# BIOLAB Hematoxylin (Harris) 100ml / 500ml | H&E & PAP Nuclear Stain

## BIOLAB Hematoxylin (Harris) 100ml / 500ml – H&E & PAP Nuclear Stain

**BIOLAB Hematoxylin (Harris)** is a professional histology and cytology staining reagent supplied in a **100 mL & 500 mL pack** for nuclear staining in validated laboratory procedures.

BIOLAB lists this product in its cytology staining range as **“HEMATOXYLENE (HARRIS)”**, while its analytical-stain catalogue identifies Harris hematoxylin for **PAP and H&E staining**.

Harris hematoxylin is widely used as a **nuclear stain**. In properly performed staining procedures, cell nuclei generally develop a blue to blue-purple appearance, providing contrast against cytoplasmic and extracellular counterstains such as eosin.

**Professional laboratory use only.** Specimen fixation, tissue processing, section preparation, staining time, differentiation, bluing, counterstaining, dehydration, clearing, mounting and microscopic interpretation must follow the current BIOLAB instructions and the laboratory's validated SOP.

Vendor: **BIOLAB Diagnostics**. Product type: **Histopathology / Cytology Stain**

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## BIOLAB Harris Hematoxylin – Key Features

-   **Brand:** BIOLAB Diagnostics
-   **Product:** Hematoxylin (Harris)
-   **Manufacturer Product Name:** HEMATOXYLENE (HARRIS)
-   **Pack Size:** 100 mL & 500 mL
-   Professional nuclear staining reagent
-   Suitable for validated **Hematoxylin & Eosin (H&E)** workflows
-   Suitable for appropriate **Papanicolaou / PAP staining** workflows
-   Produces blue to blue-purple nuclear contrast when used correctly
-   Suitable for histopathology, cytology, pathology, teaching and research laboratories
-   Can be paired with an appropriate eosin counterstain in H&E workflows
-   Designed for professional laboratory microscopy
-   **Not intended for home self-testing**

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## Product Specifications

|     |     |
| --- | --- |
| **Product Name** | Hematoxylin (Harris) |
| **Manufacturer Listing** | HEMATOXYLENE (HARRIS) |
| **Brand** | BIOLAB Diagnostics |
| **Pack Size** | 100 mL & 500 mL |
| **Product Category** | Histology / Cytology Staining Reagent |
| **Primary Function** | Nuclear Staining |
| **Typical Staining Workflows** | H&E and PAP staining |
| **Expected Nuclear Appearance** | Blue to Blue-Purple following appropriate staining and bluing |
| **Use Environment** | Professional Histology / Cytology Laboratory |

**Important:** Exact formulation, staining time, differentiation requirement, bluing procedure, storage, stability and expiry must be checked on the current BIOLAB product label and package documentation supplied with your batch.

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## What Is Harris Hematoxylin?

Harris hematoxylin is a widely used formulation of hematoxylin for staining cellular nuclei in histological and cytological specimens.

Hematoxylin itself undergoes oxidation to form **hematein**. In a suitable staining formulation, hematein complexes with a metallic mordant and interacts with nuclear structures, creating the characteristic blue-purple nuclear staining seen after appropriate processing and bluing.

The purpose of nuclear staining is to provide contrast that allows trained laboratory professionals and pathologists to evaluate cellular and tissue morphology under a microscope.

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## Why Does Hematoxylin Stain Nuclei Blue?

Hematoxylin staining depends on the interaction between oxidised hematoxylin, the mordant and negatively charged cellular components associated with nuclear chromatin.

Following appropriate staining and **bluing**, nuclei typically appear:

-   Blue
-   Blue-purple
-   Dark blue depending on staining intensity and protocol

The final colour can vary according to:

-   Specimen fixation
-   Section thickness
-   Hematoxylin condition
-   Staining time
-   Differentiation
-   Water pH
-   Bluing procedure
-   Counterstaining
-   Microscope settings

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## Harris Hematoxylin in H&E Staining

**Hematoxylin & Eosin (H&E)** is one of the most widely used staining combinations in routine histology.

The two stains provide complementary contrast:

-   **Hematoxylin:** primarily highlights nuclei in blue to blue-purple tones.
-   **Eosin:** counterstains many cytoplasmic and extracellular protein structures in pink to red tones.

