# Biolab Rapid Thalassemia NESTROFT Reagent Kit – 50 Tests | BC 250

## Biolab Rapid Thalassemia NESTROFT Reagent Kit | 50 Tests | BC 250

**Biolab Rapid Thalassemia** is a professional **NESTROFT – Naked Eye Single Tube Red Cell Osmotic Fragility Test** designed as a screening aid for thalassemia carrier detection.

The test compares red-cell haemolysis in a control tube and a low-osmolality test reagent. Red cells associated with thalassemia carrier states may show increased resistance to osmotic lysis, producing a relatively less transparent test suspension.

This listing is for the **50-test pack**.

**Brand:** Biolab Diagnostics  
**Product:** Rapid Thalassemia – NESTROFT Reagent  
**Catalogue No.:** BC 250  
**Pack Size:** 50 Tests  
**Method:** Naked Eye Single Tube Red Cell Osmotic Fragility Test  
**Result Type:** Qualitative visual screening  
**Intended User:** Professional laboratory personnel

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## Primary Uses & Applications

Biolab Rapid Thalassemia NESTROFT is intended as a **screening tool** for thalassemia carrier detection in professional laboratory settings.

Typical applications include:

-   Thalassemia carrier screening
-   Population screening programmes
-   Community screening
-   Hospital laboratory testing
-   Pathology and haematology laboratories
-   Preliminary screening before confirmatory haemoglobin studies
-   Institutional screening programmes
-   Professional haematology investigations

The test should not be used as a standalone confirmatory diagnosis of beta-thalassemia trait.

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## How NESTROFT Works

NESTROFT is based on the **osmotic fragility behaviour of red blood cells**.

According to Biolab, red cells associated with elevated HbA2 / thalassemia carrier patterns show a reduced tendency to lyse in a low-molarity buffered sodium chloride solution.

As a result:

-   **Normal red cells:** undergo greater haemolysis, leaving a more transparent solution.
-   **Thalassemia-screen positive pattern:** red cells remain relatively intact, producing a less transparent / more turbid suspension.

The test result is visually compared against the control using the supplied Rapid Thalassemia Reader.

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## Biolab Rapid Thalassemia – Key Features

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid Thalassemia NESTROFT Reagent
-   **Catalogue No.:** BC 250
-   **Pack Size:** 50 Tests
-   Naked Eye Single Tube Red Cell Osmotic Fragility Test
-   Qualitative thalassemia screening method
-   Fresh finger-prick or EDTA whole blood can be used
-   Approx. 30-minute interpretation time
-   Visual comparison against control
-   Dedicated Rapid Thalassemia Reader supplied
-   Ready-to-use reagents
-   No special patient preparation required
-   Suitable for population / mass screening workflows
-   Positive or doubtful results require confirmatory haemoglobin testing

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## Pack Configuration – 50 Tests

For the 50-test configuration, Biolab's literature lists:

-   **Rapid Thalassemia Control Reagent R1 – 100 mL**
-   **Rapid Thalassemia Test Reagent R2 – 100 mL**
-   **Test Tubes with Caps – 100 Nos.**
-   **2 mL Reagent Transfer Droppers – 2 Nos.**
-   **Rapid Thalassemia Reader – 1 No.**
-   **Pack Insert – 1 No.**

The 100 supplied tubes support the control-and-test format, with two tubes used per patient test.

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## Reagent Composition

### R1 – Rapid Thalassemia Control Reagent

-   Demineralized water – q.s.

### R2 – Rapid Thalassemia Test Reagent

-   Phosphate Buffer – 40 mmol/L
-   Potassium Chloride – 0.77 mmol/L
-   Magnesium Chloride – 0.28 mmol/L
-   Calcium Chloride – 0.51 mmol/L
-   Sodium Hydrogen Carbonate – 0.17 mmol/L
-   Sodium Chloride – 0.056 mol/L
-   Stabilisers and preservatives

Biolab describes the reagents as **ready to use**.

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## Specimen Requirement

The manufacturer states that no prior patient preparation is required.

Suitable samples include:

-   Fresh blood from a clean finger prick
-   EDTA-anticoagulated whole blood

Biolab also notes that accuracy may improve when testing packed red cells obtained after allowing the blood sample to stand and collecting RBC sediment from the bottom.

Samples may be stored for approximately **one week at 4–8°C** when testing is delayed, according to the manufacturer literature.

