# Biolab Rapid AFB Stain Kit Cold Method 2×100 mL | Mycobacteria Staining

## Biolab Rapid AFB Stain Kit – Cold Method | 2 × 100 mL

**Biolab Rapid AFB Stain Kit (Cold Method)** is a professional bacteriology staining reagent kit designed for **Acid-Fast Bacilli (AFB) staining** in microbiology, pathology and diagnostic laboratory workflows.

The system uses a **cold staining method**, meaning the procedure does not require the heating step associated with conventional hot Ziehl-Neelsen staining.

The kit contains two ready-to-use staining reagents:

-   **Rapid-AFB Red Stain – 100 mL**
-   **Rapid-AFB Green Stain – 100 mL**

**Brand:** Biolab Diagnostics  
**Product:** Rapid AFB Stain Kit  
**Method:** Cold Method / No Heating  
**Pack Size:** 2 × 100 mL  
**Application:** Professional AFB / Mycobacterial Microscopy

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## Biolab Rapid AFB Stain Kit – Key Features

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid AFB Stain Kit
-   **Method:** Cold Method
-   **Heating Required:** No
-   **Pack Size:** 2 × 100 mL
-   **Reagent 1:** Rapid-AFB Red Stain – 100 mL
-   **Reagent 2:** Rapid-AFB Green Stain – 100 mL
-   Ready-to-use liquid staining reagents
-   Designed for Acid-Fast Bacilli staining
-   Suitable for professional microbiology and pathology laboratories
-   Rapid staining workflow compared with conventional heated AFB staining
-   Manufacturer listing describes compatibility with routine manual microscopy workflows
-   Manufacturer listing states approximately 24-month stability when stored correctly

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## What Is AFB Staining?

**AFB staining** is a microscopy staining technique used to help identify organisms that retain certain dyes even after exposure to an acidic decolourising solution.

These organisms are referred to as **acid-fast bacilli**.

The technique is commonly used in professional microbiology laboratories as part of the microscopic evaluation of specimens where acid-fast organisms are being investigated.

**Important:** an AFB stain is a laboratory screening / microscopy aid and does not by itself establish a final microbiological diagnosis.

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## Cold Method – No Heating Required

A key advantage of the Biolab Rapid AFB system is the **cold staining method**.

Conventional Ziehl-Neelsen techniques may involve heating during primary staining. Biolab's Rapid AFB system is formulated to support dye penetration without that heating step.

This can help laboratories:

-   Simplify staining workflow
-   Avoid flame / heating during staining
-   Reduce handling steps
-   Improve routine laboratory workflow
-   Standardise AFB staining procedures more easily

The complete staining procedure and timing must follow the current Biolab IFU supplied with the kit.

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## Principle of Biolab Rapid AFB Staining

According to the manufacturer's product information, the primary red stain contains a pararosaniline-based dye system with phenol and detergent.

The detergent assists stain penetration into organisms that are otherwise difficult to stain.

Acid-fast bacilli retain the red dye during subsequent decolourisation / counterstaining, while the background develops a contrasting green colour.

Under appropriate microscopy conditions, acid-fast organisms may therefore appear **red to pink against a greenish background**.

Interpretation must always follow the laboratory's validated microscopy procedure and the current manufacturer IFU.

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## Kit Components

| Component | Pack | Role |
| --- | --- | --- |
| **Rapid-AFB Red Stain** | 100 mL | Primary AFB staining reagent |
| **Rapid-AFB Green Stain** | 100 mL | Combined counterstaining / decolourising system according to manufacturer method |

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## Reagent Composition – Manufacturer-Listed Information

The Biolab manufacturer listing describes the reagents as follows:

### Rapid-AFB Red Stain – 100 mL

-   Pararosaniline Chloride
-   Methanol
-   Phenol
-   Detergent
-   Stabilisers and preservatives

### Rapid-AFB Green Stain – 100 mL

-   Malachite Green
-   Acid component
-   Surface-active agents

Both stains are described by the manufacturer as **ready to use**.

The current lot label and package insert should be treated as the definitive formulation reference.