Together they help provide a general overview of tissue architecture and cellular morphology.

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## Typical H&E Workflow

A conventional H&E laboratory workflow may involve:

1.  Tissue fixation
2.  Processing and embedding where applicable
3.  Sectioning
4.  Deparaffinisation where required
5.  Hydration
6.  Hematoxylin staining
7.  Washing
8.  Differentiation where required
9.  Bluing
10.  Eosin counterstaining
11.  Dehydration
12.  Clearing
13.  Mounting
14.  Microscopic examination

**Do not use a generic staining time as the operating procedure for this BIOLAB reagent.** Exact exposure times and sequence should follow the current BIOLAB instructions or your laboratory's validated SOP.

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## Harris Hematoxylin in PAP Staining

BIOLAB also identifies Harris hematoxylin for use in **PAP / Papanicolaou staining** workflows.

Within an appropriate PAP staining procedure, hematoxylin is used to provide nuclear staining before subsequent cytoplasmic staining steps.

PAP staining can involve multiple reagents and carefully controlled timing. Laboratories should verify the complete protocol and compatibility of all staining solutions before routine use.

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## Harris Hematoxylin vs Eosin

| Feature | Harris Hematoxylin | Eosin |
| --- | --- | --- |
| **Main Role** | Nuclear stain | Cytoplasmic / extracellular counterstain |
| **Typical Colour** | Blue / Blue-Purple | Pink / Red |
| **Used Together** | Commonly combined in H&E staining |     |

Microsidd also supplies professional staining and laboratory reagents through its [**Laboratory Reagents & Chemicals Collection**](https://microsidd.com/collections/chemicals-liquids).

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## Why Is Differentiation Important?

Harris hematoxylin is commonly used in staining systems where differentiation may be required to remove excess or non-specific background staining and improve nuclear definition.

The laboratory should use only the differentiation procedure specified in the validated method.

Over-differentiation can produce weak nuclear staining, while inadequate differentiation may result in excessive background or poor nuclear contrast.

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## What Is Bluing?

After hematoxylin staining, a **bluing step** is commonly used to convert the stained nuclear colour toward the characteristic blue appearance.

Bluing can be affected by water chemistry and pH. Therefore laboratories should maintain consistent validated conditions rather than assuming all tap-water supplies will behave identically.

Poor or incomplete bluing may produce nuclei that appear reddish, purple-brown or otherwise inconsistent with the expected staining pattern.

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## Factors That Affect Hematoxylin Staining Quality

High-quality histology staining depends on the complete process, not simply on the reagent bottle.

Important factors include:

-   Fixation quality
-   Specimen processing
-   Section thickness
-   Slide cleanliness
-   Reagent age and condition
-   Reagent contamination
-   Staining time
-   Differentiation time
-   Bluing
-   Water quality and pH
-   Counterstaining
-   Dehydration and clearing
-   Mounting
-   Operator technique

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## Who Uses BIOLAB Harris Hematoxylin?

The reagent is relevant to professional laboratory environments including:

-   Histopathology laboratories
-   Pathology laboratories
-   Cytology laboratories
-   Hospital laboratories
-   Medical colleges
-   Histology teaching laboratories
-   Cytology teaching laboratories
-   Research laboratories
-   Institutional laboratory procurement departments

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## Microscope Slides for Histology & Cytology

Correct slide preparation is essential for dependable staining and microscopy.

Microsidd supplies [**Microscope Glass Slides**](https://microsidd.com/products/microscope-glass-slides) for professional specimen preparation.

You can also browse the [**Microscope Slides & Laboratory Glassware Collection**](https://microsidd.com/collections/microscope-glass-slides-glass) for additional microscopy products.

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## Explore Laboratory Reagents at Microsidd

BIOLAB Harris Hematoxylin forms part of Microsidd India's wider professional laboratory-reagent range.