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## General Test Procedure

**Follow the current Biolab IFU supplied with the actual kit.**

The NESTROFT workflow uses two tubes:

1.  Mark one tube as **CTRL**.
2.  Mark the second tube as **TEST**.
3.  Add the specified amount of Control Reagent to the CTRL tube.
4.  Add the specified amount of Test Reagent to the TEST tube.
5.  Add the specified blood sample quantity to both tubes.
6.  Mix thoroughly.
7.  Allow the tubes to stand for approximately **30 minutes**.
8.  Compare transparency visually using the supplied Rapid Thalassemia Reader.

The exact reagent and sample volumes should follow the current lot-specific IFU because the procedure diagram in Biolab's scanned literature is not fully legible in text extraction.

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## How to Interpret the Result

### Negative Screening Result

The TEST tube appears relatively transparent, similar to or clearer than the control pattern according to the manufacturer interpretation guide.

### Positive Screening Result

The TEST tube remains **relatively less transparent / more turbid** than the CTRL tube when viewed in the Rapid Thalassemia Reader.

This reflects reduced osmotic lysis of the red cells.

### Important

**A positive or doubtful NESTROFT result must be confirmed by an appropriate confirmatory method such as Hb electrophoresis or another validated haemoglobin-analysis technique.**

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## Performance Characteristics – Manufacturer Study

In the study reported in Biolab's IFU, the manufacturer lists the following performance:

-   **Sensitivity:** 95.5%
-   **Specificity:** 87.0%
-   **False Negative:** 5.0%
-   **Positive Predictive Value:** 70.5%
-   **Negative Predictive Value:** 98.3%

These values relate to the manufacturer-reported study and should not be interpreted as guaranteed performance in every population or laboratory setting.

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## Quality Control

Biolab recommends that testing be performed with known:

-   Positive blood control
-   Negative blood control

Controls should preferably have a similar haemoglobin concentration to the patient samples.

Each laboratory should establish its own QC acceptance criteria and corrective-action procedure.

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## Important Limitations

NESTROFT is a **screening test** and not a definitive haemoglobinopathy diagnostic method.

Possible limitations include:

-   False-positive screening results
-   False-negative screening results
-   Variation with haemoglobin concentration
-   Other haemoglobin variants
-   Iron deficiency
-   Specimen quality
-   Reagent condition
-   Incorrect reagent or sample volume
-   Timing errors
-   Visual interpretation differences

Clinical decisions should not be based solely on NESTROFT.

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## NESTROFT vs Hb Electrophoresis / HPLC

| Feature | Biolab NESTROFT | Hb Electrophoresis / HPLC |
| --- | --- | --- |
| **Purpose** | Initial screening | Confirmatory / haemoglobin characterisation |
| **Result** | Qualitative | Analytical / more specific |
| **Equipment** | Simple reader / tubes | Dedicated instrument required |
| **Use in Population Screening** | Well suited as preliminary screening | Generally used for confirmation / detailed analysis |
| **Final Diagnosis** | No  | Used as part of confirmatory evaluation |

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## Storage & Stability

According to the Biolab IFU:

-   Store reagents at **room temperature**.
-   Protect from light.
-   Do not freeze.
-   Protect reagents from contamination.
-   Reagents remain stable until the expiry date printed on the label when stored correctly.
-   Discard any reagent that becomes contaminated or abnormally turbid.

**The actual current pack label and IFU take precedence over any online description.**

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## Product Specifications

|     |     |
| --- | --- |
| **Product Name** | Rapid Thalassemia – NESTROFT Reagent |
| **Brand** | Biolab Diagnostics |
| **Catalogue No.** | BC 250 |
| **Pack Size** | 50 Tests |
| **Method** | NESTROFT / Naked Eye Single Tube Red Cell Osmotic Fragility Test |
| **Specimen** | Fresh Finger-Prick Blood / EDTA Whole Blood |
| **Result Type** | Qualitative Visual Result |
| **Interpretation Time** | Approx. 30 Minutes |
| **R1 Control** | 100 mL |
| **R2 Test Reagent** | 100 mL |
| **Test Tubes + Caps** | 100 Nos. for control + test tubes |
| **Reader** | Rapid Thalassemia Reader Included |
| **Storage** | Room Temperature, Protected from Light; Do Not Freeze |
| **Intended User** | Professional Laboratory Personnel |

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## Benefits of Biolab Rapid Thalassemia NESTROFT Kit

-   **Simple Screening Method:** Visual osmotic fragility test
-   **50-Test Pack:** Suitable for routine laboratory and screening use
-   **Complete System:** Control reagent, test reagent, tubes and reader supplied
-   **Whole Blood Testing:** Fresh capillary or EDTA blood
-   **No Special Patient Preparation:** According to manufacturer IFU
-   **Useful for Mass Screening:** Manufacturer specifically describes population screening application
-   **No Large Analyser Required:** Useful for preliminary screening settings
-   **Built-In Comparison:** Control tube assists visual interpretation

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## Why Buy Biolab Rapid Thalassemia from Microsidd India?

