# Biolab Rapid Malaria Stain Kit JSB I & II 2×500 mL | One-Minute Blood Parasite Stain

## Biolab Rapid Malaria Stain Kit JSB I & II | 2 × 500 mL | One-Minute Blood Parasite Stain

**Biolab Rapid Malaria Stain Kit (JSB I & II)** is a professional laboratory staining system designed for rapid staining of peripheral blood smears for microscopic demonstration of **malaria parasites and blood-cell morphology**.

The system uses **polychromated Methylene Blue and Eosin** to differentially stain basophilic and acidophilic cellular components, helping laboratory professionals visualise blood cells and haemoparasites during microscopy.

The principal stain pack contains:

-   **Rapid-Malaria JSB I – 500 mL**
-   **Rapid-Malaria JSB II – 500 mL**

The Biolab manufacturer insert also lists an **Easyfix Peripheral Smear Fixative – 50 mL** within the staining system. Verify the exact contents of the current supplied pack before ordering if the fixative is specifically required.

**Brand:** Biolab Diagnostics  
**Product:** Rapid Malaria Stain Kit – JSB I & II  
**Pack Size:** 2 × 500 mL main stain reagents  
**Method:** Rapid JSB Blood Parasite Staining  
**Application:** Malaria Parasite Microscopy  
**Intended User:** Professional Laboratory Personnel

* * *

## Primary Uses & Applications

Biolab Rapid Malaria Stain is primarily designed for staining peripheral blood smears for microscopic examination of malaria parasites.

Professional applications include:

-   Malaria parasite screening by microscopy
-   Peripheral blood smear examination
-   Thin-smear staining
-   Thick-smear staining
-   Haemoparasite microscopy
-   Clinical pathology laboratories
-   Haematology laboratories
-   Hospital diagnostic laboratories
-   Malaria screening programmes
-   Teaching laboratories and medical colleges
-   Professional parasitology workflows

The manufacturer insert specifically describes microscopic appearance for malaria parasites including _Plasmodium vivax_ trophozoites and _Plasmodium falciparum_ trophozoites / gametocytes.

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## How Biolab JSB Malaria Stain Works

The staining principle is based on the differential affinity of **polychromated Methylene Blue and Eosin** for cellular structures.

Biolab states that the stains react specifically with:

-   Basophilic cellular components
-   Acidophilic cellular components
-   Blood cells
-   Haemoparasites

This produces colour contrast that allows malaria parasites and blood-cell structures to be visualised microscopically.

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## Rapid Malaria Stain – Key Features

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid Malaria Stain JSB I & II
-   **Pack:** 2 × 500 mL
-   Rapid blood-parasite staining system
-   Designed for malaria parasite microscopy
-   Suitable for thin peripheral blood smears
-   Suitable for thick peripheral blood smears
-   Ready-to-use reagents
-   No working-reagent preparation required
-   Uses Methylene Blue / Eosin-based differential staining
-   Compatible with manual slide staining
-   Suitable for Coplin-jar / wide-mouth bottle workflows
-   Can also be used in bulk slide-staining workflows according to the IFU
-   Microscopic examination performed under oil immersion
-   Long manufacturer-stated reagent stability when stored correctly

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## Kit Composition

### Rapid-Malaria JSB I – 500 mL

The manufacturer insert lists:

-   Methylene Blue – 4.3 mmol/L
-   Sulphuric Acid – 1%
-   Potassium Dichromate
-   Phosphate Buffer
-   Polychromating materials
-   Preservatives
-   Stabilisers

### Rapid-Malaria JSB II – 500 mL

The manufacturer insert lists:

-   Eosin – 4.3 mmol/L
-   Phosphate-based buffer system
-   Preservatives
-   Stabilisers

### Easyfix Peripheral Smear Fixative

The Biolab insert also lists **Easyfix Peripheral Smear Fixative – 50 mL**.

Because commercial pack configurations can change, laboratories requiring the fixative should verify the current sealed-pack contents before ordering.

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## Ready-to-Use Reagents

Biolab specifies that the reagents are **ready to use**.

No dilution or working-reagent preparation is described for the JSB I and JSB II stains.

This simplifies routine laboratory staining and helps reduce reagent-preparation variability.