Explore:

-   [**Laboratory Reagents & Chemicals**](https://microsidd.com/collections/chemicals-liquids)
-   [**Laboratory Needs**](https://microsidd.com/collections/laboratory-needs)
-   [**Microscope Glass Slides**](https://microsidd.com/products/microscope-glass-slides)

This allows histology, pathology and cytology laboratories to source stains, slides, consumables and other laboratory products from the same specialist supplier.

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## Storage, Expiry & Handling

Store BIOLAB Harris Hematoxylin according to the exact storage conditions printed on the current manufacturer label and package documentation.

Good laboratory practice includes:

-   Keep the bottle securely closed when not in use
-   Prevent contamination of the stock reagent
-   Do not return used stain to the original bottle unless specifically permitted by SOP
-   Use clean staining vessels
-   Protect the reagent from unsuitable heat or light where specified
-   Observe the labelled storage temperature
-   Do not use beyond the labelled expiry date
-   Inspect reagent appearance before use according to laboratory procedure
-   Follow appropriate laboratory PPE requirements

If information on the supplied BIOLAB label differs from information displayed online, follow the current manufacturer's documentation supplied with the reagent.

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## Benefits of BIOLAB Harris Hematoxylin 100ml

-   Professional nuclear staining reagent
-   Suitable for H&E staining workflows
-   Suitable for applicable PAP staining workflows
-   100 mL pack suitable for lower-volume or specialised laboratory requirements
-   Useful for pathology, histology, cytology and teaching laboratories
-   Can be sourced together with eosin, microscope slides and other laboratory supplies
-   BIOLAB brand identification simplifies institutional procurement

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## Why Buy BIOLAB Harris Hematoxylin from Microsidd India?

Laboratory staining reagents should be purchased by checking the **exact stain formulation, brand, pack size, application and staining protocol**.

Microsidd India combines online ordering with a Bengaluru-based diagnostic and laboratory-products business and provides detailed product information to help professional buyers verify the correct reagent before ordering.

| Buying Consideration | Microsidd India | General Local Market / Marketplace |
| --- | --- | --- |
| **Exact Product Identification** | BIOLAB Harris Hematoxylin clearly identified | Description quality varies by seller |
| **Pack Size** | 100 mL clearly stated | Pack variants may not always be clearly separated |
| **Application Information** | H&E, PAP and nuclear-staining context explained | May be limited to a short product title |
| **Related Products** | Laboratory stains, reagents, microscope slides and consumables available | Depends on seller catalogue |
| **GST Invoice** | Available for eligible purchases | Seller dependent |
| **Bengaluru Presence** | Physical business presence in RT Nagar, Bengaluru | Depends on individual seller |
| **Institutional Procurement** | Laboratory and bulk requirements may be supported subject to stock | Varies by seller |
| **India-Wide Ordering** | Delivery to serviceable PIN codes across India | Depends on marketplace and individual seller |

Microsidd focuses on clearly identifying reagent type, pack size and intended professional application rather than relying only on a product photograph or abbreviated marketplace description.

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## Buy BIOLAB Harris Hematoxylin 100ml in Bangalore

**Microsidd India** is a diagnostic, medical and laboratory-products supplier based in **RT Nagar, Bengaluru**.

Laboratories searching for **Harris Hematoxylin in Bangalore**, **BIOLAB Hematoxylin 100ml Bengaluru**, H&E stain supplier Bangalore, histopathology stains Bengaluru, cytology stain supplier near RT Nagar, PAP staining reagents or professional laboratory staining reagents can check current availability through Microsidd.com.

For a particular batch, required remaining shelf life or local pickup, checking current stock before ordering or visiting is recommended.