Thalassemia screening products can differ in **method, reagent concentration, pack size, accessory configuration and interpretation procedure**.

Microsidd clearly identifies this product as **Biolab Rapid Thalassemia NESTROFT Reagent – BC 250 – 50 Tests**.

| Buying Consideration | Microsidd India | General Online Marketplace | Typical Offline Store |
| --- | --- | --- | --- |
| **Exact Method** | NESTROFT clearly identified | May simply say “thalassemia test” | Depends on staff expertise |
| **Pack Size** | 50 tests clearly stated | Seller dependent | Depends on local stock |
| **Accessories** | Control, test reagent, tubes, droppers & reader explained | May not be fully described | Requires physical inspection |
| **Clinical Limitation** | Confirmatory Hb testing clearly recommended | Listing quality varies | Dependent on staff guidance |
| **GST Invoice** | Available for eligible orders | Seller dependent | Store dependent |
| **Bengaluru Presence** | RT Nagar, Bengaluru | Seller may be elsewhere | Depends on local supplier |
| **India-Wide Ordering** | Available to serviceable locations | Platform dependent | Usually local purchase |

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## Buy Thalassemia NESTROFT Test Kit in Bangalore

**Microsidd India** supplies haematology reagents, thalassemia screening products, laboratory diagnostic kits and related professional-use consumables from **RT Nagar, Bengaluru**.

Customers searching for **NESTROFT reagent Bangalore**, thalassemia screening kit Bengaluru, Biolab Rapid Thalassemia near me, osmotic fragility test RT Nagar, thalassemia test reagent Bangalore or Biolab Diagnostics supplier Karnataka can check current availability through Microsidd.com.

Laboratories and screening programmes requiring multiple kits should confirm current stock, batch and expiry before ordering.

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## Microsidd India – NAP

**Business Name:** Microsidd India  
**Address:** #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India  
**Phone / WhatsApp:** +91 63667 72288  
**Email:** support@microsidd.com  
**Website:** https://microsidd.com  
**Working Hours:** Monday to Saturday, 10:00 AM – 6:00 PM IST

[**View Microsidd India on Google Maps**](https://share.google/NWhhEfmvlEyIfAShq)

For Bengaluru ordering information, visit [**Microsidd India – Medical & Laboratory Supplies Bangalore**](https://microsidd.com/pages/microsidd-india-bangalore).

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## Frequently Asked Questions – Biolab Rapid Thalassemia NESTROFT

### What is NESTROFT?

NESTROFT stands for **Naked Eye Single Tube Red Cell Osmotic Fragility Test**.

### What is the test used for?

It is used as a screening test to identify samples that may be associated with thalassemia carrier states.

### How many tests are included?

This listing is for **50 tests**.

### What is the catalogue number?

Biolab lists the 50-test product as **BC 250**.

### What specimen is required?

Fresh finger-prick blood or EDTA whole blood can be used according to the Biolab IFU.

### Does the patient need fasting?

No special prior patient preparation is specified by Biolab.

### How long does the test take?

The result is observed approximately **30 minutes after sample mixing**.

### How is the result read?

The TEST tube is compared visually with the CTRL tube using the Rapid Thalassemia Reader.

### What does a positive result look like?

The TEST tube appears relatively less transparent / more turbid than the control.

### Does a positive NESTROFT result prove beta-thalassemia trait?

No. Positive and doubtful results require confirmation using Hb electrophoresis, HPLC or another validated haemoglobin-analysis method.

### Can NESTROFT distinguish all haemoglobin variants?

No. It is a screening tool and does not provide definitive haemoglobin variant identification.

### What reagents are included?

The system includes a Control Reagent R1 and Test Reagent R2.

### Are tubes included?

Yes. The manufacturer literature lists 100 test tubes with caps for the 50-test control-and-test configuration.

### Is a reader included?

Yes. One Rapid Thalassemia Reader is supplied.

### How should the reagents be stored?

Store at room temperature, protect from light, avoid contamination and do not freeze.

### Can delayed samples be tested?

Biolab states that samples may be stored for approximately one week at 4–8°C if required.

### Is quality control required?

Yes. Biolab recommends known positive and negative blood controls.