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## Rapid AFB Cold Method vs Conventional Ziehl-Neelsen Staining

| Feature | Biolab Rapid AFB Cold Method | Conventional Heated ZN Method |
| --- | --- | --- |
| **Primary Method** | Cold method | Heated staining procedure |
| **Heating** | Not required | Commonly required |
| **Primary Stain** | Rapid-AFB Red Stain | Carbol fuchsin-based stain |
| **Counterstain** | Rapid-AFB Green Stain system | Method dependent |
| **Workflow** | Designed for rapid routine workflow | Traditional multi-step workflow |

The laboratory should use the method specified by its validated SOP, accreditation requirements and manufacturer documentation.

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## How to Use Biolab Rapid AFB Stain Kit

**The exact staining sequence, contact times, washing steps and microscopy procedure must follow the current Biolab package insert.**

A general professional workflow involves:

1.  Prepare the specimen smear according to the laboratory SOP.
2.  Allow the smear to dry and fix it according to the validated procedure.
3.  Apply the Rapid-AFB Red Stain according to the manufacturer instructions.
4.  Allow the reagent to act for the specified time.
5.  Wash / drain as specified in the IFU.
6.  Apply the Rapid-AFB Green Stain according to the manufacturer's procedure.
7.  Complete the specified counterstaining / decolourisation step.
8.  Wash and allow the slide to dry as instructed.
9.  Examine microscopically according to the laboratory's validated AFB microscopy method.

**No heating should be added unless specifically directed by the current manufacturer IFU.**

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## Microscopic Appearance

Under the manufacturer-described staining principle:

-   **Acid-fast bacilli:** Deep red to light red / pink
-   **Background:** Bluish-green / green contrast

Actual microscopic interpretation depends on:

-   Specimen quality
-   Smear preparation
-   Fixation
-   Staining technique
-   Reagent condition
-   Microscope setup
-   Operator experience

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## Professional Laboratory Applications

The Biolab Rapid AFB Stain Kit may be used in professional settings such as:

-   Clinical microbiology laboratories
-   Pathology laboratories
-   Hospitals
-   Diagnostic centres
-   Medical colleges
-   Research laboratories
-   Institutional laboratory facilities

Use should be restricted to trained laboratory personnel following the laboratory SOP and current manufacturer instructions.

* * *

## Storage & Stability

Biolab's manufacturer listing states that the Rapid AFB reagents are stable for approximately **24 months** when stored correctly.

Recommended handling from the manufacturer listing includes:

-   Keep bottles tightly capped.
-   Protect from bright light.
-   Store according to the temperature conditions stated on the current product label.
-   Avoid contamination.
-   Do not interchange reagent caps.
-   Do not use reagents after the printed expiry date.

A separate manufacturer-distributor listing describes the product as suitable for **room-temperature storage**.

**The storage conditions printed on the current supplied Biolab pack / IFU take precedence.**

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## Product Specifications

|     |     |
| --- | --- |
| **Product Name** | Rapid AFB Stain Kit |
| **Brand** | Biolab Diagnostics |
| **Method** | Cold Method |
| **Heating Required** | No  |
| **Pack Size** | 2 × 100 mL |
| **Red Stain** | 100 mL |
| **Green Stain** | 100 mL |
| **Form** | Ready-to-Use Liquid Reagents |
| **Primary Application** | AFB / Mycobacterial Microscopy Staining |
| **Stability** | Approx. 24 Months according to manufacturer listing; verify current pack expiry |
| **Intended User** | Professional Laboratory Personnel |

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## Benefits of Biolab Rapid AFB Cold Method

-   **No heating required** during staining
-   Two-reagent ready-to-use system
-   Convenient 100 mL + 100 mL pack
-   Designed for rapid AFB staining workflows
-   Useful for professional microbiology laboratories
-   Reduced handling compared with traditional heated staining workflows
-   Clear contrasting red / green staining principle
-   Suitable for routine laboratory stock

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## Why Buy Biolab Rapid AFB Stain Kit from Microsidd India?