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## Sample / Specimen

The manufacturer insert describes the use of **peripheral blood samples prepared on clean glass slides**.

Blood samples may be obtained during febrile episodes when malaria is clinically suspected.

Biolab also states that **EDTA-anticoagulated blood may be used** without substantial change in staining results.

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## Thin Blood Smear Application

A thin peripheral blood film may be prepared by spreading a small drop of blood with a fine-edged spreader.

The smear should be:

-   Air dried
-   Fixed using the appropriate fixative according to the laboratory procedure
-   Stained using JSB II followed by JSB I according to the Biolab method
-   Washed appropriately
-   Air dried before microscopy

Fixed smears may be stored for a longer period, although Biolab recommends staining as soon as reasonably possible.

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## Rapid Thin-Smear Staining Procedure

According to the current manufacturer insert:

1.  Prepare and air-dry the thin smear.
2.  Apply RAPID-MALARIA JSB stain fixative and allow the smear to dry.
3.  Dip the fixed smear in **Rapid-Malaria JSB II for approximately 3–5 seconds**.
4.  Wash in running tap water.
5.  Dip the smear in **Rapid-Malaria JSB I for approximately 35–45 seconds**.
6.  Wash in running tap water.
7.  Allow the smear to air dry.
8.  Examine under oil immersion for malaria parasites.

Biolab notes that staining time may be increased or decreased depending on the individual staining result.

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## Thick Blood Smear Application

Thick smears are useful where a greater volume of blood is examined microscopically.

The Biolab procedure includes a **dehaemoglobinisation step** for thick smears before staining.

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## Rapid Thick-Smear Procedure

1.  Prepare an air-dried thick smear.
2.  Keep the smear side facing upward.
3.  Add distilled water and allow approximately **1–2 minutes** for dehaemoglobinisation.
4.  Carefully remove the water.
5.  Allow the slide to air dry.
6.  Fix according to the manufacturer's prescribed procedure.
7.  Dip the smear in **Rapid-Malaria JSB II for approximately 5–6 seconds**.
8.  Wash in running tap water.
9.  Dip in **Rapid-Malaria JSB I for approximately 35–45 seconds**.
10.  Wash in running tap water.
11.  Dry and examine under oil immersion.

**Always follow the current pack insert supplied with the actual reagent lot, particularly for smear fixation and thick-smear processing.**

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## Expected Microscopic Staining Appearance

| Structure | Manufacturer-Described Appearance |
| --- | --- |
| **Leucocyte Nuclei** | Varying shades of dark blue |
| **Leucocyte Cytoplasm** | Pale or light blue |
| **Red Blood Cells** | Pale reddish |
| **Malaria Parasites** | Varying shades of blue |
| **Schüffner's Dots** | Dark bluish-red |
| **Platelets** | Pale or dark blue |

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## Bulk Slide Staining

The Biolab IFU also describes bulk staining using staining tanks / slide holders.

This may be useful for:

-   Hospital laboratories
-   High-volume malaria screening
-   Public-health laboratories
-   Teaching laboratories
-   Batch processing of multiple slides

The exact bulk-staining sequence and timings should follow the current Biolab pack insert.

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## Storage & Stability

The manufacturer insert states that the reagents are stable when stored at approximately **25–35°C** for up to **60 months**, subject to the expiry printed on the actual supplied product.

Important storage precautions include:

-   Keep bottles tightly closed.
-   Protect reagents from contamination.
-   Store within the manufacturer-specified temperature range.
-   Keep the fixative away from heat and fire.
-   Do not use reagents after the printed expiry date.

Biolab also notes that JSB stain may become exhausted after prolonged use, particularly after staining a large number of smears.

If staining becomes progressively lighter, the reagent should be replaced according to laboratory QC practice.

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## Quality Control

The manufacturer recommends periodically checking stain performance using:

-   Known positive malaria samples
-   Known negative samples

Laboratories should maintain appropriate staining controls and documented QC procedures.

Stain performance should be reviewed whenever:

-   Staining becomes unusually light
-   Background appearance changes
-   Parasite morphology becomes difficult to distinguish
-   A new lot is opened
-   Reagent contamination is suspected

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## Important Limitations

Microscopic malaria examination depends on more than the staining reagent alone.