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## Microsidd India – NAP

**Business Name:** Microsidd India  
**Address:** #09, Green Chambers, 14th Cross, RT Nagar, Bengaluru – 560032, Karnataka, India  
**Phone / WhatsApp:** +91 63667 72288  
**Email:** support@microsidd.com  
**Website:** https://microsidd.com  
**Working Hours:** Monday to Saturday, 10:00 AM – 6:00 PM IST

[**View Microsidd India on Google Maps**](https://www.google.com/maps/place/Microsidd+India/@13.0193885,77.5967849,17z/data=!4m14!1m7!3m6!1s0x3bae3d88d30dba57:0x8fc1ceef7bbff265!2sMicrosidd+India!8m2!3d13.0193885!4d77.5967849!16s%2Fg%2F11bw7cvpyd!3m5!1s0x3bae3d88d30dba57:0x8fc1ceef7bbff265!8m2!3d13.0193885!4d77.5967849!16s%2Fg%2F11bw7cvpyd?entry=ttu)

For Bengaluru business information, visit [**Microsidd India – Medical & Laboratory Supplies Bangalore**](https://microsidd.com/pages/microsidd-india-bangalore).

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## Frequently Asked Questions – BIOLAB Harris Hematoxylin 100ml

### What is Harris Hematoxylin?

Harris Hematoxylin is a professional nuclear staining reagent used in histology and cytology workflows.

### What is the BIOLAB product called?

BIOLAB currently lists the product as **HEMATOXYLENE (HARRIS)**. Microsidd uses the standard searchable spelling “Hematoxylin (Harris)” while retaining the manufacturer's terminology in the description.

### What is the pack size?

This Microsidd listing is for **100 mL**.

### What does Harris Hematoxylin stain?

It is primarily used to stain cellular nuclei, producing blue to blue-purple nuclear contrast following appropriate staining and bluing.

### Can Harris Hematoxylin be used for H&E staining?

Yes. BIOLAB identifies Harris hematoxylin for H&E staining applications.

### Can it be used for PAP staining?

Yes. BIOLAB's analytical-stain catalogue also identifies Harris hematoxylin for PAP staining workflows.

### Does this product include Eosin?

No. This listing is for **BIOLAB Harris Hematoxylin 100 mL only**. Eosin is a separate counterstaining reagent unless explicitly included in a kit.

### What colour should nuclei appear after staining?

Properly stained and blued nuclei generally appear blue to blue-purple. Exact appearance depends on the specimen, formulation and validated laboratory protocol.

### Why is a bluing step required?

Bluing helps convert the hematoxylin-stained nuclei toward the characteristic blue appearance and is an important controlled step in many hematoxylin procedures.

### Does Harris Hematoxylin require differentiation?

Harris hematoxylin is commonly used in procedures that include a differentiation step. The exact requirement depends on the formulation and validated laboratory protocol, so follow the current BIOLAB instructions.

### Can I use acid alcohol for differentiation?

Acid-alcohol differentiation is used in many Harris hematoxylin protocols, but do not assume a concentration or timing from another manufacturer's method. Use the procedure validated for the BIOLAB reagent in your laboratory.

### How long should the slide stay in Hematoxylin?

Staining time depends on formulation, specimen, section thickness, reagent condition and laboratory procedure. Follow the current BIOLAB package instructions or validated SOP rather than using a generic timing.

### Can Harris Hematoxylin be used without Eosin?

Hematoxylin can be used as a nuclear stain in different procedures, but a conventional H&E stain requires both hematoxylin and eosin.

### Is this reagent ready to use?

The public BIOLAB catalogue confirms the product identity and staining application but does not provide enough detail to establish a universal preparation instruction for this exact 100 mL pack. Follow the current bottle label and package insert before use.

### Can the stain be filtered?

Laboratories should follow their validated SOP and manufacturer instructions regarding filtration, staining-bath maintenance and reagent reuse.

### How should Harris Hematoxylin be stored?

Store it according to the exact conditions printed on the current BIOLAB bottle label. Keep the container properly closed and protect the reagent from contamination.

### What is the expiry?

Expiry is batch-specific. Use the expiry printed on the supplied bottle. Customers requiring a specific minimum remaining shelf life should contact Microsidd before ordering.

### Is the reagent for home use?

No. BIOLAB Harris Hematoxylin is intended for professional laboratory staining procedures by appropriately trained personnel.

### Where can I buy BIOLAB Harris Hematoxylin in Bangalore?

BIOLAB Harris Hematoxylin 100 mL can be ordered through Microsidd.com subject to current stock. Microsidd India is based in RT Nagar, Bengaluru.