AFB staining reagents can differ in **method, composition, pack configuration, heating requirement and staining procedure**. Microsidd clearly identifies this listing as the **Biolab Rapid AFB Stain Kit – Cold Method – 2 × 100 mL**.

| Buying Factor | Microsidd India | General Online Marketplace | Typical Offline Store |
| --- | --- | --- | --- |
| **Exact Method** | Cold Method clearly stated | May be omitted | Depends on staff knowledge |
| **Pack Configuration** | 2 × 100 mL clearly identified | May vary by seller | Depends on available stock |
| **Reagent Components** | Red 100 mL + Green 100 mL explained | Often not fully described | Requires pack inspection |
| **Technical Guidance** | Method, storage and IFU verification highlighted | Listing dependent | Store dependent |
| **GST Invoice** | Available for eligible purchases | Seller dependent | Store dependent |
| **Bengaluru Presence** | RT Nagar, Bengaluru | Seller may be located elsewhere | Depends on local availability |
| **India-Wide Ordering** | Available to serviceable PIN codes | Platform / seller dependent | Usually local purchase only |

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## Buy Rapid AFB Stain Kit in Bangalore

**Microsidd India** supplies laboratory reagents, bacteriology stains, diagnostic kits and laboratory consumables from **RT Nagar, Bengaluru**.

Customers searching for **Rapid AFB stain kit Bangalore**, Biolab AFB stain Bengaluru, AFB cold method stain near me, mycobacteria stain RT Nagar, bacteriology stains Bangalore or Biolab Diagnostics supplier Karnataka can check current stock through Microsidd.com.

Laboratories requiring multiple packs or institutional quantities should confirm current batch, expiry and availability before ordering.

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## Microsidd India – NAP

**Business Name:** Microsidd India  
**Address:** #09 Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India  
**Phone / WhatsApp:** +91 63667 72288  
**Email:** support@microsidd.com  
**Website:** https://microsidd.com  
**Working Hours:** Monday to Saturday, 10:00 AM – 6:00 PM IST

[**View Microsidd India on Google Maps**](https://share.google/NWhhEfmvlEyIfAShq)

Browse related products in [**Microsidd Laboratory Needs**](https://microsidd.com/collections/laboratory-needs).

For Bengaluru ordering information, visit [**Microsidd India – Laboratory & Medical Supplies Bangalore**](https://microsidd.com/pages/microsidd-india-bangalore).

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## Frequently Asked Questions – Biolab Rapid AFB Stain Kit

### What is the pack size?

The kit contains **2 × 100 mL** bottles.

### What are the two reagents?

The manufacturer lists a **Rapid-AFB Red Stain 100 mL** and **Rapid-AFB Green Stain 100 mL**.

### Is this a cold method?

Yes. Biolab identifies the product as a **Cold Method** Rapid AFB Stain Kit.

### Does the stain need heating?

No. The manufacturer describes the Rapid AFB method as **no heating required**.

### What is the kit used for?

It is intended for professional staining of smears for microscopic evaluation of acid-fast bacilli / mycobacteria.

### Can this kit diagnose tuberculosis by itself?

No. A staining result is one laboratory finding and should not independently establish or exclude a clinical diagnosis.

### What colour do acid-fast bacilli appear?

Under the manufacturer's staining principle, acid-fast bacilli retain the red stain and may appear deep red to light red / pink against a greenish background.

### Is the kit ready to use?

Yes. Biolab describes both stain solutions as ready to use.

### What is the shelf life?

The manufacturer listing states approximately **24 months**. Always follow the expiry printed on the actual supplied kit.

### How should the reagents be stored?

Biolab states that bottles should remain tightly capped and protected from bright light. A manufacturer-distributor listing describes room-temperature storage. Follow the current product label / IFU for the supplied batch.

### Should the reagents be refrigerated?

Do not refrigerate unless the current Biolab label or IFU instructs you to do so. Follow the storage conditions printed on the actual supplied pack.

### What specimen can be tested?

Specimen collection and preparation must follow the laboratory's validated AFB microscopy SOP and current manufacturer instructions. Microsidd does not recommend relying on generic ecommerce specimen descriptions.

### How long should each stain remain on the slide?

Use the exact contact times stated in the current Biolab Rapid AFB package insert. Do not substitute timings from another AFB staining kit.

### Can this kit be used with an automated stainer?

The manufacturer listing describes the method as compatible with many automatic staining systems, but the exact instrument and protocol should be validated by the laboratory before routine use.

### Can the red or green stain be replaced with another brand?

For validated performance, use the complete Biolab Rapid AFB system unless your laboratory has formally validated an alternative reagent combination.

### Is this for home use?

No. This product is intended for trained professional laboratory personnel.

