Buy This Product?

Buyer Type

Typical Application

Pathology Laboratories

CK-MB and cardiac biochemistry testing

Diagnostic Centres

Cardiac-marker investigations

Hospital Laboratories

Emergency and inpatient laboratory testing

Cardiac Hospitals

Professional cardiac biochemistry testing

Medical Institutions

Diagnostic and teaching laboratories

Clinical Chemistry Laboratories

Routine enzyme testing

Healthcare Organisations

Institutional laboratory procurement

Laboratory Distributors

Professional supply where permitted

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# Useful Tips Before Ordering

-   Verify that **CK-MB Immunoinhibition** is the required methodology.
-   Confirm the pack size: **25 ml**.
-   Ensure the analyser supports kinetic measurement at **340 nm**.
-   Confirm compatibility with the laboratory's validated analyser application.
-   Verify that testing can be performed at **37°C**.
-   Confirm required reagent and sample volumes.
-   Follow the stated **4:1 R1:R2 working reagent ratio**.
-   Protect prepared working reagent from direct light.
-   Use serum specimens according to laboratory protocol.
-   Analyse specimens promptly because enzyme activity may decline over time.
-   Follow internal quality-control procedures before reporting results.

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# Buy Swemed CK-MB Reagent in Bangalore

Microsidd India supplies **cardiac biochemistry reagents and laboratory diagnostic products from Bangalore / Bengaluru, Karnataka** for professional pathology laboratories, diagnostic centres, hospitals and institutional healthcare buyers.

The **Swemed CK-MB Reagent Kit 25 ml** is suitable for laboratories performing professional clinical chemistry and cardiac-marker testing and can be ordered through **microsidd.com**, subject to stock availability and delivery serviceability.

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# Product Summary

### What is Swemed CK-MB Reagent Kit 25 ml?

The **Swemed CK-MB Reagent Kit 25 ml** is a professional clinical biochemistry reagent for measuring CK-MB activity in serum using an **immunoinhibition kinetic method**.

### What methodology does the Swemed CK-MB reagent use?

It uses an **immunoinhibition method** with kinetic measurement.

### Which specimen is used?

The manufacturer identifies **serum as the sample of choice**.

### What wavelength is required?

The assay is measured at **340 nm**.

### What is the sample volume?

The manufacturer's macro procedure uses **50 µl serum**, while the semi-micro procedure uses **25 µl**.

### What is the linearity?

The product insert states linearity up to **1500 U/L**.

### Are the reagents ready to use?

Yes. Reagent R1 and Reagent R2 are supplied ready to use.

### Where can Swemed CK-MB reagent be purchased?

The product can be purchased from **Microsidd India through microsidd.com**, subject to availability.

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# Entity Information

Entity

Details

Product

CK-MB Reagent Kit

Brand

Swemed Diagnostics

Pack Size

25 ml

Method

Immunoinhibition

Measurement

Kinetic

Parameter

CK-MB

Specimen

Serum

Category

Laboratory Reagents

Subcategory

Biochemistry Reagents

Clinical Category

Cardiac Markers

Seller

Microsidd India

City

Bangalore / Bengaluru

State

Karnataka

Country

India

Website

microsidd.com

Buyer Type

B2B / Laboratory / Healthcare

# Why Buy Swemed CK-MB Reagent from Microsidd.com?

Cardiac and biochemistry reagents require careful procurement because laboratories need the **correct analyte, method, reagent configuration, pack size, analyser compatibility, storage conditions, expiry and dependable seller support**.

Microsidd India has been involved in the **IVD and diagnostic-product sector since 2000** and specialises in diagnostic, laboratory and healthcare supplies rather than operating as a general-purpose ecommerce marketplace.

Microsidd also maintains established manufacturer and authorised brand relationships across several diagnostic categories, giving professional buyers the advantage of purchasing through an identifiable diagnostic-products specialist.

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# Microsidd.com vs Online Marketplaces vs Offline Suppliers

Buying Factor

Microsidd.com

Large Online Marketplaces

Offline / Local Suppliers

Seller Specialisation

Diagnostic and laboratory focused

Seller expertise varies

Depends on dealer

Product Knowledge

Suitable for laboratory-product enquiries

Often general marketplace support

Dealer dependent

Seller Identity

Direct purchase from Microsidd India

Marketplace and actual seller may differ

Usually identifiable

Manufacturer Relationships

Established diagnostic manufacturer relationships across multiple categories

Seller dependent

Varies

Method Verification

Product parameter and methodology can be checked before ordering

Similar listings may cause confusion

Depends on stock and dealer knowledge

GST Invoice

Available for eligible purchases

Seller dependent

May vary

B2B Procurement

Suitable for laboratories and healthcare institutions

Seller dependent

Usually local

Product Range

Specialist laboratory and diagnostic catalogue

Broad but not specialised

Usually limited by local stock

Customer Support

Direct Microsidd support

Marketplace plus seller layer

Local support

Seller Accountability

One identifiable specialist supplier

Seller may vary by listing

Depends on supplier

Online Convenience

Direct ordering through microsidd.com

Convenient

May require phone or store visit

Geographic Reach

India-wide subject to serviceability

Broad

Usually regional

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# Benefits of Buying from Microsidd.com