The Biolab IFU specifically notes that accuracy also depends on:

-   Correct staining performance
-   Professional experience
-   Proper smear preparation
-   A good optical microscope system
-   Appropriate magnification
-   Ability to clearly identify parasite morphology

A negative smear does not always exclude malaria, particularly when parasitaemia is low or specimen timing / preparation is suboptimal.

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## Product Specifications

|     |     |
| --- | --- |
| **Product Name** | Rapid Malaria Stain Kit JSB I & II |
| **Brand** | Biolab Diagnostics |
| **Main Pack Size** | 2 × 500 mL |
| **JSB I** | 500 mL |
| **JSB II** | 500 mL |
| **Fixative Listed in IFU** | Easyfix Peripheral Smear Fixative – 50 mL; verify current supplied pack |
| **Method** | Rapid JSB Differential Staining |
| **Primary Application** | Malaria Parasite / Haemoparasite Microscopy |
| **Specimen** | Peripheral Blood Smear / EDTA Blood |
| **Thin Smear JSB II** | Approx. 3–5 Seconds |
| **Thin Smear JSB I** | Approx. 35–45 Seconds |
| **Thick Smear JSB II** | Approx. 5–6 Seconds |
| **Thick Smear JSB I** | Approx. 35–45 Seconds |
| **Microscopy** | Oil Immersion |
| **Working Reagent** | Ready to Use |
| **Storage** | Manufacturer IFU states approximately 25–35°C; follow actual pack label |
| **Stability** | Up to approximately 60 Months according to IFU; verify actual expiry |
| **Intended User** | Professional Laboratory Personnel |

* * *

## Benefits of Biolab Rapid Malaria JSB Stain

-   **Rapid Staining:** Designed for fast malaria parasite staining
-   **Ready to Use:** No working-reagent preparation required
-   **Thin & Thick Smears:** Supports both common malaria microscopy formats
-   **Large 500 mL Packs:** Suitable for routine and higher-volume laboratories
-   **Differential Colour Contrast:** Helps visualise parasites and blood-cell morphology
-   **Manual or Bulk Workflow:** Suitable for Coplin jars and slide-rack staining
-   **Long Stability:** Manufacturer lists extended stability under prescribed storage
-   **Professional Malaria Screening:** Suitable for laboratory microscopy programmes

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## JSB Rapid Malaria Stain vs Conventional Longer Staining Methods

| Feature | Biolab Rapid JSB I & II | Conventional Longer Staining Method |
| --- | --- | --- |
| **Workflow** | Rapid stain sequence | Usually longer stain contact time |
| **Thin Smear** | Supported | Supported depending on method |
| **Thick Smear** | Supported | Supported depending on method |
| **Reagent Preparation** | Ready to use | Method dependent |
| **Best Use** | Rapid routine microscopy | Depends on laboratory SOP |

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## Why Buy Biolab Rapid Malaria Stain from Microsidd India?

Malaria staining reagents can differ significantly in **method, stain composition, staining sequence, timing, pack size and smear compatibility**.

Microsidd clearly identifies this product as **Biolab Rapid Malaria Stain Kit – JSB I & II – 2 × 500 mL**.

| Buying Factor | Microsidd India | General Online Marketplace | Typical Offline Store |
| --- | --- | --- | --- |
| **Exact Method** | JSB I & II clearly stated | May be listed simply as malaria stain | Depends on staff familiarity |
| **Pack Size** | 2 × 500 mL clearly stated | May differ by seller | Depends on local stock |
| **Procedure Information** | Thin- and thick-smear applications explained | Listing detail varies | Usually dependent on staff knowledge |
| **IFU Verification** | Manufacturer instructions emphasised | Seller dependent | Store dependent |
| **GST Invoice** | Available for eligible orders | Seller dependent | Store dependent |
| **Bengaluru Presence** | RT Nagar, Bengaluru | Seller may be located elsewhere | Depends on local availability |
| **India-Wide Ordering** | Available to serviceable locations | Platform dependent | Usually local purchase |

* * *

## Buy Rapid Malaria Stain in Bangalore

**Microsidd India** supplies laboratory stains, malaria microscopy reagents, diagnostic reagents and professional laboratory consumables from **RT Nagar, Bengaluru**.