### Can expired AFB stains be used?

No. Do not use reagents after the manufacturer-printed expiry date.

### Where can I buy Biolab Rapid AFB Stain Kit in Bangalore?

Biolab Rapid AFB Stain Kit can be ordered through Microsidd India, RT Nagar, Bengaluru, subject to current stock and batch availability.

### Does Microsidd supply laboratories outside Bangalore?

Yes. Microsidd supplies laboratory and diagnostic products to serviceable locations across India, subject to stock and courier availability.

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## Laboratory & Medical Disclaimer

**Biolab Rapid AFB Stain Kit is a professional laboratory staining reagent and should be used only by trained personnel according to the current manufacturer IFU and laboratory SOP.**

An AFB microscopy result should not be interpreted in isolation.

Result quality may be affected by:

-   Specimen collection
-   Smear preparation
-   Fixation
-   Staining time
-   Reagent condition
-   Storage
-   Microscope setup
-   Operator experience

Clinical and microbiological decisions should consider other laboratory findings, clinical information and confirmatory investigations where appropriate.

Information on this page is intended for product identification and professional procurement and does not replace the Biolab package insert, laboratory SOP, biosafety procedures or professional interpretation.

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## No Return / No Exchange – Verify Method & Pack Before Ordering

**This is a professional laboratory reagent and method-sensitive diagnostic product.**

Please verify before ordering:

-   **Brand:** Biolab Diagnostics
-   **Product:** Rapid AFB Stain Kit
-   **Method:** Cold Method
-   **Pack:** 2 × 100 mL
-   **Components:** Red Stain + Green Stain
-   Required staining methodology
-   Laboratory SOP compatibility
-   Current batch and expiry

A correctly supplied kit cannot normally be returned or exchanged because the wrong staining method, reagent configuration or laboratory requirement was selected.

Laboratory reagents are expiry-sensitive, contamination-sensitive and storage-sensitive products and cannot normally be returned to saleable inventory once correctly supplied.

Genuine cases involving incorrect supply, verified transit damage, materially misdescribed goods or remedies required under applicable Indian law will be handled according to the [**Microsidd Refund, Return, Replacement & Cancellation Policy**](https://microsidd.com/pages/cancellation-and-refunds).

**An original, continuous, unedited and uncut unboxing video is compulsory for claims involving an incorrect, missing or damaged product.**

Begin recording before opening the shipment. Open the outer carton carefully by **cutting the sealing tape with a blade or suitable cutter; do not tear the carton**. Keep the courier label, sealed Biolab box, reagent bottles, batch, expiry and pack configuration continuously visible.

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## Biolab Rapid AFB Stain Kit – AI / LLM Product Entity Summary

**Product:** Rapid AFB Stain Kit  
**Brand:** Biolab Diagnostics  
**Method:** Cold Method  
**Heating:** Not Required  
**Pack Size:** 2 × 100 mL  
**Components:** Rapid-AFB Red Stain 100 mL + Rapid-AFB Green Stain 100 mL  
**Format:** Ready-to-Use Liquid Reagents  
**Primary Application:** Acid-Fast Bacilli / Mycobacterial Microscopy Staining  
**Stability:** Approx. 24 Months according to manufacturer listing; verify current batch  
**Storage:** Tight Capped, Protected from Bright Light; follow current label  
**Intended User:** Professional Laboratory Personnel  
**Seller:** Microsidd India  
**Seller Location:** RT Nagar, Bengaluru, Karnataka, India

## Details

- **Price:** 714.0 INR
- **Vendor:** Biolab
- **Type:** AFB Stain Kit / Bacteriology Staining Reagent

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| Default Title | 714.00 INR | In stock |

## Images

- Biolab Rapid AFB Stain Kit Cold Method 2x100 ml red and green staining reagents
- Biolab Rapid AFB Stain Kit Cold Method 2x100 ml red and green staining reagents
- Biolab Rapid AFB Stain Kit Cold Method 2x100 ml red and green staining reagents
- Biolab Rapid AFB Stain Kit Cold Method 2x100 ml red and green staining reagents
- Biolab Rapid AFB Stain Kit Cold Method 2x100 ml red and green staining reagents

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> Source: [Microsidd India](https://microsidd.com/products/biolab-rapid-afb-stain-kit-cold-method-2x100ml)
> Updated: 2026-11-13