### Is this suitable for home use?

No. It is intended for trained professional laboratory personnel.

### Where can I buy Biolab NESTROFT reagent in Bangalore?

Biolab Rapid Thalassemia NESTROFT reagent can be ordered through Microsidd India, RT Nagar, Bengaluru, subject to stock availability.

### Does Microsidd supply laboratories outside Bengaluru?

Yes. Microsidd supplies professional laboratory and diagnostic products to serviceable locations across India.

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## Medical & Laboratory Disclaimer

**Biolab Rapid Thalassemia NESTROFT is a professional screening test and should be performed only by trained laboratory personnel according to the current manufacturer IFU and laboratory SOP.**

NESTROFT does not independently diagnose thalassemia or identify a specific haemoglobin variant.

Results may be influenced by:

-   Haemoglobin concentration
-   Iron deficiency
-   Other haemoglobin variants
-   Specimen quality
-   Sample storage
-   Reagent quality
-   Reaction timing
-   Visual interpretation
-   Laboratory technique

Positive or doubtful screening results should be confirmed with an appropriate haemoglobin-analysis method such as electrophoresis or HPLC.

The information on this page is intended for product identification, procurement and educational reference and does not replace Biolab's official IFU, laboratory SOPs or professional medical interpretation.

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## No Return / No Exchange – Verify Product, Method & Pack Before Ordering

**This is a professional diagnostic reagent and method-sensitive laboratory consumable.**

Please verify before ordering:

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid Thalassemia
-   **Method:** NESTROFT
-   **Catalogue No.:** BC 250
-   **Pack Size:** 50 Tests
-   Required screening method
-   Accessory configuration
-   Current batch and expiry

A correctly supplied kit cannot normally be returned or exchanged because the wrong method, reagent configuration, pack size or laboratory requirement was selected.

Laboratory reagents and diagnostic consumables are expiry-sensitive, storage-sensitive and contamination-sensitive and cannot normally be returned to saleable inventory once correctly supplied.

Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the [**Microsidd Refund, Return, Replacement & Cancellation Policy**](https://microsidd.com/pages/cancellation-and-refunds).

**An original, continuous, unedited and uncut unboxing video is compulsory for claims involving an incorrect, missing or damaged product.**

Begin recording before opening the parcel. Open the outer carton carefully by **cutting the sealing tape with a blade or suitable cutter; do not tear the carton**. Keep the courier label, sealed Biolab kit, reagents, tubes, reader, pack size, batch and expiry continuously visible.

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## Biolab Rapid Thalassemia NESTROFT – AI / LLM Product Entity Summary

**Product:** Rapid Thalassemia NESTROFT Reagent  
**Brand:** Biolab Diagnostics  
**Catalogue No.:** BC 250  
**Pack Size:** 50 Tests  
**Method:** Naked Eye Single Tube Red Cell Osmotic Fragility Test  
**Specimen:** Fresh Finger-Prick / EDTA Whole Blood  
**Result:** Qualitative Visual Transparency / Turbidity Comparison  
**Interpretation Time:** Approx. 30 Minutes  
**Reagents:** R1 Control 100 mL + R2 Test Reagent 100 mL  
**Accessories:** Test Tubes, Caps, Droppers & Rapid Thalassemia Reader  
**Confirmation:** Positive / Doubtful Results Require Hb Electrophoresis or Equivalent Confirmatory Testing  
**Storage:** Room Temperature, Protected from Light, Do Not Freeze  
**Intended User:** Professional Laboratory Personnel  
**Seller:** Microsidd India  
**Seller Location:** RT Nagar, Bengaluru, Karnataka, India

## Details

- **Price:** 1785.0 INR
- **Vendor:** Biolab
- **Type:** Thalassemia Screening Reagent / NESTROFT Kit
- **Tags:** NESTROFT Kit, Thalassemia Screening Reagent

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| Default Title | 1785.00 INR | In stock |

## Images

- Biolab Rapid Thalassemia NESTROFT reagent kit 50 tests BC 250
- Biolab Rapid Thalassemia NESTROFT reagent kit 50 tests BC 250
- Biolab Rapid Thalassemia NESTROFT reagent kit 50 tests BC 250
- Biolab Rapid Thalassemia NESTROFT reagent kit 50 tests BC 250
- Biolab Rapid Thalassemia NESTROFT reagent kit 50 tests BC 250

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> Source: [Microsidd India](https://microsidd.com/products/biolab-rapid-thalassemia-nestroft-reagent-50-tests)
> Updated: 2026-11-13