Customers searching for **malaria stain kit Bangalore**, JSB stain Bengaluru, Biolab Rapid Malaria Stain near me, blood parasite stain RT Nagar, malaria microscopy stain Bangalore, JSB I & II supplier Karnataka or Biolab Diagnostics supplier Bengaluru can check current availability through Microsidd.com.

Laboratories and institutions requiring multiple bottles should confirm current stock, batch and expiry before ordering.

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## Microsidd India – NAP

**Business Name:** Microsidd India  
**Address:** #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India  
**Phone / WhatsApp:** +91 63667 72288  
**Email:** support@microsidd.com  
**Website:** https://microsidd.com  
**Working Hours:** Monday to Saturday, 10:00 AM – 6:00 PM IST

[**View Microsidd India on Google Maps**](https://share.google/NWhhEfmvlEyIfAShq)

Browse related laboratory products in [**Microsidd Laboratory Needs**](https://microsidd.com/collections/laboratory-needs).

Browse professional reagents in [**Microsidd Laboratory Reagents**](https://microsidd.com/collections/bio-chemistry-reagents).

For local ordering information, visit [**Microsidd India – Laboratory & Medical Supplies Bangalore**](https://microsidd.com/pages/microsidd-india-bangalore).

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## Frequently Asked Questions – Biolab Rapid Malaria Stain JSB I & II

### What is the exact product?

This listing is for **Biolab Rapid Malaria Stain Kit – JSB I & II**.

### What is the pack size?

The main stain pack contains **JSB I 500 mL + JSB II 500 mL**.

### Is a fixative included?

The supplied Biolab IFU lists an Easyfix Peripheral Smear Fixative 50 mL. Because pack configurations may change, confirm the actual current commercial pack if the fixative is specifically required.

### What is this stain used for?

It is designed for rapid staining of peripheral blood smears for microscopic demonstration of malaria parasites and other blood-cell structures.

### Can it be used for thin blood smears?

Yes. Biolab provides a dedicated thin-smear staining procedure.

### Can it be used for thick blood smears?

Yes. The IFU also provides a procedure for dehaemoglobinised thick smears.

### Does it require dilution?

No. Biolab describes the JSB reagents as ready to use.

### What stains are used?

The system is based primarily on polychromated Methylene Blue and Eosin staining.

### How quickly can a thin smear be stained?

The manufacturer procedure uses approximately 3–5 seconds in JSB II followed by approximately 35–45 seconds in JSB I, excluding fixation, washing and drying.

### Why is it called a one-minute malaria stain?

The principal stain-contact steps can be completed rapidly, with the combined stain exposure around one minute or less under the manufacturer's procedure. Total workflow time also includes fixation, washing, drying and microscopy.

### How is a thick smear handled?

The Biolab method includes dehaemoglobinisation with distilled water before the rapid staining sequence.

### How are malaria parasites expected to appear?

Biolab describes malaria parasites as appearing in varying shades of blue under the specified stain.

### What colour are RBCs?

The manufacturer describes red blood cells as pale reddish.

### What colour are leucocyte nuclei?

They are described as varying shades of dark blue.

### Can EDTA blood be used?

Yes. Biolab states that EDTA-anticoagulated blood may be used without substantial change in staining results.

### What microscope is required?

The stained smear is examined using a suitable laboratory microscope under oil immersion according to the manufacturer procedure.

### How should stain performance be checked?

Biolab recommends periodic checking using known positive and negative samples.

### How should the stains be stored?

The supplied IFU states approximately 25–35°C. Keep reagents appropriately capped and follow the actual storage instructions printed on the current pack.

### How long are the reagents stable?

The manufacturer insert states up to approximately 60 months under prescribed conditions. Always use the printed expiry date on the supplied batch as the final reference.

### Can exhausted stain still be used?

No. Biolab advises replacing the stain if prolonged use produces progressively lighter staining.

### Can malaria be ruled out by one negative smear?

No. Microscopy sensitivity depends on parasite density, specimen timing, smear quality, staining and examiner expertise. Clinical evaluation and repeat / alternative testing may be required.

### Is this suitable for home use?

No. This product is intended for trained professional laboratory personnel.