### Can hospitals and laboratories place bulk orders?

Bulk and institutional requirements may be supported depending on stock availability, quantity, required shelf life and applicable commercial terms.

### Does Microsidd deliver outside Bangalore?

Microsidd ships professional laboratory products to serviceable PIN codes across India, subject to current inventory and courier serviceability.

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## Professional Laboratory & Medical Disclaimer

**BIOLAB Harris Hematoxylin is intended for professional laboratory use by appropriately trained personnel.**

Information on this page is provided for product identification, procurement and general educational purposes and does not replace:

-   The current BIOLAB product label
-   Manufacturer Instructions for Use
-   Histology / cytology laboratory SOPs
-   Validated staining procedures
-   Specimen fixation and processing requirements
-   Quality-control procedures
-   Microscopy training and competency
-   Laboratory chemical-safety procedures
-   Professional pathological or cytological interpretation

A staining reagent does not independently establish a diagnosis. Microscopic findings must be interpreted by appropriately qualified professionals together with specimen quality, clinical information and other investigations where applicable.

If the current BIOLAB label or package documentation differs from general information displayed online, the current manufacturer documentation supplied with the reagent takes precedence.

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## No Return / No Exchange

**This laboratory reagent is non-returnable and non-exchangeable once correctly supplied.**

Please verify before ordering:

-   **Brand:** BIOLAB Diagnostics
-   **Product:** Hematoxylin (Harris)
-   **Pack Size:** 100 mL
-   Required staining formulation
-   Required H&E / PAP workflow
-   Laboratory application
-   Quantity
-   Storage requirements
-   Required remaining shelf life

Ordering the wrong stain formulation, brand, pack size or laboratory reagent is not considered a product defect where the supplied item matches the confirmed order.

Laboratory reagents may be affected by contamination, temperature, storage and handling after delivery. Correctly supplied reagents therefore cannot normally be accepted back into saleable inventory.

Genuine cases involving incorrect supply, verified transit damage, materially misdescribed products or other remedies required under applicable law will be handled according to the [**Microsidd Refund, Return, Replacement & Cancellation Policy**](https://microsidd.com/pages/cancellation-and-refunds).

**An original, continuous, unedited and uncut unboxing video is compulsory for delivery-related claims involving an incorrect, missing or damaged product.**

The video must begin before opening the parcel. Open the carton carefully by **cutting the sealing tape with a blade or appropriate cutter; do not tear open the carton**. Keep the courier label, sealed parcel, product label, bottle and condition continuously visible while opening.

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## BIOLAB Harris Hematoxylin 100ml – Product Entity Summary

**Product:** Hematoxylin (Harris)  
**Manufacturer Listing:** HEMATOXYLENE (HARRIS)  
**Brand:** BIOLAB Diagnostics  
**Pack Size:** 100 mL  
**Category:** Histology / Cytology Staining Reagent  
**Primary Function:** Nuclear Staining  
**Applications:** H&E and PAP Staining Workflows  
**Expected Staining:** Blue to Blue-Purple Nuclear Contrast following appropriate processing  
**Use:** Professional Laboratory Use Only  
**Typical Users:** Histopathology, Pathology, Cytology, Hospital, Teaching & Research Laboratories  
**Supplier:** Microsidd India  
**Supplier Location:** RT Nagar, Bengaluru, Karnataka, India

## Details

- **Price:** 499.0 INR - 2499.0 INR
- **Vendor:** Biolab
- **Type:** Histopathology / Cytology Stain
- **Tags:** biolab

## Variants

| Variant | Price | Available |
|---------|-------|-----------|
| 100ml | 499.00 INR | In stock |
| 500ml | 2499.00 INR | In stock |

## Images

- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.
- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.
- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.
- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.
- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.
- BIOLAB Harris Hematoxylin 100ml H&E PAP nuclear staining reagent.

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> Source: [Microsidd India](https://microsidd.com/products/biolab-hematoxylin-harris-100ml)
> Updated: 2026-11-06