Microsidd Advantage

Benefit to Laboratory Buyers

Diagnostic Product Specialisation

Better suited to professional laboratory procurement

IVD Experience Since 2000

Long-standing experience in diagnostic-product supply

Manufacturer Relationships

Established relationships across diagnostic categories

Direct Seller Accountability

Purchase directly from Microsidd India

GST Invoice

Useful for laboratories and institutional buyers

Product Verification Support

Method, pack and product specifications can be confirmed before ordering

Multi-Category Procurement

Cardiac, biochemistry, rapid-test and laboratory products available together

Bangalore-Based Operations

Convenient for laboratories in Bengaluru and Karnataka

India-Wide Supply

Delivery to serviceable locations across India

Direct Customer Support

Product and order queries handled directly by Microsidd

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# Frequently Asked Questions

### What is Swemed CK-MB Reagent used for?

It is intended for professional measurement of **CK-MB activity in serum** using an immunoinhibition kinetic method.

### What is the pack size?

The listed pack size is **25 ml**.

### What method does the Swemed CK-MB Kit use?

The assay uses an **immunoinhibition kinetic method**.

### Which specimen is recommended?

Swemed identifies **serum as the sample of choice**.

### What wavelength is used?

The assay uses **340 nm**.

### What is the reaction temperature?

The assay is performed at **37°C**.

### What is the total reagent volume?

The stated reagent volume is **1000 µl**.

### What is the sample volume?

The macro procedure uses **50 µl** and the semi-micro procedure uses **25 µl**.

### What is the assay linearity?

The manufacturer insert states **1500 U/L**.

### How are R1 and R2 prepared?

Mix **4 parts R1 with 1 part R2**.

### How long is the combined reagent stable?

The manufacturer states approximately **2 weeks at 2–8°C**, protected from direct light.

### Is CK-MB a cardiac marker?

CK-MB is a cardiac-associated creatine kinase isoenzyme historically used in the laboratory evaluation of myocardial injury. Current interpretation should follow contemporary clinical practice and should not rely on CK-MB alone.

### Is the reagent suitable for home testing?

No. It is intended for **professional in vitro diagnostic use only**.

### Can this reagent be used on any biochemistry analyser?

The laboratory should verify analyser compatibility, wavelength, kinetic programming, reaction temperature and validated method before ordering.

### Where can I buy Swemed CK-MB Reagent in Bangalore?

The product can be ordered from **Microsidd India through microsidd.com**, subject to stock availability.

### Can laboratories outside Bangalore purchase it?

Yes. Microsidd supplies diagnostic and laboratory products to serviceable locations across India.

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# Medical and Laboratory Disclaimer

The information on this product page is provided for **general product identification, procurement and educational purposes only**.

This product is intended for **professional in vitro diagnostic use by trained laboratory personnel**.

CK-MB is a laboratory biomarker and should not be used alone to diagnose or exclude myocardial infarction or other cardiac conditions. Current clinical evaluation of suspected myocardial injury may require cardiac troponins, ECG findings, symptoms and other clinical investigations.

The information provided by Microsidd India does not replace the official Swemed Diagnostics package insert, analyser instructions, laboratory standard operating procedures, calibration requirements, quality-control procedures or professional medical judgement.

Specimen requirements, methodology, reagent preparation, analyser settings, reference intervals, storage, stability, quality-control requirements and interpretation must always follow the current manufacturer instructions and validated laboratory procedures.

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# No Return / No Exchange Policy

**This product is non-returnable and non-exchangeable.**

Please verify the **brand, model, compatibility, pack size and product specifications before placing your order**.

For laboratory reagents, customers should specifically verify the **test parameter, methodology, analyser compatibility, reagent configuration and pack size** before ordering.

Genuine cases involving an **incorrect product received** will be handled separately according to Microsidd.com's applicable policies and applicable law.

**An original, continuous, unedited and uncut unboxing video is compulsory for making any claim regarding an incorrect product received.**

## Details

- **Price:** 3499.0 INR
- **Vendor:** Swemed Diagnostics
- **Type:** Biochemistry Reagents
- **Tags:** biochemistry

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| Default Title | 3499.00 INR | In stock |

## Images

- Swemed CK-MB Reagent Kit 25ml | Immunoinhibition Method | Microsidd
- Swemed CK-MB Reagent Kit 25ml | Immunoinhibition Method | Microsidd
- Swemed CK-MB Reagent Kit 25ml | Immunoinhibition Method | Microsidd
- Swemed CK-MB Reagent Kit 25ml | Immunoinhibition Method | Microsidd
- Swemed CK-MB Reagent Kit 25ml | Immunoinhibition Method | Microsidd

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> Source: [Microsidd India](https://microsidd.com/products/ck-mb-reagent-kit-25-ml)
> Updated: 2026-09-11