### Where can I buy Biolab JSB Malaria Stain in Bangalore?

Biolab Rapid Malaria Stain JSB I & II can be ordered through Microsidd India in RT Nagar, Bengaluru, subject to current stock and batch availability.

### Does Microsidd supply laboratories outside Bengaluru?

Yes. Microsidd supplies professional laboratory products to serviceable locations across India, subject to stock and courier serviceability.

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## Medical & Laboratory Disclaimer

**Biolab Rapid Malaria Stain JSB I & II is a professional laboratory staining reagent and should be used only by trained personnel according to the current manufacturer IFU, laboratory SOP, biosafety procedures and microscopy QC requirements.**

A stained blood smear is one component of malaria laboratory investigation.

Result quality may be affected by:

-   Specimen timing
-   Parasite density
-   Smear thickness
-   Fixation
-   Dehaemoglobinisation
-   Staining time
-   Stain condition
-   Washing technique
-   Microscope optics
-   Oil-immersion technique
-   Operator experience

A negative microscopy result does not automatically exclude malaria where clinical suspicion remains high.

Diagnostic interpretation and subsequent patient management should be performed by appropriately qualified healthcare and laboratory professionals.

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## No Return / No Exchange – Verify Method, Pack & Application Before Ordering

**This is a professional laboratory reagent and is method-, application-, storage- and expiry-sensitive.**

Please verify before ordering:

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid Malaria Stain Kit
-   **Method:** JSB I & II
-   **Pack:** 2 × 500 mL main stains
-   Required smear type
-   Laboratory staining method
-   Fixative requirement
-   Current batch and expiry

A correctly supplied reagent cannot normally be returned or exchanged because a different staining method, pack configuration, application or laboratory SOP was required.

Laboratory reagents are storage-sensitive, expiry-sensitive and contamination-sensitive products and cannot normally be returned to saleable stock once correctly delivered.

Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the [**Microsidd Refund, Return, Replacement & Cancellation Policy**](https://microsidd.com/pages/cancellation-and-refunds).

**An original, continuous, unedited and uncut unboxing video is compulsory for delivery-related claims involving an incorrect, missing or damaged product.**

Begin recording before opening the parcel. Open the outer carton carefully by **cutting the sealing tape with a blade or suitable cutter; do not tear the carton**. Keep the courier label, sealed Biolab packaging, reagent bottles, pack configuration, batch and expiry continuously visible.

* * *

## Biolab Rapid Malaria Stain JSB I & II –  Product Entity Summary

**Product:** Rapid Malaria Stain Kit  
**Brand:** Biolab Diagnostics  
**Method:** JSB I & II Rapid Differential Staining  
**Pack:** JSB I 500 mL + JSB II 500 mL  
**IFU-Listed Fixative:** Easyfix Peripheral Smear Fixative 50 mL; verify current supplied configuration  
**Primary Application:** Malaria Parasite Microscopy  
**Specimen:** Peripheral Blood Smears / EDTA Blood  
**Smear Types:** Thin & Thick Blood Smears  
**Main Stain Chemistry:** Polychromated Methylene Blue & Eosin  
**Preparation:** Ready to Use  
**Microscopy:** Oil Immersion  
**Storage:** Manufacturer IFU states approximately 25–35°C; verify current pack  
**Intended User:** Professional Laboratory Personnel  
**Seller:** Microsidd India  
**Seller Location:** RT Nagar, Bengaluru, Karnataka, India

## Details

- **Price:** 966.0 INR
- **Vendor:** Biolab
- **Type:** Haematology & Parasitology Stain

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| Default Title | 966.00 INR | In stock |

## Images

- Biolab Rapid Malaria Stain Kit JSB I and II 2x500 ml for malaria parasite microscopy
- Biolab Rapid Malaria Stain Kit JSB I and II 2x500 ml for malaria parasite microscopy
- Biolab Rapid Malaria Stain Kit JSB I and II 2x500 ml for malaria parasite microscopy
- Biolab Rapid Malaria Stain Kit JSB I and II 2x500 ml for malaria parasite microscopy
- Biolab Rapid Malaria Stain Kit JSB I and II 2x500 ml for malaria parasite microscopy

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> Source: [Microsidd India](https://microsidd.com/products/biolab-rapid-malaria-stain-jsb-i-ii-2x500ml)
> Updated: 2026-11-11